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Assessment of candidate plant DNA barcodes using the Rutaceae family

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Science China(Life sciences) 2010年第6期53卷 701-708页

作者： LUO Kun1,2,CHEN ShiLin1,CHEN KeLi2,SONG JingYuan1,YAO Hui1,MA XinYe1,ZHU YingJie3.PANG XiaoHui1,YU Hua1,LI XiWen1,4 & LIU Zhen2 1institute of medicinal plant Development,{3. union medical college,chinese academy of medical sciences,Beijing 100193.China 2college of Pharmacy,Hubei University of chinese Medicine,Wuhan 43.061,China 3.chool of Bioscience and Engineering,Southwest Jiaotong University,Chengdu 61003.,China 4Department of Chemistry,Tsinghua University,Beijing 100084,China 1. institute of medicinal plant Development Peking Union Medical College Chinese Academy of Medical Sciences Beijing 100193 China2. College of Pharmacy Hubei University of Chinese Medicine Wuhan 430061 China3. School of Bioscience and Engineering Southwest Jiaotong University Chengdu 610031 China4. Department of Chemistry Tsinghua University Beijing 100084 China

DNA barcoding is a rapidly developing frontier technology that is gaining worldwide ***,seven regions (psbA-trnH,matK,ycf5,rpoC1,rbcL,ITS2,and ITS) with potential for use as DNA barcodes were tested for their ability to identify 3.0 samples of 192 species from 72 genera of the family *** evaluate each barcode’s utility for species authentication,PCR amplification efficiency,genetic divergence,and barcoding gaps were *** found that the ITS2 region exhibited the highest inter-specific divergence,and that this was significantly higher than the intra-specific variation in the "DNA barcoding gap" assessment and Wilcoxon two-sample *** ITS2 locus had the highest identification efficiency among all tested *** a previous study,we found that ITS2 was able to discriminate a wide range of plant taxa,and here we confirmed that ITS2 was also able to discriminate a number of closely related ***,we propose that ITS2 is a promising candidate barcode for plant species identification.

关键词： DNA barcoding ITS2 Rutaceae identification

THE CHEMIOAL STRUCTURE OF EPIMEDOKREANIN A

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chinese Chemical Letters 1994年第4期5卷 311-312页

作者： Wen Kui Kui Fei Gen XIAO(institute of medicinal plant Development, {3. academy ofmedical sciences and peking union medical college, Beijing 100094)Ru Yi ZHANG(School of Pharmaceutical sciences, Beijing medical University,Beijing 100083.

A novel flavone named epimedokoreanin A was isolated from the aerial parts of Epimedium koreanum Nakai .its chemical structure Was determined by spectroscopic methods (MS,1 H NMR,13.C NMR 1H -13.C COSY, 1H-13.long ran...

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Effect of Ginsenoside Rg1 on PP-DG Long Term Potentiation in Rat

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Effect of Ginsenoside Rg1 on PP-DG Long Term Potentiation in...

New Collection of Crude Drugs in chinese Pharmacopoeia 2010 Ⅲ. Kadsurae Caulis

2008 International Conference on Ginseng

作者： Chu Shifeng~1 Wang Xiaoying~2 Wang Yuzhu~3.Chen Ji~1 Chen Naihong~1 Zhang Juntian~1 1.institute of Materia Medica chinese academy of medical sciences peking {3. medical college 2.chinese academy of medical sciences peking {3. medical college institute of medicinal {13. Development 3.Centre for Drug Evaluation State Food and Drug Administration

＜正＞The aim of present paper is to observe the effect of ginsenoside Rg1 on long term potentation and the mechanism of its *** and freely-moving:rats were employed in this *** results showed that Rg1 could improve the synaptic transmission *** receptor antagonisms were used to examine the mechanism of LTP reinforcement induced by Rg1. The results showed that NMDA receptor and metabotropic glutamate receptors play an important role in the induction of LTP induced by Rg1.

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chinese Herbal Medicines 2012年第3期4卷 177-182页

作者： XU Li-jia, MA Pei, LI Li, WANG Wan-ying, XIAO wei, PENG Yong, XIAO Pei-gen Key Laboratory of Bioactive Substances and Resources Utilization of chinese Herbal Medicine, Ministry of Education, institute of {3. plant Development, chinese academy of medical sciences and peking union medical college, Beijing 100193. China Key Laboratory of Bioactive Substances and Resources Utilization of chinese Herbal Medicine Ministry of Education Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193 China

The dried cane of Kadsura interior (Kadsurae Caulis) is recorded in chinese Pharmacopoeia 2010 as Dian Jixueteng for the treatment of rheumatism, irregular menstruation, and deficiency of Qi and blood. In this paper, morphological characteristics, chemical constituents, and pharmacological activities in the cane of K. interior were summarized. Moreover, some suggestions about application and quality control of Kadsurae Caulis were proposed in order to provide a theoretical basis for further scientific use.

关键词： biological activities chemical constituents Kadsurae Caulis Kadsura interior morphological characteristics

Comparison of the Sesquiterpene Ester Profiles of Different Specialized Armillariella mellea Mycelia Using HPLC-DAD Chromatography

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食用菌学报 2006年第4期 44-48页

作者： CHENG Xian-hao 1,2, WANG Chun-lan 1, GUO Shun-xing 1* 1institute of medicinal plant Development, {3. & peking union medical college, Beijing 100094, China 2college of Life Science, Ludong University, Yantai, Shandong 264025, China

Sesquiterpene profiles in methanolic extracts of crustose aerial hyphae, rhizomorphs and fruit bodies of Armillariella mellea grown on semi-solid wort medium, and of fungal mycelium grown in submerged culture on liquid wort medium, were compared using High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD). The sesquiterpene ester components of mycelium grown in submerged culture were distinct from the sesquiterpene esters detected in rhizomorphs, crustose aerial hyphae and fruit bodies. The sesquiterpene ester profile of rhizomorphs was similar to that of crustose aerial hyphae, but was considerably different to the profiles of fungal mycelium grown in submerged culture and fruit bodies.

关键词： Aerial hyphae Armillariella mellea Fruit body HPLC-DAD Rhizomorph Wort medium

Genome-wide and transcriptional analysis of laccases in Ganoderma lucidum

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Genome-wide and transcriptional analysis of laccases in Gano...

High performance liquid chromatographic separation of thirteen drugs collected in chinese Pharmacopoeia 2010(Ch.P2010) on cellulose ramification chiral stationary phase

中药与天然药高峰论坛暨第十二届全国中药和天然药物学术研讨会

作者： Xiaolan Xu~1,Liu He~1,Hongmei Luo~1,Shilin Chen~(*1,2) 1 institute of medicinal plant Development(IMPLAD),{3. & peking union medical college,Beijing 100193.China 2 institute of chinese Materia Medica,academy of chinese medical {13.,Beijing 100700,China

Ganoderma lucidum,a wood-rotting fungi,has been used as medicinal mushroom for treating many diseases such as cancer,tumor,hepatitis,chronic bronchitis,gastritis and so *** addition,*** is a kind of white-rot basidiomycetes that could cause ***(EC 1.***.3.2)is a multi-copper blue oxidase that distribute widely in plants and *** research indicate that they have multiple laccase isozymes and some have been purified.A new genome-wide analysis of laccases was performed in *** the present study,a detailed analysis of thirteen laccases was *** them,there are three laccases were previous reported(lacc1,lacc2, lacc3..We investigated the laccases gene expression in different period of growth including the mycelia,primordia,and fruiting bodies in *** by using reverse transcription(RT)-PCR. Lastly,homologous laccase sequences were retrieved from NCBI and JGI web *** sequence alignments and phylogenetic trees were *** comparison was explored to understand the functional diversities in basidiomycetes.

关键词： laccases genome-wide gene expression Ganoderma lucidum

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Journal of Pharmaceutical Analysis 2012年第1期2卷 48-55页

作者： Ying Zhoua,b, Chao Maa, Yan Wanga, Qi-Ming Zhangb, Yi-Ying Zhanga, Jie Fua, Hong Gaoc, Li-Xun Zhaoc aState Key Laboratory of Bioactive Substances and Functions of Natural Medicines, institute of Materia Medica, chinese academy of medical sciences & peking {3. medical college, Beijing 100050, China bNational institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China cinstitute of medicinal Biotechnology, chinese academy of medical sciences & peking {3. medical college, Beijing 100050, China State Key Laboratory of Bioactive Substances and Functions of Natural Medicines Institute of Materia Medica Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100050 China. National institute for the Control of Pharmaceutical and Biological Products Beijing 100050 China. institute of medicinal Biotechnology Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100050 China.

The enantiomers separation of thirteen drugs collected in Ch.P2010 was performed on chiral stationary phase of cellulose ramification (chiralpak OD and chiralpak OJ) by high performance liquid chromatographic (HPLC) methods, which included ibuprofen (C1), ketoprofen (C2), nitrendipine (C3., nimodipine (C4), felodipine (C5), omeprazole (C6), praziquantel (C7), propranolol hydrochloride (C8), atenolol (C9), sulpiride (C10), clenbuterol hydrochloride (C11), verapamil hydrochloride (C12), and chlorphenamine maleate (C13.. The mobile phase consisted of isopropanol and n-hexane. The detection wavelength was set at 254 nm and the fiow rate was 0.7 mL/min. The enantiomers separation of these thirteen racemates on chiralpak OD column and chiralpak OJ column was studied, while the effects of proportion of organic additives, alcohol displacer and temperature on the separation were studied. And the mechanism of some of racemates was discussed. The results indicated that thirteen chiral drugs could be separated onchiral stationary phase of cellulose ramification in normal phase chromatographic system. The chromatographic retention and resolution of enantiomers could be adjusted by factors including column temperature and the concentration of alcohol displacer and organic alkaline modifier in mobile phase. It was shown that the resolution was improved with reducing concentration of alcohol displacer. When concentration of organic alkaline modifier was 0.2% (v/v), the resolution and the peak shape were fairly good. Most racemates mentioned above had better resolution at column temperature of 25 1C. When racemates were separated, the temperature should be kept so as to obtain stable separation results.

关键词： HPLC Chiral stationary phase Optical enantiomers Cellulose ramification

Drug screening for influenza neuraminidase inhibitors

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Science China(Life sciences) 2005年第1期48卷 1-5页

作者： LIU Ailin, CAO Hongpeng & DU Guanhua institute of Materia Medica, chinese academy of medical sciences and peking {3. medical college, Beijing 100050, China institute of medicinal Biotechnology, chinese academy of medical sciences and peking {3. medical college, Beijing 100050, China institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050 Chin

Neuraminidase (NA) is one of the most important targets to screen the drugs of anti-influenza virus A and B. After virtual screening approaches were applied to a compound database which possesses more than 10000 compound structures, 160 compounds were selected for bioactivity assay, then a High Throughput Screening (HTS) model established for influenza virus NA inhibitors was applied to detect these compounds. Finally, three compounds among them displayed higher inhibitory activities, the range of their IC5o was from 0.1 μmol/L to 3.μmol/L. Their structural scaffolds are novel and different from those of NA inhibitors approved for influenza treatment, and will be useful for the design and research of new NA inhibitors. The result indicated that the combination of virtual screening with HTS was very significant to drug screening and drug discovery.

关键词： neuraminidase (NA), inhibitor, high throughput screening (HTS), influenza virus, virtual screening.

DAPHNIPHYLLUM ALKALOIDS

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DAPHNIPHYLLUM ALKALOIDS