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Enhancement of intranasalmucosal immunization of mucosal vaccines by ultrasonic treatment

Bio-Design and Manufacturing 2023年第4期6卷 405-422页

作者： Haowei Xu yang liao Mankovskaya Svetlana Deguang yang Huaibin Wan Zonghua Liu Department of Biomedical Engineering Jinan UniversityGuangzhou 510632China Department of Laboratory Medicine General Hospital of Southern Theatre Command of PLAGuangzhou 510010China Institute of Physiology of NAS of Belarus MinskBelarus Department of Cardiology Heyuan Shenhe People’s HospitalThe Fifth Affiliated HospitalJinan UniversityHeyuan 517475China

The pathogens of most infectious diseases invade the host through mucosal sites,and immunization with mucosal vaccines is the best means of combating these infectious *** delivery and nasal delivery are the most common methods of mucosal ***,the delivery process is inefficient,and mucosal vaccination is ineffective because the vaccine formulation is easily and rapidly removed and has difficulty in crossing the mucosal *** this paper,we investigated whether the mucosal immune response could be enhanced by ultrasound facilitation of nasal mucosal delivery of vaccine *** this purpose,we used manganese dioxide(MnO2)as the vaccine carrier/adjuvant,coated with chitosan oligosaccharide(COS)to enhance mucosal adsorption,and further physically adsorbed model antigen ovalbumin(OVA)to construct a nanoparticulate vaccine formulation MnO2@COS@*** treatment was found to promote antigen delivery and recruitment of dendritic cells(DCs)and macrophages as well as T-cell infiltration in nasal mucosal tissues through nasal mucosal immunization *** ultrasound assistance,MnO2@COS@OVA particles promoted the maturation of DCs in vitro and in vivo and promoted the production of effector memory T cells in vivo and cytokine secretion by splenocytes in *** particular,ultrasound treatment significantly increased the levels of secretory IgA antibodies in the nasal mucosa and genital tract mucosa of experimental *** addition,the experimental data showed that the MnO2@COS@OVA particles had good biocompatibility and caused no significant damage to the nasal mucosal and vital organ *** data suggest that ultrasound treatment can promote the induction of efficient immune responses to mucosal vaccines and provide new ideas for the opening and clinical translation of mucosal vaccines.

关键词： Mucosal vaccine Ultrasound treatment MnO2 Nasal delivery

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无患子雄性不育品种‘琦蕊’不同发育时期雄花转录组分析

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生物技术通报 2024年第7期40卷 197-206页

作者：廖杨梅赵国春翁学煌贾黎明陈仲北京林业大学林学院林木资源高效生产全国重点实验室省部共建森林培育与保护教育部重点实验室国家能源非粮生物质原料研发中心北京100083 福建源华林业生物科技有限公司三明354500

【目的】探究无患子(Sapindus mukorossi Gaertn.)雄性不育品种‘琦蕊’雄花不同发育时期的细胞学特征及差异表达基因,为深入解析无患子雄性不育发生的分子机制提供理论依据。【方法】在观察雄花花药不同发育过程的细胞学特点基础上,进... 详细信息

【目的】探究无患子(Sapindus mukorossi Gaertn.)雄性不育品种‘琦蕊’雄花不同发育时期的细胞学特征及差异表达基因,为深入解析无患子雄性不育发生的分子机制提供理论依据。【方法】在观察雄花花药不同发育过程的细胞学特点基础上,进一步利用RNA-Seq技术分别对小孢子母细胞时期(T1)、四分体时期(T2)、单核小孢子时期(T3)的雄花进行转录组比较分析,筛选关键差异表达基因。【结果】‘琦蕊’绒毡层和内层细胞的持续膨大与增殖使得药室结构混乱,药室空间不足,最终导致无可育花粉产生。内源激素测定发现,茉莉酸水平在‘琦蕊’雄花发育过程中呈下降趋势。3个时期的转录组分析共筛选到2990个差异表达基因(differentially expressed genes,DEGs),其中T2_vs_T1中722个(516个上调,206个下调),T3_vs_T2中1741个(765个上调,976个下调)。GO和KEGG分析表明,这些DEGs主要富集在代谢过程、细胞分裂、花粉壁合成、激素信号转导等方面,共鉴定到32个花粉发育相关DEGs、27个激素合成及信号转导DEGs。随机选取9个DEGs进行RT-qPCR分析,结果与RNA-Seq数据趋势一致,证明转录组数据的准确性。【结论】绒毡层细胞持续增殖和内层细胞延迟退化不断挤压小孢子是导致无患子‘琦蕊’雄性不育发生的主要原因,物质代谢、细胞分裂、激素水平、花粉发育等相关基因在雄性不育发生过程中发挥重要作用。

关键词：无患子雄性不育转录组差异表达基因绒毡层细胞分裂茉莉酸

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人口老龄化背景下非农收入对农户清洁能源使用的调节效应研究

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中国物价 2024年第4期 124-128页

作者：廖阳覃芳李迎峰西安建筑科技大学管理学院

基于中国家庭追踪调查2018年数据,本文从微观角度考察了家庭人口老龄化对农户清洁能源使用的影响,并探究了不同非农收入类型对老龄化家庭清洁能源使用的调节作用。结果表明:不同年龄结构的家庭类型在能源使用上存在差异,家庭人口老化特... 详细信息

基于中国家庭追踪调查2018年数据,本文从微观角度考察了家庭人口老龄化对农户清洁能源使用的影响,并探究了不同非农收入类型对老龄化家庭清洁能源使用的调节作用。结果表明:不同年龄结构的家庭类型在能源使用上存在差异,家庭人口老化特征显著降低了农户清洁能源使用的概率。非农收入对此具有显著的调节作用,其中社会保障缓解了户主年龄老化对农户清洁能源使用的阻碍作用,而政府补助和非农就业收入则增强了家庭人口结构老化对农户清洁能源使用的不利影响。据此,提出促进农村能源转型的对策建议。

关键词：老龄化家庭清洁能源使用非农收入调节效应

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NOVEL AMPHIPHILIC FLUORESCENT GRAFT COPOLYMER: SYNTHESIS, CHARACTERIZATION AND ENCAPSULATION OF A HYDROPHOBIC AGENT

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Chinese Journal of Polymer Science 2006年第3期24卷 315-321页

作者： Zhao-qiang Wu Shu yang Wen-yan liao 孟令芝 College of Chemistry and Molecular Sciences Wuhan University Wuhan 430072 China

Novel amphiphilic fluorescent graft copolymer （PVP-PyAHy） was successfully synthesized by the free radical copolymerization of hydrophobic monomer N-（1-pyrenebutyryl）-N＇-acryloyl hydrazide （PyAHy） with hydrophilic precursor polymers of vinyl-functionalized poly（N-vinylpyrrolidone）（PVP） in DMF. The copolymer is amphiphilic and has intrinsic fluorescence. FT-IR, ^1H-NMR, TEM, gel permeation chromatography-multi-angle laser light scattering, UV-Vis spectroscopy and fluorescence spectroscopy were used to characterize this copolymer. The TEM observation shows that the copolymer PVP-PyAHy forms micelles in aqueous solution. Results of fluorometric measurements illustrate that the critical micelle concentration （CMC） value of PVP-PyAHy in aqueous solution is about 0.90 mg/mL. To examine the encapsulation ability of the copolymer in aqueous media, methyl yellow was employed as a model hydrophobic agent. The loading level of the polymer to methyl yellow is 8.8 mg/g. The cytotoxicity assays for Madin Darby Canine Kidney （MDCK） cells shows good biocompatibility of PVP-PyAHy in vitro. These results suggest the potential of this copolymer PVP-PyAHy as drugs delivery carrier and fluorescent tracer.

关键词： Amphiphilic copolymer Fluorescence Poly(N-vinylpyrrolidone).

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In situ Growing PtCo Bimetallic Catalyst on Plant Tannin-grafted Collagen Fiber for Catalytic Hydrogenation of Cinnamaldehyde with Desirable Performance

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Chemical Research in Chinese Universities 2018年第2期34卷 285-289页

作者： liao Ziyi LAN yang WANG Kai LEI Miao liao yang MAO Hui MA Jun ZHAO Shilin Department of Scienttfic Research Sichuan Normal University Chengdu 610068 P. R. China College of Chemistry and Materials Science Sichuan Normal University Chengdu 610068 P. R. China Engineering Research Center for the Development of Farmland Ecosystem Service Functions Sichuan Province Institutions of Higher Education Chengdu 610068 P. R. China

PtCox bimetallic nanoparticles（NPs） supported on tannin-grafted collagen fiber（CF-BT） have been pre- pared via a novel synthetic strategy, and applied for catalytic hydrogenation of cinnamaldehyde（CAL）, a typic unsa- turated aldehyde. The catalysts were systematically artd specifically characterized by means of XRD, XPS, TEM-EDX and SEM to clarify the structure-property correlation. It was found that the PtCox/CF-BT catalysts exhi- bited significantly enhanced catalytic activity and desirable stability in catalytic hydrogenation of CAL, which is ascribed to the synergistic interaction between bimetallic components, the effective dispersion, the anchoring role of CF-BT matrix on bimetallic NPs, as well as the lower mass transfer resistance of the matrix.

关键词： Heterogeneous PtCox bimetallic catalyst Tannin-grafted collagen fiber Cinnamaldehyde Liquid-phase hydrogenation

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Monitoring variations of inland lakes in the arid region of Central Asia

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Frontiers of Earth Science 2012年第2期6卷 147-156页

作者： Jie BAI Xi CHEN liao yang Hui FANG State Key Laboratory of Desert and Oasis Ecology Xinjiang Institute of Ecology and GeographyChinese Academy of SciencesUrumqi 830011China

Inland lakes are the major surface water resource in the arid regions of Central Asia. Therefore, the surface area changes in inland lakes have been a sensitive indicator of climate changes and human activ- ities, and have often been the focus of ecological and environmental research. This study aimed to monitor the changes in surface area of nine major lakes over a 32-year period. The water body was extracted from MSS images from the mid-1970s, TM images from the early 1990s, ETM ＋ images in the late 1990s, and TM images in 2007. The results indicated that the total surface area of these nine lakes had decreased over time to 50.38% of the area, from 91402.06km^2 in 1975 to 46049.23 km^2 in 2007. As the surface area of lakes in the western part of Central Asia was larger than that in the eastern part, the shrinking trend of lake area was more significant in the west than in the east. There was a varied reduction of closed lakes in flat regions. The most substantial decrease was in the surface area of closed lakes in flat regions. Most significantly, the area of the Aral Sea was reduced by 75.7% from its original area in 1975. The area of alpine lakes remained relatively stable; the change in surface area was less than 0.7% during the period 1975-2007. The area change in opened lakes with outlets was notably different from the other two types. The area of Zaysan had increased sharply by 5.85%, and that of Bosten had decreased by 9.1%. Sasykkol had hardly any changes in this period. Due to global climate wanning, vapor transfer to the south via westerly winds had been blocked, resulting in a decrease of much-needed precipitation in the western parts of Turkmenistan, Uzbekistan, and Kazakhstan between 1970 and 2000. The decrease in precipitation and the increase in water consumption for agricultural irrigation resulted in the decrease of fiver runoff. Consequently, thearea of inland lakes in Central Asia shrank over the past 32 years.

关键词： Central Asia inland lake area change climatechange

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Breeding ecology of ground tits in northeastern Tibetan plateau, with special reference to cooperative breeding system

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Current Zoology 2011年第6期57卷 751-757页

作者： Xin LU Ran HUO yang LI Wenbo liao Chen WANG Department of Zoology College of Life Sciences Wuhan University Wuhan 430072 China

Data on breeding ecology of a color-band marked population of the ground tit Parus humilis were collected in north Qinghai on the Tibetan plateau, during 2008 and 2009. In spring the birds excavated 0.8-3.2 m long nesting burrows under the ground. First-egg laying occurred between late April and late June during which a pair produced one brood. Incubation was done by female alone for 15-16 days and nestling-feeding by both sexes and helpers in any for 23-25 days. Average brood size at fledging was 5.8 （＋ 1.4 SD, 3-8） and all the 27 observed nesting attempts fledged at least one young. At the population level, brood sex ratio did not differ from 1：1. The birds are a territory-living resident, with annual resight rates being 48% （22 of 46） in adult breeders and 10% （7 of 67） in yearlings. Pairs were socially monogamous, of which 23% （9 of 40） contained one and some- times two male helpers, most likely being philopatric sons of the breeders. The formation of cooperative groups is similar to the population in central Tibet but differs from that in south Guansu where breeding ground tits exhibit a high level of annual turnover

关键词： Burrow-nesting Cooperative breeding Life history Parus humilis Tibetan plateau

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PCBP1 modulates the innate immune response by facilitating the binding of cGAS to DNA

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Cellular & Molecular Immunology 2021年第10期18卷 2334-2343页

作者： Chen-yang liao Cao-Qi Lei Hong-Bing Shu Department of Infectious Diseases Frontier Science Center for Immunology and MetabolismMedical Research InstituteZhongnan Hospital of Wuhan UniversityWuhan University430071 WuhanChina

Cyclic GMP-AMP synthase(cGAS)is a key sensor critical for the recognition of DNA in the cytosol and catalyzes the synthesis of the second messenger cyclic GMP-AMP(cGAMP),which binds to the adapter protein MITA(also known as STING,MPYS,and ERIS)to initiate the innate immune *** the binding of DNA to and the activation of cGAS are regulated remains poorly *** a biochemical purification approach,we identified poly(rC)-binding protein 1(PCBP1)as a cGAS-associated ***1 was recruited to cGAS in a viral infection-dependent ***1 directly bound to DNA and enhanced cGAS binding to its ligands,which was important for cGAS ***,PCBP1 deficiency inhibited cytosolic DNA-and DNA virus-triggered transcription of downstream effector *** findings suggest that PCBP1 plays an important role in the cGAS-mediated innate immune response to DNA virus infection by promoting the binding of cGAS to viral DNA.

关键词： cGAS PCBP1 DNA virus Innate Immunity

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Gold and antimony metallogenic relations and ore-forming process of Qinglong Sb(Au) deposit in Youjiang basin, SW China: Sulfide trace elements and sulfur isotopes

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Geoscience Frontiers 2021年第2期12卷 605-623页

作者： Jun Chen Zhi-Long Huang Rui-Dong yang Li-Juan Du Ming-yang liao State Key Laboratory of Ore Deposit Geochemistry Institute of GeochemistryChinese Academy of SciencesGuiyang550002China College of Resources and Environmental Engineering Guizhou UniversityGuiyang550025China Key Laboratory of Karst Georesources and Environment Ministry of EducationGuizhou UniversityGuiyang550025China School of Mining Engineering Guizhou Institute of TechnologyGuiyang550003China

In the northwestern margin of the Youjiang basin(NWYB)in SW China,many Carlin-like gold deposits are highly antimony(Sb)-rich,and many vein-type Sb deposits contain much *** deposits have similar ages,host rocks,ore-forming temperatures,ore-related alterations and ore mineral assemblages,but the Au and Sb metallogenic relations and their ore-forming process remain *** we investigate the large Qinglong Sb deposit in the NWYB,which has extensive sub-economic Au mineralization,and present a new metallogenic model based on in-situ trace elements(EPMA and LA-ICP-MS)and sulfur isotopes(NanoSIMS and fs-LA-MC-ICPMS)of the ore *** Qinglong,economic Sb ores contain coarse-grained stibnite,jasperoid quartz and fluorite,whilst the sub-economic Au–Sb ores comprise dominantly veined quartz,arsenian pyrite and fine-grained *** generations of ore-related pyrite(Py1,Py2 and Py3)and two generations of stibnite(Stb1 and Stb2)are identified based on their texture,chemistry,and sulfur *** pre-ore Py1 is characterized by the lower ore element(Au,As,Sb,Cu and Ag)contents(mostly below the LA-ICP-MS detection limit)and Co/Ni ratios(average 0.31)than the ore-stage pyrites(Py2 and Py3),implying a sedimentary/diagenetic *** Py2 and Py3 have elevated ore element abundance(maximum As=6500 ppm,Au=22 ppm,Sb=6300 ppm,Cu=951 ppm,Ag=77 ppm)and Co/Ni ratios(average 1.84),and have positive As ***–Sb–Cu–Ag ***-ore Stb1 has lower As(0.12–0.30 wt.%)than late-ore Stb2(0.91–1.20 wt.%).These features show that the progressive As enrichment in ore sulfides is accompanied by increasing Au,Sb,Cu and Ag with the hydrothermal evolution,thereby making As a good proxy for ***-rich,As-poor and As-free zones are identified via NanoSIMS mapping of the Au-bearing *** As-rich zones in the Qinglong Au-bearing pyrites(Py2 and Py3)and ore stibnites(Stb1 and Stb2)have narrowδ^(34)SH_(2)S ranges(-8.9‰to +4.1‰,average-3.1‰)and-2.9‰to +6.9‰,average +

关键词： Pyrite Stibnite LA-ICP-MS and NanoSIMS Sulfur isotopes Au and Sb deposits Youjiang basin

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学科前沿成果为载体的国家一流课程生物化学教学与育人有机融合的探索与实践

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微生物学杂志 2024年第4期44卷 119-128页

作者：闫荣玲廖阳李常健覃佐东曾二青湖南科技学院化学与生物工程学院湖南永州425199

局限教材有限内容与课堂有限时空的传统教学,不能很好地契合新时代金课标准以及高素质人才培养的需要。借助信息技术在课程教学不同环节融入学科前沿研究成果是优化教学内容、突破时空界限、提高学生素养、促进教学与育人有机融合的有... 详细信息

局限教材有限内容与课堂有限时空的传统教学,不能很好地契合新时代金课标准以及高素质人才培养的需要。借助信息技术在课程教学不同环节融入学科前沿研究成果是优化教学内容、突破时空界限、提高学生素养、促进教学与育人有机融合的有效途径。国家一流本科课程“生物化学”教学团队借助学银在线、微信共享、虚拟仿真等信息化平台,把学科前沿成果巧妙融入课中教学活动或课前、课后的自主任务中,创新了课程教学模式与方法,提升了学生学习兴趣,夯实了学生综合能力,同时熏陶了学生创新思维、求真品质、人文素养、家国情怀,很好地实现了课程教学创新与思政育人的有机融合。

关键词：生物化学前沿成果教学模式教学效果思政育人信息技术

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