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Potential dissemination mechanism of the tetC gene in Aeromonas media from the aerobic biofilm reactor under oxytetracycline stresses

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Journal of Environmental Sciences 2021年第7期33卷 90-99页

作者： yanhong shi Yu Zhang Xiangyang Wu Hong Zhang Min Yang Zhe Tian School of the Environment and Safety Engineering Jiangsu UniversityZhenjiang 212013China State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China

The tet C gene has been found to be one of the most widely distributed tetracycline resistance( tet) genes in various environmental niches, but the detailed dissemination mechanisms are still largely unknown. In the present study, 11 tet C-containing Aeromonas media strains were isolated from an aerobic biofilm reactor under oxytetracycline stresses, and the genome of one strain was sequenced using the Pac Bio RSII sequencing approach to reveal the genetic environment of tet C. The tet C gene was carried by an IS 26 composite transposon, named Tn 6434. The tet C-carrying Tn 6434 structure was detected in all of the A. media strains either in a novel plasmid p Aeme2( n = 9) or other DNA molecules( n = 2) by PCR screening. The NCBI database searching result shows that this structure was also present in the plasmids or chromosomes of other 13 genera, indicating the transferability of Tn 6434. Inverse PCR and sequencing confirmed that Tn 6434 can form a circular intermediate and is able to incorporate into a preexisting IS 26 element, suggesting that Tn 6434 might be responsible for the dissemination of tet C between different DNA molecules. This study will be helpful in uncovering the spread mechanism of tet genes in water environments.

关键词： Aeromonas media tetC IS 26 Circular intermediate Inverse PCR

Genetic characterization and potential molecular dissemination mechanism of tet(31) gene in Aeromonas caviae from an oxytetracycline wastewater treatment system

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Journal of Environmental Sciences 2019年第2期31卷 259-266页

作者： yanhong shi Zhe Tian Sébastien Olivier Leclercq Hong Zhang Min Yang Yu Zhang State Key Laboratory of Environmental Aquatic Chemistry Research Centre for Eco-Environmental SciencesChinese Academy of Sciences University of Chinese Academy of Sciences INRA UMR1282 Infectiology and Public Health Fran?ois Rabelais University UMR1282 Infectiology and Public Health

Recently, the rarely reported tet(31) tetracycline resistance determinant was commonly found in Aeromonas salmonicida, Gallibacterium anatis, and Oblitimonas alkaliphila isolated from farming animals and related environment. However, its distribution in other bacteria and potential molecular dissemination mechanism in environment are still unknown. The purpose of this study was to investigate the potential mechanism underlying dissemination of tet(31) by analysing the tet(31)-carrying fragments in A. caviae strains isolated from an aerobic biofilm reactor treating oxytetracycline bearing wastewater. Twenty-three A. caviae strains were screened for the tet(31) gene by polymerase chain reaction(PCR). Three strains(two harbouring tet(31), one not) were subjected to whole genome sequencing using the PacBio RSII platform. Seventeen A. caviae strains carried the tet(31) gene and exhibited high resistance levels to oxytetracycline with minimum inhibitory concentrations(MICs)ranging from 256 to 512 mg/L. tet(31) was comprised of the transposon Tn6432 on the chromosome of A. caviae, and Tn6432 was also found in 15 additional tet(31)-positive A. caviae isolates by PCR. More important, Tn6432 was located on an integrative conjugative element(ICE)-like element, which could mediate the dissemination of the tet(31)-carrying transposon Tn6432 between bacteria. Comparative analysis demonstrated that Tn6432 homologs with the structure ISCR2-ΔphzF-tetR(31)-tet(31)-ΔglmM-sul2 were also carried by A. salmonicida, G. anatis, and O. alkaliphila, suggesting that this transposon can be transferred between species and even genera. This work provides the first report on the identification of the tet(31) gene in A. caviae, and will be helpful in exploring the dissemination mechanisms of tet(31) in water environment.

关键词： Aeromonas caviae tet(31) gene ISCR2 ICE-like element Wastewater

Sensitive and rapid detection of two toxic microalgae Alexandrium by loop-mediated isothermal amplification

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Acta Oceanologica Sinica 2012年第2期31卷 139-146页

作者： ZHANG Fengying shi yanhong JIANG Keji XU Zhaoli MA Lingbo East China Sea Fisheries Research Institute Chinese Academy of Fishery SciencesShanghai 200090China Key and Open Laborutory of Marine and Estuarine Fisheries Ministry of Agriculture of ChinaShanghai 200090China

A loop-mediated isothermal amplification （LAMP） assay was designed and evaluated for rapid de- tection of the toxic microalgae Alexandrium catenella and A. minutum, which can produce paralytic shellfish poisoning （PSP）. Two sets of four specific primers targeting these two species were derived from the sequence of internal transcribed spacer （ITS） of ribosomal DNA. The method worked well in less than an hour under isothermal conditions of 65℃. LAMP specificity was validated in closely related algae as a comparison, suggesting the strict specificity of the LAMP primers. Two visual inspection approaches were feasible to interpret the positive or negative results. The detection lim- its of A. catenella and A. minutum samples using the LAMP assay were found to be 5.6 and 4.5 pg DNA, respectively. The sensitivity of this LAMP assay was 10 or 100-fold higher than Polymerase Chain Reaction （PCR） method in detecting the two microalgae. These characteristics of species specificity, sensitivity, and rapidity suggest that this method has the potentiality in the monitoring of red tide caused by A. catenella and A. minutum.

关键词： Alexandrium eatenella Alexandrium minutum detection loop-mediated isothermal amplification (LAMP) ribosomal DNA internal transcribed spacer (ITS)

Severe Henoch-Schonlein purpura with infliximab forulcerative colitis

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World Journal of Gastroenterology 2015年第19期21卷 6082-6087页

作者： Yang Song yanhong shi Chong He ChangQin Liu JunShan Wang YuJie Zhao YanMin Guo RuiJin Wu XiaoYue Feng ZhanJu Liu Yang Song Yan-Hong Shi Chong He Chang-Qin Liu Jun-Shan Wang Yu-Jie Zhao Yan-Min Guo Rui-Jin Wu Xiao-Yue Feng Zhan-Ju Liu Department of Gastroenterology Shanghai Tenth People's Hospital Tongji University Shanghai 200072 China.

Infliximab （IFX） is an anti-tumor necrosis factorchimeric antibody that is effective for treatment ofautoimmune disorders such as Crohn＇s disease andulcerative colitis （UC）. IFX is well tolerated with alow incidence of adverse effects such as infections,skin reactions, autoimmunity, and *** manifestations can appear as infusionreaction, vasculitis, cutaneous infections, psoriasis,eczema, and skin cancer. Here, we present anunusual case of extensive and sporadic subcutaneousecchymosis in a 69-year-old woman with severe UC,partial colectomy and cecostomy, following her initialdose of IFX. The reaction occurred during infliximabinfusion, and withdrawal of IFX led to gradual alleviationof her symptoms. We concluded that Henoch-Sch？nleinpurpura, a kind of leukocytoclastic vasculitis, mighthave contributed to the development of the *** the precise mechanisms of the vasculitis arestill controversial, such a case highlights the importanceof subcutaneous adverse effects in the management ofUC with IFX.

关键词： Henoch-Sch?nlein purpura Infliximab Vasculitis Subcutaneous ecchymosis Ulcerative colitis

Construction of MnO2 Nanowire for a High-Performance Lithium Ion Supercapacitor

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Research and Application of Materials Science 2019年第1期1卷 18-23页

作者： Wenbo Wang yanhong shi Yang Su Yihai Wang Haizhu Sun National&Local United Engineering Laboratory for Power Battery College of ChemistryNortheast Normal UniversityChangchunChina

Developing lithium ion capacitors possessing both brilliant energy and power density is still significant for numerous *** this paper,we synthesized MnO2 nanowires via a simple hydrothermal *** nanostructure MnO2 can expose more electrochemical sites and thus optimize the kinetics of Li+.Moreover,we used MnO2 nanowires(MnO2 NWs)as anode and a N-doped porous carbon(NPC)as cathode to assemble lithium ion capacitors(MnO2 NWs//NPC LIC).Compared to the traditional supercapacitor with aqueous electrolyte,the MnO2 NWs//NPC LIC exhibits a wider voltage of 0-4.2 V,which is helpful to enhance its energy and power ***,MnO2 NWs//NPC LIC can deliver an excellent capacity of 150 mAh g-1 with an excellent energy density of 82.7 Wh kg-1 and power density of 1.05 kW ***,a good cyclic stability of LICs with a 20%retention after 1000 times charge and discharge process proves its practical potential,indicating a good promising for the application in storage devices.

关键词： Manganese dioxide Nanostructure N-doped porous carbon Lithium-ion supercapacitor

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频率分集阵列稳态波束形成方法

西安电子科技大学学报 2016年第5期43卷 41-45,166页

作者：徐艳红史小卫文珺西安电子科技大学天线与微波技术重点实验室陕西西安710071 广西大学计算机与电子信息学院广西南宁530004

针对频率分集阵列天线的发射方向图随时间变化的问题,提出了一种脉冲体制频率分集阵列天线稳态波束形成方法,并对其发射方向图特性进行分析.频率分集阵列天线在天线单元间采用微小的频率步进可形成距离角度二维依赖的发射方向图.相比传... 详细信息

针对频率分集阵列天线的发射方向图随时间变化的问题,提出了一种脉冲体制频率分集阵列天线稳态波束形成方法,并对其发射方向图特性进行分析.频率分集阵列天线在天线单元间采用微小的频率步进可形成距离角度二维依赖的发射方向图.相比传统相控阵天线,频率分集阵列天线无需移相器,因此其具有成本低、复杂度小以及适合阵列天线小型化设计等优点.同时,其天线发射方向图的距离角度二维依赖特性,大大提高了阵列天线波束扫描的灵活性.仿真实验验证了本文方法的有效性.

关键词：频率分集阵列脉冲体制发射方向图波束扫描距离角度依赖

合理使用眼内液检测,辅助眼底疾病的精准诊疗

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中华眼底病杂志 2021年第7期37卷 497-502页

作者：陶勇石燕红首都医科大学附属朝阳医院眼科北京100020

眼底疾病种类繁多、病因复杂,既可以由代谢因素引起,也可以是炎症因素所致。荧光素眼底血管造影、光相干断层扫描等眼底影像学检查是诊断眼底疾病的主要方式,当其在判断病因和早期诊断时若能合理结合眼内液检测技术,优势会更大。眼内液... 详细信息

眼底疾病种类繁多、病因复杂,既可以由代谢因素引起,也可以是炎症因素所致。荧光素眼底血管造影、光相干断层扫描等眼底影像学检查是诊断眼底疾病的主要方式,当其在判断病因和早期诊断时若能合理结合眼内液检测技术,优势会更大。眼内液是眼球内液体的总称,包括房水、玻璃体液等,其可以检验的分子成分包括DNA、RNA、抗原、抗体、细胞因子。随着分子检测技术和设备的进步,眼内液检测作为循证手段逐步被更多的眼底病专家共识和指南纳入,主要应用于感染性眼底疾病和伪装综合症的诊疗。合理地使用眼内液检测,有助于提升眼底疾病的个性化诊治效果,并减少不必要的药物过度使用。但值得注意的是,眼内液检测只是诸多工具中的一种,不能取代其他检查和临床经验。过度的眼内液检测,不仅因为有创增加了临床感染的风险,同时也会加重患者的负担。

关键词：眼房水视网膜疾病细胞因子类述评眼内液检测

Influence of the Calmodulin Antagonist EBB on Cyclin B1 and Cdc2-p34 in Human Drug-resistant Breast Cancer MCF-7/ADR Cells

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Chinese Journal of Clinical Oncology 2008年第2期5卷 108-112页

作者： Xu shi yanhong Cheng Linglin Zou Dongsheng Xiong Yuan Zhou Ming Yang Dongmei Fan Xiaohua Dai Chunzheng Yang Huifang Zhu National Key Laboratory of Experimental Hematology Institute of Hematology ＆ Hospital of Blood Diseases Chinese Academy of Medical Sciences ＆ Peking Union Medical College Tianjin 300020 China.

OBJECTIVE To investigate the influence of O-(4-ethoxyl-butyl)- berbamine(EBB)on the expression of cyclin B1 and cdc2-p34 in the human drug-resistant breast cancer MCF-7/ADR cell line. METHODS The MTT assay was used to assess the cytotoxicity of *** levels of EBB were added to different cell lines at series of time points solely or combined with doxorubicin(DOX) to detect the effect on the expression of cyclinB1 and cdc2-p34 by Western ***2-p34 tyrosine phosphorylation was detected by *** addition,apoptosis and cytoplastic Ca 2+ concentrations were systematically examined by laser scanning confocal microscopy(LSCM). RESULTS EBB showed little inhibitory activity on human umbilical vein endothelial cells(ECV304),whereas EBB inhibited cell growth(IC50 range,4.55~15.74μmol/L)in a variety of sensitive and drug-resistance cell *** also down-regulated the expression of cyclin B1 and cdc2-p34 in a concentration and time dependent manner,which was an important reason for the G2/M phase *** was shown to induce apoptosis of MCF-7/ADR cells while increasing the level of cytoplastic Ca 2+ . CONCLUSION The low cytotoxicity of EBB suggests it may be useful as a rational reversal *** effect of EBB on cell cycle arrest and related proteins,apoptosis,and cytoplastic Ca 2+ concentration may be involved in reversing multidrug resistance.

关键词： EBB cell cycle cyclinB1 cdc2-p34 apoptosis Ca^2+.

炎症性肠病中免疫调节剂和生物制剂的降级治疗

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胃肠病学 2021年第1期26卷 1-8页

作者：施嫣红刘占举同济大学附属第十人民医院消化内科 200072

免疫调节剂和生物制剂在炎症性肠病(IBD)的治疗中广泛应用,可有效诱导疾病缓解,但这些药物与严重不良事件相关,且长期使用导致治疗费用不断增加。因此,IBD患者一旦病情缓解,即需考虑药物降级治疗的安全性和可行性,此时须权衡持续用药的... 详细信息

免疫调节剂和生物制剂在炎症性肠病(IBD)的治疗中广泛应用,可有效诱导疾病缓解,但这些药物与严重不良事件相关,且长期使用导致治疗费用不断增加。因此,IBD患者一旦病情缓解,即需考虑药物降级治疗的安全性和可行性,此时须权衡持续用药的风险与疾病复发的风险。本文对已发表的关于克罗恩病和溃疡性结肠炎免疫调节剂和生物制剂降级治疗的文献进行评估,明确了停药后IBD复发的危险因素,如疾病活动性、疾病预后特征和既往病程因素,并讨论降级治疗策略和积极的药物监测,以期为临床实践提供一定指导。

关键词： Crohn病结肠炎,溃疡性免疫调节剂生物制剂降级治疗