检索结果-南通市图书馆

Prediction of single cell mechanical properties in microchannels based on deep learning

在线全文

学校读者我要写书评

暂无评论

Applied Mathematics and Mechanics(English Edition) 2024年第11期45卷 1857-1874页

作者： Jiajie GONG Xinyue LIU yancong zhang Fengping ZHU Guohui HU Shanghai Institute of Applied Mathematics and Mechanics School of Mechanics and Engineering ScienceShanghai Frontier Science Center of MechanoinformaticsShanghai Key Laboratory of Mechanics in Energy EngineeringShanghai UniversityShanghai200072China Shanghai Institute of Aircraft Mechanics and Control Shanghai200092China Department of Neurosurgery Huashan HospitalShanghai Medical CollegeFudan UniversityShanghai200040China

Traditional methods for measuring single-cell mechanical characteristics face several challenges,including lengthy measurement times,low throughput,and a requirement for advanced technical *** overcome these challenges,a novel machine learning(ML)approach is implemented based on the convolutional neural networks(CNNs),aiming at predicting cells'elastic modulus and constitutive equations from their deformations while passing through micro-constriction *** the present study,the computational fluid dynamics technology is used to generate a dataset within the range of the cell elastic modulus,incorporating three widely-used constitutive models that characterize the cellular mechanical behavior,i.e.,the Mooney-Rivlin(M-R),Neo-Hookean(N-H),and Kelvin-Voigt(K-V)*** this dataset,a multi-input convolutional neural network(MI-CNN)algorithm is developed by incorporating cellular deformation data as well as the time and positional *** approach accurately predicts the cell elastic modulus,with a coefficient of determination R^(2)of 0.999,a root mean square error of 0.218,and a mean absolute percentage error of 1.089%.The model consistently achieves high-precision predictions of the cellular elastic modulus with a maximum R^(2)of 0.99,even when the stochastic noise is added to the simulated *** significant feature of the present model is that it has the ability to effectively classify the three types of constitutive equations we *** model accurately and reliably predicts single-cell mechanical properties,showcasing a robust ability to *** demonstrate that incorporating deformation features at multiple time points can enhance the algorithm's accuracy and *** algorithm presents a possibility for high-throughput,highly automated,real-time,and precise characterization of single-cell mechanical properties.

关键词： cell deformation single-cell mechanics machine learning(ML) constitutive law convolutional neural network(CNN)

Bidirectional solid fermentation using Trametes robiniophila Murr. For enhancing efficacy and reducing toxicity of rhubarb (Rheum palmatum L.)

在线全文

学校读者我要写书评

暂无评论

Journal of Traditional Chinese Medical Sciences 2017年第3期4卷 306-313页

作者： yancong zhang Li Zhou Weiwei Ma Xiaosa Shi Hongmei zhang Xinyuan Shi School of Chinese Materia Medica Beijing University of Chinese MedicineBeijing 100102China

Objective:To investigate the potential of bidirectional solid fermentation of rhubarb (Rheum palmatum L.) for reducing its toxicity and enhancing its medicinal ***:The fungus Trametes robiniophila *** inoculated into *** chemical ingredients as well as antioxidant,antibacterial,and anticancer activities of fermented and unfermented rhubarb extracts were then ***:After fermentation,levels of anthraquinone glycosides (purgative ingredients) decreased significantly,while the level of anthraquinone aglycone *** level of gallic acid was also reduced after *** extract of rhubarb (0.8 mg/mL) exhibited DPPH-scavenging activity of 7.6% ± 0.8% while the blank control (0.8 mg/mL rhubarb)showed 31.3% ± 2.0% *** activities in fermented samples were found to be enhanced compared with unfermented samples and anticancer activity was evident at concentrations of 2000 and 5000 μg/***:Bidirectional solid fermentation appears to be an effective processing method that can be used to improve the efficacy and reduce the toxicity of rhubarb.

关键词： Rhubarb Trametes robiniophila Murr. Bidirectional solid fermentation Anthraquinones Cancer inhibition

Illumina MiSeq sequencing investigation of Chanhua (Cordyceps cicadae Shing) fungal community structures in different regions

在线全文

学校读者我要写书评

暂无评论

Journal of Traditional Chinese Medical Sciences 2018年第2期5卷 206-212页

作者： Tengfei Liu Hongmei zhang Xiaosa Shi Weiwei Ma yancong zhang Ziyao Liu Ying Huang Xinyuan Shi School of Chinese Materia Medica Beijing University of Chinese MedicineBeijing 100102China

Objective:This study was carried out to elucidate the fungal diversity of Chanhua (Cordyceps cicadae Shing),which is an entomogenous fungal parasite that grows on wingless cicada ***:Samples were collected from 10 different geographical regions including Yibing in Sichuan Province,Jiangsu Province,Jiangxi Province,Anhui Province,Guangdong Province,and Guangxi Autonomous *** Illumina MiSeq-based method was used to investigate the Chanhua fungal community ***:The results showed high fungal diversity,with 22 families found in the different samples,Eurotiomycetes was the dominant fungal family associated with Chanhua (composing 36.69% of the sample from Sichuan Mianyang),whereas Cordycipitaceae only composed 0.51% (Sichuan Yibing) to 0.33% (Guangdong) of these ***,the proportion of Capnodiales was 5.27% in Sichuan Mianyang,whereas the percentage in other regions ranged from 4.35% (Sichuan Yibing) to 2.55% (Zhejiang),and the remaining proportion (more than 80%) was made up of unidentified ***:These findings contribute to a better understanding of Chanhua from different regions and revealed an unexpectedly and high amount of unidentified diversity of these fungal *** results indicate that Chanhua parasitic fungi need to be further analyzed and identified for finding new medical fungi resources.

关键词： Different habitats Fungal diversity Chanhua

The Genome of Medicinal Plant Macleaya cordata Provides New Insights into Benzylisoquinoline Alkaloids Metabolism

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2017年第7期10卷 975-989页

作者： Xiubin Liu Yisong Liu Peng Huang Yongshuo Ma Zhixing Qing Qi Tang Huifen Cao Pi Cheng Yajie Zheng Zejun Yuan Yuan Zhou Jinfeng Liu Zhaoshan Tang Yixiu Zhuo yancong zhang Linlan Yu Jialu Huang Peng Yang Qiong Peng dinbo zhang Wenkai Jiang Zhonghua zhang Kui Lin Dae-Kyun Ro Xiaoya Chen Xingyao Xiong Yi Shang Sanwen Huang Jianguo Zeng National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resource and Initiative Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients Horticulture and Landscape College Veterinary Medicine College Hunan Agricultural University Changsha 410128 China Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture Sino-Dutch Joint Laboratory of Horticultural Genomics Beijing 100081 China Agricuitural Genome Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen 518124 China School of Pharmacy Hunan University of Chinese Medicine Changsha 410208 China MOE Key Laboratory for Biodiversity Science and Ecological Engineering and College of Life Sciences Beijing Normal University Beijing 100875 China Micolta Bioresource Inc. Changsha 410016 China Herbal Extract Engineering Research Center Changsha 410331 China Biotechnology Research Center Hunan Academy of Agricultural Sciences Changsha 410125 China Novogene Bioinformatics Institute Beijing 100083 China Department of Biological Sciences University of Calgary Calgary T2N1N4 Canada National Key Laboratory of Plant Molecular Genetics National Plant Gene Research Center Institute of Plant Physiology and Ecology Shanghai Institutes forBiological Sciences Chinese Academy of Sciences Shanghai 200032 China Plant Science Research Center Shanghai Chenshan Botanical Garden Shanghai 201602 China

The overuse of antibiotics in animal agriculture and medicine has caused a series of potential threats to public health. Macleaya cordata is a medicinal plant species from the Papaveraceae family, providing a safe resource for the manufacture of antimicrobial feed additive for livestock. The active constituents from M. cordata are known to include benzylisoquinoline alkaloids （BIAs） such as sanguinarine （SAN） and chelerythrine （CHE）, but their metabolic pathways have yet to be studied in this non-model plant. The active biosynthesis of SAN and CHE in M. cordata was first examined and confirmed by feeding ^13C-labeled tyrosine. To gain further insights, we de novo sequenced the whole genome of M. cordata, the first to be sequenced from the Papaveraceae family. The M. cordata genome covering 378 Mb encodes 22,328 predicted protein-coding genes with 43.5% being transposable elements. As a member of basal eudicot, M. cordata genome lacks the paleohexaploidy event that occurred in almost all eudicots. From the genomics data, a complete set of 16 metabolic genes for SAN and CHE biosynthesis was retrieved, and 14 of their biochemical activities were validated. These genomics and metabolic data show the conserved BIA metabolic pathways in M. cordata and provide the knowledge foundation for future productions of SAN and CHE by crop improvement or microbial pathway reconstruction.

关键词： Macleaya cordata genome Benzylisoquinoline alkaloids Secondary metabolism Sanguinarine Chelerythrine Isotopic labeling

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

刚柔复合式路面裂缝反射预防技术研究进展

材料导报 2016年第3期30卷 86-90,104页

作者：刘志胜刘志岗张翛张艳聪山西省交通科学研究院黄土地区公路建设与养护技术交通行业重点实验室太原030006

为系统掌握国内外有关刚柔复合式路面裂缝反射预防技术的研究现状,通过调查现有层间裂缝防治材料的设计思路、性能试验、应用效果,评述针对旧水泥混凝土路面加铺层裂缝反射预防材料的优缺点,总结现有裂缝反射预防技术存在的问题及发展... 详细信息

为系统掌握国内外有关刚柔复合式路面裂缝反射预防技术的研究现状,通过调查现有层间裂缝防治材料的设计思路、性能试验、应用效果,评述针对旧水泥混凝土路面加铺层裂缝反射预防材料的优缺点,总结现有裂缝反射预防技术存在的问题及发展趋势。结果表明:国内外全幅式、条带式裂缝预防技术已经应用颇多,取得了一定的效果,但关于裂缝反射预防材料的技术规范相对缺乏,条带式-复合型裂缝预防措施将在未来道路工程中常态化应用。现浇、条带式应力吸收带在抗拉性能、经济方面效果较好,可在未来旧水泥混凝土路面加铺沥青层时参考应用。

关键词：复合式路面裂缝反射研究现状发展趋势

PLGA静电纺丝纳米纤维结合诱导培养基和BMP4诱导鼠胚胎干细胞向牙上皮分化

在线全文

学校读者我要写书评

暂无评论

实用口腔医学杂志 2021年第1期37卷 19-23页

作者：张彦聪窦林波张风河山东大学口腔医学院 250000 德州市人民医院

目的:检测聚羟基乙酸聚乳酸共聚物(PLGA)纳米支架材料结合诱导培养基和BMP4对胚胎鼠干细胞(mES)向牙上皮细胞分化的影响。方法:用PLGA作为基底材料,通过静电纺丝技术构建纳米纤维支架材料,结合不同时间点BMP4的干预,将鼠胚胎干细胞向牙... 详细信息

目的:检测聚羟基乙酸聚乳酸共聚物(PLGA)纳米支架材料结合诱导培养基和BMP4对胚胎鼠干细胞(mES)向牙上皮细胞分化的影响。方法:用PLGA作为基底材料,通过静电纺丝技术构建纳米纤维支架材料,结合不同时间点BMP4的干预,将鼠胚胎干细胞向牙上皮细胞诱导,构建mES向牙上皮分化的诱导体系,并进一步筛选出mES细胞向牙上皮分化的最佳诱导方案。结果:在培养初期抑制BMP448 h,然后在培养液中短时间(48 h)或持续加入外源性BMP4,48 h后可检测到成釉蛋白(AMBN)阳性的细胞。结论:PLGA纳米纤维表面结合诱导培养基和BMP4可将mES细胞诱导为牙上皮细胞。

关键词：鼠胚胎干细胞牙上皮细胞 BMP4 支架材料

分斋与序位:宋代太学“分斋教学”的空间诠释

在线全文

学校读者我要写书评

暂无评论

中国教育：研究与评论 2020年第1期 101-147页

作者：张彦聪河南财经政法大学社会学院

教育序位是指对受教育个体的空间性(同时意味着时间性)进行界定,由序位界定的日常教育实践在社会时空特性上表现出某些结构性特征,故而教育序位实质上是中国古代空间实践中孕育的一种特殊的社会时空结构。本文从这一视角对宋代太学"分... 详细信息

教育序位是指对受教育个体的空间性(同时意味着时间性)进行界定,由序位界定的日常教育实践在社会时空特性上表现出某些结构性特征,故而教育序位实质上是中国古代空间实践中孕育的一种特殊的社会时空结构。本文从这一视角对宋代太学"分斋教学"进行诠释,从而澄清其历史真相。伴随唐宋以来的变革,太学经历了影响深远的空间重构,并通过"分斋"建构了一种新型教育序位体制,由此形塑了"斋院"这一独特的建筑空间形态及其日常教育生活。"分斋序位体制"表现出密藏与敞显两种矛盾的空间性,根源于宋代太学养士选士一体化的内在矛盾。不同改革派别针对这一矛盾采取了不同的空间策略。由于这一矛盾始终没有得到有效解决,一方面直接导致了太学教育的腐化堕落,另一方面则间接加剧了官学与书院的分化与对立。

关键词：分斋教学教育序位空间矛盾太学宋代胡瑗

在线全文

学校读者我要写书评

暂无评论

不同养生方式下水泥混凝土路面早期开裂风险

辽宁工程技术大学学报（自然科学版） 2013年第12期32卷 1677-1680页

作者：张艳聪高玲玲山西省交通科学研究院黄土地区公路建设与养护技术交通行业重点实验室山西太原030006 山西水利职业技术学院道路与桥梁工程系山西运城044004

针对水泥混凝土路面的早期养生问题.选择液体养生剂覆盖养生和PE薄膜、麻布保水养生等6种方式对混凝土面层进行养生,基于HIPERPAV软件,分别测算铺筑72 h内不同养生方式下面层弯拉强度和最大应力的发展规律.结果表明:面层铺筑后的第13 h... 详细信息

针对水泥混凝土路面的早期养生问题.选择液体养生剂覆盖养生和PE薄膜、麻布保水养生等6种方式对混凝土面层进行养生,基于HIPERPAV软件,分别测算铺筑72 h内不同养生方式下面层弯拉强度和最大应力的发展规律.结果表明:面层铺筑后的第13 h,应力强度比达到第一次峰值.此时,水泥水化是结构应力急剧增加的关键因素,应力强度比的发展趋势与气温变化曲线基本吻合,水化热不在起主导作用.铺筑后的第13~24 h是早期裂缝的风险高发期,该时段是混凝土水化热高速积聚期,若环境温度也处于高位,则路面随时面临最大应力超出弯拉强度而产生开裂的风险.为降低该风险,应避免采用覆盖后不补水的养生措施,在水源充足的条件下,宜采用覆盖物及时保水并上覆PE薄膜的养生方式.

关键词：道路工程养生方式应力强度比水泥混凝土路面早期开裂

在线全文

学校读者我要写书评

暂无评论

药物引起色素沉着126例文献分析

天津药学 2007年第6期19卷 25-27页

作者：张艳丛天津市海河医院天津300350

目的:探讨药物引起色素沉着的特点及一般规律。方法:对1994年1月—2006年12月国内医药学术期刊报道的药物引起的色素沉着126例文献进行统计和分析。结果:药物引起的色素沉着可出现在给药过程中,也可以出现在连续用药后;引起色素沉着的... 详细信息

目的:探讨药物引起色素沉着的特点及一般规律。方法:对1994年1月—2006年12月国内医药学术期刊报道的药物引起的色素沉着126例文献进行统计和分析。结果:药物引起的色素沉着可出现在给药过程中,也可以出现在连续用药后;引起色素沉着的药物种类以抗感染药和中药制剂居多。结论:临床应重视药物引起的色素沉着,以确保用药安全。

关键词：药品不良反应色素沉着文献分析