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Effect of hormonal status and metabolic changes of restricted ewes during late pregnancy on their fetal growth and development

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science China(Life sciences) 2007年第6期50卷 766-772页

作者： GAO Feng1, HOU XianZhi1 & LIU YingChun2 1 College of animal science and animal medicine, inner mongolia agricultural university, huhhot 010018, China 2 College of Biotechnology and Bioengineering, inner mongolia agricultural university, huhhot 010018, China College of animal science and animal medicine Inner Mongolia Agricultural University Huhhot China College of Biotechnology and Bioengineering Inner Mongolia Agricultural University Huhhot China

This study investigated the effects of hormonal status; metabolic changes of restricted ewes during late pregnancy on the ovine fetus growth; development. One hundred mongolian ewes, synchronized for oestrus; mated, were divided into three groups; offered 0.175 MJME·kgw[0.75]·d[1] (Restricted Group 1, RG1), 0.33 MJME·kgw[0.75]·d[1] (Restricted Group 2, RG2); ad libitum access to feed (Control Group, CG) during their late pregnancy respectively. The results suggested that with the supply of exogenous energy decreasing during late pregnancy, maternal body weight; net body weight loss in RG2; RG1 were lower than those of CG (Ps0.01). The insulin; IGF-1 concentrations of ewes in RG2; RG1 tended to be lower than those of CG (P>0.05), but the GH concentrations in RG2; RG1 were enhanced; there was significant difference between RG1; CG on d 120 of gestation (P0.05). However, the NEFA; FAA concentrations of ewes in RG1 were reduced from d 90 to d 120 of gestation, then enhanced from d 120 to d 140 of gestation. During the late pregnancy, with the supply of nutrition decreasing, the negatively physiological; biochemical maternal reactions to restriction became worse, which significantly reduced the average lamb birth weight; daily growth rate of fetus in RG2 (P<0.05); RG1 (P<0.01).

关键词： late pregnancy restricted ewes fetus growth development

Study of Domestic animals Lice in inner mongolia Autonomous Region,China

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Study of Domestic Animals Lice in Inner Mongolia Autonomous ...

中国动物学会第七届全国青年寄生虫学工作者学术讨论会

作者： xiaoye yang Lianru yang & Zhenlian Liu(College of animal science and animal medicine.inner mongolia agricultural university.huhhot 010018 China)

＜正＞ This paper reported 17 lice species of domestic animals in inner mongolia Region, China. Eight species were sucking lice （Anoplura） of families Haematopinidae and Linognathidae. Three species of genus Haematopinus, in family Haematopinidae, were H. suis from pigs, H. eurysternus from cattle, and H asini from horses. Family Linognathidae is

关键词： Domestic animal Lice Species inner mongolia China

Identification of patrilineal genetic variation in Chinese indigenous horses through Y-chromosome microsatellites

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Identification of patrilineal genetic variation in Chinese i...

第十五次全国动物遗传育种学术讨论会

作者： LING Yinghui~(1,2),MA Yuehui~(1,2),GUAN Weijun~(1,2),CHENG Yuejiao~(1,2),HAN Jianlin~(1,2),MANG Lai~3 1.Institute of animal science,Chinese Academy of agricultural sciences,Beijing 100193 2.The Key Laboratory for Farm animal Genetic Resources and Utilization of Ministry of Agriculture,Beijing 100193 3.College of animal science and Veterinary medicine.inner mongolia agricultural university.huhhot 010018

Introduction The entire Y chromosome except the pseudoautosomal region acts as a single non-recombining unit,which is male specific and effectively *** ensures that original combinations of mutational events along male lineages are preserved as single-linked unambiguous haplotypes.Y chromosome polymorphisms such as microsatellites represent

关键词： horse Y-chromosome haplotype microsatellite phylogeography

Cloning,expression and immunocompetence analysis of gene encoding the glutamate dehydrogenase of Streptococcus suis serotype 2

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Cloning,expression and immunocompetence analysis of gene enc...

中国畜牧兽医学会畜牧兽医生物技术学分会暨中国免疫学会兽医免疫分会第八次学术研讨会

作者： yang Wei-jun~(1,3*),yang Zhi-biao~(1*),CAI Xue-hui~2,HAO Yong-qing~3,ZHENG Sheng-bo~1, LAN Dao-liang~1,GU Jiang-ping~1,XING Rui~1,YUAN Cong-li~1,CUI Li~1,HUA Xiu-guo~1 (1.Shanghai Key Laboratory of Veterinary Biotechnology,College of agricultural and Biology,Shanghai Jiaotong university, Shanghai 200240,China 2.Division of Swine Infectious Diseases,State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of agricultural sciences,Harbin 150001,China 3.College of animal science and animal medicine.inner mongolia agricultural university.huhhot 010018,China)

This study aimed to study the immune activity of glutamate dehydrogenase(GDH) of streptococcus suis serotype 2 and detect its ***:The GDH gene was cloned and *** GDH protein was expressed in a prokaryotic expression system and purified by affinity *** of the resulting protein was analyzed by western blot with anti-GDH monoclonal *** fusion protein was efficiently *** blot analysis confirmed that the resulting protein was *** GDH gene of *** 2 was successfully cloned and the high expressed,which facilitated the further studies on the development of immunological detection and vaccine design.

关键词： Streptococcus suis serotype 2 GDH prokaryotic expression Immunocompetence

Substitutions of stem-loop subdomains in internal ribosome entry site of Senecavirus A:Impacts on rescue of sequence-modifying viruses

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Journal of Integrative Agriculture 2024年第7期23卷 2391-2406页

作者： Qianqian wang Jie wang Lei Zhang Xiaoxiao Duan Lijie Zhu Youming Zhang Yan Li Fuxiao Liu College of Veterinary medicine Qingdao Agricultural UniversityQingdao 266109China College of Veterinary medicine Inner Mongolia Agricultural UniversityHuhhot 010018China Shandong New Hope Liuhe Group Co. Ltd.Qingdao 266100China Qingdao Center for animal Disease Control&Prevention Qingdao 266199China State Key Laboratory of Microbial Technology Shandong UniversityQingdao 266237China

Senecavirus A(SVA)has a positive-sense,single-stranded RNA *** 5´untranslated region harbors an internal ribosome entry site(IRES),comprising 10 larger or smaller stem-loop structures(including a pseudoknot)that have been demonstrated to be well ***,it is still unclear whether each stem-loop subdomain,such as a single stem or loop,is also highly *** clarify this issue in the present study,a set of 29 SVA cDNA clones were constructed by site-directed mutagenesis(SDM)on the *** SDM-modified scenarios included:(1)stem-formed complementary sequences exchanging with each other;(2)loop transversion;(3)loop transition;and(4)point *** cDNA clones were separately transfected into cells for rescuing viable viruses,whereas only four SVAs of interest could be recovered,and were genetically stable during 20 *** progeny grew significantly slower than the other three *** dual-luciferase reporter assay showed that none of the SDM-modified IRESes significantly inhibited the IRES *** previous study indicated that a single motif from any of the ten stem structures,if completely mutated,would cause the failure of virus ***,our present study revealed three stem structures,whose individual complementary sequences could exchange with each other to rescue sequence-modifying ***,one apical loop was demonstrated to have the ability to tolerate its own full-length transition,also having no impact on the recovery of sequence-modifying *** present study suggested that not every stem-loop structure was strictly conserved in its conformation,while the full-length IRES itself was well *** provides a new research direction on interaction between the IRES and many factors.

关键词： SVA HCV IRES HCV-like IRES stem-loop structure cDNA clone virus rescue mutation

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猪人工授精技术在我国北方地区的应用

四川畜牧兽医 2010年第1期37卷 27-29页

作者：乌仁图娜勒梁天义姜向宏李东兴郭少平丁庆庆商晓桂内蒙古鄂尔多斯市鄂托克旗家畜改良工作站内蒙古鄂尔多斯016100 内蒙古农业大学动物科学与医学学院内蒙古呼和浩特010018

猪人工授精技术是当前养猪业生产中的一个重要环节,在我国北方地区,尤其是内蒙古地区,猪人工授精技术还没有普及。为了适应养猪业发展步伐,在内蒙古西部地区的养猪场建立了采精站,针对北方冬季寒冷的特点,探索在采精室安置暖气和空调,... 详细信息

猪人工授精技术是当前养猪业生产中的一个重要环节,在我国北方地区,尤其是内蒙古地区,猪人工授精技术还没有普及。为了适应养猪业发展步伐,在内蒙古西部地区的养猪场建立了采精站,针对北方冬季寒冷的特点,探索在采精室安置暖气和空调,授精时用护腕包裹授精瓶等前提下应用猪人工授精技术,达到促进我国北方地区养猪业健康良性快速发展的目的。

关键词：人工授精采精室采精妊娠检查

牛乳水解蛋白在酶-膜反应器中连续化生产及其动物消化实验研究

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食品科技 2010年第7期35卷 70-73页

作者：陈静陈玉香张和平孙健北京物资学院物流学院北京101149 吉林大学生物与农业工程学院长春130022 内蒙古农业大学食品科学与工程学院呼和浩特010018 蒙牛乳业(北京)有限责任公司通州101107

实验采用中空纤维超滤器(MB10KD)、保温系统及回流系统组建形成小型连续化酶-膜反应器;采用胰蛋白酶作为水解用酶,连续化制备牛乳水解蛋白。通过对得到的水解蛋白进行小鼠动物消化实验,发现利用连续化酶-膜反应器水解制备的水解蛋白在... 详细信息

实验采用中空纤维超滤器(MB10KD)、保温系统及回流系统组建形成小型连续化酶-膜反应器;采用胰蛋白酶作为水解用酶,连续化制备牛乳水解蛋白。通过对得到的水解蛋白进行小鼠动物消化实验,发现利用连续化酶-膜反应器水解制备的水解蛋白在实验小鼠的胃液及肠液中的三氯乙酸可溶性氨基氮含量最高,实验结果表明此牛乳水解蛋白在小鼠胃中、肠中的消化率均明显高于原牛乳以及传统的婴儿配方乳。

关键词：酶-膜反应器消化率可溶性氨基氮婴儿配方乳

INDUSTRIALIZATION OF BEEF EMBRYO TRANSFER IN CHINA

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INDUSTRIALIZATION OF BEEF EMBRYO TRANSFER IN CHINA

面向21世纪的动物科学与动物医学国际研讨会

作者： LiShuJing, YuWenLi, Wu De Guo, NaDaMuDeinner mongolia Livestock Improvement Station, huhhot, 010010 Genetic Institution, Chinese Academy of science, Beijing, 100101

＜正＞In China, there are about 0.1 billion local cattle that need to be improved because of their poor qualities in production and that can be selected as the rich source of recipients for MOET. The remarkable differences in production performance between the local and imported breed makes MOET have extremely important significance in China. In view of the reason, the MOET industrualization project have been being conducted since 1990. According to incom-plete statistics, so far, total 14000 embryos have been produced, over 35000 beef embryos have been imported, and about 52000 recipients have been transferred. The data related are as following:

关键词： beef embryo transfer industrialization

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细菌人工染色体基因组文库构建关键技术研究

生物技术通报 2008年第4期24卷 66-69页

作者：刘长青吴宏梅包阿东陆涛峰刘帅张洪海唐学玺关伟军马月辉中国农业科学院北京畜牧兽医研究所曲阜师范大学研究生处曲阜273165

基因组文库是进行分子克隆和基因结构与功能研究的基础,完整覆盖的基因组文库的构建,使基因组任何DNA片段的筛选和获得成为可能。细菌人工染色体(Bacterial Artificial Chromosome,BAC)与其它载体系统相比具有转化效率高、嵌合体少、插... 详细信息

基因组文库是进行分子克隆和基因结构与功能研究的基础,完整覆盖的基因组文库的构建,使基因组任何DNA片段的筛选和获得成为可能。细菌人工染色体(Bacterial Artificial Chromosome,BAC)与其它载体系统相比具有转化效率高、嵌合体少、插入片段易回收,高覆盖率、稳定性强,并且具有易分离和操作等特性等优点而被广泛应用。作为物种保护策略的重要部分,构建我国濒危动植物品种基因组BAC文库,对于其遗传资源的保护和研究具有非常重要的作用。在查阅大量国内外相关资料的基础上,就BAC基因组文库构建过程中载体的制备、高分子量DNA的制备、大片段DNA的回收、连接与电击转化、基因组BAC文库质量鉴定等几个重点、难点问题进行了详细的论述和分析,对于构建高分子量插入片段、高覆盖率和稳定性的基因组文库提供理论基础与技术支撑。

关键词：细菌人工染色体基因组文库构建质粒纯化高分子量DNA