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Dual-functional poly(2-methyl-2-oxazoline)/poly(2-(dimethylamine)ethyl methacrylate)mixed brushes with switchable protein adsorption and antibacterial properties

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Chinese Journal of Chemical Engineering 2024年第2期66卷 19-30页

作者： Kang He Liangyu Zhu yanmei wang CAS Key Laboratory of Soft Matter Chemistry Department of Polymer Science and EngineeringUniversity of Science and Technology of ChinaHefei 230026China

Herein,binary mixed brushes consisting of poly(2-methyl-2-oxazoline)(PMOXA)and poly(2-(dimethylamine)ethyl methacrylate)(PDMAEMA)with different chain lengths were fabricated by successive grafting of NH_(2)-terminated PMOXA and SH-terminated PDMAEMA onto polydopamine-anchored *** mixed-brush coating was characterized by variable-angle spectroscopic ellipsometry,X-ray photoelectron spectroscopy,Fourier transform infrared spectroscopy,zeta potential measurements,water contact angle,and atomic force *** mixed brushes showed tunable surface charge,wettability,and surface roughness,depending on the degree of PDMAEMA swelling under varying pH and ionic strength(Ⅰ).Then the adsorption behaviors of pepsin,bovine serum albumin(BSA),γ-globulin,and lysozyme,four very different proteins with regard to isoelectric point,on the mixed brushes coating were studied by using fluorescence microscopy and surface plasmon *** the chain length of PDMAEMA was about twice as long as PMOXA,the mixed brushes not only had high adsorption capacity for pepsin,BSA,and y-globulin but also had a desorption efficiency of 86.9%,87.1%,and 93.5%,*** is explained that electrostatic attraction between the protonated PDMAEMA and positively charged acidic proteins(pepsin and BSA,whose isoelectric points were below the pK_(a) of PDMAEMA)would drive the intensive adsorption(at pH 3,I=10^(-3)mol·L^(-1)for pepsin,and pH 5,I=10^(-5)mol·L^(-1)for BSA),while desorption was dominated by the hydrophilic PMOXA when PDMAEMA was shrinking(at pH 7,I=10^(-1)mol·L^(-1)for pepsin,and pH 9,I=10^(-1)mol·L^(-1)for BSA).Furthermore,the isoelectric precipitation led to the adsorption of neutral protein(γ-globulin,whose isoelectric point was near the pK_a of PDMAEMA)at pH 7,I=10^(-5)mol·L^(-1),while electrostatic repulsion and antifouling PMOXA triggered the desorption of y-globulin at pH 3,I-10^(-1)mol·L^(-1).However,alkaline protein(lysozyme,whose isoelectric point was higher than

关键词： Switchable conformation transition Controllable protein adsorption Mixed brushes Isoelectric point Antibacterial surface

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Selective chemical degradation of kerogen from Nenjiang Formation of the southern Songliao Basin

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Science China Earth Sciences 2007年第10期50卷 1504-1509页

作者： XIONG YongQiang wang YongQuan wang yanmei State Key Laboratory of Organic Geochemistry Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou 510640China State Key Laboratory of Organic Geochemistry Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou 510640China State Key Laboratory of Organic Geochemistry Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou 510640China

A sequential selective chemical degradation has been performed on the kerogen from the Nenjiang Formation of the southern Songliao Basin by using a series of mild chemical degradations (alkaline hydrolysis, cleavage of ether-bonds and sulfur-bonds, and ruthenium tetroxide (RuO4) oxidation). Subsequently, the GC-MS analyses are carried out on different degradation products. The results show that chemical degradations can release a great number of GC/MS-determinable biomarkers from insoluble kerogen, such as, alkaline hydrolysis products mainly comprise n-alkanes, fatty acids and alkanols; thiophene compounds are predominantly ether-bound to kerogen matrix; the products from the cleavage of sulfur-sulfur and sulfur-carbon bonds in the kerogen include fatty acids, alkanols and some n-alkanes with high carbon numbers; RuO4 oxidation products are predominantly monocarboxylic acids and α,ω-dicarboxylic acids. The distributions of main degradation products indicate that organic matter in this kerogen is predominantly derived from algae and bacteria, and that small amounts of high plant-derived organic matter are possibly combined into kerogen matrix at the late stage by sulfur bonds and other means. This study will provide an important approach for further discussing sources of organic matter in source rocks and their depositional paleoenvironments.

关键词： kerogen, selective chemical degradation, biomarker, Songliao basin

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Genome-wide identification and characterization of phospholipase C gene family in cotton (Gossypium spp.)

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Science China(Life Sciences) 2018年第1期61卷 88-99页

作者： Bing Zhang yanmei wang Jin-Yuan Liu Laboratory of Plant Molecular Biology Center for Plant Biology School of Life Sciences Tsinghua University

Phospholipase C (PLC) are important regulatory enzymes involved in several lipid and Ca2+-dependent signaling *** studies have elucidated the versatile roles of PLC genes in growth, development and stress responses of many plants, however, the systematic analyses of PLC genes in the important fiber-producing plant, cotton, are still deficient. In this study,through genome-wide survey, we identified twelve phosphatidylinositol-specific PLC (PI-PLC) and nine non-specific PLC (NPC) genes in the allotetraploid upland cotton Gossypium hirsutum and nine PI-PLC and six NPC genes in two diploid cotton G. arboretum and ***, respectively. The PI-PLC and NPC genes of G. hirsutum showed close phylogenetic relationship with their homologous genes in the diploid cottons and Arabidopsis. Segmental and tandem duplication contributed greatly to the formation of the gene family. Expression profiling indicated that few of the PLC genes are constitutely expressed, whereas most of the PLC genes are preferentially expressed in specific tissues and abiotic stress conditions. Promoter analyses further implied that the expression of these PLC genes might be regulated by MYB transcription factors and different *** results not only suggest an important role of phospholipase C members in cotton plant development and abiotic stress response but also provide good candidate targets for future molecular breeding of superior cotton cultivars.

关键词： cotton phospholipase C gene duplication fiber development stress response

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High-throughput sequencing-based genome-wide identification of micro RNAs expressed in developing cotton seeds

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Science China(Life Sciences) 2015年第8期58卷 778-786页

作者： wang yanmei DING Yan YU DingWei XUE Wei LIU JinYuan Laboratory of Plant Molecular Biology Center for Plant Biology School of Life Sciences Tsinghua University

Micro RNAs(mi RNAs) have been shown to play critical regulatory roles in gene expression in cotton. Although a large number of mi RNAs have been identified in cotton fibers, the functions of mi RNAs in seed development remain unexplored. In this study, a small RNA library was constructed from cotton seeds sampled at 15 days post-anthesis(DPA) and was subjected to high-throughput sequencing. A total of 95 known mi RNAs were detected to be expressed in cotton seeds. The expression pattern of these identified mi RNAs was profiled and 48 known mi RNAs were differentially expressed between cotton seeds and fibers at 15 DPA. In addition, 23 novel mi RNA candidates were identified in 15-DPA seeds. Putative targets for 21 novel and 87 known mi RNAs were successfully predicted and 900 expressed sequence tag(EST) sequences were proposed to be candidate target genes, which are involved in various metabolic and biological processes, suggesting a complex regulatory network in developing cotton seeds. Furthermore, mi RNA-mediated cleavage of three important transcripts in vivo was validated by RLM-5′ RACE. This study is the first to show the regulatory network of mi RNAs that are involved in developing cotton seeds and provides a foundation for future studies on the specific functions of these mi RNAs in seed development.

关键词： Gossypium hirsutum seed development microRNA (miRNA) target gene GO annotation high-throughput sequencing

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A Restricted Quantum Deniable Authentication Protocol Based on GHZ States

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Chinese Journal of Electronics 2018年第2期27卷 229-233页

作者： SHI Weimin wang yanmei LI Leilei ZHOU Yihua YANG Yuguang JIANG Nan College of Computer Science and Technology Beijing University of Technology College of Computer Science and Technology Beijing University of Posts and Telecommunications

Utilizing the property of unitary transformation and Greenberger-Horne-Zeilinger(GHZ) states,a restricted quantum deniable authentication protocol is proposed. The protocol can provide that the sender can deny the content of the sent message, but cannot deny taking part in a certain communication. Security analysis results show that this protocol can satisfy known key security and the basic security requirements of restricted deniable authentication protocol such as completeness, restricted deniability, no-counterfeiting and can withstand impersonation attack, intercept-resend attack.

关键词： Quantum deniable authentication Restricted Greenberger-Horne-Zeilinger(GHZ) states

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Overexpression of BnKCS1-1,BnKCS1-2,and BnCER1-2 promotes cuticular wax production and increases drought tolerance in Brassica napus

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The Crop Journal 2020年第1期8卷 26-37页

作者： yanmei wang Shurong Jin Yi Xu Shuai Li Shuangjuan Zhang Zheng Yuan Jiana Li Yu Ni College of Agronomy and Biotechnology Academy of Agricultural SciencesSouthwest University

Higher amounts of cuticular wax in plants have been associated with improved plant stress tolerance and increased potential for industrial *** this study,orthologs of KCS1 and CER1 in Arabidopsis,designated BnKCS1-1,BnKCS1-2,and BnCER1-2,were isolated from Brassica *** of BnKCS1-1 and BnKCS1-2 in *** were induced by abscisic acid(ABA)and drought treatment,while transcription of BnCER1-2 was induced only by drought *** three gene transcripts decreased significantly when plants were treated with methyl jasmonate(MeJA)or subjected to cold *** of BnKCS1-1,BnKCS1-2,and BnCER1-2 under the control of the CaMV35S promoter led to a significant increase in cuticular wax on transgenic *** ***1-1 and BnKCS1-2 overexpression led to similar differences from non-transformed plants,with significantly higher levels of aldehydes(C29 and C30),alkanes(C28,C29,and C31)and secondary alcohols(C28 and C29),and a significantly lower level of C29 *** of BnCER1-2 led to an increase in alkanes(C27,C28,C29,and C31),a decrease in secondary alcohols(C28 and C29),and insignificant changes in other wax *** electron microscopy revealed that overexpression of BnKCS1-1,BnKCS1-2,and BnCER1-2 in *** resulted in a higher density of wax crystals on the leaf surface than observed in non-transformed *** plants showed a reduced rate of water loss and increased drought tolerance compared to non-transformed *** results suggest that BnKCS1-1,BnKCS1-2,and BnCER1-2 gene products can modify the cuticular wax of *** cuticular waxes using transgenic approaches is a new strategy for genetic improvement of plant drought tolerance and provides an opportunity for development of *** as a surface-wax crop.

关键词： Brassica napus Cuticular wax Drought tolerance Overexpression Transformation

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Spatial confinement tuning of quenched disorder effects and enhanced magnetoresistance in manganite nanowires

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Science China(Physics,Mechanics & Astronomy) 2020年第3期63卷 106-111页

作者： Yang Yu Qiang Li Qian Shi YinYan Zhu HanXuan Lin Hao Liu HongYan Chen Tian Miao Yu Bai yanmei wang WenTing Yang WenBin wang HangWen Guo LiFeng Yin Jian Shen State Key Laboratory of Surface Physics and Department of Physics Fudan UniversityShanghai 200433China Institute for Nanoelectronic Devices and Quantum Computing Fudan UniversityShanghai 200433China Collaborative Innovation Center of Advanced Microstructures Nanjing 210093China

Complex oxides have rich functionalities and advantages for future *** many systems,quenched disorder often holds the key to determine their physical properties,and these properties can be further tuned by chemical ***,understanding the role of quenched disorder is complicated because chemical doping simultaneously alters other physical variables such as local lattice distortions and electronic and magnetic ***,we show that spatial confinement is an effective approach to tuning the level of quenched disorder in a complex-oxide system while leaving other physical variables largely *** the confinement of a manganite system down to quasi-one-dimensional nanowires,we observed that the nature of its metal-insulator phase transition exhibits a crossover from a discontinuous to a continuous characteristic,in close accordance with quenched disorder *** argue that the quenched disorder,finite size,and surface effects all contribute to our experimental ***,with reduced nanowire width,the magnetoresistance shows substantial enhancement at low *** findings offer new insight into experimentally tuning the quenched disorder effect to achieve novel functionalities at reduced dimensions.

关键词： quenched disorder manganite nanowires phase transition colossal magnetoresistance

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Mechanism of aseptic inflammation upon the inner ear injury

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Journal of Bio-X Research 2020年第2期3卷 72-77页

作者： wang yanmei Ren Dongdong ENT Institute and Otorhinolaryngology Department Affiliated Eye and ENT Hospital of Fudan UniversityShanghaiChina Shanghai Auditory Medical Center ShanghaiChina Key Laboratory of Hearing Science Ministry of HealthShanghaiChina

Many factors can cause inner ear injury,such as noise exposure,chemicals,viral infection,and *** main pathological manifestations of inner ear injury are local hypoxia-ischemia,micro-trauma,and an increased level of reactive oxygen species and inflammatory *** contribution of the inflammatory response to the mediation of cochlear and vestibular pathologies has received increasing attention in recent *** inflammation can devastate audition and balance,which can lead to many typical clinical inner ear *** this review,we will discuss the most pertinent and recent research on inflammatory mechanisms in inner ear *** will also discuss the pathophysiology of some common and significant ear diseases,such as sudden sensorineural hearing loss,age-related hearing loss,noise-induced hearing loss,and Meniere’s disease.

关键词： age-related hearing loss immune response inflammatory response macrophages sudden sensorineural hearing loss

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Application Process of Iodine System in the Gas Hydrate

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Acta Geologica Sinica(English Edition) 2015年第A1期89卷 386-387页

作者： FU Piaoer CAO Jun wang yanmei ZHANG Xin CHEN Sihai Guangzhou Marine Geological Survey Guangzhou 510075 Key Laboratory of Marine Mineral Resources Ministry of Land and Resources Guangzhou 510075

As a new energy source, gas hydrate has shown important economic significance. Although the total mass remains unclear, it is consensus that the gas hydrate is a major hydrocarbon reservoir in Earth＇s surface （Milkov, 2004）. Bottom-simulating reflector （BSR） on seismic profiles is a main means to identify the gas-hydrate deposits in current surveys. However, scientific drilling indicated BSR did not be found in some of sea area, where collected gas hydrate. BSR is neither the best nor only indicator for the presence of gas hydrates. Thus, more or comprehensive means are need to identify the gas hydrate accurately in the ocean.

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Current HSE Management in the Oil Industry and Suggestions for Its Improvement

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Petroleum Science 2005年第3期2卷 73-79页

作者： Feng Lianyong wang yanmei School of Business Administration China University of Petroleum ChinaBeijing 102249

Because of some specific characteristics of petroleum and petrochemical industry, in normal operation there are always risks of injury and pollution, especially when accidents happen. This is a severe problem and the situation is serious. The first reason is the lack of attention. Leaders of corporations attach too much importance to profit and neglect HSE （Health, Safety and Environment） management. Second, the governmental supervisory system is weak. Third, objective conditions for the petroleum and petrochemical industry bring difficulties to the construction of an effective HSE system. This article proposes that corporations, governments at all levels and the society should strive together to alter the situation and promote the implementation of a much better HSE management system in industry. First of all, top managers of petroleum corporations are expected to think more of their social responsibilities. Second, corporations should change their ideas about management so that they may possibly realize that it is helpful for getting better rewarded to establish and strengthen the HSE system and to take social responsibilities. Third, corporations should build and develop the HSE management system constantly to settle the staff＇s health and safety problems and improve the working environment effectively. Meanwhile, encouragement should be offered and some tough measures taken by the government to strengthen supervision. A new standard for appraising corporations should be set across the whole society to encourage a healthy development of the corporations.

关键词： World oil HSE social responsibility strategy environment

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