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利用中华猕猴桃杂交后代转录组测序筛选抗溃疡病相关基因

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园艺学报 2024年第8期51卷 1743-1757页

作者：匡美美李黎马建伟刘原蒋鸿霏雷瑞满玉萍王一帆黄波王彦昌刘世彪吉首大学生物资源与环境科学学院湖南吉首416000 中国科学院武汉植物园武汉430074 四川省苍溪猕猴桃研究所四川苍溪628400 中国农业大学农学院北京100193

猕猴桃细菌性溃疡病是猕猴桃产业中的毁灭性病害。不同遗传背景的猕猴桃材料抗性差异极大。为研究猕猴桃响应溃疡病菌(Pseudomonas syringae ***,Psa)的早期反应,挖掘抗病相关基因,对接种溃疡病菌6和24h后的中华猕猴桃‘金农’×中华猕... 详细信息

猕猴桃细菌性溃疡病是猕猴桃产业中的毁灭性病害。不同遗传背景的猕猴桃材料抗性差异极大。为研究猕猴桃响应溃疡病菌(Pseudomonas syringae ***,Psa)的早期反应,挖掘抗病相关基因,对接种溃疡病菌6和24h后的中华猕猴桃‘金农’×中华猕猴桃雄株杂交后代中的抗病个体‘21-2-4’及感病个体‘19-3-11’进行转录组测序比较分析。结果表明抗病个体对Psa防御主要集中在24 h,而感病个体主要集中在6 h。KEGG通路分析表明差异表达基因主要富集于植物—病原菌相互作用、苯丙烷生物合成、淀粉和蔗糖代谢途径等初生、次生产物的代谢。对比抗、感病个体中的基因表达模式,对植物—病原菌相互作用、植物激素信号转导及苯丙烷生物合成等抗病相关途径中可能的候选基因进行初步筛选。以中华猕猴桃抗性品种‘金农’和感病品种‘红昇’为材料,通过qRT-PCR验证了初步筛选出来的基因,确定PR1(Acc06864/Acc06865/Acc06866)、FLS2(Acc06484)可作为抗病候选基因深入研究。

关键词：中华猕猴桃细菌性溃疡病杂交后代转录组抗病相关基因

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Ultrafast micro/nano-manufacturing of metastable materials for energy

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National Science Review 2024年第4期11卷 283-298页

作者： Xiaoya Cui yanchang Liu Yanan Chen School of Materials Science and Engineering Key Laboratory of Advanced Ceramics and Machining Technology(Ministry of Education) and Tianjin Key Laboratory of Composite and Functional MaterialsTianjin University Ministry of Education Key Laboratory of Protein Sciences School of Life SciencesTsinghua University

The structural engineering of metastable nanomaterials with abundant defects has attracted much attention in energy-related fields. The high-temperature shock(HTS) technique, as a rapidly developing and advanced synthesis strategy, offers significant potential for the rational design and fabrication of high-quality nanocatalysts in an ultrafast, scalable, controllable and eco-friendly way. In this review, we provide an overview of various metastable micro-and nanomaterials synthesized via HTS, including single metallic and bimetallic nanostructures, high entropy alloys, metal compounds(e.g. metal oxides) and carbon nanomaterials. Note that HTS provides a new research dimension for nanostructures, i.e. kinetic modulation. Furthermore, we summarize the application of HTS—as supporting films for transmission electron microscopy grids—in the structural engineering of 2D materials, which is vital for the direct imaging of metastable materials. Finally, we discuss the potential future applications of high-throughput and liquid-phase HTS strategies for non-equilibrium micro/nano-manufacturing beyond energy-related *** is believed that this emerging research field will bring new opportunities to the development of nanoscience and nanotechnology in both fundamental and practical aspects.

关键词： high-temperature shock technique defect engineering metastable phase kinetic modulation non-equilibrium micro/nano-manufacturing

Identification of anti-Gram-negative bacteria agents targeting the interaction between ribosomal proteins L12 and L10

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Acta Pharmaceutica Sinica B 2018年第5期8卷 772-783页

作者： Weiwei wang Chao Liu Ningyu Zhu Yuan Lin JiANDong Jiang yanchang wang Yan Li Shuyi Si Beijing Key Laboratory of Antimicrobial Agents Institute of Medicinal Biotechnology Peking Union Medical College and Chinese Academy of Medical Sciences State Key Laboratory of Bioactive Substances and Function of Natural Medicine Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Department of Biomedical Sciences College of Medicine Florida State University

Gram-negative bacteria have become the main pathogens and cause serious clinical problems with increased morbidity and mortality. However, the slow discovery of new antimicrobial agents is unable to meet the need for the treatment of bacterial infections caused by drug-resistant strains. The interaction of L12 and L10 is essential for ribosomal function and protein synthesis. In this study, a yeast two-hybrid system was established to successfully detect the interaction between L12 and L10 proteins from gram-negative bacteria Escherichia coli, which allows us to screen compounds that specifically disrupt this interaction. With this system, we identified two compounds IMB-84 and IMB-87 that block L12-L10 interaction and show bactericidal activity against E. coli. We used glutathione-S-transferase(GST) pull-down and surface plasmon resonance(SPR) assays to demonstrate that these compounds disrupt L12-L10 interaction in vitro and the target of compounds was further confirmed by the overexpression of target proteins. Moreover, protein synthesis and elongation factor G-dependent GTPase activities are inhibited by two compounds. Therefore, we have identified two antibacterial agents that disrupt L12-L10 interaction by using yeast two-hybrid system.

关键词： Yeast two-hybrid Escherichia coli Ribosome L12/L10 Antimicrobial agents

General anesthetic agents induce neurotoxicity through astrocytes

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Neural Regeneration Research 2024年第6期19卷 1299-1307页

作者： yanchang Yang Tiantian Liu Jun Li Dandan Yan Yuhan Hu Pin Wu Fuquan Fang Patrick M.McQuillan Wenxin Hang Jianhang Leng Zhiyong Hu Department of Anesthesiology The First Affiliated Hospital Zhejiang University School of Medicine Department of Anesthesiology Ningbo Women and Children's Hospital 3. Department of Anesthesiology Shulan (Hangzhou) Hospital Affiliated to Zhejiang Shuren University Shulan International Medical College 4. Cell Biology Department Yale University 5. Department of Anesthesiology Penn State Hershey Medical Centre Penn State College of Medicine 6. Department of Central Laboratory Affiliated Hangzhou First People's Hospital Zhejiang University School of Medicine

Neuroscientists have recognized the importance of astrocytes in regulating neurological function and their influence on the release of glial transmitters. Few studies, however, have focused on the effects of general anesthetic agents on neuroglia or astrocytes. Astrocytes can also be an important target of general anesthetic agents as they exert not only sedative, analgesic, and amnesic effects but also mediate general anesthetic-induced neurotoxicity and postoperative cognitive dysfunction. Here, we analyzed recent advances in understanding the mechanism of general anesthetic agents on astrocytes, and found that exposure to general anesthetic agents will destroy the morphology and proliferation of astrocytes, in addition to acting on the receptors on their surface, which not only affect Ca2+signaling, inhibit the release of brain-derived neurotrophic factor and lactate from astrocytes, but are even involved in the regulation of the pro-and anti-inflammatory processes of astrocytes. These would obviously affect the communication between astrocytes as well as between astrocytes and neighboring neurons, other neuroglia, and vascular cells. In this review, we summarize how general anesthetic agents act on neurons via astrocytes, and explore potential mechanisms of action of general anesthetic agents on the nervous system. We hope that this review will provide a new direction for mitigating the neurotoxicity of general anesthetic agents.

关键词： astrocytes brain-derived neurotrophic factor general anesthetic agents neuron neurotoxicity N-methyl-D-aspartate receptor perioperative neurocognition Toll-like receptor γ-aminobutyric acid receptor

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多电源并联运行的效率优化控制方法

电力系统自动化 2018年第17期42卷 96-103,199页

作者：张强王言畅王锐哈尔滨工程大学自动化学院黑龙江省哈尔滨市150001 中兴通讯股份有限公司广东省深圳市518001

目前,由多个电源并联运行所构成的大容量电源系统,多采用的是均流控制策略来实现其内部多个电源单体间的负荷分配。均流控制虽然可以提高电源系统运行的可靠性,但是由于各个电源单体效率特性的差异,致使电源系统在运行过程中的总体效率... 详细信息

目前,由多个电源并联运行所构成的大容量电源系统,多采用的是均流控制策略来实现其内部多个电源单体间的负荷分配。均流控制虽然可以提高电源系统运行的可靠性,但是由于各个电源单体效率特性的差异,致使电源系统在运行过程中的总体效率无法达到最优,造成了能源的浪费。以效率优化为目标的复合式负荷分配控制策略,在动态调节过程中,利用均流技术确保安全运行,在稳态运行时,通过对负荷分配方案的优化,可以实现电源系统的总体效率最优控制,进而提升电源系统的运行性能。

关键词：并联运行效率优化负荷分配优化算法

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大剂量氯氮平中毒抢救成功一例

中华劳动卫生职业病杂志 2019年第2期37卷 154-155页

作者：王彦昌德州市市立医院急诊科德州253000

氯氮平为二苯二氮杂[艹卓]类抗精神病药,不仅对精神病阳性症状有效,对阴性症状也有一定效果。大剂量服用可以导致昏迷、谵妄、心动过速、低血压、呼吸衰竭、唾液分泌过多、癫痫等症状,甚至死亡。我们成功救治1例口服约250片氯氮平中毒患... 详细信息

氯氮平为二苯二氮杂[艹卓]类抗精神病药,不仅对精神病阳性症状有效,对阴性症状也有一定效果。大剂量服用可以导致昏迷、谵妄、心动过速、低血压、呼吸衰竭、唾液分泌过多、癫痫等症状,甚至死亡。我们成功救治1例口服约250片氯氮平中毒患者,现报道如下。

关键词：氯氮平大剂量中毒抢救抗精神病药阳性症状阴性症状心动过速呼吸衰竭

Microfacies and diagenetic alteration in a semi-deep to deep lacustrine shale: The yanchang Formation in the Ordos Basin, China

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Petroleum Science 2024年第3期21卷 1524-1538页

作者： Bin Bai Jing-Yue Hao Ling Fu Yu-Xi Liu Jian-Peng wang Lan wang Kevin GTaylor Lin Ma PetroChina Research Institute of Petroleum Exploration and Development Beijing100083China Department of Earth and Environmental Sciences University of ManchesterM139PLUK College of Earth Sciences Jilin UniversityChangchun130061jilinChina Department of Chemical Engineering University of ManchesterM139PLUK

The mineralogical development and diagenetic sequence of lacustrine shales in the Chang 7 Member of the yanchang Formation in the Ordos Basin are detailed studied.A model of their depositional system and a diagenetic diagram are proposed in this *** detailed petrographic,mineralogical,and elemental analyses,four distinct shale types are identified:argillaceous shale,siliceous shale,calcareous shale,and carbonate,clay,and silt-bearing *** main diagenetic process in argillaceous shale is the transformation of illite to smectite,negatively impacting shale *** shale undergoes carbonate cementation and quartz dissolution,contributing to increased porosity,particularly in *** shale experiences diagenesis characterised by carbonate formation and dissolution,with a prevalence of *** carbonate,clay,and silt-bearing shale,the dissolution of K-feldspar contributes to illitization of *** shale,characterised by more clay minerals and lower mesopore volume,is identified as a potential hydrocarbon *** shale,with the highest pore volume and abundant inter-mineral pores,emerges as a promising shale oil *** findings contribute to a comprehensive understanding of shale properties,aiding in the prediction of shale oil exploration potential in the studied area.

关键词： Ordos Basin yanchang Formation Chang 7 Member Diagenesis Microfacies

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医药分业:近代药界的身份建构与医药关系

近代史研究 2024年第3期 112-129,M0005,M0006页

作者：李彦昌北京大学医学人文学院

近代以来,西医药传入中国,不仅引发医药的中西之争,还引起医与药关系的重构.随着药界的壮大与觉醒,医药分业在知识、学科、职业、制度等多领域展开,逐渐成为药界竞争知识资本、文化资本、经济资本与政治资本的手段与路径,并推动了药物... 详细信息

近代以来,西医药传入中国,不仅引发医药的中西之争,还引起医与药关系的重构.随着药界的壮大与觉醒,医药分业在知识、学科、职业、制度等多领域展开,逐渐成为药界竞争知识资本、文化资本、经济资本与政治资本的手段与路径,并推动了药物科学化、药科建制化、药界职业化、药政制度化的进程,促进了药界的自我意识觉醒和身份建构、制度建设.但是,医药分业是各种因素综合导致的结果,并不必然促进药学转型、药科发展、药业振兴和药制进步.

关键词：医药分业医药关系药界药学社会史

TFT-IGZO工艺制程中TFT特性研究与改善

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光电子技术 2023年第2期43卷 173-176页

作者：赵辉华涛王艳昌南京京东方显示技术有限公司南京210033

分析了IGZO-TFT器件的基本特性及电性不稳定性影响因素。对有源保护层薄膜性能与TFT电学特性的依存关系给出了合理解释,并通过实验验证优化了有源保护层制备手法,解决了因有源保护层SiO2致密性引起的TFT开关阈值电压左向偏移显示不良问... 详细信息

分析了IGZO-TFT器件的基本特性及电性不稳定性影响因素。对有源保护层薄膜性能与TFT电学特性的依存关系给出了合理解释,并通过实验验证优化了有源保护层制备手法,解决了因有源保护层SiO2致密性引起的TFT开关阈值电压左向偏移显示不良问题;同时还基于IGZO-TFT总结了PECVD SiO2薄膜特性与沉积各重要因素间的关系。

关键词：薄膜晶体管铟镓锌氧化物电流电压特性曲线二氧化硅薄膜显示不良