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Microsystems & Nanoengineering 2020年第1期6卷 1187-1198页

作者： yanfang guan Baichuan Sun School of Electromechanical Engineering Henan University of technologyZhengzhou 450001China

As a promising concept,microfluidic paper-based analytical devices(μPADs)have seen rapid development in recent *** this study,a new method of fabricatingμPADs by atom stamp printing(ASP)is proposed and *** advantages of this new method compared to other methods include its low cost,ease of operation,high production efficiency,and high resolution(the minimum widths of the hydrophilic channels and hydrophobic barriers are 328 and 312μm,respectively).As a proof of concept,μPADs fabricated with the ASP method were used to detect different concentrations of Cu^(2+)via a colorimetric ***,combined with a distance-based detection method,these devices achieved a Cu^(2+)detection limit of down to 1mg/*** addition,a new paper-based solid–liquid extraction device(PSED)based on a three-dimensional(3D)μPAD with a“3+2”structure and a recyclable extraction mode was ***,using the characteristics of paper filtration and capillary force,the device completed multiple extraction and filtration steps from traditional solid–liquid extraction processes with high *** developed PSED platform allows the detection of heavy metal ions much more cheaply and simply and with a faster response time at the point of care,and it has great promise for applications in food safety and environmental pollution in resource-limited areas.

关键词： printing analytical faster

Analysis of Vibrational Performance of A Piezoelectric Micropump with Diffuse/Nozzle Microchannel

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Nanotechnology and Precision Engineering 2018年第2期1卷 138-144页

作者： yanfang guan Xiang Li School of Mechanical and Electrical Engineering Henan University of TechnologyZhengzhou 450001China

Piezoelectric transducers of different external diameters are designed and fabricated and incorporated into micropumps. Through finite element analysis, it is shown that the volume efficiency of the micropump reaches its maximum value for the first mode of vibration. The variation of maximum displacement with frequency is determined under free and forced vibration. Results demonstrate that this variation shows the same trend for different driving waves at the same driving voltage. The maximum displacement under forced vibration is less than that under free vibration. The displacement increases with decreasing distance from the center of the transducer. The maximum displacement is inversely proportional to the diameter of the transducer and proportional to the driving voltage under both free and forced vibrations. Finally, the micropump flow rate and pressure are measured and are found to manifest the same trend as the maximum displacement under the same driving conditions. For a piezoelectric transducer of 12 mm external diameter, the maximum flow rate and pressure value are 150 μL/min and 346 Pa, respectively, under sine-wave driving at 100 Vpp driving voltage.

关键词： Micropump Vibrational displacement Piezoelectric transducer Microflow

Clinical spectrum and prognostic value of TP53 mutations in circulating tumor DNA from breast cancer patients in China

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Cancer Communications 2020年第6期40卷 260-269页

作者： Zongbi Yi Fei Ma Guohua Rong yanfang guan Chunxiao Li Binghe Xu Department of Medical Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021P.R.China Geneplus-Beijing Institute Beijing 100021P.R.China State Key Laboratory of Molecular Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021P.R.China

Background:TP53 mutations are common in breast *** is currently no large-scale cohort study to investigate the TP53 landscape in breast cancer patients from *** predictive value of TP53 mutations for the efficacy of human epidermal growth factor receptor 2(HER2)-targeted therapy in breast cancer remains *** the present study,we aimed to analyze the clinical spectrum and prognostic value of TP53 mutations in circulating tumor DNA(ctDNA)from breast cancer patients in ***:We retrospectively analyzed the clinical data and TP53 mutation features in ctDNA samples from 804 patients withmetastatic breast ***53 mutations were detected by target region capture-based next-generation *** relationship between TP53 mutation status and disease-free survival(DFS)was analyzed in 444 patientswithmetastatic breast ***,the relationship between TP53 mutation status and progression-free survival(PFS)was analyzed in 55 HER2-positive patients treated with first-line trastuzumab-based ***-Meier analysis was performed to estimate the survival curves of the different subgroups,and the log-rank test was used to compare the curves.A Cox regression model was used to estimate multivariable-adjusted hazard ratios and their 95%confidence intervals(CIs)associated with the DFS and ***:Among the 804 investigated patients,431(53.6%)patients harbored TP53 ***53 mutations were differentially distributed among different molecular subtypes of breast cancer(P<0.05).Patients with TP53 mutations had a shorter DFS than those with wild-type TP53(hazard ratio=1.32,95%CI=1.09-1.61,P=0.005).TP53 mutations in exons 5-8 were associated with worse outcome(hazard ratio=1.50,95%CI=1.11-2.03,P=0.009).However,TP53 mutation status was not significantly associated with PFS in HER2-positive patients who received firstline trastuzumab-based therapy(P=0.966).Interestingly,in the taxane combination group,patients with TP53 mutations

关键词： breast cancer TP53 mutation circulating tumor DNA next-generation sequencing Chinese prognosis trastuzumab taxanes

The molecular tumor burden index as a response evaluation criterion in breast cancer

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Signal Transduction and Targeted Therapy 2021年第8期6卷 2494-2501页

作者： Zongbi Yi Fei Ma Guohua Rong Binliang Liu yanfang guan Jin Li Xiaoying Sun Wenna Wang Xiuwen guan Hongnan Mo Jiani Wang Haili Qian Binghe Xu Department of Medical Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Geneplus-Beijing Institute BeijingChina Department of Medical Oncology Huanxing Cancer HospitalBeijingChina State Key Laboratory of Molecular Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina

Circulating tumor DNA(ctDNA)is a potential biomarker of prognosis and therapeutic *** conducted this study to explore the role of the molecular tumor burden index(mTBI)in ctDNA as a therapeutic response and prognostic biomarker in a larger cohort prospective phase III randomized multicenter *** collected 291 plasma samples from 125 metastatic breast cancer patients from the CAMELLIA study(NCT01917279).Target-capture deep sequencing of 1021 genes was performed to detect somatic variants in ctDNA from the plasma *** pretreatment mTBI value was correlated with tumor burden(P=0.025).Patients with high-level pretreatment mTBI had shorter overall survival than patients with low-level pretreatment mTBI,and the median overall survival was 40.9 months and 68.4 months,respectively(P=0.011).Patients with mTBI decrease to less than 0.02%at the first tumor evaluation had longer progression-free survival and overall survival(P<0.001 and P=0.007,respectively).The mTBI has good sensitivity to identify complete response/partial response and progressive disease based on computed tomography scans(88.5%and 87.5%,respectively).The patients classified as molecular responders had longer progression-free survival and overall survival than the nonmolecular responders in the overall cohort(P<0.001 and P=0.036,respectively),as well as in the cohort in which computed tomography scans were defined as representing stable disease(P=0.027 and P=0.015,respectively).The mTBI in ctDNA detected in liquid biopsies is a potential biomarker of therapeutic response and prognosis in patients with metastatic breast cancer.

关键词： breast cancer index

Mutational characteristics determined using circulating tumor DNA analysis in triple-negative breast cancer patients with distant metastasis

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Cancer Communications 2020年第12期40卷 738-742页

作者： Guohua Rong Zongbi Yi Fei Ma yanfang guan Yaping Xu Lifeng Li Binghe Xu Department of Medical Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021P.R.China Geneplus-Beijing Institute Beijing 100021P.R.China

Dear editor,Breast cancer has been considered as the most common malignancy and the leading cause of death in women worldwide[1].Triple-negative breast cancer(TNBC)accounts for 10%-20%of breast cancers,which are characterized by the absence of estrogen receptor(ER),progesterone receptor(PR),and amplification of human epidermal growth factor receptor 2(HER2)[2].TNBC is well known for its rapid progressiveness,high rate of distant organ recurrence,and poor *** remains the major cause of death for patients with *** to date,the only direct treatment for metastatic TNBCs is chemotherapy,which has been challenged by tumor heterogeneity and drug *** of effective targeted therapy for metastatic TNBCs impels researchers to focus on discovering potentially actionable targets through mutational profiling.

关键词： breast patients metastasis

Preferable background filtering for next-generation sequencing analysis in non-small cell lung cancer:pericarcinomatous tissues or peripheral blood lymphocytes?

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Cancer Communications 2019年第1期39卷 314-317页

作者： Yaxiong Zhang Lianpeng Chang Wenfeng Fang Yunpeng Yang Lanjun Zhang Shaodong Hong Huaqiang Zhou yanfang guan Xin Yi Li Zhang State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineSun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Department of Medical Oncology Sun Yat-sen University Cancer Center651 Dongfeng Road EastGuangzhou 510060GuangdongP.R.China Geneplus-Beijing Institute Beijing 100000P.R.China Department of Thoracic Surgery Sun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China

Dear editor,Lung cancer is the leading cause of cancer-related death worldwide,with the predominant pathological type being non-small cell lung cancer(NSCLC)[1,2].Next-gener-ation sequencing(NGS)analysis is increasingly used to help clinicians select appropriate target therapies,such as epidermal growth factor receptor-tyrosine kinase inhibitors(EGFR-TKIs)for EGFR-mutant patients[3].Both pericarcinomatous tissues and peripheral blood lymphocytes are widely used as normal control for NGS ***,whether pericarcinomatous tissue is suitable for background filtering in mutation analysis remains *** to the whole-genome sequencing data from The Cancer Genome Atlas(TCGA)database,there were some genomic variations in peri-carcinomatous tissue from NSCLC patients,but no driver gene mutation was detected[4,5].Therefore,deep sequencing of pericarcinomatous and tumor tissues is necessary to confirm whether pericarcinomatous tissue harbors low-frequency mutations.

关键词： lymphocytes lung filtering

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农村土地信托助力农业供给侧结构性改革研究

改革与战略 2017年第12期33卷 127-129,160页

作者：管延芳辽宁大学国际关系学院辽宁沈阳110000 吉林师范大学经济学院吉林四平136000

土地信托作为一种新兴的土地金融产品,与农业供给侧结构性改革在核心目标、规模经济、全面统筹和优化配置等方面存在天然的契合性。农村土地信托在其追求自我完善的过程中,可以通过提高农产品产量、优化农产品结构、推进农业绿色发展和... 详细信息

土地信托作为一种新兴的土地金融产品,与农业供给侧结构性改革在核心目标、规模经济、全面统筹和优化配置等方面存在天然的契合性。农村土地信托在其追求自我完善的过程中,可以通过提高农产品产量、优化农产品结构、推进农业绿色发展和推进"六次产业"发展等方面,助力中国农业供给侧结构性改革,实现中国农村土地信托发展与农业供给侧结构性改革的共赢。

关键词：农村土地信托土地金融农业供给侧结构性改革

Molecular features of gastroenteropancreatic neuroendocrine carcinoma: A comparative analysis with lung neuroendocrine carcinoma and digestive adenocarcinomas

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Chinese Journal of Cancer Research 2024年第1期36卷 90-102页

作者： Jianwei Zhang Hanxiao Chen Junli Zhang Sha Wang yanfang guan Wenguang Gu Jie Li Xiaotian Zhang Jian Li Xicheng Wang Zhihao Lu Jun Zhou Zhi Peng Yu Sun Yang Shao Lin Shen Minglei Zhuo Ming Lu Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Department of Gastrointestinal OncologyPeking University Cancer Hospital&InstituteBeijing 100142China Department of Radiation Therapy National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital&Shenzhen HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeShenzhen 518172China Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Department I of Thoracic OncologyPeking University Cancer Hospital&InstituteBeijing 100142China Medical Department Nanjing Geneseeq Technology Inc.Nanjing 210061China Geneplus-Beijing Beijing 102200China State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers Beijing Key Laboratory of Carcinogenesis and Translational ResearchDepartment of Gastrointestinal OncologyPeking University Cancer Hospital&InstituteBeijing 100142China Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Department of PathologyPeking University Cancer Hospital&InstituteBeijing 100142China

Objective: There is an ongoing debate about whether the management of gastroenteropancreatic(GEP)neuroendocrine carcinoma(NEC) should follow the guidelines of small-cell lung cancer(SCLC). We aim to identify the genetic differences of GEPNEC and its ***: We recruited GEPNEC patients as the main cohort, with lung NEC and digestive adenocarcinomas as comparative cohorts. All patients undergone next-generation sequencing(NGS). Different gene alterations were compared and analyzed between GEPNEC and lung NEC(LNEC), GEPNEC and adenocarcinoma to yield the remarkable ***: We recruited 257 patients, including 99 GEPNEC, 57 LNEC, and 101 digestive *** the mutations, KRAS, RB1, TERT, IL7R, and CTNNB1 were found to have different gene alterations between GEPNEC and LNEC samples. Specific genes for each site were revealed: gastric NEC(TERT amplification),colorectal NEC(KRAS mutation), and bile tract NEC(ARID1A mutation). The gene disparities between small-cell NEC(SCNEC) and large-cell NEC(LCNEC) were KEAP1 and CDH1. Digestive adenocarcinoma was also compared with GEPNEC and suggested RB1, APC, and KRAS as significant genes. The TP53/RB1 mutation pattern was associated with first-line effectiveness. Putative targetable genes and biomarkers in GEPNEC were identified in22.2% of the patients, and they had longer progression-free survival(PFS) upon targetable treatment [12.5 months vs. 3.0 months, HR=0.40(0.21-0.75), P=0.006].Conclusions: This work demonstrated striking gene distinctions in GEPNEC compared with LNEC and adenocarcinoma and their clinical utility.

关键词： Neuroendocrine carcinoma gastroenteropancreatic lung genetic alterations molecular markers

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分业经营模式下人寿保险信托产品的发展途径

长春大学学报 2016年第5期26卷 32-36页

作者：管延芳吉林师范大学经济学院吉林四平136000

在分业经营模式下,人寿保险信托产品作为一种保险和信托组合的新产品悄然出现。从信托财产的角度分析了人寿保险信托产品的递进性及其共赢模式,针对人寿保险信托产品发展中存在的法律空白、兑付危机、教育短板、监管分立等问题,提出了... 详细信息

在分业经营模式下,人寿保险信托产品作为一种保险和信托组合的新产品悄然出现。从信托财产的角度分析了人寿保险信托产品的递进性及其共赢模式,针对人寿保险信托产品发展中存在的法律空白、兑付危机、教育短板、监管分立等问题,提出了制定并完善相关法律法规、提高信托机构内控水平、宣扬信托理念、调整监管体系等发展途径。

关键词：分业经营人寿保险信托发展途径

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全合一混合液的配制和使用管理

护理研究 2005年第21期19卷 1938-1940页

作者：关艳芳山西省汾阳医院

全合一(all in one,AIO)混合液是胃肠外营养支持治疗中一种重要输注方式。本文依据10年工作实践并参考相关文献,针对AIO配制过程中防止微生物污染、保证配伍稳定性,及AIO使用过程中建立合适的静脉通道,输注过程中更换营养液、封管,常见... 详细信息

全合一(all in one,AIO)混合液是胃肠外营养支持治疗中一种重要输注方式。本文依据10年工作实践并参考相关文献,针对AIO配制过程中防止微生物污染、保证配伍稳定性,及AIO使用过程中建立合适的静脉通道,输注过程中更换营养液、封管,常见并发症的观察与护理等相关各方面内容进行总结,为临床护理工作者配制和使用AIO提出一套相对完整的工作规程和注意事项。

关键词：全合一混合液配制使用管理