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Controlled synthesis of nanosized Si by magnesiothermic reduction from diatomite as anode material for Li-ion batteries

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International Journal of Minerals,Metallurgy and Materials 2020年第4期27卷 515-525页

作者： Li-fen Guo Shi-yun Zhang Jian xie Dong Zheng Yuan Jin Kang-yan Wang Da-gao Zhuang Wen-quan Zheng Xin-bing Zhao State Key Laboratory of Silicon Materials School of Materials Science and EngineeringZhejiang UniversityHangzhou 310027China Key Laboratory of Advanced Materials and Applications for Batteries of Zhejiang Province Hangzhou 310027China Hangzhou Branch Zhejiang Zotye Automobile Corporation LimitedHangzhou 310018China Shanghai Han Xing Science and Technology Co. Ltd.Shanghai 201322China

Li-ion batteries(LIBs)have demonstrated great promise in electric vehicles and hybrid electric ***,commercial graphite materials,the current predominant anodes in LIBs,have a low theoretical capacity of only 372 mAh·g?1,which cannot meet the everincreasing demand of LIBs for high energy *** Si is considered an ideal form of Si for the fabrication of LIB anodes as Si–C *** of nanoscale Si in a facile,cost-effective way,however,still poses a great *** this work,nanoscale Si was prepared by a controlled magnesiothermic reaction using diatomite as the Si *** was found that the nanoscale Si prepared under optimized conditions(800°C,10 h)can deliver a high initial specific capacity(3053 mAh·g?1 on discharge,2519 mAh·g?1 on charge)with a high first coulombic efficiency(82.5%).When using sand-milled diatomite as a precursor,the obtained nanoscale Si exhibited a well-dispersed morphology and had a higher first coulombic efficiency(85.6%).The Si–C(Si:graphite=1:7 in weight)composite using Si from the sand-milled diatomite demonstrated a high specific capacity(over 700 mAh·g?1 at 100 mA·g?1),good rate capability(587 mAh·g?1 at 500 mA·g?1),and a long cycle life(480 mAh·g?1 after 200 cycles at 500 mA·g?1).This work gives a facile method to synthesize nanoscale Si with both high capacity and high first coulombic efficiency.

关键词： silicon anode magnesiothermic reduction diatomite Li-ion batteries

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Combined central retinal artery and vein occlusion associated with primary antiphospholipid syndrome treated by injection of dexamethasone intravitreal implant and anticoagulant therapy: a case report

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International Journal of Ophthalmology(English edition) 2022年第6期15卷 1027-1029页

作者： Bing-Wen Lu Li-Ke xie Xiao-Feng Hao Xiao-yan Zhang Jie Luo Department of Ophthalmology Ophthalmology Hospital of China Academy of Traditional Chinese MedicineBeijing 100040China

Dear Editor,We present a case of combined central retinal artery and vein occlusion associated with primary antiphospholipid syndrome(APS) treated by injection of dexamethasone intravitreal implant(Ozurdex;) and anticoagulant therapy. Written informed consent was obtained by the patient to allow publication of this case. The study was conducted in accordance with the tenets of the Declaration of Helsinki.

关键词： injection occlusion implant

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细胞周期蛋白依赖性激酶4/6抑制剂对多发性骨髓瘤细胞B细胞成熟抗原和程序性死亡受体配体1表达的影响

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中华实用诊断与治疗杂志 2024年第6期38卷 546-551页

作者：谢适意于珍魏晓晶邹鹤松张悦邱录贵徐燕中国医学科学院北京协和医学院血液病医院(中国医学科学院血液学研究所)血液与健康全国重点实验室国家血液系统疾病临床医学研究中心细胞生态海河实验室天津医学健康研究院天津300020 天津医科大学第二医院血液内科天津300211 郑州人民医院河南中医药大学人民医院血液内科河南郑州450053

目的探讨细胞周期蛋白依赖性激酶4/6(CDK4/6)抑制剂对多发性骨髓瘤(MM)细胞免疫治疗相关分子B细胞成熟抗原(BCMA)、程序性死亡受体配体1(PD-L1)表达及MM细胞和正常微环境细胞凋亡的影响。方法对数生长期H929细胞分为阿贝西利组、帕博... 详细信息

目的探讨细胞周期蛋白依赖性激酶4/6(CDK4/6)抑制剂对多发性骨髓瘤(MM)细胞免疫治疗相关分子B细胞成熟抗原(BCMA)、程序性死亡受体配体1(PD-L1)表达及MM细胞和正常微环境细胞凋亡的影响。方法对数生长期H929细胞分为阿贝西利组、帕博西尼组、DMSO组,于细胞培养基中分别加入2μmol/L阿贝西利、5μmol/L帕博西尼和等体积DMSO处理,处理1、3、5 d时,采用流式细胞术检测3组细胞BCMA、PD-L1相对平均荧光强度。2020年1月—2023年6月中国医学科学院血液病医院诊治MM患者3例,采集患者骨髓,采用淋巴细胞分离液分离骨髓单个核细胞,分为阿贝西利组、帕博西尼组、DMSO组,分别给予1μmol/L阿贝西利、10μmol/L帕博西尼和等体积DMSO处理,采用流式细胞术检测CD138+肿瘤细胞和CD138-正常微环境细胞相对细胞存活率。结果帕博西尼组处理1、3、5 d时BCMA相对平均荧光强度(1.37±0.04、2.36±0.11、3.99±0.17)均高于阿贝西利组(0.69±0.02、1.03±0.05、1.69±0.06)(P0.05);阿贝西利组处理5 d时BCMA相对平均荧光强度高于DMSO组(P0.05);阿贝西利组、帕博西尼组处理1、3、5 d时BCMA相对平均荧光强度均依次增高(P0.05);阿贝西利组、帕博西尼组处理3 d时均高于处理1、5 d时(P0.05);阿贝西利组处理24、48 h时骨髓单个核细胞CD138-相对细胞存活率与DMSO组比较差异均无统计学意义(P>0.05)。结论 CDK4/6抑制剂上调MM细胞BCMA、PD-L1表达,对MM细胞有选择性杀伤作用,对正常微环境细胞无明显影响。

关键词：多发性骨髓瘤细胞周期蛋白依赖性激酶4/6 阿贝西利帕博西尼 B细胞成熟抗原程序性死亡受体配体1

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Plastic deformation wear in modified medium manganese steel

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China Foundry 2007年第3期4卷 194-197页

作者： YUAN Hai-lun xie Jing-pei WANG Ai-qin WANG Wen-yan WANG Cheng . Huazhong University of Science and Technology School of Material Science and Engineering Henan University of Science and Technology Luoyang 471003 China

A medium manganese steel with high wear-resistance,strength and toughness has been produced with addition of a complex modifier (or refining agent) containing Nb,N,RE and Si-Ca. The results showed that the wear resistance,strength and toughness of the modified medium manganese steel are respectively 1.92 times,1.45 times and 3.63 times as high as that of the referenced unmodified medium manganese steel. The plastic deformation characteristic involved in the wear mechanism of the modified medium manganese steel was investigated by means of plastic-elasticity calculation and TEM electro-microscopy. The relationship between wear resistance and yield strength of the steel was established. Since the wear volume W is proportional to the square of the loading and to the numbers of the abrasives,and inversely proportional to the square of the yield strength of the materials,the wear resistance can be substantially improved by the enhancement of yield strength of the materials. The calculation results generally agreed with the experimental results.

关键词： modified medium manganese steel plastic deformation wear yield strength

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Serum Bilirubin and 6-min Walk Distance as Prognostic Predictors for Inoperable Chronic Thromboembolic Pulmonary Hypertension： A Prospective Cohort Study

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Chinese Medical Journal 2015年第23期128卷 3125-3131页

作者： Juan-Ni Gong Zhen-Guo Zhai Yuan-Hua yang yan Liu Song Gu Tu-Guang Kuang Wan-Mu xie Ran Miao Chen Wang Department of Pulmonary and Critical Care Medicine Beijing Institute of Respiratory Medicine Beijing Chao-Yang Hospital Capital Medical University Beijing 100020 China Department of Respiratory Medicine and Critical Care Medicine China-Japan Friendship Hospital Beijing 100029 China Department of Respiratory Medicine Capital Medical University Beijing 100069 China Department of Cardiovascular Surgery Beijing Chao-Yang Hospital Capital Medical University Beijing 100020 China Department of Clinical Laboratory Beijing Instituteof Respiratory Medicine Beijing Chao-Yang Hospital Capital Medical University Key Laboratory of Respiratory and Pulmonary Circulation Disorders Beijing 100020 China China-Japan Friendship Hospital National Clinical Research Center of Respiratory Medicine Beijing 100029 China

Background： Inoperable chronic thromboembolic pulmonary hypertension （CTEPH） is a severe clinical syndrome characterized by right cardiac failure and possibly subsequent liver dysfunction. However, whether serum markers of liver dysfunction can predict prognosis in inoperable CTEPH patients has not been determined. Our study aimed to evaluate the potential role of liver function markers （such as serum levels of transaminase, bilirubin, and gamma-glutamyl transpeptidase [GGT]） combined with 6-min walk test in the prediction of prognosis in patients with inoperable CTEPH. Methods： From June 2005 to May 2013, 77 consecutive patients with inoperable CTEPH without confounding co-morbidities were recruited for this prospective cohort study. Baseline clinical characteristics and 6-min walk distance （6MWD） results were collected. Serum biomarkers of liver function, including levels of aspartate aminotransferase, alanine aminotransferase, GGT, uric acid, and serum bilirubin, were also determined at enrollment. All-cause mortality was recorded during the follow-up period. Results： During the follow-up, 22 patients （29%） died. Cox regression analyses demonstrated that increased serum concentration of total bilirubin （hazard ratio [HR] = 7.755, P 〈 0.001）, elevated N-terminal of the prohormone brain natriuretic peptide （HR = 1.001, P = 0.001）, decreased 6MWD （HR = 0.990, P 〈 0.001）, increased central venous pressure （HR = 1.074, P = 0.040）, and higher pulmonary vascular resistance （HR = 1.001, P = 0.018） were associated with an increased risk of mortality. Serum concentrations of total bilirubin （HR = 4.755, P = 0.007） and 6MWD （HR = 0.994, P = 0.017） were independent prognostic predictors for CTEPH patients. Patients with hyperbilirubinemia （≥23.7 μmol/L） had markedly worse survival than those with normobilirubinemia. Conclusion： Elevated serum bilirubin and decreased 6MWD are potential predictors for poor prognosis in inoperable CTEPH.

关键词： Chronic Thromboembolic Pulmonary Hypertension Heart Failure Liver Function Prognosis

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银星养脑方超高效液相色谱指纹图谱的建立及4种成分含量测定

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中国药业 2024年第16期33卷 62-67页

作者：蒋翠平陶叶琴成紫嫣严建业熊丹谢宗明湖南省浏阳市中医医院湖南浏阳410300 湖南中医药大学附属第二中西医结合医院湖南浏阳410300 湖南中医药大学药学院湖南长沙410208

目的建立银星养脑方超高效液相色谱(UPLC)指纹图谱,并测定指认出的4种成分的含量。方法色谱柱为ACQUITY UPLC?BEH C_(18)柱(100 mm×2.1 mm,1.7μm),流动相为0.1%磷酸水溶液-乙腈(梯度洗脱),流速为0.4 mL/min,检测波长为321 nm,柱温为30... 详细信息

目的建立银星养脑方超高效液相色谱(UPLC)指纹图谱,并测定指认出的4种成分的含量。方法色谱柱为ACQUITY UPLC?BEH C_(18)柱(100 mm×2.1 mm,1.7μm),流动相为0.1%磷酸水溶液-乙腈(梯度洗脱),流速为0.4 mL/min,检测波长为321 nm,柱温为30℃,进样量为1μL。采用中药色谱指纹图谱相似度评价系统(2012 A版)建立12批样品的UPLC指纹图谱,并进行相似度评价,确定共有峰并指认;采用聚类分析和主成分分析对样品进行分类和质量评价;采用UPLC法测定样品指认出的成分含量。结果12批样品UPLC指纹图谱中有30个共有峰,可确定归属银杏叶、蜜远志、川芎,指认出其中4种成分,分别为阿魏酸、3,6'-二芥子酰基蔗糖、异银杏素、金松双黄酮。样品相似度均大于0.990。样品共聚为4类;7个主成分累积方差贡献率达94.100%。4种成分质量浓度分别在3.36~67.20μg/mL,5.10~102.00μg/mL,5.00~100.00μg/mL,2.50~50.00μg/mL范围内与峰面积线性关系良好(R^(2)≥0.9980,n=5);精密度、稳定性、重复性试验结果的RSD均小于3.0%;平均加样回收率为99.01%~101.19%,RSD为0.22%~1.01%(n=6)。结论该方法简单可行、专属性强、重复性好,可用于银星养脑方的质量评价;也为银星养脑片的质量控制和二次开发奠定了前期基础。

关键词：银星养脑方超高效液相色谱法指纹图谱含量测定质量评价

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橘皮竹茹汤对化疗性异食癖大鼠模型5-羟色胺3受体蛋白和mRNA表达的影响

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上海中医药杂志 2024年第4期58卷 19-25页

作者：米金霞艾纯颖李敏吴中华张超超谢燕上海中医药大学公共健康学院上海201203 上海中医药大学专家委员会办公室上海201203 上海中医药大学科技实验中心上海201203

目的观察橘皮竹茹汤对化疗性异食癖大鼠模型止呕作用及对5-羟色胺3受体(5-HT3R)蛋白和mRNA的影响。方法将36只Wistar雄性大鼠随机分为正常对照组、中药对照组(橘皮竹茹汤10.6 g/kg,不造模)、模型组、昂丹司琼组(2.6 mg/kg)、橘皮竹茹汤... 详细信息

目的观察橘皮竹茹汤对化疗性异食癖大鼠模型止呕作用及对5-羟色胺3受体(5-HT3R)蛋白和mRNA的影响。方法将36只Wistar雄性大鼠随机分为正常对照组、中药对照组(橘皮竹茹汤10.6 g/kg,不造模)、模型组、昂丹司琼组(2.6 mg/kg)、橘皮竹茹汤低剂量组(5.3 g/kg)和橘皮竹茹汤高剂量组(10.6 g/kg),每组6只。各组大鼠给予相应干预措施6 d(每日2次)后,采用腹腔注射顺铂(6 mg/kg)的方式诱导建立化疗性异食癖大鼠模型,观察并记录造模后24 h内大鼠体质量、摄食量和高岭土摄入量的变化。继续干预1 d后,苏木精-伊红(HE)染色观察各组大鼠胃窦、回肠组织形态学变化;实时荧光定量逆转录聚合酶链式反应(RT-qPCR)法检测延髓、回肠组织中5-HT3R、色氨酸羟化酶(TPH)、单胺氧化酶A(MAOA)mRNA表达;Western blot法检测延髓组织中5-HT3R蛋白的表达。结果①与正常对照组比较,模型组大鼠体质量、摄食量降低(P<0.05),高岭土摄入量显著增加(P<0.05);与模型组比较,昂丹司琼组和橘皮竹茹汤低、高剂量组大鼠高岭土摄入量显著降低(P<0.05)。②HE染色显示,与正常对照组比较,模型组胃窦组织上皮细胞受损,固有层腺体排列疏松、紊乱,回肠上皮部分缺失,腺体排列紊乱;与模型组比较,昂丹司琼组和橘皮竹茹汤低、高剂组胃窦和回肠组织病理改变减轻。③RT-qPCR结果显示,与正常对照组比较,模型组大鼠延髓、回肠5-HT3R mRNA表达水平显著升高(P<0.05),回肠MAOA mRNA表达水平显著降低(P<0.05);与模型组比较,橘皮竹茹汤高剂量组大鼠延髓5-HT3R mRNA表达水平明显降低(P<0.05),橘皮竹茹汤低剂量组回肠TPH1 mRNA、橘皮竹茹汤高剂量组延髓TPH2 mRNA表达水平明显降低(P<0.05),昂丹司琼组、橘皮竹茹汤低剂量组、橘皮竹茹汤高剂量组回肠MAOA mRNA表达水平明显升高(P<0.05)。④Western blot结果显示,与正常对照组比较,模型组大鼠延髓5⁃HT3R蛋白表达水平显著升高(P<0.05);与模型组比较,橘皮竹茹汤高剂量组大鼠延髓5-HT3R蛋白表达水平显著降低(P<0.05)。结论橘皮竹茹汤可以通过抑制延髓5-HT3R蛋白和mRNA的表达,调控5-羟色胺合成与代谢相关酶,进而改善化疗导致的恶心呕吐。

关键词：异食癖肿瘤化学疗法恶心呕吐橘皮竹茹汤 5-羟色胺3受体经典名方中药研究

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Seeing Dirac electrons and heavy fermions in new boron nitride monolayers

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Chinese Physics B 2020年第5期29卷 454-458页

作者： Yu-Jiao Kang Yuan-Ping Chen Jia-Ren Yuan Xiao-Hong yan Yue-E xie School of Physics and Optoelectronics Xiangtan UniversityXiangtan 411105China Faculty of Science Jiangsu UniversityZhenjiang 212013China

Most three-dimensional(3D)and two-dimensional(2D)boron nitride(BN)structures are wide-band-gap ***,we propose two BN monolayers having Dirac points and flat bands,*** monolayer is named as 5-7 BN that consists of five-and seven-membered *** other is a Kagome BN made of triangular boron rings and nitrogen *** two structures show not only good dynamic and thermodynamic stabilities but also novel electronic *** 5-7 BN has Dirac points on the Fermi level,indicating that the structure is a typical Dirac *** Kagome BN has double flat bands just below the Fermi level,and thus there are heavy fermions in the *** flat-band-induced ferromagnetism is also *** analyze the origination of the band structures by partial density of states and projection of *** addition,a possible route to experimentally grow the two structures on some suitable substrates such as the PbO2(111)surface and the CdO(111)surface is also discussed,*** research not only extends understanding on the electronic properties of BN structures,but also may expand the applications of BN materials in 2D electronic devices.

关键词： boron nitride Dirac fermion flat band ferromagnetism

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Construction of a telomerase-immortalized porcine tracheal epithelial cell model for swine-origin mycoplasma infection

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Journal of Integrative Agriculture 2022年第2期21卷 504-520页

作者： xie Xing HAO Fei WANG Hai-yan PANG Mao-da GAN Yuan LIU Bei-bei ZHANG Lei WEI yan-na CHEN Rong ZHANG Zhen-zhen BAO Wen-bin BAI Yun SHAO Guo-qing XIONG Qi-yan FENG Zhi-xin Key Laboratory for Veterinary Bio-Product Engineering of Ministry of Agriculture and Rural Affairs Institute of Veterinary MedicineJiangsu Academy of Agricultural SciencesNanjing 210014P.R.China Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology Institute of Food Safety and NutritionJiangsu Academy of Agricultural SciencesNanjing 210014P.R.China College of Animal Science and Technology Yangzhou UniversityYangzhou 225009P.R.China

Primary porcine tracheal epithelial cells(PTECs) are an appropriate model for studying the molecular mechanism of various porcine respiratory diseases, including swine-origin mycoplasmas, which are isolated from respiratory tract of pigs and mainly found on the mucosal surface surrounding swine trachea. However, the short proliferation ability of primary PTECs greatly limits their lifespan. In this study, primary PTECs were carefully isolated and cultured, and immortal PTECs were constructed by transfecting primary PTECs with the recombinant constructed plasmid pEGFPhTERT containing human telomerase reverse transcriptase(hTERT). Immortal PTECs(hTERT-PTECs) maintained both the morphological and functional characteristics of primary PTECs, as indicated by the expression of cytokeratin 18, cellcycle analysis, proliferation assay, Western blotting, telomerase activity assay, karyotype analysis and quantitative RTPCR. Compared to primary PTECs, hTERT-PTECs had an extended replicative lifespan, higher telomerase activity, and enhanced proliferative activity. In addition, this cell line resulted in a lack of transformed and grown tumors in nude mice, suggesting that it could be safely applied in further studies. Moreover, hTERT-PTECs were vulnerable to all swineorigin mycoplasmas through quantitative analysis as indicated by 50% color changing unit(CCU_(50)) calculation, and no significant differences of adhesion ability between primary and immortal PTECs were observed. For the representative swine mycoplasma Mycoplasma hyopneumoniae(Mhp), except for DNA copies quantitative real-time PCR assay, indirect immunofluorescence assay and Western blotting analysis also depicted that hTERT-PTECs was able to adhere to different Mhp strains of different virulence. In summary, like primary PTECs, hTERT-PTECs could be widely used as an adhesion cell model for swine-origin mycoplasmas and in infection studies of various porcine respiratory pathogens.

关键词： porcine tracheal epithelial cells(PTECs) hTERT-PTECs swine-origin mycoplasmas adhesion cell model

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重庆地区油茶低产林高接换种改造成效分析

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南方农业 2024年第9期18卷 137-143页

作者：谢英赞王朝英马立辉李果晏巧张宇重庆市林业科学研究院重庆400036 重庆城市管理职业学院重庆400101 重庆山地型城市森林生态系统国家定位观测研究站重庆400036

为解决重庆地区油茶低产林改造投入高、周期长、成效不稳定等问题,以重庆当地引种驯化的6个优良品种为接穗,通过高接换种方式进行品种改造。结果显示:6个优良品种的高换成活率表现最好的是长林18号,长林4号和长林3号次之;接穗基径粗度... 详细信息

为解决重庆地区油茶低产林改造投入高、周期长、成效不稳定等问题,以重庆当地引种驯化的6个优良品种为接穗,通过高接换种方式进行品种改造。结果显示:6个优良品种的高换成活率表现最好的是长林18号,长林4号和长林3号次之;接穗基径粗度表现最好的是长林18号,长林3号、长林4号和长林40号次之;枝条长度和树木冠幅生长方面,长林4号表现最好,长林3号次之。产量方面,单株鲜果产量大小依次为长林3号>长林4号>湘林210号>长林53号>长林40号>长林18号。表观质量方面,湘林210号在单果质量、果径和鲜果高度上表现最好,但同时具有果皮最厚的缺点,鲜果出籽率相对不高;长林3号、4号和53号在单果质量、果径和鲜果出籽质量指标上较湘林210号相对略差,但这3个品种果皮薄,鲜果出籽率较高。经AHP层次分析法综合评价,得分高低依次为长林3号(9.359)>长林4号(9.204)>长林53号(8.056)>湘林210号(8.046)>长林40号(7.292)>长林18号(5.506)。重庆油茶低效林高接换种改造品种选择中,优先推荐长林3号和4号,为达到高产效果,可优选长林53号和湘林210号作为配置品种。

关键词：油茶低效林高接换种改造成效重庆

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