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Alternative splicing of inner-ear-expressed genes

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Frontiers of Medicine 2016年第3期10卷 250-257页

作者： yanfei wang Yueyue Liu Hongyun Nie Xin Ma Zhigang Xu Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology School of Life Sciences Shandong University Jinan 250100 China School of Control Science and Engineering Shandong University Jinan 250061 China

Alternative splicing plays a fundamental role in the development and physiological function of the inner ear. Inner-ear-specific gene splicing is necessary to establish the identity and maintain the function of the inner ear. For example, exon 68 of Cadherin 23 （Cdh23） gene is subject to inner-ear-specific alternative splicing, and as a result, Cdh23（＋ 68） is only expressed in inner ear hair cells. Alternative splicing along the tonotopic axis of the cochlea contributes to frequency tuning, particularly in lower vertebrates, such as chickens and turtles. Differential splicing of Kcnmal, which encodes for the a subunit of the Ca2＋-activated K~ channel （BK channel）, has been suggested to affect the channel gating properties and is important for frequency tuning. Consequently, deficits in alternative splicing have been shown to cause hearing loss, as we can observe in Bronx Waltzer Cov） mice and Sfswap mutant mice. Despite the advances in this field, the regulation of alternative splicing in the inner ear remains elusive. Further investigation is also needed to clarify the mechanism of hearing loss caused by alternative splicing deficits.

关键词： alternative splicing inner ear hearing loss hair cells

A super-resolution algorithm for synthetic aperture radar based on modified spatially variant apodization

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Science China(Physics,Mechanics & Astronomy) 2011年第2期54卷 355-364页

作者： NI Chong wang yanfei XU XiangHui ZHOU ChangYi CUI PengFei Institute of Electronics Chinese Academy of SciencesBeijing 100190 China Graduate University of the Chinese Academy of Sciences Beijing 100190 China

The existing spatially variant apodizations(SVAs) either cannot depress the sidelobes effectively or reduce the energy of the *** improve this,a modified SVA(MSVA) is put forward in this paper,which expands the traditional filter from 3-taps to 5-taps and sets relevant parameters according to different sampling rates to get the excellent result that satisfies constrained optimization theory.A modified super-SVA is also presented,which compares the result after the iteration with the original signal and makes the one whose amplitude is smaller as the initial value of the next *** method can eliminate the sidelobes produced by the intermediate operation,so that the following bandwidth extrapolation is more ***-MSVA is presented based on the modified SVA and modified super-SVA,which is suitable for any Nyquist sampling rate,can extrapolate the signal bandwidth many times through iteration with a commensurate improvement in resolution,as demonstrated by the result of the experiment.

关键词： synthetic aperture radar sidelobe suppression super-resolution SVA

基于RNA-seq对复合益生菌作用于肉鸡回肠中新转录本预测及可变剪接、SNP分析

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中国饲料 2024年第3期 20-27页

作者：曹威荣王燕飞张若男贾浩刘璇张利环朔州职业技术学院山西朔州036002 山西农业大学生命科学学院山西晋中030801

为分析复合益生菌饲喂肉鸡回肠转录组中的新转录本预测、可变剪接事件和SNP,选择200只1日龄黄麻肉鸡随机分为2组,每组5个重复,每个重复20只。对照组正常饮水,益生菌组饮用水中补充1%复合菌制剂,试验分为生长前期(1~21 d)、后期(22~42 d... 详细信息

为分析复合益生菌饲喂肉鸡回肠转录组中的新转录本预测、可变剪接事件和SNP,选择200只1日龄黄麻肉鸡随机分为2组,每组5个重复,每个重复20只。对照组正常饮水,益生菌组饮用水中补充1%复合菌制剂,试验分为生长前期(1~21 d)、后期(22~42 d)两个阶段。42日龄时进行肉鸡屠宰试验,取对照组和益生菌组回肠各3个样本进行转录组测序。对测序数据进行分析,共发现1047个新基因,20176个新转录本,276个新基因在GO数据库中得到归类注释。对转录组数据进行可变剪接分析,外显子跳跃(SE)占可变剪接类型的比例最高,共筛选到1131个显著的差异剪接基因,在外显子跳跃(SE)、第一个外显子可变剪接(A5SS)、最后一个外显子可变剪接(A3SS)、外显子选择性跳跃(MXE)、内含子滞留(RI)事件中鉴定到的差异剪接基因数分别为588、139、176、136、92。对获得的差异剪接基因进行GO富集分析,主要富集在代谢和免疫GO term。KEGG富集到了胞吞作用、MAPK信号通路等。在各样品中大多数碱基取代均为转换大于颠换,其中转换单核苷酸多态性(SNP)所占百分比为73.53%~73.94%;颠换型SNP所占百分比为26.05%~26.47%。试验对对照组、益生菌组肉鸡的回肠进行了RNA-seq测序分析,为发掘复合益生菌作用于肉鸡回肠中的可变剪接事件提供基础,为进一步了解复合益生菌作用于肉鸡回肠中新转录本的发现和SNP位点提供了数据支撑。

关键词：肉鸡 RNA-seq 新转录本 SNP 可变剪接

NUMERICAL ANALYSIS OF CENTERED-INCLINED COUPLING SLOTS IN RECTANGULAR WAVEGUIDES

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Journal of Electronics(China) 2014年第4期31卷 334-340页

作者： wang Xucun Zhou Yiguo wang yanfei Institute of Electronics Chinese Academy of Sciences University of Chinese Academy of Sciences

Numerical analysis is presented for the characteristic parameters of centered-inclined coupling slot in rectangular waveguides, taking into account the transverse distribution of the electric field across the slot aperture. Integral equations are formulated based on dyadic Green's function theories and solved using the method of moments. Trigonometric basis functions are adopted. It is found that the method will converge with up to ten basis functions. The characteristic parameters can be easily obtained for different slot sizes. Resonant length and resonant resistance of the coupling slots are calculated. It is shown that the calculated results have very high accuracy, compared with simulated results obtained from commercial software. Therefore, it can be effectively applied in the synthesis of antenna arrays. Effects of the transverse distribution on calculating resonant parameters of the coupling slot are also analyzed. The results show that if the transverse distribution of the electric field is neglected, the calculated error tends to become larger when the slot gets wider or thinner.

关键词： Rectangular waveguide Centered-inclined coupling slot Moment method Transverse distributionCLC number:TN823

Hierarchical strain band formation and mechanical behavior of a heterostructured dual-phase material

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Journal of Materials Science & Technology 2023年第31期162卷 25-37页

作者： Zhongkai Li Yujie Liu yanfei wang Jun Hu Lei Xu Jianjun wang Chunming Liu Yuntian Zhu Key Laboratory for Anisotropy and Texture of Materials of Ministry of Education Northeastern UniversityShenyang 110819China Department of Mechanics and Engineering Science College of EngineeringPeking UniversityBeijing 100871China The State Key Laboratory of Rolling and Automation Northeastern UniversityShenyang 110819China Shi-Changxu Innovation Center for Advanced Materials Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China Department of Materials Science and Engineering City University of Hong KongHong KongChina

Dispersive strain bands have been reported as a characteristic deformation feature of heterostructured materials,which helps to improve ***,their formation mechanism is still not well *** we report the formation of dispersed strain bands through dual-level hierarchical strain banding and its effect on the mechanical behavior of a heterostructured Fe-40Cu model ***,deformation started by the formation and propagation of dispersed microscale strain bands in the heterostructured Fe-40Cu *** strain gradient was generated within the microscale strain bands during their propagation and was accommodated by the accumulation of geometrically necessary disloca-tions(GNDs).The dispersed microscale strain bands were not uniformly distributed,but instead grouped together to form macroscale strain bands that were uniformly distributed over the entire gage section to accommodate the majority of the applied *** formation of this dual-level hierarchical strain bands prevented the formation of large strain localization to fail the sample *** was also found that increasing the strain hardening capacity of soft copper zones provides more room for the accumulation of GNDs,resulting in higher constraint to microscale strain band propagation and consequently higher *** observations suggest the possibility of tailoring microscale strain bands to optimize tensile performance of heterostructured materials.

关键词： Heterostructured materials Strain bands Strain gradient Hetero-deformation induced hardening

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科研团队实验室的自组织管理模式探索与实践

实验室研究与探索 2024年第4期43卷 194-198页

作者：赵文立王彦飞朱亮杨立斌贾原媛天津科技大学化工与材料学院天津300457

科研团队实验室作为研究生培养的主战场,具有开放时间长、人多物杂以及安全隐患多等特点,使实验室管理面临很大的挑战。工业结晶与颗粒过程研究团队近年来通过构建良好的团队文化,借助现代化的信息技术实现畅通的信息沟通机制,形成研究... 详细信息

科研团队实验室作为研究生培养的主战场,具有开放时间长、人多物杂以及安全隐患多等特点,使实验室管理面临很大的挑战。工业结晶与颗粒过程研究团队近年来通过构建良好的团队文化,借助现代化的信息技术实现畅通的信息沟通机制,形成研究生自我激活、自我管理、自我提升的“自组织”管理的模式。这种管理模式不仅高效利用现有实验室资源助力研究生的科研创新,同时提升了研究生的家国情怀、团结合作等综合素质。“自组织”的实验室管理模式不仅可以有效提高实验室管理绩效,培养的学生质量获得了行业的高度认可。通过实践探索,该管理模式具有较好的实践性和参考性,是一种可复制、可推广的科研团队实验室管理模式。

关键词：自组织科研团队团队文化实验室管理平台信息化工具

Growth performance,fatty acid composition,and lipid metabolism are altered in groupers(Epinephelus coioides)by dietary fish oil replacement with palm oil

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Animal Nutrition 2022年第1期8卷 102-113页

作者： Yingmei Qin Lingyun He yanfei wang Dong Li Weijun Chen Jidan Ye Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation Fisheries College of Jimei UniversityXiamen 361021China

In this study,we conducted a 56-d feeding trial to investigate the effects of replacing the fish oil(FO)with palm oil(PO)on the performance,tissue fatty acid(FA)composition,and mRNA levels of genes related to hepatic lipid metabolism in grouper(Epinephelus coioides).Five isolipidic(13%crude lipid)and isonitrogenous(48%CP)diets were formulated by incrementally adding PO to the control diet(25%fish meal and 9%added FO)to replace FO in the control *** groups of 30 groupers(initial weight:12.6±0.1 g)were fed one of the diets twice daily,to apparent *** replacement of FO with 50%PO revealed maximum growth without affecting the performance and whole-body proximate compositions,and replacing FO with 100%PO revealed a comparable(P>0.05)growth with that of the control diet,suggesting PO as a suitable alternative to *** analysis of FA profiles in the dorsal muscle and liver though reflected the FA profile of the diet,PO substitutions above 50%could compromise(P<0.05)the FA profile in the liver and flesh of the fish species in comparison with the control ***,the mRNA levels of FAS,G6PD,LPL,PPARА,and D6FAD genes in the liver had positive linear and/or quadratic responses,but the SCD,HSL,ATGL,FABP,SREBP-1C and ELOVL5 had the opposite trend,with increasing dietary PO inclusion levels,whereas the mRNA level of ACC was not affected by dietary *** optimal level of PO substitution for FO was estimated to be 47.1%of the feed,based on the regression analysis of percent weight gains against dietary PO inclusion levels;however,it might affect the FA profile in the liver and flesh of the fish species,and further study is required to investigate whether the changes in tissue FA composition will affect the welfare and market value over a production cycle of grouper.

关键词： Dietary lipid Epinephelus coioides Fatty acid metabolism Growth performance

潮气量挑战PPV与PPV在老年髋关节手术中对目标导向液体容量管理策略的影响研究

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新疆医科大学学报 2024年第3期47卷 388-392页

作者：李海丽王艳飞张小引东南大学医学院附属医院/南京同仁医院麻醉科南京211102

目的比较潮气量挑战脉压变异率(Volume challenge pulse pressure variation, VtPPV)和脉压变异率(Pulse pressure variation, PPV)两种液体目标导向管理方法对老年髋关节手术输液量以及预后的影响。方法以2020年3月-2023年3月在东南... 详细信息

目的比较潮气量挑战脉压变异率(Volume challenge pulse pressure variation, VtPPV)和脉压变异率(Pulse pressure variation, PPV)两种液体目标导向管理方法对老年髋关节手术输液量以及预后的影响。方法以2020年3月-2023年3月在东南大学医学院附属医院/南京同仁医院麻醉科全麻下行择期髋关节手术的100例患者为研究对象,采用随机数字表法分为潮气量挑战PPV(VtP组)和PPV组(P组),每组50例。VtP组:将潮气量从6 mL/kg增加到8 mL/kg并持续1 min,若ΔPPV≥3.5%,10 min内予以200 mL胶体液快速输注;并在液体预负荷结束前1 min内重复测量并干预。若连续2次显示容量欠佳,第二次输注晶体液200 mL,直至ΔPPV<3.5%。P组:潮气量为8 mL/kg时,若PPV≥11%持续1 min, 10 min内予以200 mL胶体液输注;并在液体预负荷结束前1 min内重复测量并干预。若连续2次显示容量欠佳,第二次输注晶体液200 mL,直至PPV<11%。记录患者术中低血压时间加权平均值(Time weighted average of Hypotension, TWA)、术毕血乳酸水平、输液量、出血量、尿量,术后48 h肺部、泌尿系统并发症发生率、住院时间以及术后24、48 h QoR-15满意度评分。记录患者入室、插管后、切皮时、以及术中不同时间点平均动脉压和心率的情况。结果与P组比较,VtP组术毕总输液量、晶体用量和血乳酸水平减少,胶体用量增多,手术开始60、90 min时平均动脉压增高,术中TWA降低,术后24 h恢复满意度评分增大,差异有统计学意义(P<0.05)。结论在老年髋关节手术中,基于潮气量挑战PPV的目标导向液体容量管理策略围术期低血压发生少,可降低术中输液量,提高患者满意度。

关键词：潮气量挑战脉压变化目标导向液体时间加权平均值髋关节置换术

Hydrogen embrittlement and failure mechanisms of multi-principal element alloys:A review

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Journal of Materials Science & Technology 2022年第27期122卷 20-32页

作者： Xinfeng Li Jing Yin Jin Zhang yanfei wang Xiaolong Song Yong Zhang Xuechong Ren Sino-French Institute of Nuclear Engineering and Technology Sun Yat-sen UniversityZhuhai 519082China College of Education for the Future Beijing Normal UniversityZhuhai 519087China School of Chemical Engineering&Technology China University of Mining and TechnologyXuzhou 221116China State Key Laboratory for Mechanical Behavior of Materials Xi’an Jiaotong UniversityXi’an 710049China State Key Laboratory for Advanced Metals and Materials University of Science and Technology BeijingBeijing 100083China National Center for Materials Service Safety University of Science and Technology BeijingBeijing 100083China

Multi-principal element alloys exhibit excellent physical,chemical and mechanical properties,and they are used as novel structural materials for potential applications in nuclear energy,hydrogen energy,and petrochemical ***,exposing components made of the alloys to service conditions related to the mentioned applications may induce hydrogen embrittlement(HE)as one of the typical failure *** this review,we report and summarize the progress in understanding HE in multi-principal element alloys,with a particular focus on high-entropy alloys(HEAs).The review focuses on four aspects:(1)hydrogen migration behavior(hydrogen dissolution,hydrogen diffusion,and hydrogen traps);(2)factors affecting HE(hydrogen concentration,alloy elements and microstructure);(3)tensile mechanical properties in the presence of hydrogen and micro-damage HE mechanisms;(4)the design concept for preventing hydrogen-induced mechanical *** differences in the HE behavior and failure mechanisms between HEAs and traditional alloys are compared and ***,specific research directions for further investigation of fundamental HE issues and a strategy for a simultaneous improvement in strength and HE resistance are identified.

关键词： Hydrogen embrittlement High-entropy alloy Hydrogen diffusion Mechanical property