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Science China Chemistry 2024年第7期67卷 2240-2247页

作者： Gaopan Liu yanbing Mo Jiawei Chen Yu Peng Xiao zhu Fei Wang Xiaoli Dong Yongyao Xia Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials Institute of New EnergyLaboratory of Advanced MaterialsFudan UniversityShanghai 200438China

Common evaluation methodology of sodium(Na)-containing two-electrode or three-electrode configurations overlooks the interference from highly reactive Na metal,leading to the underestimation or inconsistent performance of low-potential hard carbon(HC)electrodes.Herein,the trap of Na metal was systematically investigated with or without applied current,uncovering its inadequacy as the reference or counter electrode in different configurations.A Na-metal-free three-electrode protocol is proposed for evaluating the actual Na^(+)-storage capability of the typical low-potential HC electrode.By avoiding Na crosstalk and precisely controlling the working electrode's potential,the actual electrochemical performance of HC in the carbonate esterbased electrolyte can be recognized with high capacity of 222 mAh g^(-1)at 2 C and 113 mAh g^(-1)at 5 C,correcting the misunderstanding of the inferior performance of HC in coin-type half cells(68%and 50%undervaluation at 2 C and 5 C,respectively).The advanced protocol is expected to reduce misunderstandings or underestimation due to evaluation methods and to guide the development of high-performance battery materials.

关键词： highly reactive sodium potential drift carbonate ester sodium-metal-free protocol sodium storage capability

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卡夫卡小说《美国》的英译研究

山东外语教学 2024年第3期45卷 11-21页

作者：朱燕秋曾艳兵贵州民族大学外国语学院贵州贵阳550025 中国人民大学文学院北京100872

《美国》是弗兰兹·卡夫卡的第一部长篇小说,也是其被翻译得最多的作品之一。卡夫卡逝世百年来,《美国》的英译大致历经了发轫期、校勘期和繁荣期三个阶段。由于译者翻译动机和文化语境的不同,每个阶段的译本呈现出鲜明的差异性,在不同... 详细信息

《美国》是弗兰兹·卡夫卡的第一部长篇小说,也是其被翻译得最多的作品之一。卡夫卡逝世百年来,《美国》的英译大致历经了发轫期、校勘期和繁荣期三个阶段。由于译者翻译动机和文化语境的不同,每个阶段的译本呈现出鲜明的差异性,在不同程度上助推了“卡夫卡式”和“卡夫卡热”的兴起与传播,刺激了全球范围内的卡夫卡研究。深入考察《美国》英语译介的脉络,不仅能多维地呈现作品百年间的英译图景,也有助于理解卡夫卡作品在英语世界的传播、接受和经典化进程。

关键词：卡夫卡《美国》缪尔夫妇迈克尔·霍夫曼马克·哈曼里奇·罗伯逊

Propofol Attenuates a-Synuclein Aggregation and Neuronal Damage in a Mouse Model of Ischemic Stroke

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Neuroscience Bulletin 2020年第3期36卷 289-298页

作者： Yuzhu Wang Dan Tian Changwei Wei Victoria Cui Huan Wang yanbing zhu Anshi Wu Yun Yue Department of Anesthesiology Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China Experimental and Translational Research Center Beijing Friendship HospitalCapital Medical UniversityBeijing 100050China Washington University School of Medicine St.LouisMIUSA

a-Synuclein is a soluble monomer abundant in the central nervous system. Aggregates of a-synuclein,consisting of higher-level oligomers and insoluble fibrils,have been observed in many chronic neurological diseases and are implicated in neurotoxicity and neurodegeneration.a-Synuclein has recently been shown to aggregate following acute ischemic stroke, exacerbating neuronal damage.Propofol is an intravenous anesthetic that is commonly used during intravascular embolectomy following acute ischemic stroke. While propofol has demonstrated neuroprotective properties following brain injury, the mechanism of protection in the setting of ischemic stroke is unclear. In this study, propofol administration significantly reduced the neurotoxic aggregation of a-synuclein, decreased the infarct area, and attenuated the neurological deficits after ischemic stroke in a mouse model. We then demonstrated that the propofol-induced reduction of a-synuclein aggregation was associated with increased mammalian target of rapamycin/ribosomal protein S6 kinase beta-1 signaling pathway activity and reduction of the excessive autophagy occurring after acute ischemic stroke.

关键词： Propofol a-Synuclein Autophagy Stroke Neuroprotection

Overexpression and characterization of a thermostable β-agarase producing neoagarotetraose from a marine isolate Microbulbifer sp.AG1

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Acta Oceanologica Sinica 2019年第2期38卷 96-106页

作者： yanbing zhu He Gao Hebin Li Hui Ni Zedong Jiang Lijun Li Anfeng Xiao College of Food and Biological Engineering Jimei University Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering Research Center of Food Biotechnology of Xiamen City Key Laboratory of Systemic Utilization and In-depth Processing of Economic Seaweed Xiamen Southern Ocean Technology Center of China Department of Pharmacy Xiamen Medical College

An agarase gene containing 1 302 bp was cloned from Microbulbifer sp. AG1. It encoded a mature protein of 413 amino acids plus a 20-residue signal peptide. The recombinant enzyme without the signal peptide was expressed and purified from Escherichia coli BL21(DE3). When agarose was used as a substrate, the optimal temperature and pH for the enzyme were 60℃ and 7.5, respectively. The recombinant agarase showed excellent thermostability with 67% and 19% of residual activities after incubation at 50℃ and 60℃ for 1 h, respectively.Except SDS, the recombinant agarase had a relatively good resistance against the detected inhibitors, detergents and urea denaturant. Thin layer chromatography analysis and enzyme assay using p-nitrophenyl-α/β-Dgalactopyranoside revealed that the recombinant agarase was a β-agarase that degraded agarose into neoagarotetraose as the main end product. The enzymatic hydrolysis products with different degree of polymerization exhibited the antioxidant activities.

关键词： thermostable β-agarase neoagarotetraose Microbulbifer sp.

Propofol Protects Regulatory T Cells, Suppresses Neurotoxic Astrogliosis, and Potentiates Neurological Recovery After Ischemic Stroke

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Neuroscience Bulletin 2021年第5期37卷 725-728页

作者： Yuzhu Wang Dan Tian Yushang Zhao Mengyao Qu Yuhualei Pan Changwei Wei yanbing zhu Anshi Wu Department of Anesthesiology Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China Experimental and Translational Research Center Beijing Friendship HospitalCapital Medical UniversityBeijing 100050China

Dear Editor,Emergency intravascular thrombectomy is the best treatment for acute ischemic stroke [1]. However, it is controversial whether the use of general anesthesia during emergency intravascular thrombectomy affe... 详细信息

关键词： anesthesia acute intravascular

4种红藻的体外模拟消化酵解特性及其抗炎作用

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中国食品学报 2024年第4期24卷 118-129页

作者：周怡倩郑明静洪涛朱艳冰倪辉姜泽东集美大学海洋食品与生物工程学院福建厦门361021 福建省食品微生物学与酶工程重点实验室福建厦门361021 厦门市食品生物技术研究中心福建厦门361021

目的:通过体外模拟消化酵解和细胞炎症模型探究江蓠、红毛藻、麒麟菜和坛紫菜等4种经济红藻的消化酵解特性及其抗炎潜力,为红藻的高附加值开发提供依据。方法:通过体外模拟口胃肠消化和结肠发酵,测定4种红藻中总糖、还原糖、蛋白质和总... 详细信息

目的:通过体外模拟消化酵解和细胞炎症模型探究江蓠、红毛藻、麒麟菜和坛紫菜等4种经济红藻的消化酵解特性及其抗炎潜力,为红藻的高附加值开发提供依据。方法:通过体外模拟口胃肠消化和结肠发酵,测定4种红藻中总糖、还原糖、蛋白质和总酚的去向。利用16S r RNA基因Illumina MiSeq测序分析红藻对肠道菌群的影响,同时通过高效液相色谱法分析发酵产物中短链脂肪酸的含量,并采用脂多糖诱导的RAW264.7细胞炎症模型评价4种红藻发酵产物的抗炎活性。结果:在口胃肠消化过程中,坛紫菜对碳水化合物、蛋白质及酚类物质具有较好的生物利用度。难以消化的部分到达结肠后充当肠道微生物群的能量来源,其中坛紫菜在发酵过程中产生更多的短链脂肪酸。同时,4种红藻均表现出调节肠道菌群的能力,包括提高拟杆菌门的丰度,降低厚壁菌门/拟杆菌门的比值。此外,红藻发酵液对脂多糖诱导的RAW264.7细胞炎症损伤具有显著抑制作用,红毛藻发酵液具有更好的抑制效果。结论:4种红藻经模拟消化后有较好的生物可及性,能够调节肠道微生物群,同时表现出较好的抗炎潜力,为基于红藻的高值活性产品的开发提供科学依据。

关键词：红藻模拟消化模拟酵解肠道菌群抗炎

Substitution of His260 residue alters the thermostability of Pseudoalteromonas carrageenovora arylsulfatase

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Acta Oceanologica Sinica 2019年第6期38卷 75-82页

作者： yanbing zhu Xiaoqian Yin Han Liu Hebin Li Yanhong Chen Lijun Li Anfeng Xiao Hui Ni College of Food and Biological Engineering Jimei UniversityXiamen 361021China Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering Xiamen 361021China Research Center of Food Biotechnology of Xiamen City Xiamen 361021China Key Laboratory of Systemic Utilization and In-depth Processing of Economic Seaweed Xiamen Southern Ocean Technology Center of ChinaXiamen 361021China Department of Pharmacy Xiamen Medical CollegeXiamen 361023China

This study aimed to improve the thermostability of arylsulfatase from Pseudoalteromonas carrageenovora. A library of P. carrageenovora arylsulfatase mutants was constructed by introducing random mutagenesis using error-prone PCR. After screening, two mutants of H260L and D84A/H260L showed enhanced thermal stability than the wild-type predecessor (WT). Site-directed mutagenesis demonstrated that only amino acid residue at Position 260 plays an important role in the thermostability of P. carrageenovora arylsulfatase. Thermal inactivation analysis showed that the half-life (t1/2) values at 55°C for H260L, H260I, H260Q, H260F and H260R were 40.6, 48.4, 30.9, 29.1 and 34.5 min, respectively, while that of WT was 9.1 min. Structure modeling demonstrated that the additional hydrogen bonds and/or optimization of surface charge-charge interactions could be responsible for the increased thermostability imparted by H260L, H260I, H260Q, H260F and H260R.

关键词： arylsulfatase Pseudoalteromonas carrageenovora directed evolution error-prone PCR thermostability

船用柴油机涡轮辅助EGR系统多参数优化匹配研究

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船舶工程 2024年第2期46卷 64-72页

作者：王广夫祝彦兵张子悦张琨汤旭阳石磊海装沈阳局驻大连地区第一军事代表室辽宁大连116001 上海船舶设备研究所上海200031 上海中船重工船舶推进设备有限公司上海200031 上海交通大学动力机械及工程教育部重点实验室上海200240

为获得柴油机、排气再循环(EGR)系统和涡轮增压系统的耦合优化匹配运行规律,以SC7H两级增压柴油机为研究对象,在GT-Power软件中搭建并校核原机一维模型,并进一步建立了涡轮辅助EGR两级增压柴油机一维模型。针对涡轮辅助EGR与两级增压耦... 详细信息

为获得柴油机、排气再循环(EGR)系统和涡轮增压系统的耦合优化匹配运行规律,以SC7H两级增压柴油机为研究对象,在GT-Power软件中搭建并校核原机一维模型,并进一步建立了涡轮辅助EGR两级增压柴油机一维模型。针对涡轮辅助EGR与两级增压耦合系统的匹配问题,提出了基于过量空气系数λ和EGR率需求的三步骤匹配计算流程。匹配规律显示:高/EGR涡轮匹配流量比和高/低压级压比比基本呈线性关系,涡轮辅助EGR进排气压差随压比比增大先增加后减小,在压比比为1附近取最优,比高压EGR高35.35 kPa,涡轮辅助EGR具有更优的泵气过程。建立全连接神经网络优化代理模型并采用NSGAⅡ算法开展耦合系统多参数多目标优化研究。结果显示:涡轮辅助EGR具有更优的BSFC和NOx排放效果,在TierⅢ的加权NOx排放限值条件下,涡轮辅助EGR可调两级增压系统的全负荷加权油耗比传统高压EGR可调两级增压系统低5.20 g/(kW·h)。

关键词：柴油机废气再循环两级增压匹配方法油耗与排放

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基于关节点连接广度矩阵的颈椎康复运动识别

软件导刊 2024年第1期23卷 32-41页

作者：朱子豪何宏汪焰兵孙浩上海理工大学健康科学与工程学院上海200093

为提高颈椎康复动作识别的准确率与实时性,提出一种基于关节点连接广度矩阵的颈椎康复运动识别方法。在预处理阶段,首先从人体实时视频流数据中进行骨架提取;然后根据人体骨骼运动特点准确提取运动时人体骨架的特征信息,获取局部空间上... 详细信息

为提高颈椎康复动作识别的准确率与实时性,提出一种基于关节点连接广度矩阵的颈椎康复运动识别方法。在预处理阶段,首先从人体实时视频流数据中进行骨架提取;然后根据人体骨骼运动特点准确提取运动时人体骨架的特征信息,获取局部空间上的变化信息,利用广度优先搜索算法遍历骨架图内关节点,建立关节点连接广度矩阵,根据连接广度信息划分三角子图并为关节点分配权重,提高模型的识别效率;最后提取关节点连接广度矩阵的时空特征,通过SVM分类器完成识别。在颈椎康复运动数据集CRED和MSR Action 3D公开数据集上对该识别方法进行验证,实验结果表明,基于关节点连接广度矩阵的颈椎康复运动识别方法平均耗时为1.20 s,平均帧率为27,平均识别准确率为92.72%,与现有方法相比有一定优势。

关键词：颈椎康复运动关节点连接广度矩阵人体骨架三角子图时空特征

鄂尔多斯盆地五蛟西地区长6段单砂体叠置特征研究

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地质科学 2024年第4期59卷 1034-1047页

作者：武芳芳瞿璇周宁杰朱薇李彦兵周小平张东阁张庆军郭岭西北大学大陆动力学国家重点实验室西北大学地质学系中国石油长庆油田分公司长庆实业集团有限公司

沉积微相控制下的单砂体发育类型、频率和叠置样式，控制了储层的连通性和井间剩余油的分布。本次研究综合利用丰富的岩心、测井和分析测试资料，在沉积微相精细刻画基础上，对鄂尔多斯盆地五蛟西地区三叠系延长组长6段单砂体发育特征... 详细信息

沉积微相控制下的单砂体发育类型、频率和叠置样式，控制了储层的连通性和井间剩余油的分布。本次研究综合利用丰富的岩心、测井和分析测试资料，在沉积微相精细刻画基础上，对鄂尔多斯盆地五蛟西地区三叠系延长组长6段单砂体发育特征和叠置样式进行精细研究。结果表明：五蛟西地区长6段岩性以粉砂岩、细砂岩、含泥砾或泥岩撕裂屑的细砂岩和泥岩为主，发育沉积微相类型主要为砂质碎屑流、浊流和半深湖泥3种。长6段垂向上可识别出5～8期单砂体，单砂体叠置类型包括孤立型、叠加型以及切叠型3种；平面上单层砂体多以枝状、网状、局部片状展布。湖平面升降导致的可容纳空间（A）变化及沉积物供给速度（S）匹配关系是控制单砂体空间发育特征的关键性因素。研究区长6段自下而上，随着A/S比值的变化，单砂体类型垂向上由叠加型、切叠型逐渐向孤立型、局部叠加型变化，平面上由网状、枝状向局部片状演化。

关键词：单砂体重力流沉积叠置样式鄂尔多斯盆地五蛟西地区长6段