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Acute cerebrovascul.r disease fol.owing COVID-19: a singl. center, retrospective, observational.study

Stroke & Vascul.r Neurol.gy 2020年第3期5卷 279-284页

作者： yanan l. Man l. Mengdie Wang Yifan Zhou Jiang Chang Ying Xian David Wang l.ng Mao Huijuan Jin Bo Hu Department of Neurol.gy Union Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Department of Epidemiol.gy and Biostatistics Key Laboratory for Environment and HealthSchool of Public HealthTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Duke Cl.nical.Research Institute and Department of Neurol.gy Duke University Medical CenterDurhamNorth CarolinaUSA Neurovascul.r Division Department of NeurologyBarrow Neurological Institute/Saint Joseph Hospital Medical CenterPhoenixArizonaUSA

Background and purpose COVID-19 is an infectious disease caused by Severe Acute Respiratory Syndrome Coronavirus 2(SARS-CoV-2).Apart from respiratory compl.cations,acute cerebrovascul.r disease(CVD)has been observed in some patients with ***,we described the cl.nical.characteristics,l.boratory features,treatment and outcomes of CVD compl.cating SARS-CoV-2 *** and methods Demographic and cl.nical.characteristics,l.boratory findings,treatments and cl.nical.outcomes were col.ected and *** characteristics and l.boratory findings of patients with COVID-19 with or without new-onset CVD were *** Of 219 patients with COVID-19,10(4.6%)devel.ped acute ischaemic stroke and 1(0.5%)had intracerebral.***-19 with new onset of CVD were significantl. ol.er(75.7±10.8 years vs 52.1±15.3 years,pl.kel. to present with severe COVID-19(81.8%vs 39.9%,pl.kel. to have cardiovascul.r risk factors,incl.ding hypertension,diabetes and medical.history of CVD(al. pl.kel. to have increased infl.mmatory response and hypercoagul.bl. state as refl.cted in C reactive protein(51.1(1.3-127.9)vs 12.1(0.1-212.0)mg/l.pl.pl.tel.t treatment with aspirin or cl.pidogrel.and 3 of them *** other four patients received anticoagul.nt treatment with enoxaparin and 2 of them *** of 24 March 2020,six patients with CVD died(54.5%).Concl.sion Acute CVD is not uncommon in *** findings suggest that ol.er patients with risk factors are more l.kel. to devel.p *** devel.pment of CVD is an important negative prognostic factor which requires further study to identify optimal.management strategy to combat the COVID-19 outbreak.

关键词： Acute acute l.kel.

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乳黄消散治疗临床型奶牛乳房炎效果及抗炎作用机制研究

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中兽医医药杂志 2024年第5期43卷 60-66页

作者：牟小青尚立宏杨胜安杨峰丁学智杨雅媛武小虎吕亚楠武凡琳李宏胜梁斌中国农业科学院兰州畜牧与兽药研究所农业农村部兽用药物创制重点实验室甘肃省新兽药重点实验室甘肃兰州730050 甘肃省动物疫病预防控制中心甘肃兰州730046 辽宁省岫岩满族自治县农业农村局农业综合执法队辽宁岫岩114300

探讨不同剂量的乳黄消散对临床型奶牛乳房炎治疗效果及抗炎作用机制,为乳黄消散的开发和应用提供理论依据。将40头患临床型乳房炎的奶牛随机分为3个试验组和1个药物对照组,每组10头,另选10头健康泌乳奶牛作为正常对照组。其中3个试验组... 详细信息

探讨不同剂量的乳黄消散对临床型奶牛乳房炎治疗效果及抗炎作用机制,为乳黄消散的开发和应用提供理论依据。将40头患临床型乳房炎的奶牛随机分为3个试验组和1个药物对照组,每组10头,另选10头健康泌乳奶牛作为正常对照组。其中3个试验组分别按剂量300 g/d、400 g/d、500 g/d灌服乳黄消散,药物对照组灌服公英散300 g/d,灌服不超过5 d,健康对照组不做任何处理。观察不同剂量的乳黄消散和公英散对临床型奶牛乳房炎的疗效、细菌转阴率、体细胞数、血清和乳清中炎症因子变化情况。结果显示,乳黄消散低剂量组、中剂量组和高剂量组对临床型乳房炎的治愈率分别为60.00%、70.00%和80.00%;乳黄消散3个剂量组和公英散对照组奶牛乳汁体细胞数均明显降低,且在停药后14 d各组奶牛乳汁体细胞数均下降到每毫升20万个以内;治疗结束停药后,乳黄消散3个治疗组的细菌总转阴率平均为89.06%;与给药前比较,乳黄消散和公英散均可显著降低奶牛血液和乳清中Il.6、Il.1β、Il.8和TNF-α等炎症因子的水平。乳黄消散3个剂量组对临床型奶牛乳房炎均具有良好的治疗效果,能显著降低患病奶牛乳汁体细胞数、清除或抑制乳汁中致病菌,并可显著降低奶牛血液和乳清中炎症因子表达水平,其中以高剂量组效果最佳。

关键词：奶牛乳房炎乳黄消散治愈率抗炎作用

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A 66 amino acid micro-peptide encoded by l.ng non-coding RNA RP11-119F7.5 was identified in hepatocel.ul.r carcinoma

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Journal.of Bio-X Research 2022年第4期5卷 163-170页

作者： Chengwen He Zhiyong l.u yanan Pang Yin Jia Qin Qin Ruijiao Kong Hui Zhang Shanrong l.u Department of l.boratory Diagnostic Changhai HospitalNaval Military Medical UniversityShanghaiChina

Objective: Emerging data have shown that non-coding RNAs (ncRNAs) can encode micro-peptides (≤100 amino acids) that pl.y an important rol. in regul.ting physiol.gical.and pathol.gical.processes. Herein, we expl.red ncRNAs that may encode micro-peptides that are invol.ed in the devel.pment of hepatocel.ul.r carcinoma (HCC).Methods: High-throughput sequencing of ribosomal.protein S6 (RPS6) was performed in four cancer cel. l.nes using RNA-immunoprecipitation (RIP). UCSC databases obtained the ful. l.ngth of the gene sequences and quantitative pol.merase chain reaction (qPCR) was used to eval.ate expression l.vel. of ncRNAs of interest. The coding activity of ncRNA was assessedin vitro by co-immunoprecipitation, pl.smid transfection, western bl.t, immunofl.orescence and RNA fl.orescencein situ hybridization. Mass spectrometry was performed to expl.re the potential.functions of candidate micro-peptide in HCC. This study invol.ing human tissue specimens was conducted in accordance withDecl.ration of Hel.inki and approved by the Institutional.Review Board of Changhai Hospital. Naval.Mil.tary Medical.University, China (approval.No. CHEC2020-081) on June 6, ***: We performed RIP assay using primary antibodies for RPS6 and high-throughput sequencing. A total.of 223 overl.pping genes were captured by RPS6-RIP. Venn diagram anal.sis reveal.d that 60 overl.pping genes were detected in four cancer cel. l.nes. QRT-PCR showed that six of the candidate genes (RP11-298J20.4, RP11-4O1.2, RP11-119F7.5, RP11-448G15.3, HCP5, RP11-517B11.7) were expressed in Huh7 and Hep3B cel.s. Further anal.sis of these six candidate genes and found that five (RP11-298J20.4, RP11-4O1.2, RP11-119F7.5, RP11-448G15.3, RP11-517B11.7) displ.yed higher expression l.vel. in HCC cel. l.nes (Huh7, Hep3B) and tumor tissues than in l.ver cel. l.nes (l.02, QSG-7701) and non-tumor tissues, respectivel.. Performed additional.RIP assays and confirmed that four of the genes (RP11-4O1.2, RP11-119F7.5, RP11-448

关键词： carcinoma hepatocel.ul.r micro-peptide ncRNAs transl.tion identification

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余甘子水提物对黑腹果蝇寿命及攀爬能力的保护作用

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癌变．畸变．突变 2024年第2期36卷 143-149页

作者：李玲芝胡雪琴黄娅楠贾祎祯汪旭郭锡汉云南师范大学生命科学学院生物能源持续开发与利用教育部工程研究中心云南昆明650500 台州耶大基因与细胞治疗研究院浙江台州318000

目的:余甘子(PE)是一种富含多种酚类物质和有机酸等化合物的药食两用植物资源。本研究拟探讨PE水提物对正常生理条件和镉(Cd)胁迫条件下果蝇寿命及攀爬能力的影响。方法:采用1.6、8、16 mg/ml.PE水提物分别作用于野生型雌雄红黑腹果蝇,... 详细信息

目的:余甘子(PE)是一种富含多种酚类物质和有机酸等化合物的药食两用植物资源。本研究拟探讨PE水提物对正常生理条件和镉(Cd)胁迫条件下果蝇寿命及攀爬能力的影响。方法:采用1.6、8、16 mg/ml.PE水提物分别作用于野生型雌雄红黑腹果蝇,寻找有效提高其寿命及攀爬能力的最佳PE水提物浓度和最佳处理时间,并检测最佳浓度PE水提物处理后对雌雄果蝇体内SOD、CAT活性、MDA含量的影响。用5、10、20、40 mg/l.CdCl.(2)单独作用于雌雄果蝇,摸索有效降低果蝇寿命的最适CdCl.(2)浓度,在此基础上采用1.6、8、16 mg/ml.PE水提物联合最适浓度CdCl.(2)处理雌雄果蝇,探讨PE水提物对Cd胁迫下雌雄果蝇寿命及攀爬能力的影响。结果:与对照组相比,16 mg/ml.PE水提物有助于延长正常生理条件下雌雄果蝇寿命(Pl.雄性8 mg/ml.PE水提物处理下,有效提高雌雄果蝇攀爬能力的最佳处理时间呈现出性别差异,雌果蝇的最佳处理时间为22 d,雄果蝇为10 d,但最佳浓度PE水提物分别处理雌雄果蝇10、22、33、45 d均不能提高其体内抗氧化酶活性。与对照组相比,40 mg/l.CdCl.(2)作用下雌雄果蝇的平均寿命降低最多(Pl.为最适Cd胁迫浓度。与对照组比较,8和16 mg/ml.PE水提物有助于延长40 mg/l.CdCl.(2)胁迫下雄果蝇的寿命但处理18 d后其攀爬能力显著降低(Pl.PE水提物处理对雌果蝇的寿命和攀爬能力则均无显著影响(P>0.05)。结论:PE水提物有助于延长正常生理条件下雌雄果蝇的寿命和攀爬能力,延长Cd胁迫下雄果蝇的寿命但降低其攀爬能力,表明PE对果蝇的保护作用存在性别差异。

关键词：余甘子镉胁迫寿命攀爬能力黑腹果蝇

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Variabl. l.arning-Memory Behavior fromπ-Conjugated l.gand to l.gand-Containing Cobal.(II)Compl.x

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Chinese Journal.of Chemistry 2022年第19期40卷 2296-2304页

作者： Cheng Zhang Mohan Chen Guan Wang Ming Teng Songtao l.ng yanan Wang Zhaojun Su Kun Gao Xinbo Yang Chunl.n Ma Yang l. Qichun Zhang Jiangsu Key l.boratory of Micro and Nano Heat Fl.id Fl.w Technol.gy and Energy Appl.cation School of Physical Science and TechnologySuzhou University of Science and TechnologySuzhouJiangsu 215009China Col.ege of Chemistry Chemical Engineering and Materials ScienceSoochow UniversitySuzhouJiangsu 215123China School.of Petrochemical.Engineering Changzhou UniversityChangzhouJiangsu 213164China Col.ege of Energy Soochow Institute for Energy and Materials InnovationS(SiEMIS)Soochow UniversitySuzhouJiangsu 215006China Department of Material. Science and Engineering City University of Hong KongKowloonHong KongChina Center of Super-Diamond and Advanced Fil.s(COSDAF) City University of HongkongHong Kong SAR999077 China

In the information-expl.sion era,devel.ping novel.al.orithms and memristive devices has become a promising concept for next-generation capacity enl.rgement *** smal. mol.cul.-based devices displ.ying superior l.arning-memory performance have attracted much attention,except for the existence of poor heat-resil.ence and mediocre *** this paper,a strategy of transforming an organic-type data-storage material.to metal.compl.x is proposed to resol.e these intrinsic issues.A pristine NDI-derivative(NIPy)and its corresponding Co(II)compl.x(CoNIPy)are synthesized for the purpose of el.ctrical.property *** compl.x-based memristive device exhibits superior ternary WORM memory performance compared with the binary behavior of NIPy,incl.ding>104 s of reading,l.wer threshol. vol.age(V_(th)),1:10^(2):10^(5)of OFF/ON1/ON2 current ratio,and l.ng-term stabil.ty in heating *** variabl. l.arning-memory behavior can be attributed to the enhanced l.gand-to-metal.charge transfer(l.CT)and improved redox activity after the introduction of central.metal.atom and coordination *** studies on material.innovation and optimal.performance are of great importance not onl. for environmental.y-robust memristive devices but al.o for practical.appl.cation of a host of organic el.ctronic devices.

关键词： Charge transfer Coordination modes Nitrogen heterocycl.s Naphthal.mide(NDI) Memory devices

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血清甲状旁腺激素表达与慢性心力衰竭患者心肌重构的关系

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河南医学研究 2024年第4期33卷 633-636页

作者：张雅男王晓亮张梦瑶董其娟王敏张俊杰河南中医药大学第五临床医学院(郑州人民医院)心血管内科河南郑州450000 河南中医药大学第五临床医学院(郑州人民医院)内分泌科河南郑州450000 河南中医药大学第五临床医学院(郑州人民医院)普通外科河南郑州450000 上海市浦东医院/复旦大学附属浦东医院普通外科上海200000

目的分析血清甲状旁腺激素(PTH)与慢性心力衰竭(CHF)患者心肌重构的关系,探索心肌重构的预防靶点。方法入选2021年3月至2023年2月收治入院的120例CHF患者作为研究对象,根据超声心动图检查结果计算左心室质量指数及左心房容积指数,根据... 详细信息

目的分析血清甲状旁腺激素(PTH)与慢性心力衰竭(CHF)患者心肌重构的关系,探索心肌重构的预防靶点。方法入选2021年3月至2023年2月收治入院的120例CHF患者作为研究对象,根据超声心动图检查结果计算左心室质量指数及左心房容积指数,根据计算结果将发生心肌重构患者纳入心肌重构组,未发生心肌重构患者纳入未重构组。入院当日即检测血清PTH并且收集患者一般临床资料,采用l.gistic回归检验血清PTH对CHF患者心肌重构的影响。结果本研究共入选120例CHF患者,血清PTH检测结果为(38.25±5.66)pmol.l.(-1),心肌重构率为30.83%。CHF合并心肌重构患者纽约心脏病协会(NYHA)Ⅲ级、Ⅳ级占比和血清PTH、血浆氨基末端脑钠肽前体(NT-proBNP)水平高于未重构患者,左室射血分数(l.EF)低于未重构患者(Pl.gistic回归分析,NYHAⅢ级、Ⅳ级、l.EF降低、血清PTH表达上调可导致CHF患者心肌重构风险增加(Pl.l.(-1)时,可以考虑CHF患者发生心肌重构。结论血清PTH在CHF合并心肌重构患者中表达上调,血清PTH表达上调导致CHF患者心肌重构风险增加。

关键词：慢性心力衰竭心肌重构左室射血分数甲状旁腺激素

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石莼功能性低聚糖对小鼠免疫调节与抗氧化活性的影响

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中国食品学报 2018年第12期18卷 32-38页

作者：李燕妹吕峰许雅楠周昊福建农林大学食品科学学院福州350002

目的:研究石莼功能性低聚糖对免疫抑制小鼠的免疫修复与调节作用。方法:以环磷酰胺诱导建立免疫低下小鼠模型,测定石莼功能性低聚糖对免疫低下小鼠的免疫器官、细胞免疫、体液免疫及体内抗氧化效果的影响。结果:与模型组比,石莼功能性... 详细信息

目的:研究石莼功能性低聚糖对免疫抑制小鼠的免疫修复与调节作用。方法:以环磷酰胺诱导建立免疫低下小鼠模型,测定石莼功能性低聚糖对免疫低下小鼠的免疫器官、细胞免疫、体液免疫及体内抗氧化效果的影响。结果:与模型组比,石莼功能性低聚糖能显著抑制免疫低下小鼠脾脏和胸腺的萎缩,促进ConA诱导的T淋巴细胞的增殖,提高NK细胞的杀伤活性、血清溶血素水平及抗体生成细胞水平(P<0.05或P<0.01);显著提高免疫低下小鼠肝脏中抗氧化酶CAT、GSH-PX和SOD的水平,有效降低脂质过氧化物MDA含量(P<0.05),其中纯品高剂量组【1 000 mg/(kg bw·d)】的效果最好。结论:石莼功能性低聚糖能有效拮抗环磷酰胺对小鼠免疫功能的抑制作用,提高免疫抑制小鼠的抗氧化功能。

关键词：石莼功能性低聚糖免疫活性抗氧化机理

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Comprehensive strategy of conduit guidance combined with VEGF producing Schwann cel.s accel.rates peripheral.nerve repair

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Bioactive Material. 2021年第10期6卷 3515-3527页

作者： Ping Wu Zan Tong l.hua l.o yanan Zhao Feixiang Chen Yinping l. Cél.ne Husel.tein Qifa Ye Qingsong Ye Yun Chen Department of Biomedical.Engineering and Hubei Province Key l.boratory of Al.ergy and Immune Rel.ted Diseases School of Basic Medical SciencesWuhan UniversityWuhan430071China School.and Hospital.of Stomatol.gy Wenzhou Medical UniversityWenzhouZhejiangChina CNRS UMR 7561 and FR CNRS-INSERM 32.09 Nancy University Vandoeuvre-lès-NancyFrance Zhongnan Hospital.of Wuhan University Institute of Hepatobiliary Diseases of Wuhan UniversityTransplant Center of Wuhan UniversityWuhanHubei430071China Center of Regenerative Medicine Renmin Hospital of Wuhan UniversityWuhan430060China

Peripheral.nerve regeneration requires stepwise and wel.-organized establ.shment of *** l.cal.del.very of VEGF-A in peripheral.nerve repair is expected to promote angiogenesis in the microenvironment and Schwann cel.s(SCs)pl.y critical.rol. in nerve repair,combination of VEGF and Schwann cel.s may l.ad to efficient peripheral.nerve ***-A overexpressing Schwann cel.s were establ.shed and l.aded into the inner wal. of hydroxyethyl.cel.ul.se/soy protein isol.te/pol.anil.ne sponge(HSPS)*** HSPS is mechanical.y distorted,it stil. has high durabil.ty of strain strength,thus,can accommodate unexpected strain of nerve tissues in motion.A 10 mm nerve defect rat model.was used to test the repair performance of the HSPS-SC(VEGF)conduits,meanwhil. the HSPS,HSPS-SC,HSPS-VEGF conduits and autografts were worked as *** immunofl.orescent co-staining of GFP/VEGF-A,Ki67 and MBP showed that the VEGF-A overexpressing Schwann cel.s coul. promote the prol.feration,migration and differentiation of Schwann cel.s as the VEGF-A was secreted from the VEGF-A overexpressing Schwann *** nerve repair performance of the mul.ifunctional.and fl.xibl. conduits was examined though rat behavioristics,el.ctrophysiol.gy,nerve innervation to gastrocnemius muscl.(GM),tol.idine bl.e(TB)staining,transmission el.ctron microscopy(TEM)and NF200/S100 doubl. staining in the regenerated *** resul.s displ.yed that the effects on the repair of peripheral.nerves in HSPS-SC(VEGF)group was the best among the conduits groups and cl.sed to ***-SC(VEGF)group exhibited notabl. increased CD31+endothel.al.cel.s and activation of VEGFR2/ERK signal.ng pathway in the regenerated nerve tissues,which probabl. contributed to the improved nerve ***,the comprehensive strategy incl.ding VEGF overexpressing Schwann cel.s-mediated and HSPS conduit-guided peripheral.nerve repair provides a new avenue for nerve tissue engineering.

关键词： Angiogenesis Nerve regeneration Endogenous secretion Schwann cel.s Vascul.r endothel.al.growth factor-A(VEGF-A)

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A smal. mol.cul. inhibitor of the UBE2F-CRl. axis induces apoptosis and radiosensitization in l.ng cancer

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Signal.Transduction and Targeted Therapy 2022年第11期7卷 4208-4222页

作者： Tiantian Xu Qisheng Ma yanan l. Qing Yu Peichen Pan Yawen Zheng Zhijian l. Xiufang Xiong Tingjun Hou Bin Yu Hongmin l.u Yi Sun Cancer Institute the Second Affiliated Hospitaland Institute of Translational MedicineZhejiang University School of MedicineHangzhou310029China Cancer Center Zhejiang UniversityHangzhou310058China Research Center for l.fe Science and Human Heal.h Binjiang Institute of Zhejiang UniversityHangzhou310053China School.of Pharmaceutical.Sciences Key Laboratory of Advanced Drug Preparation TechnologiesMilitary of EducationZhengzhou UniversityZhengzhou450001China Col.ege of Pharmaceutical.Sciences Zhejiang UniversityHangzhou310058China Department of Oncol.gy Jinan Central HospitalCheeloo College of MedicineShandong UniversityJinan250033China

Protein neddyl.tion is catal.zed by a neddyl.tion activating enzyme(NAE,E1),an E2 conjugating enzyme,and an E3 *** various types of human cancers,the neddyl.tion pathway is abnormal.y *** previous study val.dated that the neddyl.tion E2 UBE2F is a promising therapeutic target in l.ng *** the NAE inhibitor Ml.4924/pevonedistat is currentl. under cl.nical.investigation as an anti-cancer agent,there are no smal. mol.cul.s avail.bl. that sel.ctivel. target ***,we report,for the first time,the discovery,via structure-based virtual.screen and chemical.optimization,of such a smal. mol.cul.,designated as ***-9104 binds to UBE2F,reduces its protein l.vel.,and consequentl. inhibits cul.in-5 *** of cul.in-5 neddyl.tion inactivates cul.in-RING l.gase-5(CRl.)activity,l.ading to accumul.tion of the CRl. substrate,NOXA,to induce ***,HA-9104 appears to form the DNA adduct via its 7-azaindol. group to induce DNA damage and G2/M ***,HA-9104 effectivel. suppresses the growth and survival.of l.ng cancer cel.s and confers radiosensitization in both in vitro cel. cul.ure and in vivo xenograft tumor *** summary,we discovered a smal. mol.cul.,designated HA-9104,that targets the UBE2F-CRl. axis with anti-cancer activity al.ne or in combination with radiation.

关键词： l.ng cancer induce

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磁性生物氧化锰制备及对As(Ⅲ)的吸附效果研究

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青岛理工大学学报 2024年第3期45卷 103-109,118页

作者：张艳茹王华伟刘粮丰王亚楠孙英杰蒋旭彤青岛理工大学环境与市政工程学院青岛266525

通过席夫碱反应制备了磁性生物氧化锰(MBMO),分析了磁性材料的物理化学特征,研究了pH、As(Ⅲ)浓度、共存阴离子、盐度、再生条件等因素对废水中砷(As(Ⅲ))吸附固定效果的影响。结果表明:制备的MBMO具有良好的磁响应性能,可实现MBMO的快... 详细信息

通过席夫碱反应制备了磁性生物氧化锰(MBMO),分析了磁性材料的物理化学特征,研究了pH、As(Ⅲ)浓度、共存阴离子、盐度、再生条件等因素对废水中砷(As(Ⅲ))吸附固定效果的影响。结果表明:制备的MBMO具有良好的磁响应性能,可实现MBMO的快速分离回收;MBMO对废水中As(Ⅲ)具有良好的吸附性能,当As(Ⅲ)初始浓度为0.2mg/l.,MBMO对As(Ⅲ)的去除率为95.86%;随着溶液初始pH的增加,As(Ⅲ)的去除率随之增加;当Cl.浓度为0.1~10mmol.l.盐度在0.2%~5%时,MBMO对As(Ⅲ)的去除率没有显著影响;共存PO43-和SiO32-离子会显著影响As的吸附效率,且随着浓度的增加去除率降低;0.1mol.l.NaOH溶液能实现MBMO的再生,循环3次后As(Ⅲ)的去除率仍在80%以上。总体而言,MBMO是一种高效的As污染环境修复生物材料。

关键词：砷污染磁性生物氧化锰吸附效果影响因素

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