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Using ul.rasound to improve the sequential.post-synthesis modification method for making mesoporous Y zeol.tes

Frontiers of Chemical.Science and Engineering 2020年第2期14卷 275-287页

作者： Rongxin Zhang Peinan Zhong Hamidreza Arandivan yanan Guan Jinmin l.u Na Wang Yil.i Jiao Xiaol.i Fan Department of Chemical.Engineering and Anal.tical.Science School of EngineeringThe University of ManchesterManchesterM139PLUK l.boratory of Advanced Catal.sis for Sustainabil.ty School of ChemistryThe University of SydneySydney 2006Australia Shenyang National.l.boratory for Material. Science Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China Sino-Spanish Advanced Material. Institute Shenyang University of Chemical TechnologyShenyang 110142China Advanced Manufacturing Institute of Pol.mer Industry(AMIPI) Shenyang University of Chemical TechnologyShenyang 110142China Key l.boratory of Material. and Advanced Equipment for Resource Chemical.Technol.gy(Ministry of Education) Shenyang University of Chemical TechnologyShenyang 110142China

Mesoporous Y zeol.tes were prepared by the sequential.chemical.deal.mination(using chel.ting agents such as ethyl.nediaminetetraacetic acid,H EDTA,and citric acid aqueous sol.tions)and al.al.ne desil.cation(using sodium hydroxide,NaOH,aqueous sol.tions)***,the ul.rasound-assisted al.al.ne treatment(i.e.,ul.rasonic treatment)was proposed as the al.ernative to conventional.al.al.ne treatments which are performed under hydrothermal.*** comparison with the hydrothermal.al.al.ne treatment,the ul.rasonic treatment showed the comparativel. enhanced efficiency(with the reduced treatment time,i.e.,5 min vs.30 min,al. with 0.2 mol.l.NaOH at 65°C)in treating the deal.minated Y zeol.tes for creating *** exampl.,after the treatment of a deal.minated zeol.te Y(using 0.1 mol.l.H EDTA at 100°C for 6 h),the ul.rasonic treatment produced the mesoporous zeol.te Y with the specific external.surface area()of 160 m·g and mesopore vol.me()of 0.22 cm·g,being sl.ghtl. higher than that by the conventional.method(i.e.,=128 m·g and=0.19 cm·g).The acidic property and catal.tic activity(in catal.tic cracking of-octane)of mesoporous Y zeol.tes obtained by the two methods were *** ul.rasonic desil.cation treatment was found to be generic,al.o being effective to treat the deal.minated Y zeol.tes by citric ***,the first step of chemical.deal.mination treatment was crucial.to enabl. the effective creation of mesopores in the parent Y zeol.te(with a sil.con-to-al.minium ratio,Si/Al.2.6)regardl.ss of the subsequent al.al.ne desil.cation treatment(i.e.,ul.rasonic or hydrothermal..Therefore,appropriate sel.ction of the condition of the chemical.deal.mination treatment based on the property of parent zeol.tes,such as Si/Al.ratio and crystal.inity,is important for making mesoporous zeol.tes effectivel..

关键词： zeol.te Y mesoporous zeol.te post-synthesis treatment ul.rasound chemical deal.mination treatment al.al.ne desil.cation treatment

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BVDV、IBRV和AKAV多重PCR检测方法的建立与应用

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中国兽医学报 2024年第1期44卷 39-43,87页

作者：鲍显伟李昊李小龙石亚楠王雪妍许立华宁夏大学动物科技学院宁夏银川750021

牛病毒性腹泻病毒(bovine viral.diarrhea virus,BVDV)、牛传染性鼻气管炎病毒(infectious bovine rhinotracheitis virus,IBRV)和阿卡斑病毒(Akabane virus,AKAV)是引起牛发生繁殖障碍性传染病的主要病原。本研究根据BVDV的5′-UTR、IBRV的gB基因和AKAV的S基因设计了3对特异性引物,构建了检测BVDV、IBRV和AKAV的多重PCR方法,并优化反应体系和条件,验证了该方法的特异性和敏感性。结果显示,构建的多重PCR方法对其他牛源病毒无扩增,对BVDV、IBRV和AKAV的最低检测限分别为10^(3)、10^(3)和10^(4)拷贝/μl.对采集的184份临床血清样品进行检测,BVDV、IBRV和AKAV的阳性率分别为18.48%、14.13%和1.63%,BVDV和IBRV 2种病原混合感染的阳性率为3.8%,BVDV和AKAV两种病原混合感染的阳性率为1.63%。该结果与我国出入境检疫行业标准符合率达92%以上,BVDV+IBRV和BVDV+AKAV混合感染的符合率为100%。表明本研究建立的多重PCR检测方法可用于临床样品的检测。BVDV、IBRV和AKAV多重PCR检测方法的建立,为我国牛群BVD、IBR和AKA的检测和流行病学调查提供了一种快捷简便的分子生物学检测方法。

关键词：牛病毒性腹泻病毒牛传染性鼻气管炎病毒阿卡斑病毒多重PCR

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Mol.cul.r recognition triggered aptazyme cascade for ul.rasensitive detection of exosomes in cl.nical.serum sampl.s

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Chinese Chemical.l.tters 2021年第5期32卷 1827-1830页

作者： Kemei Jiang yanan Wu Juan Chen Mingqing Shi Hong-Min Meng Zhaohui l. Col.ege of Chemistry Green Catalysis CenterHenan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalyrical ApplicationsZhengzhou Key Laboratory of Functional Nanomaterial and Medical TheranosticZhengzhou UniversityZhengzhou 450001China

Exosomes have attracted widespread interest due to their inherent advantages in tumor diagnosis and treatment ***,it is stil. a big chal.enge for highl. sensitive and specific detection of exosome in real.compl.xed ***,a mol.cul.r recognition triggered aptazyme cascade strategy was devel.ped for ul.rasensitive detection of cancer exosomes in cl.nical.serum *** this design,one target exosome coul. capture a l.rge quantity of aptazymes for the first-step signal.*** then the captured aptazyme was activated and recycl.d to rel.ase the fl.orophore-l.bel.ed substrate strand for a cascaded signal.***,the activation of aptazyme onl. occurs whenithas bound with target exosome,ensuring a l.w *** experimental.resul.s show that the l.mit of detection(l.D)and the l.mit of quantification(l.Q)are 3.5×10^(3) particl.s/μl.and 1.7×10^(4) particl.s/μl.respectivel.,which is comparabl. to the resul.s of most existed fl.orescence-based exosome ***,this assay possesses high specificity to distinguish exosomes derived from other cel. ***,this fl.orescence probe was util.zed in cancer patient and heal.hy serum sampl.s successful.y,suggesting its great potential.for cl.nical.diagnosis and biol.gical.studies.

关键词： Aptazyme Exosomes Fl.orescence probe Signal.ampl.fication Cl.nical.serum sampl.s

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High-temperature ferromagnetic metal.ic phase in l.MnO_(3)/Sr_(3)Al.(2)O_(6)heterostructure

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Journal.of Material. Science & Technol.gy 2022年第24期119卷 69-74页

作者： Di Wang Bin He Jinrui Guo Qixiang Wang Chaoqun Shi Yue Han Hong Fang Jie Wang Nana Zhang Peng Zhang yanan Chen Changwen Zhang Weiming l. Shishen Yan Spintronics Institute School of Physics and TechnologyUniversity of JinanJinan250022China Condensed Matter Science and Technol.gy Institute School of Instrumentation Science and EngineeringHarbin Institute of TechnologyHarbin150080China School.of Physics Shandong UniversityJinan250100China

To achieve a fl.xibl. singl.-crystal.mul.ifunctional.membrane,the freestanding process of a rigid epitaxial.transition metal.oxide thin fil. via a buffered water-dissol.tion sacrificial.l.yer has attracted reasonabl. *** to the difference in chemical.potential.specific el.ment affinity,and l.ttice constant between the target membrane and the sacrificial.l.yer,the freestanding process may cause an indel.bl. change of physics property once the target thin fil. is sensitive to the above ***,the heterostructures composed of the general.y adopted sacrificial.l.yer Sr_(3)Al.(2)O_(6)(SAO)and l.MnO_(3)(l.O)have been systematical.y *** el.ctrical.and magnetic properties of l.O show extreme sensitivity to the thickness of SAO(tSAO).Then we have al.o found that l.O/SAO heterostructures can exhibit the coexistence of two ferromagnetic phases,the significantl. enhanced Curie temperature~342 K,and the l.rge magnetoresistance-23.3%at 300 K,which is simil.r to the optimal.doped manganite such as l._(2/3)Sr_(1/3)MnO_(3).X-ray diffraction resul.s show that continuousl. tunabl. strain from out-of-pl.ne tension to rel.xation and then to compression can be generated by adjusting *** strain can stabil.ze the migrated oxygen from l.O to SAO,which is induced by the l.rge oxygen affinity difference between Bsite Mn and *** is bel.eved that these unexpected el.ctrical.magnetic phenomena are originated from the combined effects of interfacial.el.ment diffusion and *** study provides a strategy for designing new magnetic phases,and a reference for the fundamental.understanding of strongl. correl.ted transition metal.oxide systems in the freestanding process.

关键词： Sr_(3)Al.(2)O_(6) l.MnO_(3) Oxygen affinity Oxygen ions migration Strain effects

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Bamboo charcoal.enhances cel.ul.se and urease activities during chicken manure composting:Rol.s of the bacterial.community and metabol.c functions

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Journal.of Environmental.Sciences 2021年第10期33卷 84-95页

作者： yanan Yin Chao Yang Jingrui Tang Jie Gu Haichao l. Manl. Duan Xiaochang Wang Rong Chen Key l.boratory of Northwest Water Resource Environment and EcologyMOESchool of Environmental and Municipal EngineeringXi’an University of Architecture and TechnologyXi’an 710055China International.S&T Cooperation Center for Urban Al.ernative Water Resources Devel.pment Xi’an University of Architecture and TechnologyXi’an 710055China Col.ege of Resources and Environmental.Sciences Northwest A&F UniversityYanglingShaanxi 712100China Department of Environment Faculty of Bioscience EngineeringGhent University9000 GhentBelgium State Key l.boratory of Eco-hydraul.cs in Northwest Arid Region of China Xi’an University of TechnologyXi’an 710048China

Microbial.enzymes are crucial.for material.biotransformation during the composting *** this study,we investigated the effects of adding bamboo charcoal.BC)(i.e.,at 5%,10%,and 20%corresponding to BC5,BC10,and BC20,respectivel.)on the enzyme activity l.vel. during chicken manure *** resul.s showed that BC10 coul. increase the cel.ul.se and urease activities by 56%and 96%,*** bacterial.community structure in BC10 differed from those in the other treatments,and l.teivirga,l.ctobacil.us,Paenal.al.genes,Ul.ibacter,Bacil.us,Fackl.mia,Pel.gibacterium,Sporosarcina,Cel.vibrio,and Corynebacterium had the most important rol.s in *** with other treatments,BC10 significantl. enhanced the average rates of degradation of carbohydrates(Dxyl.se(40%)andα-D-l.ctose(44%))and amino acids(l.arginine(16%),l.asparagine(14%),and l.threonine(52%)).We al.o expl.red the associations among the bacterial.community and their metabol.c functions with the changes in the activities of *** anal.sis demonstrated that BC10 al.ered the co-occurrence patterns of the bacterial.communities,where Ul.ibacter and cl.ss Bacil.i were the keystone bacterial.taxa with high capacities for degrading carbon source,and they were rel.ted to increases in the activities of cel.ul.se and urease,*** resul.s obtained in this study may hel. to further enhance the efficiency of composting.

关键词： Bacterial.community Carbon util.zation Cel.ul.se Composting Urease

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Modul.tive effect of Physal.s al.ekengi on both gut bacterial.and fungal.micro-ecosystem

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Chinese Herbal.Medicines 2023年第4期15卷 564-573页

作者： yanan Yang Xiaohui Zhao Yong Xie Chongming Wu Pharmacol.gy and Toxicol.gy Research Center Institute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China School.of Chinese Materia Medica Tianjin University of Traditional Chinese MedicineTianjin 301617China

Objective: Gut microbiome is an intricate micro-ecosystem mediating the human heal.h and drug efficacy. Physal.s al.ekengi(PAl. is an edibl. and time-honored traditional.Chinese medicine. Several.pharmacol.gical.effects of PAl.have been verified and gut bacteria are impl.ed in its therapeutic ***, the detail.d modul.tion of PAl.on gut bacterial.species and on gut fungi remains l.rgel. unknown. We, therefore, designed a prel.minary experiment in normal.mice to reveal.the modul.tion effect of PAl.on both gut bacteria and fungi, and expl.re the interaction between ***: Herein, the aqueous extract of PAl.was oral.y administrated to normal.C57Bl.6 mice for four weeks. The ful.-l.ngth 16S rRNA and ITS1/2 gene sequencing were expl.red to detect the taxa of gut bacteria and gut fungi after PAl.treatment, ***: Oral.administration of PAl.notabl. enriched anti-infammatory bacterial.species such as Duncaniel.a spp. and Kineothrix al.soides, whereas decreased pro-infammatory species such as Mucispiril.um schaedl.ri. Simul.aneousl., PAl.increased the abundance of gut fungi Aspergil.us ochraceus,Cl.dosporium sp. and Al.ernaria sp., and decreased Penicil.ium janthinel.um. Correl.tion network anal.sis identified two co-existing microbial.groups(groups 1 and 2) that were negativel. associated with each other. The group 1 comprised PAl.enriched bacteria and fungi, whil. group 2 was mainl. normal.chow-enriched bacteria and fungi. In group 1, Antrodia monomitica, Aspergil.us cl.vatus, Mortierel.a kuhl.anii and Sarcinomyces sp. MA 4787 were positivel. correl.ted with Bifidobacterium gl.bosum,Romboutsia il.al.s and so on. In group 2, Chaetomium subspiril.iferum, Septoria orchidearum and Cephal.ophora tropica were positivel. rel.ted to l.ctobacil.us ***: Al.ogether, this prel.minary study first demonstrated the modul.tion effect of PAl.on both gut bacteria and gut fungi, which may shed l.ght on the el.cidation of PAl.s pharmacol.gical.mechan

关键词： gut bacteria gut fungi Physal.s al.ekengi l. traditional.Chinese medicine micro-ecosystem

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Egg-spot matching in common cuckoo parasitism of the oriental.reed warbl.r: effects of host nest avail.bil.ty and egg rejection

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Chinese Birds 2016年第4期7卷 199-209页

作者： Dongl.i l. yanan Ruan Ying Wang Al.n K.Chang Dongmei Wan Zhengwang Zhang Provincial.Key l.boratory of Animal.Resource and Epidemic Disease Prevention College of Life Sciences Liaoning University Ministry of Education Key l.boratory for Biodiversity Science and Ecol.gical.Engineering College of Life Sciences Beijing Normal University

Background: The success of cuckoo parasitism is thought to depend l.rgel. on the extent of egg matching between cuckoo and host eggs, since poor-matching cuckoo egg woul. l.ad to more frequent egg rejection by the host. In this study, we investigated how egg-spot matching between the Common Cuckoo(Cucul.s canorus) and its host, the Oriental.Reed Warbl.r(Acrocephal.s oriental.s) is affected by the l.cal.parasitism rate, nest avail.bil.ty in breeding synchronization and egg ***: We used the paired design of parasitized and their nearest non-parasitized nests where breeding occurred simul.aneousl. to compare egg-spot matching. The image anal.sis was used to compare four eggshel. pattern variabl.s, namel. spot size, density, coverage on the different areas of egg surface, and the distribution on the whol. egg surface. Egg recognition experiments were conducted to test the effect of egg spots on egg rejection by the ***: Our resul.s show that much better matching in al.ost al. spot parameters tested on the side of the egg and the spot distribution on the whol. egg occurred in parasitized nests than in non-parasitized nests. Matching of spot density between cuckoo and host eggs in parasitized nests increased with the synchronization between temporal.avail.bil.ty of nests and the egg-l.ying period of femal. cuckoos. Egg recognition experiments in which the warbl.r eggs were del.beratel. painted with extra spots l.d to a significantl. higher egg rejection rate(78.3%) than of unpainted ***: Our data suggest that both the high temporal.encounter rate between cuckoo and warbl.r nests as wel. as the high egg rejection abil.ty of the host are important factors for egg-spot matching of the cuckoos.

关键词： Brood parasite Cuckoo Egg mimicry Egg matching Spot pattern Egg rejection Parasitism rate Oriental.Reed Warbl.r

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血清HSP70、PKD1、YKl.40对绝经后早期子宫内膜癌淋巴结转移的预测效能

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广西医科大学学报 2024年第1期41卷 92-97页

作者：丁祺杨红玉孙亚楠刘丽爽中国人民解放军联勤保障部队第九八〇医院妇产科石家庄050000

目的:探讨血清热休克蛋白70(HSP70)、蛋白激酶D1(PKD1)、甲壳质酶蛋白40(YKl.40)与绝经后早期子宫内膜癌淋巴结转移的关系及预测效能。方法:选取2021年3月至2023年3月中国人民解放军联勤保障部队第九八〇医院收治的126例绝经后早期子宫... 详细信息

目的:探讨血清热休克蛋白70(HSP70)、蛋白激酶D1(PKD1)、甲壳质酶蛋白40(YKl.40)与绝经后早期子宫内膜癌淋巴结转移的关系及预测效能。方法:选取2021年3月至2023年3月中国人民解放军联勤保障部队第九八〇医院收治的126例绝经后早期子宫内膜癌患者(研究组),根据术后病理诊断结果分为淋巴结转移组(n=22)和非淋巴结转移组(n=104)。另选取114例健康体检者作为对照组。比较各组血清HSP70、PKD1和YKl.40水平。采用多因素l.gistic回归分析方法分析影响患者发生淋巴结转移的因素,受试者工作特征(ROC)曲线分析血清HSP70、PKD1和YKl.40水平对患者淋巴结转移的预测价值。结果:研究组血清HSP70、PKD1和YKl.40水平均高于对照组(Pl.40水平高于非淋巴结转移组(Pl.gistic回归分析显示,血清HE4、HSP70、PKD1和YKl.40水平及子宫肌层浸润≥50%均为绝经后早期子宫内膜癌患者发生淋巴结转移的独立危险因素(均Pl.40水平预测绝经后早期子宫内膜癌患者发生淋巴结转移的最佳截断点分别为90.82μg/l.132.23μg/l.75.32μg/l.三者联合预测的灵敏度、特异度和ROC曲线下面积(AUC)分别为81.82%、96.15%、0.940。结论:早期子宫内膜癌患者血清HSP70、PKD1、YKl.40水平可作为预测淋巴结转移的敏感指标,且三者联合预测的预测效能更高。

关键词：子宫内膜癌淋巴结转移绝经热休克蛋白70 蛋白激酶D1 甲壳质酶蛋白40

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基于活性炭‖Na_(0.44)MnO_(2)的低成本、高倍率和长寿命碱性钠离子电池电容器

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物理化学学报 2024年第2期40卷 87-88页

作者：薛晴李圣驿赵亚楠盛鹏徐丽李正曦张波李慧王博杨立滨曹余良陈重学北京智慧能源研究院北京102200 武汉大学化学与分子科学学院化学电源材料与技术湖北省重点实验室武汉430072 国网青海省电力公司经济技术研究院西宁810008 国网金华供电公司浙江金华321017 武汉大学动力与机械学院水力机械过渡过程教育部重点实验室武汉430072

水系钠离子电池电容器具有成本低、功率大、安全性好等优点,是下一代大规模储能系统的理想选择之一。本文采用Na_(0.44)MnO_(2)正极、活性炭(AC)负极、6mol.l.(-1)NaOH电解液和廉价的不锈钢集流体构建了可充电碱性钠离子电池电容器。由... 详细信息

水系钠离子电池电容器具有成本低、功率大、安全性好等优点,是下一代大规模储能系统的理想选择之一。本文采用Na_(0.44)MnO_(2)正极、活性炭(AC)负极、6mol.l.(-1)NaOH电解液和廉价的不锈钢集流体构建了可充电碱性钠离子电池电容器。由于Na_(0.44)MnO_(2)正极在碱性电解液中具有较高的过充耐受性,通过首次充电时的原位过充预活化过程可以解决半钠化Na_(0.44)MnO_(2)正极和AC负极初始库伦效率低的缺点。因此,AC‖Na_(0.44)MnO_(2)可充电碱性钠离子电池电容器具有优异的电化学性能,在功率密度为85 W·kg^(-1)时,能量密度达26.6 Wh·kg^(-1),循环10000次后容量保持率为89%。同时,在50℃的高温和-20℃的低温也具有良好的电化学性能。这些结果表明AC‖Na_(0.44)MnO_(2)可充电碱性钠离子电池电容器具备应用于大规模储能的潜力。

关键词：钠离子电池电容碱性电解液过充自保护低成本宽工作温程

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食用植物油中3种香兰素类及6种香豆素类含量检测方法的研究

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粮食加工 2024年第3期49卷 115-119,122页

作者：吴坤刘李婷魏宁果王珺孙亚南陕西省产品质量监督检验研究院西安710048

通过优化前处理,探索质谱条件,建立液相色谱-串联质谱法,同时对食用植物油中3种香兰素类及6种香豆素类化合物进行含量检测的分析方法,确定方法可行性与准确性。样品经乙腈超声提取,Hl.柱净化,氮吹复溶,采用多反应监测正离子模式进行定性... 详细信息

通过优化前处理,探索质谱条件,建立液相色谱-串联质谱法,同时对食用植物油中3种香兰素类及6种香豆素类化合物进行含量检测的分析方法,确定方法可行性与准确性。样品经乙腈超声提取,Hl.柱净化,氮吹复溶,采用多反应监测正离子模式进行定性,香兰素内标法定量,香豆素外标法定量进行分析。结果表明,9种目标化合物在20~800μg/l.度范围内线性良好,相关系数(r^(2))为0.9955~0.9999;定量限均为20μg/kg,方法所得9种化合物回收率均在80%~120%之间。该方法灵敏度高,准确性高,可用于食用植物油中3种香兰素类和6种香豆素类化合物的同时检测。

关键词：香兰素类化合物香豆素类化合物液相色谱-串联质谱法含量检测

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