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An optimized method of culturing neurons based on polyacrylamide gel

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Biophysics Reports 2024年第1期10卷 41-47页

作者： Yongjing qiao Jihong Gong Ziqi Jin Yiting Tu Xiaofei yang Key Laboratory of Cognitive Science Hubei Key Laboratory of Medical Information Analysis and Tumor Diagnosis & Treatment Laboratory of Membrane Ion Channels and Medicine College of Biomedical Engineering SouthCentral Minzu University

Substrate stiffness is a microenvironment with a certain stiffness constructed by the extracellular matrix and adjacent cells, which plays an important role in the growth and development of cells and tissue formation. Studies have indicated that the stiffness of the brain is about 0.1–1 kPa. The physiological and pathological processes of the nervous system are mediated by the substrate stiffness that the neurons suffer. However, how substrate stiffness regulates these processes remains to be studied. Culturing neurons on substrates with different stiffness in vitro is one of the best methods to study the role of stiffness in regulating neuronal development and activity. In this study, by changing the preparation time and the activation time of polyacrylamide gel, we provide an improved method that achieves a low toxic substrate environment for better primary neuron adhesion and development. Hope that this method is convenient for those studying the role of substrate stiffness in neurons.

关键词： Polyacrylamide gel Neuron culture Cell adhesion Neural cell development

Regio-and Diasteroselectivity of Rhodium-catalyzed Ring Opening Reaction of Oxabenzonorbornadienes with Heteroatom Nucleophiles

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Chinese Chemical Letters 2003年第7期14卷 697-699页

作者： Ding qiao yang He Ping ZENG Department of Chemistry South China Normal UniversityGuangzhou 510631

A new rhodium catalyzed ring opening reaction of oxabenzonorbornadienes and its derivatives was described. This reaction forms a new carbon-nitrogen bond via an intermolecular allylic displacement of the bridgehead o... 详细信息

关键词： Ring opening rhodium-catalyzed oxabenzonorbornadienes heteroatom nucleophiles.

Weight change and all-cause and cause-specific mortality:A 25-year follow-up study

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Chinese Medical Journal 2024年第10期137卷 1169-1178页

作者： Huan yang Jianbing Wang Xiaokun Wang Wanyi Sun Chenyunhao Tong Jinhu Fan Youlin qiao Christian *** Department of Cancer Epidemiology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Department of Public Health Zhejiang University School of MedicineNational Clinical Research Center for Children’s HealthHangzhouZhejiang 310058China Metabolic Epidemiology Branch Division of Cancer Epidemiology and GeneticsNational Cancer InstituteRockvilleMD 20850USA

Background:Whether the dynamic weight change is an independent risk factor for mortality remains *** study aimed to examine the association between weight change and risk of all-cause and cause-specific mortality based on the Linxian Nutrition Intervention Trial(NIT)***:Body weight of 21,028 healthy residents of Linxian,Henan province,aged 40-69 years was measured two times from 1986 to *** events were prospectively collected up to *** maintenance group(weight change<2 kg)or stable normal weight group was treated as the *** proportional hazard model was performed to calculate hazard ratios(HRs)and 95%confidence intervals(95%CIs)to estimate the risk of ***:A total of 21,028 subjects were included in the final *** with the weight maintenance group,subjects with weight loss≥2 kg had an increased risk of death from all-cause(HR_(All-cause)=1.14,95%CI:1.09-1.19,P<0.001),cancer(HR_(Cancer)=1.12,95%CI:1.03-1.21,P=0.009),and heart disease(HR_(Heart diseases)=1.21,95%CI:1.11-1.31,P<0.001),whereas subjects with weight gain≥5 kg had 11%(HR_(Cancer)=0.89,95%CI:0.79-0.99,P=0.033)lower risk of cancer mortality and 23%higher risk of stroke mortality(HR_(Stroke)=1.23,95%CI:1.12-1.34,P<0.001).For the change of weight status,both going from overweight to normal weight and becoming underweight within 5 years could increase the risk of total death(HR_(Overweight to normal)=1.18,95%CI:1.09-1.27;HR_(Becoming underweight)=1.35,95%CI:1.25-1.46)and cancer death(HR_(Overweight to normal)=1.20,95%CI:1.04-1.39;HR_(Becoming underweight)=1.44,95%CI:1.24-1.67),while stable overweight could increase the risk of total death(HR_(Stable overweight)=1.11,95%CI:1.05-1.17)and death from stroke(HR_(Stable overweight)=1.44,95%CI:1.33-1.56).Interaction effects were observed between age and weight change on cancer mortality,as well as between baseline BMI and weight change on all-cause,heart disease,and stroke mortality(all P_(

关键词： Weight change All-cause mortality Cancer Stroke Heart diseases Body mass index

液相色谱-串联质谱法测定花椒中的6种山椒素及其产地鉴别

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食品安全质量检测学报 2024年第13期15卷 29-40页

作者：杨巧慧张宇谷学权文堂军陈亚李霞雪幸勇曾艳雅安市农产品质量监测检验中心雅安625000 四川五丰黎红食品有公司雅安625000

目的建立液相色谱-串联质谱法(liquid chromatography tandem mass spectrometry,LC-MS/MS)快速测定花椒中6种山椒素的方法 ,并进行产地分析。方法样品通过甲醇超声提取,LC-MS/MS外标法测定,对提取方式、仪器条件进行了优化,与高效液... 详细信息

目的建立液相色谱-串联质谱法(liquid chromatography tandem mass spectrometry,LC-MS/MS)快速测定花椒中6种山椒素的方法 ,并进行产地分析。方法样品通过甲醇超声提取,LC-MS/MS外标法测定,对提取方式、仪器条件进行了优化,与高效液相色谱法(high performance liquid chromatography,HPLC)检测进行比较,对方法正确度、精密度、检出限和定量限进行了验证。利用该方法对不同产地的10个青花椒、33个红花椒进行测定,通过聚类分析、主成分分析(principal component analysis,PCA)进行产地分析鉴别。结果 6种山椒素在LC-MS/MS上分离良好,在0.10~5.00μg/m L范围内线性关系良好,相关系数(r^(2))≥0.996,检出限为0.10×10^(–3)~0.18×10^(–3) mg/g,定量限为0.33×10^(–3)~0.61×10^(–3) mg/g;在低、中、高3个水平下6种山椒素的平均回收率在86.67%~100.67%之间,相对标准偏差为0.72%~9.11%,正确度和精密度均符合方法验证要求,检出限、定量限均低于HPLC方法 ,灵敏度、准确度更高。通过对不同产地花椒的检测结果显示,羟基-α-山椒素为含量最高的麻味物质,且两类花椒测定结果均表现出汉源花椒中6种山椒素总量高于其他产地花椒的特点。通过聚类分析、PCA分析表明,汉源青花椒中羟基-β-山椒素,红花椒中羟基-α-山椒素、羟基-γ-山椒素、γ-山椒素含量均高于所测其他产地同种花椒中麻味物质含量,是区别于其他产地花椒的主要麻味物质。结论该方法前处理简单,灵敏度、准确度高,可用于花椒中6种山椒素的检测,且通过对花椒中6种山椒素的检测及聚类、PCA分析能有效区分汉源花椒与其他产地花椒,为鉴别真假汉源花椒提供了可靠的方法。

关键词：液相色谱-串联质谱法山椒素汉源花椒聚类分析主成分分析

Cu/Mo2C synthesized through Anderson-type polyoxometalates modulate interfacial water structure to achieve hydrogen evolution at high current density

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Nano Research 2024年第4期17卷 2546-2554页

作者： Dunyuan Jin Fen qiao Yan Zhou Junfeng Wang Kecheng Cao Jing yang Jikang Zhao Lei Zhou Haitao Li School of Energy and Power Engineering Jiangsu UniversityZhenjiang 212013China School of Physical Science and Technology and Shanghai Key Laboratory of High-resolution Electron Microscopy ShanghaiTech UniversityShanghai 201210China Institute for Energy Research Jiangsu UniversityZhenjiang 212013China

The development of efficient non-precious metal catalysts is important for the large-scale application of alkaline hydrogen evolution reaction(HER).Here,we synthesized a composite catalyst of Cu and Mo_(2)C(Cu/Mo_(2)C)using Anderson-type polyoxometalates(POMs)synthesized by the facile soaking method as *** electronic interaction between Cu and Mo_(2)C drives the positive charge of Cu,alleviating the strong adsorption of hydrogen at the Mo site by modulating the d-band center of Mo_(2)*** studying the interfacial water structure using in situ attenuated total reflection surface-enhanced infrared absorption spectroscopy(ATR-SEIRAS),we determined that the positively charged Cu crystals have the function of activating water molecules and optimizing the interfacial water *** interfacial water of Cu/Mo_(2)C contains a large amount of free water,which could facilitate the transport of reaction *** to activated water molecules and optimized interfacial water structure and hydrogen adsorption energy,the overpotential of Cu/Mo_(2)C is 24 mV at a current density of 10 mA·cm^(-2) and 178 mV at a current density of 1000 mA·cm^(-2).This work improves catalyst performance in terms of interfacial water structure optimization and deepens the understanding of water-mediated catalysis.

关键词： electrocatalytic hydrogen production interfacial water charge transport d band center in situ attenuated total reflection surface-enhanced infrared absorption spectroscopy(ATR-SEIRAS)

Energy partition in laser-induced cavitation bubbles near the rigid wall with a gas-containing hole

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Journal of Hydrodynamics 2024年第3期36卷 435-443页

作者： Dong-qiao He Hai-gang Wen Si-yuan Geng Chen-xi yang Qiang Zhong Zhi-feng Yao College of Water Resources and Civil Engineering China Agricultural UniversityBeijing100083China PowerChina Beijing Engineering Corporation Limited Beijing100024China State Key Laboratory of Efficient Utilization of Agricultural Water Resources Beijing100083China Beijing Engineering Research Center of Safety and Energy Saving Technology for Water Supply Network System China Agricultural UniversityBeijing100083China

To investigate the energy partition in laser-induced cavitation bubbles near the rigid wall with a gas-containing hole,we utilized a nanosecond resolution photography system based on a Q-switched Nd:YAG laser and conventional industrial camera to carefully observe the transient process of bubble collapse near the rigid wall with a gas-containing *** analyzed the generation of collapse microjets and the emission of collapse shock *** found that the cavitation bubble near the rigid wall with a gas-containing hole collapsed at different times and space,and produced various types of shock *** on the far field pressure information of the shock waves measured by hydrophone,the energy of the shock waves generated by the bubble collapse near the rigid wall with a gas-containing hole is calculated for the first *** results show that the ratio of collapse shock wave energy to bubble energy is approximately between 0.7 and 0.8.

关键词： Energy partition bubble collapse laser-induced shock wave gas-containing hole

GPa-level pressure-induced enhanced corrosion resistance in TiZrTaNbSn biomedical high-entropy alloy

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China Foundry 2024年第3期21卷 265-275页

作者： Xiao-hong Wang Yu-lei Deng qiao-yu Li Zhi-xin Xu Teng-fei Ma Xing yang Duo Dong Dong-dong Zhu Xiao-hong yang Key Laboratory of Air-driven Equipment Technology of Zhejiang Province Quzhou UniversityQuzhou 324000ZhejiangChina School of Materials Science and Engineering Taizhou UniversityTaizhou 318000ZhejiangChina

TiZrTaNb-based high-entropy alloys(HEAs)are research frontier of biomedical materials due to their high hardness,good yield strength,excellent wear resistance and corrosion ***,as an essential trace element in the human body that plays a significant role in physiological *** has stable chemical properties and a low elastic *** this study,a new material,TiZrTaNbSn HEAs,was proposed as a potential biomedical *** Ti_(35)Zr_(25)Ta_(15)Nb_(15)Sn_(10)biomedical high-entropy alloys(BHEAs)were successfully prepared through an arc melting furnace and then remelted using a German high-temperature and high-pressure apparatus under GPa-level(4 GPa and 7 GPa).The precipitation behavior of the needle-like HCP-Zr_(5)Sn_(3)phase that precipitates discontinuously at the grain boundary was successfully *** phase constitution,microstructure,and corrosion resistance of the alloy were *** results show that the needle-like HCP-Zr_(5)Sn_(3)phase is eliminated and the(Zr,Sn)-rich nano-precipitated phase is precipitated in the microstructure under high pressure,which leads to the narrowing of grain boundaries and consequently improves the corrosion resistance of the *** addition,the formation mechanisms of(Zr,Sn)-rich nanoprecipitates in BHEAs were *** Zr and Sn dissolve in the matrix due to the effect of high pressure,during the cooling process,they precipitate to form a(Zr,Sn)-rich nano-precipitated phase.

关键词： biomedical HEAs precipitation grain boundary corrosion resistance TiZrTaNbSn

Microstructural evolution and deformation behavior of friction stir welded twin-induced plasticity steel

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Journal of Materials Science & Technology 2024年第2期169卷 68-81页

作者： Ke qiao Kuaishe Wang Jia Wang Zhengyang Hao Yating Xiang Peng Han Jun Cai Qi yang Wen Wang School of Metallurgical Engineering National and Local Joint Engineering Research Center for Functional Materials ProcessingXi’an University of Architecture and TechnologyXi’an 710055China Shanghai Research Institute of Materials Shanghai 200437China

The weldability of twin-induced plasticity(TWIP)steel with ultra-high strength via friction stir welding(FSW)technique was investigated,and microstructural evolution and deformation behavior of whole and micro-zones of FSW TWIP joint were studied for the first *** results showed that the content of recrystallized grains in the stir zone(SZ)increased from 10.5%of basal material(BM)to 14.2%,and that of heat affected zone(HAZ)increased to 78.6%.The percentage of annealing twins decreased from 26.8%in BM to 11%in SZ,while increased to 35%in *** with the BM,the ultimate tensile strength and yield strength of the FSW joint increased to 1036 and 550 MPa,respectively,reaching 106.7%and 110.9%of BM,*** elongation of the entire joint was 50.5%,which was lower than that of BM due to the nonuniform deformation during the tensile *** engineering strain was mainly concentrated in BM and SZ and transferred to each other during the tensile test,while the engineering strain in HAZ was always the ***,the tensile fracture occurred in the *** order of ultimate tensile strength of micro-zones in the FSW joint was as follows:HAZ>BM≈*** order of yield strength was as follows:HAZ>BM>SZ.

关键词： Twin-induced plasticity steel Friction stir welding Microstructure Mechanical property Deformation behavior

Efficacy of surgical resection and ultra-reduced tension suture combined with superficial radiation in keloid treatment

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World Journal of Clinical Cases 2023年第35期11卷 8310-8319页

作者： Xiao-Ying Hu qiao yang Xiao-Yu Guan Jin-Ying Li Ling-Ling Wang Kun Li Xiao-Tao Zhang Department of Cosmetic Medicine Qingdao Eighth People’s HospitalQingdao 266100Shandong ProvinceChina Department of Radiation Oncology The Affiliated Qingdao Central Hospital of Qingdao UniversityQingdao 266042Shandong ProvinceChina

BACKGROUND There are many available treatment options for keloid;however,single treatments are usually less ***,more scientifically rational and effective com-bined treatment methods should be sought to solve the pain associated with *** To explore the efficacy and safety of surgical resection and ultra-reduced tension suture combined with superficial radiation as keloid *** Fifteen keloid patients admitted to Qingdao Eighth People's Hospital from June 2020 to January 2022 were enrolled in this retrospective *** patients underwent a comprehensive treatment approach comprising surgical resection,ultra-reduced tension suture incision,and superficial radiation therapy within 24 h *** modified Vancouver Scar Scale(mVSS)and Patient and Observer Scar Assessment Scale(POSAS)were used to evaluate the treatment effect,whereas the efficacy,adverse effects,and recurrence rate were observed according to the 12-mo follow-up after *** The mVSS and POSAS scores at 1 and 6 mo after combination treatment decreased compared to before treatment(P<0.001),and the overall response rate was 93.3%.Only one case recurred,yielding a 6.7%recurrence *** incidence of local chromour sedimentation rate in 1–3 mo after radiotherapy was 33.3%(5 patients),all subsiding after 6–9 mo,without complications,such as delayed wound healing or *** Surgical resection,super subtraction sutures,and superficial radiotherapy are treatment methods with short courses,low recurrence rates,and good safety profiles.

关键词： Keloid Ultrasound suture Radiotherapeutics Superficial radiation Ultra-reduced tension suture Radiation