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Lactobacillus reuteri-derived extracellular vesicles maintain intestinal immune homeostasis against lipopolysaccharideinduced inflammatory responses in broilers

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Journal of Animal Science and Biotechnology 2021年第3期12卷 1196-1213页

作者： Rujiu Hu Hua Lin Mimi Wang Yuezhen Zhao Haojing Liu Yuna Min xiaojun yang Yupeng Gao Mingming yang College of Animal Science and Technology Northwest A&F UniversityYangling 712100ShaanxiChina

Background:Lactobacillus reuteri strains are widely used as probiotics to prevent and treat inflammatory bowel disease by modulating the host’s immune ***,the underlying mechanisms by which they communicate with the host have not been clearly *** extracellular vesicles(EVs)have been considered as important mediators of host-pathogen interactions,but their potential role in commensals-host crosstalk has not been widely ***,we investigated the regulatory actions of EVs produced by *** BBC3,a gut-associated commensal bacterium of Black-Bone chicken,in the development of lipopolysaccharide(LPS)-induced intestinal inflammation in a chicken model using both in vivo and in vitro ***:*** BBC3 produced nano-scale membrane vesicles with the size range of 60-250 *** and proteomic analyses showed that *** BBC3-derived EVs(LrEVs)carried DNA,RNA and several bioactive proteins previously described as mediators of other probiotics’beneficial effects such as glucosyltransferase,serine protease and elongation factor *** vivo broiler experiments showed that administration of LrEVs exerted similar effects as *** BBC3 in attenuating LPS-induced inflammation by improving growth performance,reducing mortality and decreasing intestinal *** suppressed the LPS-induced expression of pro-inflammatory genes(TNF-α,IL-1β,IL-6,IL-17 and IL-8),and improved the expression of anti-inflammatory genes(IL-10 and TGF-β)in the *** could be internalized by chicken *** vitro pretreatment with LrEVs reduced the gene expression of TNF-α,IL-1βand IL-6 by suppressing the NF-κB activity,and enhanced the gene expression of IL-10 and TGF-βin LPS-activated chicken ***,LrEVs could inhibit Th1-and Th17-mediated inflammatory responses and enhance the immunoregulatory cells-mediated immunosuppression in splenic lymphocytes of LPS-challenged chickens through the activation of macro

关键词： Chickens Extracellular vesicles Immune responses Inflammation Lactobacillus Microbiota-host communication Probiotics

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Dietary folic acid addition reduces abdominal fat deposition mediated by alterations in gut microbiota and SCFA production in broilers

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Animal Nutrition 2023年第1期12卷 54-62页

作者： Yanli Liu Jiantao yang Xiaoying Liu Rui Liu Yibin Wang Xinhuo Huang Yingge Li Ruifang Liu xiaojun yang College of Animal Science and Technology Northwest A&F UniversityYangling 712100China Nano Vitamin Engineering Research Center of Shaanxi Province Xi'an 710000China Shaanxi Province Animal Husbandry Technology Extension Station Xi'an 710016China

Intensive selective breeding for high growth rate and body weight cause excess abdominal fat in *** microbiota and folic acid were reported to regulate lipid metabolism.A total of 210 one-day-old broilers were divided into the control(folic acid at 1.3 mg/kg)and folic acid groups(folic acid at 13 mg/kg)to illustrate the effects of folic acid on growth performance,abdominal fat deposition,and gut microbiota,and the experiment lasted 28 *** revealed that dietary folic acid addition decreased abdominal fat percentage(P<0.05)and down-regulated genes expression related to cell proliferation and differentiation in abdominal fat including IGF1,EGF,C/EBPα,PPARγ,PLIN1,FABP4 and PCNA(P<0.05).Folic acid addition decreased caecal Firmicutes-to-Bacteroidetes ratio(P<0.01)and increased the proportions of Alistipes,Oscillospira,Ruminococcus,Clostridium,Dehalobacterium and Parabacteroides(P<0.05).Caecal acetic acid,and propionic acid contents were found to be higher under folic acid treatment(P<0.05),which were negatively related to genes expression associated with adipocyte proliferation and differentiation(P<0.05).Ruminococcus was positively correlated with caecal acetic acid content,and the same phenomenon was detected between propionic acid and Oscillospira and Ruminococcus(P<0.05).Acetic acid and Oscillospira were identified to be negatively associated with abdominal fat percentage(P<0.05).In conclusion,our data demonstrated that dietary supplementation of folic acid reduced fat deposition in broilers by inhibiting abdominal adipocyte proliferation and differentiation,which might be mediated by changes in gut microbiota and short chain fatty acid production.

关键词： Folic acid Caecal microbiota Abdominal adipocytes Short chain fatty acid Broilers

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Wood plastic composites based wood wall’s structure and thermal insulation performance

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Journal of Bioresources and Bioproducts 2021年第1期6卷 65-74页

作者： Lu Zhang Zehua Chen Haoran Dong Shuai Fu Lan Ma xiaojun yang College of Material Science and Engineering Nanjing Forestry UniversityNanjing 210037China

In order to solve the problem of poor thermal insulation in the current wood-plastic building,two kinds of structural wood wall integrated with wood plastic composite(WPC)are designed,and the thermal insulation performances of the walls are *** results show that the WPC integrated wall with frame-shear structure has a good stability,and the excellent performance of the WPC can be fully *** studs and wall panels are important factors affecting the thermal performance of the *** plastic materials can meet the thermal performance requirements of the *** single-layer frame walls and double-layer frame walls integrated with the WPC both have a good thermal *** to‘Design Standard for Energy Efficiency of Public Buildings(GB 50189-2015)’,the heat transfer coefficient of the single-layer frame wall integrated with 20 mm thick WPC wall boards and WPC wall studs is 0.414 W/(m^(2)•K),which can meet the standard of wall thermal levelⅡt and is suitable for cold *** heat transfer coefficient of the double-layer frame wall integrated with 50 mm thick WPC wall panel and WPC wall studs is 0.207 W/(m^(2)•K),which can meet the standard of wall thermal levelⅠt and is suitable for severe cold areas.

关键词： Wood plastic composite(WPC) Thermal insulation performance Heat transfer coefficient Frame-shear structure wall

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Enhanced superconductivity in hole-doped Nb2PdS5

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Frontiers of physics 2017年第5期12卷 57-62页

作者： Qian Chen Xiaohui yang xiaojun yang Jian Chen Chenyi Shen Pan Zhang Yupeng Li Qian Tao Zhu-An Xu Department of Physics and State Key Laboratory of Silicon Materials Zhejiang University Hangzhou 310027 China Zhejiang California International NanoSystems Institute Zhejiang University Hangzhou 310058 China Collaborative Innovation Centre of Advanced Microstructures Nanjing 210093 China

We synthesized a series of Nb2Pd1-xRuxS5 polycrystalline samples by a solid-state reaction method and systematically investigated the Ru-doping effect on superconductivity by transport and magnetic measurements. It is found that superconductivity is enhanced with Ru doping and is quite robust upon disorder. Hall coefficient measurements indicate that the charge transport is dominated by hole-type charge carriers similar to the case of Ir doping, suggesting multi-band superconductivity. Upon Ru or Ir doping, Hc2/Tc exhibits a significant enhancement, exceeding the Pauli paramagnetic limit value by a factor of approximately 4. A comparison of Tc and the upper critical field （Hc2） amongst the different doping elements on Pd site, reveals a significant role of spin-orbit coupling.

关键词： superconductivity hole-doping upper critical field spin-orbit coupling phase diagram

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Status and distribution changes of the endangered Green Peafowl (Pavo muticus) in China over the past three decades (1990s-2017)

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Avian Research 2018年第2期9卷 102-110页

作者： Dejun Kong Fei Wu Pengfei Shan Jianyun Gao Dao Yan Weixiong Luo xiaojun yang Key Laboratory of Special Biological Resource Development and Utilization of Universities in Yunnan Province Kunming University Kunming 650214 China Bird Group Kunming Institute of Zoology Chinese Academy of Sciences Kunming 650223 China Kunming Survey and Design Institute of State Forestry Administration Kunming 650216 China

Background： Understanding the changes in population dynamics, including demographics, distribution and threats is essential for species status *** endangered Green Peafowl （Povo muticus） has experienced sharp population declines and d stribution range diminishing both in China and Southeast Asia. Field population surveys have not been conducted in China since the 1990s, which hindered conservation planning and ***： With interview and line transects methods, we figured out the population and distribution changes of Green Peafowl across its historical ranges over the past three decades in China during ***： The Green Peafowl once habituated in 54 counties in China. Nearly 60% of the distribution counties were lost in the past three decades, with the left 22 counties distributed in central, southern and western Yunnan, SW China. Population decrease detected in all distribution areas except for Shuangbai and Xinping county where more than 60% of the total population is located. Only about 30% of the former bird population were recorded with the same interviewing method as 20 years *** birds, 1 carcass, 6 calls and 12 footprints were detected along the 865 km line transects, indicating extremely low encounter rate of Green Peafowl in field. Sharp decreases in flock sizes were also detected, from 8-20 birds per flock in the 1990s to 3-5 birds at present. Poaching and habitat conversion are two widespread and long-lasting threats, while poison tion affect regional population＇s survival. Large flocks of 18 ng caused mortality in the past and hydropower construc -27 birds were discovered in the field, which increases our confidence of population recovery of this endangered pheasant in ***： Only interviewed bird number and counts based on line transects were presented in this study, without further population estimation due to limitation of the data sets. Although the actual population of this cryptic bird must be

关键词： Green Peafowl Population decline Range concentration Endangered species Southwestern China

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An Improved Artificial Neural Network Model for Predicting Silicon Content of Blast Furnace Hot Metal

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International Journal of Minerals,Metallurgy and Materials 2000年第4期14卷 269-272页

作者： Bin Yao, Tianjun yang, xiaojun Ning (Metallurgy School, University of Science and Technology Beijing, Beijing 100083, China) Metallurgy School University of Science and Technology Beijing Beijing 100083 China

Based on the skills of initializing weight distribution, adding an impulse in a neural network and expanding the ideal of plural weights, an artificial neural network model with three connection weights between one and another neural unit was established to predict silicon content of blast furnace hot metal. After the neural network was trained in the off-line state on the basis of a large number of practical data of a commercial blast furnace and making many learning patterns, satisfactory testing and simulating results of the model were obtained.

关键词： blast furnace silicon content neural network Metallurgy

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Alterations on vitamin C synthesis and transportation and egg deposition induced by dietary vitamin C supplementation in Hy-Line Brown layer model

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Animal Nutrition 2021年第4期7卷 973-980页

作者： Yufei Zhu Wei Guo Jianfei Zhao Kailong Qin Jiakun Yan Xinhuo Huang Zhouzheng Ren Xin yang Yanli Liu xiaojun yang College of Animal Science and Technology Northwest A&F UniversityYanglingChina Nano Vitamin Engineering Research Center of Shannxi Province Xi'anChina

In ovo feeding of vitamin C(VC)has positive effects on the growth performance,immune and antioxidant function in poultry,which indicates that increasing VC content in eggs may be of *** study was to investigate the effects of dietary VC supplementation on VC synthesis and transportation and egg *** Exp.1,in order to select a suitable animal model,VC content was detected in different eggs from different layer *** C content was lower in ISA Brown breeder eggs and Hy-Line Brown layer eggs(P<0.05)then in Arbor Acres breeder *** Exp.2,a total of 24 Hy-Line Brown layers(42-week-old)were randomly divided into 3 treatments with 8 replicates and fed a basal diet with VC at 0,200 and 400 mg/***-dependent VC transporter 1 and 2(SVCT1 and SVCT2)expressions were higher in ileum than in duodenum and jejunum(P<0.05).SVCT1 expression was higher but SVCT2 expression was lower in the magnum than in the ovary(P<0.05).L-Gulonolactone oxidase(GLO)and SVCT7 expressions were higher but SVCT2 was lower in the kidney than in the liver(P<0.05).Dietary VC supplementation at 400 mg/kg increased SVCT1 expression in duodenum,ovary and magnum,but decreased GLO and SVCT1 expression in liver(P<0.05).Dietary VC supplementation at 200 and 400 mg/kg increased SVCT2 expression in duodenum,but decreased GLO and SVCT1 expression in kidney and SVCT2 expression in liver(P<0.05).Dietary VC supplementation promoted VC absorption in duodenum and jejunum,but reduced endogenous VC synthesis in liver and *** dietary VC supplementation enhanced VC transportation in ovary and magnum,it did not increase VC deposition in produced eggs.

关键词： Vitamin C Sodium-dependent vitamin C transporter L-Gulonolactone oxidase Albumen Yolk

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Adjusting phosphate feeding regimen according to daily rhythm increases eggshell quality via enhancing medullary bone remodeling in laying hens

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Journal of Animal Science and Biotechnology 2023年第3期14卷 1274-1286页

作者： Jiakun Yan Jiajie Wang Jie Chen Hao Shi Xujie Liao Chong Pan Yanli Liu Xin yang Zhouzheng Ren xiaojun yang College of Animal Science and Technology Northwest A&F UniversityYangling 712100ShaanxiChina

Background Body phosphorus metabolism exhibits a circadian rhythm over the 24-h daily *** egg laying behavior makes laying hens a very special model for investigating phosphorus circadian *** is lack of information about the impact of adjusting phosphate feeding regimen according to daily rhythm on the phosphorus homeostasis and bone remodeling of laying *** and results Two experiments were *** Exp.1,Hy-Line Brown laying hens(n=45)were sampled according the oviposition cycle(at 0,6,12,and 18 h post-oviposition,and at the next oviposition,respectively;n=9 at each time point).Diurnal rhythms of body calcium/phosphorus ingestions and excretions,serum calcium/phosphorus levels,oviduct uterus calcium transporter expressions,and medullary bone(MB)remodeling were *** Exp.2,two diets with different phosphorus levels(0.32%and 0.14%non-phytate phosphorus(NPP),respectively)were alternately presented to the laying ***,four phosphorus feeding regimens in total(each included 6 replicates of 5 hens):(1)fed 0.32%NPP at both 09:00 and 17:00;(2)fed 0.32%NPP at 09:00 and 0.14%NPP at 17:00;(3)fed 0.14%NPP at 09:00 and 0.32%NPP at 17:00;(4)fed 0.14%NPP at both 09:00 and 17:*** a result,the regimen fed 0.14%NPP at 09:00 and 0.32%NPP at 17:00,which was designed to strengthen intrinsic phosphate circadian rhythms according to the findings in Exp.1,enhanced(P<0.05)MB remodeling(indicated by histological images,serum markers and bone mineralization gene expressions),elevated(P<0.05)oviduct uterus calcium transportation(indicated by transient receptor potential vanilloid 6 protein expression),and subsequently increased(P<0.05)eggshell thickness,eggshell strength,egg specific gravity and eggshell index in laying *** These results underscore the importance of manipulating the sequence of daily phosphorus ingestion,instead of simply controlling dietary phosphate concentrations,in modifying the bone remodeling *** phosp

关键词： Body phosphorus rhythm Bone remodeling Eggshell formation Laying hen Phosphorus feeding regimen

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Incubation strategies of the Black-necked Crane(Grus nigricollis) in relation to ambient temperature and time of day

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Avian Research 2017年第3期8卷 147-155页

作者： Lixun Zhang Bei An Meilin Shu Changming Zhao xiaojun yang Yila Suo Yongjun Se Xilite Dabu School of Life Sciences Lanzhou University Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of Sciences National Nature Reserve of Yanchiwan

Background: The behavior of cranes reflects many of their survival strategies, but little has been known of the incubation strategies of cranes, in which both parents share incubation duties, in response to cold temperatures in alpine environments. The lack of information may restrict the effective conservation of the threatened Black-necked Crane(Grus nigricollis), a biparental bird nesting in high elevation ***: We directly observed and used infrared video cameras from 2014 to 2015 to study the incubation behavior and quantitatively measured the frequency and details of egg turning behavior in the Black-necked Crane at the Yanchiwan National Nature Reserve in western Gansu Province, ***: At lower ambient temperatures in the morning, crane parents spent more time on the nest with less recess frequency and prolonged on-bout duration, while at higher temperatures around noon, the parents had more frequent recesses from incubation and shorter periods between nest exchanges. They adjusted the amount of time incubating by varying the recess frequency and the length of on-bout duration. Mean nest attendance and egg turning frequency of females were significantly higher than those of the males. The nest attendance and on-bout duration of females showed a significantly negative relationship with those of males. The two parents responded differently to the change of temperature. Females spent more time on the nest at lower morning temperatures, while males increased their time on the nest at higher temperatures after noon. Higher incubation recess frequency and egg turning frequency were observed at noon, probably because parents spent more time foraging, taking advantage of the lower egg cooling ***: Both Black-necked Crane parents in the alpine environment adjusted their behavior in response to the thermal requirements of eggs and the weather conditions experienced. Our findings demonstrate that parents of this species incubated in diffe

关键词： Time of day Ambient temperature Egg-turning frequency On-bout duration Nest attendance Recess frequency Biparental bird

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Paternal transgenerational nutritional epigenetic effect:A new insight into nutritional manipulation to reduce the use of antibiotics in animal feeding

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Animal Nutrition 2022年第4期11卷 142-151页

作者： Xinyi Li Mengya Wang Shimin Liu Xiaodong Chen Yu Qiao xiaojun yang Junhu Yao Shengru Wu College of Animal Science and Technology Northwest A&F UniversityYanglingShaanxi 712100China Institute of Agriculture University of Western AustraliaCrawleyWA 6009Australia Department of Medicine Karolinska InstitutetSolnaStockholm 17165Sweden Department of Animal Engineering Yangling Vocational and Technical CollegeYanglingShaanxi 712100China

The use of antibiotics in animal feeding has been banned in many countries because of increasing concerns about the development of bacterial resistance to antibiotics and potential issues on food *** for antibiotic substitutes is *** transgenerational epigenetic technology to animal production could be an *** environmental changes can be transferred to memorylike responses in the offspring through epigenetic mechanisms without changing the DNA *** this paper,we reviewed those nutrients and non-nutritional additives that have transgenerational epigenetic effects,including some amino acids,vitamins,and *** paternal transgenerational nutritional epigenetic regulation was particularly focused on mechanism of the substantial contribution of male stud animals to the animal *** illustrated the effects of paternal transgenerational epigenetics on the metabolism and immunity in farming animals and proposed strategies to modulate male breeding livestock or poultry.

关键词： Growth Nutrition Immune Transgenerational epigenetic Antibiotic replacement

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