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microRNA序列和表达模式的多样性

生物化学与生物物理进展 2015年第8期42卷 689-696页

作者：丁素萍刘丽莉黄婷王营严明理袁志栋湖南科技大学生命科学学院湘潭411201 重金属污染土壤生态修复与安全利用湖南省高校重点实验室湘潭411201

microRNA(miRNA)在后生动植物细胞分化、生长发育、正常生理功能的维持、疾病的发生发展和转归中发挥重要功能.通常认为,已知miRNA对应的序列就是存储于miRBase数据库中相应的成熟miRNA序列.然而,人们忽视了miRNA序列长度和碱基的可变性... 详细信息

microRNA(miRNA)在后生动植物细胞分化、生长发育、正常生理功能的维持、疾病的发生发展和转归中发挥重要功能.通常认为,已知miRNA对应的序列就是存储于miRBase数据库中相应的成熟miRNA序列.然而,人们忽视了miRNA序列长度和碱基的可变性,低估了来源于同一miRNA基因、非主要表达的miRNA的多样性及其功能的重要性.由于测序技术的快速发展和广泛使用,人们利用二代测序技术数据发现了miRNA序列和miRNA表达水平的多样性.基于miRNA序列和表达的多样性,本文介绍了miRNA序列5′端、3′端和内部的变异体、miRNA变异体的时空差异性表达和miRNA优势表达臂转换等方面近年来的研究进展.

关键词： miRNA 多样性 isomiR miRNA编辑臂转换

High-energy-density lithium manganese iron phosphate for lithium-ion batteries: Progresses, challenges, and prospects

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Journal of Energy Chemistry 2025年第1期100卷 1-17页

作者： Bokun Zhang Xiaoyun Wang Shuai Wang yan li libo Chen Handong Jiao Zhijing Yu Jiguo Tu State Key Laboratory of Advanced Metallurgy University of Science and Technology Beijing Research Institute for Intelligent Wearable Systems The Hong Kong Polytechnic University Institute of Advanced Structure Technology Beijing Institute of Technology

The soaring demand for smart portable electronics and electric vehicles is propelling the advancements in high-energy–density lithium-ion *** manganese iron phosphate (liMnxFe1-xPO4) has garnered significant attention as a promising positive electrode material for lithium-ion batteries due to its advantages of low cost,high safety,long cycle life,high voltage,good high-temperature performance,and high energy *** liMnxFe1-xPO4has made significant breakthroughs in the past few decades,there are still facing great challenges in poor electronic conductivity and li-ion diffusion,manganese dissolution affecting battery cycling performance,as well as low tap *** review systematically summarizes the reaction mechanisms,various synthesis methods,and electrochemical properties of liMnxFe1-xPO4to analyze reaction processes accurately and guide material ***,the main challenges currently faced are concluded,and the corresponding various modification strategies are discussed to enhance the reaction kinetics and electrochemical performance of liMnxFe1-xPO4,including multi-scale particle regulation,heteroatom doping,surface coating,as well as microscopic morphology ***,in view of the current research challenges faced by intrinsic reaction processes,kinetics,and energy storage applications,the promising research directions are *** importantly,it is expected to provide key insights into the development of high-performance and stable liMnxFe1-xPO4materials,to achieve practical energy storage requirements.

关键词： Semiconductor doping

Hydrogen sulfide reduces oxidative stress in Huntington's disease via Nrf2

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Neural Regeneration Research 2025年第6期20卷 1776-1788页

作者： Zige Jiang Dexiang liu Tingting li Chengcheng Gai Danqing Xin Yijing Zhao yan Song Yahong Cheng Tong li Zhen Wang Department of Physiology School of Basic Medical SciencesCheeloo College of MedicineShandong UniversityJinanShandong ProvinceChina Department of Medical Psychology and Ethics School of Basic Medicine SciencesCheeloo College of MedicineShandong UniversityJinanShandong ProvinceChina Department of Neurosurgery Surgery Qingdao Municipal HospitalQingdaoShandong ProvinceChina

The pathophysiology of Huntington's disease involves high levels of the neurotoxin quinolinic acid. Quinolinic acid accumulation results in oxidative stress, which leads to neurotoxicity. However, the molecular and cellular mechanisms by which quinolinic acid contributes to Huntington's disease pathology remain unknown. In this study, we established in vitro and in vivo models of Huntington's disease by administering quinolinic acid to the PC12 neuronal cell line and the striatum of mice, respectively. We observed a decrease in the levels of hydrogen sulfide in both PC12 cells and mouse serum, which was accompanied by down-regulation of cystathionine β-synthase, an enzyme responsible for hydrogen sulfide production. However, treatment with NaHS(a hydrogen sulfide donor) increased hydrogen sulfide levels in the neurons and in mouse serum, as well as cystathionine β-synthase expression in the neurons and the mouse striatum, while also improving oxidative imbalance and mitochondrial dysfunction in PC12 cells and the mouse striatum. These beneficial effects correlated with upregulation of nuclear factor erythroid 2-related factor 2 expression. Finally, treatment with the nuclear factor erythroid 2-related factor 2inhibitor ML385 reversed the beneficial impact of exogenous hydrogen sulfide on quinolinic acid-induced oxidative stress. Taken together, our findings show that hydrogen sulfide reduces oxidative stress in Huntington's disease by activating nuclear factor erythroid 2-related factor 2,suggesting that hydrogen sulfide is a novel neuroprotective drug candidate for treating patients with Huntington's disease.

关键词： apoptosis cystathionine-β-synthase nuclear factor erythroid 2-related factor 2 Huntington's disease hydrogen sulfide mitochondrion neuroplasticity oxidative stress quinolinic acid reactive oxygen species

我国高校大学生创业实践教育:模式、问题与对策研究

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黑龙江高教研究 2017年第10期35卷 17-20页

作者：黎春燕李伟铭李翠海南大学经济与管理学院海南海口570228

大学生创业实践教育是当前我国创业教育的关键环节。我国高校历经多年探索出多种典型的创业实践教育模式,但当前我国高校创业实践教育仍然存在创业实践活动形式化、创业实践活动覆盖面有限、缺乏必要的专业支持、创业实践平台不足、创... 详细信息

大学生创业实践教育是当前我国创业教育的关键环节。我国高校历经多年探索出多种典型的创业实践教育模式,但当前我国高校创业实践教育仍然存在创业实践活动形式化、创业实践活动覆盖面有限、缺乏必要的专业支持、创业实践平台不足、创业配套措施不健全等问题。我们可以通过明确创业实践教育定位、降低创业实践门槛、整合创新创业活动、建设跨界师资队伍、共建创业实践平台、完善创业配套机制等六个层面来完善我国高校创业实践教育体系。

关键词：大学生创业创业教育实践教育创业实践

Expert consensus on clinical standardized application of high-flow nasal cannula oxygen therapy in adults

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Chinese Medical Journal 2020年第11期133卷 1322-1324页

作者： Jian-Qiao Xu Long-Xiang Su Peng yan Xing-Shuo Hu Ruo-Xuan Wen Kun Xiao Hong-Jun Gu Jin-Gen Xia Bing Sun Qing-Tao Zhou Yu-Chao Dong Jia-lin liu Pin-Hua Pan Hong Luo Qi li li-Qiang Song Si-Cheng Xu yan-ming li Dao-Xin Wang Dan li Qing-Yuan Zhan li-Xin Xie 无 Departm ent of Pulmonary&Critical Care Medicine Chinese People Liberation Army(PLA)General HospitalBeijing 100853China Department of Critical Care Medicine Peking Union Medical College HospitalBeijing 100005China Department of Pulmonary&Critical Care Medicine China and Japan Friendship HospitalBeijing 100029China Department of Pulmonary&Critical Care Medicine Beijing Chaoyang Hospital affiliated to Capital Medical UniversityBeijing 100020China Department of Pulmonary&Critical Care Medicine Peking University Third HospitalBeijing 100191China Department of Respiratory Medicine Changhai HospitalShanghai 200433China Department of Respiratory Medicine Ruijin Hospital Affiliated to the Shanghai Jiao Tong University Medical SchoolShanghai 200025China Department of Respiratory Medicine XiangYa HospitalCentral South UniversityChangshaHunan 410008China Department of Pulmonary&Critical Care Medicine XiangYa Second Hospital of Central South UniversityChangshaHunan 410011China Department of Pulmonary&Critical Care Medicine Xinqiao Hospital of the Third Military Medical UniversityChongqing 400037China Department of Pulmonary&Critical Care Medicine Xijing Hospital of Fourth Military Medical UniversityXi'anShaanxi 710032China Department of Respiratory Medicine The First Affiliated Hospital of Xinjiang Medical UniversityUrumqiXinjiang 830000China Department of Pulmonary&Critical Care Medicine Beijing HospitalBeijing 100730China Department of Respiratory Medicine The Second Affiliated Hospital of Chongqing Medical UniversityChongqing 400010China Department of Respiratory Medicine The First Bethune Hospital of Jilin UniversityChangchunJilin 130021China Pulmonary&Critical Care Medicine Group of Chinese Thoracic Society/Pulmonary&Critical Care Medicine Committee of Chinese Association of Chest Physician

High-flow nasal cannula oxygen therapy(HFNC)is a novel respiratory support technology and has been extensively applied in clinics in recent *** therapeutic equipment mainly includes an air-oxygen blender,an active humidifier,a high-flow nasal cannula,and a connecting inspiratory *** provides patients a humidified high-flow gas(8-80 L/min)with relatively stable oxygen concentration(21-100%),temperature(31-37℃)and performs oxygen therapy via a nasal cannula,which is very comfortable.

关键词： flow connecting high

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3种植物对红壤中镉的富集特性研究

农业环境科学学报 2009年第1期28卷 72-77页

作者：严明理刘丽莉王海华向言词冯涛湖南科技大学生命科学学院湖南湘潭411201

重金属超富集植物是重金属污染土壤植物修复的基础,研究了3种重金属富集植物羽叶鬼针草、美洲商陆和紫叶芥菜对重金属Cd的吸收积累规律,为植物修复Cd污染的农田和生态环境建设提供科学依据。采用盆栽方法,在不同浓度(0、20、35、50、65... 详细信息

重金属超富集植物是重金属污染土壤植物修复的基础,研究了3种重金属富集植物羽叶鬼针草、美洲商陆和紫叶芥菜对重金属Cd的吸收积累规律,为植物修复Cd污染的农田和生态环境建设提供科学依据。采用盆栽方法,在不同浓度(0、20、35、50、65、80mg·kg-1)Cd处理下,分别测定3种植物地上部与根部Cd的含量,计算了地上部Cd迁移量、根系耐性指数、富集系数,研究了土壤中Cd添加量与植物富集Cd量的相关性。结果表明,随着土壤中Cd离子浓度的升高,3种植物地上部和根系中的Cd含量也在增加,相关系数都大于0.99;综合地上部与根部Cd含量,地上部Cd迁移量,根系耐性指数和富集系数,3种植物对Cd的富集能力的相对顺序为:羽叶鬼针草>美洲商陆>紫叶芥菜。羽叶鬼针草、美洲商陆种植在Cd处理浓度为65mg·kg-1的土壤中和紫叶芥菜种植在Cd处理浓度为80mg·kg-1的土壤中栽培时,3种植物地上部与根部的Cd含量均超过了100mg·kg-1,达到了Cd超富集量的标准。羽叶鬼针草、美洲商陆和紫叶芥菜对Cd有很强的耐受性和富集性,可以作为先锋植物去修复被Cd污染的土壤。

关键词：植物镉富集特性红壤

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薏苡仁油微胶囊壁材的选择及工艺优化

中国食品添加剂 2017年第5期28卷 117-123页

作者：闫岩王明力李岑范劲赵婕汤翠贵州大学酿酒与食品工程学院贵阳550025 贵州省发酵工程与生物制药重点实验室贵阳550025 贵州理工学院制药工程学院贵阳550003

选用β-环糊精、变性淀粉HI-CAP100两种碳水化合物和大豆分离蛋白、明胶两种蛋白质类壁材进行组合初步筛选薏苡仁油微胶囊的壁材,再利用单因素试验研究了壁材的配比、芯壁比、乳化液壁材含量、乳化剂添加量对微胶囊包埋率的影响,最后通... 详细信息

选用β-环糊精、变性淀粉HI-CAP100两种碳水化合物和大豆分离蛋白、明胶两种蛋白质类壁材进行组合初步筛选薏苡仁油微胶囊的壁材,再利用单因素试验研究了壁材的配比、芯壁比、乳化液壁材含量、乳化剂添加量对微胶囊包埋率的影响,最后通过L_9(3~4)正交试验确定了壁材的最佳配比。结果表明:大豆分离蛋白/变性淀粉HI-CAP100壁材组合在选用的6组壁材组合中综合表现出了良好的潜质,通过工艺优化得到了微胶囊的最佳配方:大豆分离蛋白占壁材质量分数为5%,芯壁比为60%,壁材含量为30%,乳化剂添加量为1.0%,由此得到的薏苡仁油微胶囊的包埋率为78.52%。

关键词：薏苡仁油微胶囊壁材包埋率

基于Levenberg-Marquardt方法的内蒙古及邻区地震烈度影响场改进技术

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地震 2017年第3期37卷 117-126页

作者：杨彦明姜立新王祯祥内蒙古自治区地震局呼和浩特010010 中国地震台网中心北京100045

在收集和整理内蒙古及邻近地区1940年以来M_S4.0以上历史震害资料的基础上,对研究区域进行合理分区,选取65次中强地震事件的152条等震线数据,利用椭圆烈度衰减模型,采用Levenberg-Marquardt非线性反演方法,建立内蒙古及邻区的分区地震... 详细信息

在收集和整理内蒙古及邻近地区1940年以来M_S4.0以上历史震害资料的基础上,对研究区域进行合理分区,选取65次中强地震事件的152条等震线数据,利用椭圆烈度衰减模型,采用Levenberg-Marquardt非线性反演方法,建立内蒙古及邻区的分区地震烈度衰减关系,通过与地震灾害评估系统使用的华北地区地震影响场模型进行对比分析,综合分析研究认为,建立分区烈度衰减关系更符合研究区域地震灾害的地域性特点,对于地震灾害快速评估具有重要的参考意义。

关键词：烈度地震影响场衰减关系 Levenberg—Marquardt方法内蒙古地区

克氏原螯虾一种新型甲壳素基因的鉴定和抑菌功能研究

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水生生物学报 2022年第2期46卷 248-256页

作者：焦厚琪霍诗天闫黎明李艳和刘学芹华中农业大学水产学院武汉430070 湖北省水生动物病害防控工程技术研究中心武汉430070

抗菌肽是无脊椎动物非特异性免疫系统中重要的免疫因子,抗菌肽具有良好且广谱的抗菌活性,并且不易产生耐药性,有望替代传统抗生素药物发挥功能。甲壳素(Crustin)是目前甲壳类动物中研究比较广泛的一类抗菌肽家族。研究鉴定了来自克氏原... 详细信息

抗菌肽是无脊椎动物非特异性免疫系统中重要的免疫因子,抗菌肽具有良好且广谱的抗菌活性,并且不易产生耐药性,有望替代传统抗生素药物发挥功能。甲壳素(Crustin)是目前甲壳类动物中研究比较广泛的一类抗菌肽家族。研究鉴定了来自克氏原螯虾(Procambarus clarkii)中的一种新型Crustin基因,命名为Pc-CruL。通过Pc-CruL抵抗病原性细菌的免疫防御反应的初步探究,发现Pc-CruL对克氏原螯虾的天然免疫系统至关重要,为深入研究克氏原螯虾奠定了一定的基础,在克氏原螯虾的人工养殖过程中的疾病的预防和治疗中具有一定的指导意义且提供了一定的理论基础。Pc-CruL包含330 bp,编码109个氨基酸,与已知的克氏原螯虾甲壳素同源性较低,为Ⅰ型甲壳素蛋白家族新成员。Pc-CruL在健康的克氏原螯虾的血淋巴、鳃、肠道、胃、心和肝胰腺中均存在,在血淋巴中的含量最高,而在肝胰腺中含量最低。同时进行了体外抑菌实验,结果表明Pc-CruL重组蛋白显著地抑制了几种常见的病原性细菌的生长,具有良好的广谱抑菌活性。体内实验表明,对健康的克氏原螯虾注射Pc-CruL重组蛋白可以保护克氏原螯虾,在副溶血弧菌感染后,提高存活率。Pc-CruL是克氏原螯虾中关键的免疫因子,在克氏原螯虾抵抗外界病原菌入侵和感染过程中起着重要的作用。

关键词：克氏原螯虾抗菌肽先天免疫甲壳素副溶血弧菌

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