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Identification of Molecular Markers for a Aphid Resistance Gene in Sorghum and Selective Efficiency Using These Markers

第四届智能化农业信息技术国际学术会议

作者： tieying xue yang zhang beijing vocational college of agriculture Post Code: 102442 beijing China

＜正＞The key of Chinese agricultural information digitization （AID） is to apply computers to rural *** a developing country of agriculture,a vast group of technically talented people are badly needed,who are welcomed by peasants and are willing to stay in country *** are the features of New Rural Area （NRA） ? That means information digitization enters villages,touches farmers’ life and is applied to agricultural ***, the quantity and quality of higher vocational education （HVE） for computers are pretty essential in Chinese agricultural information digitization process.

关键词： Agricultural information digitization(AID) New Rural Area(NRA) higher vocational education(HVE) agricultural vocational college(AVC) talent standards training curriculum computer case study

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Journal of Integrative agriculture 2012年第7期11卷 1086-1092页

作者： CHANG Jin-hua CUI Jiang-hui xue Wei zhang Qing-wen college of agriculture and Biotechnology China Agricultural University Beijing 100096 P.R.China college of agriculture Agricultural University ofHebei Baoding 071001 P.R.China Student Employment Guidance Center Baoding Vocational and Technical College Baoding 071001 P.R.China

In this study, an F2 segregated population obtained by hybridization between the aphid-sensitive sorghum strain Qiansan and aphid-resistant cultivar Henong 16 was used to establish an aphid-resistant pool and an aphid-sensitive pool. 192 pairs of AFLP （amplified fragment length polymorphism） marker primers were screened in these pools using BSA （bulked segregant analysis）. Three pairs of EcoR I-CTG/Mse I-CCT, EcoR I-CTG/Mse I-CAT, and EcoR I-AGT/Mse I-CCC showed linkage with aphis resistance. EcoR I-CTG/Mse I-CCT-475, EcoR I-CTG/Mse I-CAT-390, and EcoR I-AGT/Mse I-CCC- 350 （E42/M52-350） were mapped within 6, 10, and 13 cM distances with the aphid-resistant gene by using Mapmaker 3.0 software. The bands amplified by EcoR I-CTG/Mse I-CCT-475 and EcoR I-CTG/Mse I-CAT-390 were extracted, cloned, and sequenced. Specific primers of SCAR （sequence characterized amplified regions） were then designed from these bands. A specific band of 300 bp was amplified by a pair of SCAR primers designed based on the sequence obtained from the EcoR I-CTG/Mse I-CAT-390 marker. The SCAR marker was named SCAS0. The marker was used to detect the F2, BC1, and F2：3 populations. The selective efficiency was 86.8, 91.1, and 86.3% in the BC1, F2, and F2：3 populations, respectively. The average selective efficiency was 88.2%.

关键词： sorghum bicolor aphid resistance gene molecular marker SCAR molecular assistant selection

Role of the rhizosphere of a flooding-tolerant herb in promoting mercury methylation in water-level fluctuation zones

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Journal of Environmental Sciences 2022年第9期34卷 139-151页

作者： Juan Wang Yuping Xiang Xiaosong Tian Cheng zhang Guiqing Gong Jinping xue Tao Jiang Dingyong Wang Yongmin Wang Interdisciplinary Research Center for agriculture Green Development in yangtze River Basin College of Resources and EnvironmentSouthwest UniversityChongqing 400715China Laboratory of Environmental Nanotechnology&Health Effect Chinese Academy of SciencesResearch Center for Eco-Environmental SciencesBeijing 100085China college of Resources and Safety Chongqing Vocational Institute of EngineeringChongqing 402260China Institut des Sciences Analytiques et des Physico-Chimie pour l’Environnement et les Materiaux(IPREM) UMR 5254CNRSUniversitede Pau et des Pays de l’AdourPau 64000France

The water-level fluctuation zone(WLFZ) has been considered as a hotspot for mercury(Hg) methylation. Flooding-tolerant herbs are gradually acclimated to this water-land ecotone, tending to form substantial root systems for improving erosion resistance. Accompanying rhizosphere microzone plays crucial but unclear roles in methylmercury(Me Hg) formation in the WLFZ. Thus, we conducted this study in the WLFZ of the Three Gorges Reservoir, to explore effects of the rhizosphere of a dominant flooding-tolerant herb(bermudagrass) on Me Hg production. The elevated Hg and Me Hg in rhizosphere soils suggest that the rhizosphere environment provides favorable conditions for Hg accumulation and methylation. The increased bioavailable Hg and microbial activity in the rhizosphere probably serve as important factors driving Me Hg formation in the presence of bermudagrass. Simultaneously, the rhizosphere environments changed the richness, diversity, and distribution of hgc A-containing microorganisms. Here, a typical ironreducing bacterium( Geobacteraceae) has been screened, however, the majority of hgc A genes detected in rhizosphere, near-, and non-rhizosphere soils of the WLFZ were unclassified. Collectively, these results provide new insights into the elevated Me Hg production as related to microbial processes in the rhizosphere of perennial herbs in the WLFZ, with general implications for Hg cycling in other ecosystems with water-level fluctuations.

关键词： Three Gorges Reservoir Water-level fluctuation zone Rhizosphere soil Methylmercury Mercury methylator

基于生态位模型的南、北五味子潜在适生区预测及其影响因子研究

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湖北农业科学 2024年第1期63卷 116-121页

作者：张迪薛明珂刘明尚晓峰张旭彤刘金娜杨凌职业技术学院陕西杨凌712100 中国农业科学院农业环境与可持续发展研究所北京100081

基于184条南五味子(Schisandra sphenanthera)地理分布记录、129条北五味子(Schisandra chinensis)地理分布记录以及23个环境因子数据,采用MaxEnt生态位模型预测南、北五味子潜在适宜分布区,评估了制约其地理分布的主要环境因子。结果表... 详细信息

基于184条南五味子(Schisandra sphenanthera)地理分布记录、129条北五味子(Schisandra chinensis)地理分布记录以及23个环境因子数据,采用MaxEnt生态位模型预测南、北五味子潜在适宜分布区,评估了制约其地理分布的主要环境因子。结果表明,北五味子和南五味子MaxEnt模型训练集和验证集曲线下面积(AUC)均大于0.90,建立的模型预测结果准确。北五味子最佳生境主要分布在辽宁省和吉林省的长白山区域以及北京市;南五味子的最佳生境主要分布在秦巴山区内,以及湖南省武陵山脉,郴州市、株洲市和衡阳市,浙江省金华市等地区。限制北五味子生长的重要环境因子变量分别是最湿月份降水量、最热月份最高温度、土壤酸碱度、最暖季平均降水量、最冷季平均降水量和昼夜温差月均值;限制南五味子分布的环境因子变量分别是最冷月份最低温温度、年平均降水量、温度变化方差和降水量变化方差。南、北五味子的潜在适生区呈区域化分布。

关键词：南五味子(Schisandra sphenanthera) 北五味子(Schisandra chinensis) MaxEnt模型潜在适生区环境因子

Present Situation and Prospect of Grub Control in Sweet Potato Field

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Plant Diseases and Pests 2015年第2期6卷 1-4,15页

作者： Yan Hui xue Cheng Li Qiang zhang Yungang Xuzhou Sweet Potato Research Center/Key Laboratory of Sweet Potato Biology and Genetic Breeding Ministry of Agriculture/Sweet Potato Research InstituteChinese Academy of Agricultural Sciences Xuzhou vocational college of Bioengineering

Grub is one of the major pests affecting yield and quality of sweet potato. Although traditional chemical pesticides have quick effect, the disadvantages of high toxicity, strong pest resistance, and heavy pollution hinder the development of modern ecological agriculture, while physical means such as light trapping, sex pheromone method and biological control methods are more safe and lasting compared with chemical means. To provide reference and guidance for grub control and sweet potato production, the control methods against sweet potato grubs are comprehensively explored, and strategy and prospects of using biological technology to control pests are analyzed.

关键词： Sweet potato Grub Biological control Present situation Prospect

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国内典型创业教育模式对河南高校的启示

北京农业职业学院学报 2017年第2期31卷 84-90页

作者：张学艳郑州工商学院河南郑州451400

2015年的政府工作报告中,明确把"大众创业、万众创新"作为推动中国经济发展的"双引擎"之一。高校是创新创业教育开展的主体,培养出具有创新意识和创新思维的创新创业型人才,对解决当前严峻的就业形势,建设创新型国家具有重要的意义。本... 详细信息

2015年的政府工作报告中,明确把"大众创业、万众创新"作为推动中国经济发展的"双引擎"之一。高校是创新创业教育开展的主体,培养出具有创新意识和创新思维的创新创业型人才,对解决当前严峻的就业形势,建设创新型国家具有重要的意义。本文通过对我国典型的创业教育模式进行分析,找出河南省高校创业教育存在的问题,总结国内典型创业教育模式对河南高校的启示。

关键词：创业教育教育模式大学生创业

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渝东南地区经济社会发展对比分析及思路探索

北京农业职业学院学报 2016年第1期30卷 49-55页

作者：王傲雪张涛重庆工商大学重庆400067

渝东南既是我国连片特困地区,又是旅游资源富集区,现为重庆市生态保护发展区。区内具有旅游资源优势,近年来逐步改善的交通条件和良好的生态环境为区内经济社会发展带来重要机遇,但对比分析整个渝东南地区目前的经济社会发展现状,得出... 详细信息

渝东南既是我国连片特困地区,又是旅游资源富集区,现为重庆市生态保护发展区。区内具有旅游资源优势,近年来逐步改善的交通条件和良好的生态环境为区内经济社会发展带来重要机遇,但对比分析整个渝东南地区目前的经济社会发展现状,得出该地区发展水平参差不齐,经济总量小、对外开放程度低、产业结构不合理、城市化率偏低、社会事业发展严重滞后、扶贫进程缓慢的结论。分区县来看,除了武隆地区完成了产业结构的转型,其他地区仍依靠重工业经济支撑,并未发挥出旅游业带动第三产业发展的优势。基于此,从扶贫旅游开发、产业结构调整、人才培育三个层面提出发展思路,以促进渝东南民族地区更好更快地发展。

关键词：渝东南民族地区产业结构旅游资源脱贫致富

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方向与路径:新农村背景下的乡镇企业发展

北京农业职业学院学报 2008年第2期22卷 45-49页

作者：张玉军沈学苏州大学商学院江苏苏州215021

工业反哺农业是和谐新农村建设中的要求,乡镇企业作为与农村联系最为紧密的企业形式注定要重任在肩。目前在乡镇企业发展过程中,存在着规模小、布局分散、产业结构不合理、环境污染等一系列问题。乡镇企业集群是新农村背景下的乡镇企业... 详细信息

工业反哺农业是和谐新农村建设中的要求,乡镇企业作为与农村联系最为紧密的企业形式注定要重任在肩。目前在乡镇企业发展过程中,存在着规模小、布局分散、产业结构不合理、环境污染等一系列问题。乡镇企业集群是新农村背景下的乡镇企业发展的理想路径,应从产业集群发展的环境、企业集聚的机制、特色主导产业的发展等方面推动乡镇企业集群化发展。

关键词：乡镇企业新农村集群

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阿拉伯国家渔业发展概况及启示

渔业信息与战略 2021年第1期36卷 54-63页

作者：薛英杰张红智对外经济贸易大学北京100029 山东外贸职业学院山东青岛266100

利用联合国粮食及农业组织(Food and agriculture Organization of the United Nations,FAO)FishStat J数据库,分析了自1950年以来,阿拉伯国家的渔业生产和构成情况。根据渔业资源禀赋、产业政策和发展环境将其分为渔业生产主产国、潜... 详细信息

利用联合国粮食及农业组织(Food and agriculture Organization of the United Nations,FAO)FishStat J数据库,分析了自1950年以来,阿拉伯国家的渔业生产和构成情况。根据渔业资源禀赋、产业政策和发展环境将其分为渔业生产主产国、潜力型国家、待开发型国家和欠缺型国家,并从资源禀赋、产业政策和发展环境3个方面,重点分析了影响阿拉伯国家渔业主产国及其他阿拉伯国家渔业发展的有利条件和制约因素。最后,结合现阶段中国提出的“一带一路”倡议,提出了中国-阿拉伯国家“1+2+3”合作框架下渔业合作启示与思考。

关键词：阿拉伯国家渔业主产国启示