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Isolation and application of effective nitrogen fixation rhizobial strains on low-phosphorus acid soils in South China

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Chinese Science Bulletin 2009年第3期54卷 412-420页

作者： CHENG FengXiant CAO GuiQin WANG XiuRong ZHAO Jing yan XiaoLong liao hong Root Biology Center South China Agricultural University Guangzhou 510642 China

Soybean (Glycine max L.) is a very important food and oil crop in China. Legume-rhizobium symbiotic nitrogen (N) fixation is an important biological character and also the base of improving soil fertility of soybean. However, soybean production and development is severely limited in tropical and subtropical areas in China due to a lack of effective rhizobial inoculants adapting to low-phosphorus (P) acid soils. In the present study, 12 soybean rhizobial strains were isolated and purified from the nodules of two soybean genotypes contrasting in P efficiency, which were grown on different low-P acid soils with different soybean cultivation histories. Results from 16S rDNA sequence analysis showed that these 12 rhizobial strains belonged to the genus of Bradyrhizobium, which had higher nitrogenase activities compared to the control strain, Bradyrhizboium japonicum USDA110. A field experiment was carried out by applying rhizobial inoculants, a mixture of three rhizobial strains that showed the highest ni- trogenase activity, on a typical low-P acid soil in South China. The results showed that, without inocu- lation, no nodules were formed in the three soybean genotypes tested; with inoculation, the nodulation rates in all were 100%. Inoculation with rhizobial inoculants not only made many nodules formed, but also increased soybean shoot biomass and yield, and improved nitrogen (N) and P nutrient status. Among which, shoot dry weight, N and P content of a soybean genotype, Huachun 3, inoculated with rhizobium were increased 154.3%, 152.4% and 163.2% compared to that without inoculation, respec- tively. We concluded that: (i) The effective indigenous rhizobial strains isolated in this study from soybeans on low-P acid soils in South China have the characters of broad host range, high nodulation efficiency, efficient N fixation, great low pH and low P tolerance. (ii) Soil environment and host types are the key factors to screen the effective rhizobial strains. Considering

关键词：酸性土壤大豆根瘤菌氮固定

A sutureless technique for securing leaking sclerotomies with viscoelastic substances in 23-gauge microincision vitrectomy surgery

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International Journal of Ophthalmology(English edition) 2023年第5期16卷 730-735页

作者： Meng Li Quan-Yong Yi Jing-Hai Mao yan-hong liao yan-yan Wang Qin-Kang Lu yan Gong Health Science Center Ningbo UniversityNingbo 315211Zhejiang ProvinceChina Department of Ophthalmology Ningbo Eye HospitalNingbo 315042Zhejiang ProvinceChina Department of Ophthalmology The Affiliated People’s Hospital of Ningbo UniversityNingbo 315040Zhejiang ProvinceChina

AIM:To introduce and evaluate the clinical efficacy of a new technique,the use of viscoelastic substances(VS)to close leaking sclerotomy in 23G microincision vitrectomy,and to observe its effect on the visual acuity and intraocular pressure(IOP)of ***:Patients who underwent 23G vitrectomy in Ningbo Eye Hospital before the use of VS technique(June 2019 to September 2020)and after the use of VS technique(October 2020 to December 2021)were selected as the subjects of this *** above cases underwent operation by the same surgeon and were retrospectively *** technique was used as the alternative to suturing,in which a small amount of VS was injected at the leaking sclerotomy and then gently massaged to confirm leaking sclerotomy ***:A total of 174 eyes were covered in the study,including 84 eyes in the control group(before the use of VS technique)and 90 eyes in the VS technique *** number of eyes that needed to be sutured decreased considerably from 42.9%in the control group to 3.3%in the VS technique group,and the proportion of subconjunctival hemorrhage at 1-2d after surgery decreased remarkably from 35.7%in the control group to 2.2%in the VS technique *** substantial differences in the incidence of mean IOP and low IOP were found between 1-2 and 3-20d after surgery in the VS technique *** major complications associated with VS technique were identified during the ***:In 23G microincision vitrectomy,VS technique is a safe,simple,and effective method to close leaking sclerotomy.

关键词： vitrectomy surgery leaking sclerotomy 23-gauge sutureless technique viscoelastic substances intraocular pressure

Effect of nintedanib thermo-sensitive hydrogel on neovascularization in alkali burn rat model

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International Journal of Ophthalmology(English edition) 2020年第6期13卷 879-885页

作者： yan Gong Guo-Hai Wu Ling-Yi Zhang Zhe Zhang yan-hong liao Xiao-Tian Liu Department of Ophthalmology Ningbo Eye HospitalNingbo 315040Zhejiang ProvinceChina

AIM:To investigate the effects of nintedanib thermo-sensitive hydrogel(NTH)on neovascularization and related markers in corneal alkali burns of Wistar ***:NTH was prepared by grinding,and its phase-transition temperature was *** specific-pathogen-free Wistar rats served as a model of corneal alkali burn in the right eye were randomly divided into 3 groups(n=10,each):model group treated with 0.9%saline once a day,NTH group with 0.2%nintedanib b.i.d,and dexamethasone group with dexamethasone ointment once a *** left eye of rats served as the *** corneal transparency was observed under a slit-lamp microscope,and the area of neovascularization was *** day 7,the rats were sacrificed,and the cornea was removed and embedded with paraffin,then stained with hematoxylin一eosin,and the expression of vascular endothelial growth factor receptor 2(VEGFR-2)and CD31 in the corneal tissues of each group was detected by ***:The phase-transition temperature ofnintedanib obtained by grinding was 37℃after adding artificial *** results of the alkali burn model indicated that the growth rate of neovascularization in the NTH group was slower than that in the model group,and the neovascularization area was significantly smaller than that in the model group(P<0.05).Moreover,CD31 and VEGFR-2 expression levels in the NTH group were significantly lower than those in the model ***:NTH becomes colloidal at body temperature,which is beneficial for releasing the drug slowly and can significantly inhibit the neovascularization of corneal induced by alkali burn in rats.

关键词： nintedanib alkali burn neovascularization cornea rat

TRPC6 Knockout Alleviates Renal Fibrosis through PI3K/AKT/GSK3B Pathway

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Current Medical Science 2024年第3期44卷 589-602页

作者： An-bang SUN Fang-hua LI Lin ZHU Xi-xi ZENG Min ZHU Qing-hua LEI yan-hong liao Department of Anatomy School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Transfusion Medicine Wuhan Hospital of Traditional Chinese and Western MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Nephrology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou450052China Department of Thoracic Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China National Basic Medical Teaching Demonstration Center Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Key Laboratory of Neurological Diseases of Ministry of Education Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China

Objective Renal fibrosis is the ultimate pathway of various forms of acute and chronic kidney ***,the knockout of transient receptor potential channel 6(TRPC6)has shown promise in alleviating renal ***,the regulatory impact of TRPC6 on renal fibrosis remains *** In vivo,TRPC6 knockout(TRPC6−/−)mice and age-matched 129 SvEv(WT)mice underwent unilateral renal ischemia-reperfusion(uIR)injury surgery on the left renal pedicle or sham *** and serum were collected on days 7,14,21,and 28 after *** vitro,primary tubular epithelial cells(PTECs)were isolated from TRPC6−/−and WT mice,followed by treatment with transforming growth factorβ1(TGFβ1)for 72 *** anti-fibrotic effect of TRPC6−/−and the underlying mechanisms were assessed through hematoxylin-eosin staining,Masson staining,immunostaining,qRT-PCR,and Western *** Increased TRPC6 expression was observed in uIR mice and PTECs treated with TGFβ***6−/−alleviated renal fibrosis by reducing the expression of fibrotic markers(Col-1,α-SMA,and vimentin),as well as decreasing the apoptosis and inflammation of PTECs during fibrotic progression both in vivo and in ***,we found that the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/glycogen synthase kinase 3 beta(GSK3β)signaling pathway,a pivotal player in renal fibrosis,was down-regulated following TRPC6 *** These results suggest that the ablation of TRPC6 may mitigate renal fibrosis by inhibiting the apoptosis and inflammation of PTECs through down-regulation of the PI3K/AKT/GSK3β*** TRPC6 could be a novel therapeutic strategy for preventing chronic kidney disease.

关键词： transient receptor potential channel 6 ischemia-reperfusion injury renal fibrosis renal tubular epithelial cells

益肾养肝明目方联合抗VEGF药物治疗湿性年龄相关性黄斑变性

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国际眼科杂志 2023年第8期23卷 1362-1366页

作者：高健李萌王彦彦廖燕红龚雁中国浙江省宁波市眼科医院 315040

目的:观察益肾养肝明目方联合抗VEGF药物治疗湿性年龄相关性黄斑变性(wARMD)的临床疗效。方法:选取2020-09/2022-11宁波市眼科医院收治的wARMD患者58例58眼,按照随机数字表法分为注射组(玻璃体腔注射康柏西普,29例29眼)和联合用药组(在... 详细信息

目的:观察益肾养肝明目方联合抗VEGF药物治疗湿性年龄相关性黄斑变性(wARMD)的临床疗效。方法:选取2020-09/2022-11宁波市眼科医院收治的wARMD患者58例58眼,按照随机数字表法分为注射组(玻璃体腔注射康柏西普,29例29眼)和联合用药组(在注射组的基础上口服益肾养肝明目方,29例29眼)。观察两组患者治疗3mo后最佳矫正视力(BCVA)、中心凹厚度(CMT)及中医证候的改善情况,评估临床疗效。结果:治疗3mo后,联合用药组总有效率(76%)高于注射组(66%),两组BCVA均较治疗前改善(P<0.05),CMT均较治疗前降低(P<0.05),且联合用药组治疗前后CMT改善情况优于注射组(-155.93±143.79μmvs-95.36±56.81μm,P<0.05)。治疗3mo后,联合用药组各项中医证候均较治疗前显著好转(P<0.001),注射组仅视物昏蒙较治疗前好转(P<0.05),且联合用药组头晕失眠、腰膝酸软、倦怠乏力、目睛干涩、面白肢冷中医证候积分均显著低于注射组(P<0.001)。结论:益肾养肝明目方联合玻璃体腔注射抗VEGF药物治疗wARMD疗效确切。

关键词：湿性年龄相关性黄斑变性益肾养肝明目方血管内皮生长因子(VEGF) 康柏西普中心凹厚度中医证候

息肉状脉络膜血管病变的光学相干断层扫描及血管成像的观察

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国际眼科杂志 2019年第10期19卷 1805-1807页

作者：廖燕红龚雁陈利双叶婷刘栋中国浙江省宁波市眼科医院

目的:对比观察息肉状脉络膜血管病变(PCV)频域光相干断层成像(OCT)与光相干断层扫描血管成像(OCTA)的图像特征。方法:选取2018-03/12在我院确诊为息肉状脉络膜血管病变(PCV)的患者25例25眼纳入研究,所有患者均行频域OCT和OCTA检查,观察... 详细信息

目的:对比观察息肉状脉络膜血管病变(PCV)频域光相干断层成像(OCT)与光相干断层扫描血管成像(OCTA)的图像特征。方法:选取2018-03/12在我院确诊为息肉状脉络膜血管病变(PCV)的患者25例25眼纳入研究,所有患者均行频域OCT和OCTA检查,观察上述检查结果的图像特征。结果:本研究纳入患者25眼,频域OCT检查表现为浆液性和出血性色素上皮脱离(PED)14眼,OCTA检查示相应病变部位为低信号暗区,其旁有强信号点9眼;频域OCT检查表现为“双层征”11眼,OCTA检查显示为网状结构的脉络膜异常分支血管网(BVN);频域OCT检查表现为息肉样病变(polyps)12眼,OCTA检查表现为强或低信号区。结论:PCV患眼OCTA检查对BVN的表现更直观明显,息肉样病变表现为强或弱的信号,浆液性、出血性PED的OCT和OCTA图像均表现为低反射或低信号暗区,不具有特征性。二者显示的病变位置和形态具有相似性,但也有不同。

关键词：息肉状脉络膜血管病变光学相干断层扫描血管成像图像特征脉络膜分支血管网息肉样病变

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视力骤降待查患者行OCT检查的临床意义

国际眼科杂志 2014年第11期14卷 2105-2107页

作者：刘小天周宏健廖燕红陈辉中国浙江省宁波市眼科医院 315040

目的:探讨在因屈光介质因素而首诊为视力骤降待查的患者中,应用OCT检查来判断视网膜动脉阻塞这类眼科急症的可行性和临床意义。方法:回顾分析我院78例因视力骤降待查接受OCT检查并最终通过FFA检查确诊的病例。以FFA检查作为判断标准,以... 详细信息

目的:探讨在因屈光介质因素而首诊为视力骤降待查的患者中,应用OCT检查来判断视网膜动脉阻塞这类眼科急症的可行性和临床意义。方法:回顾分析我院78例因视力骤降待查接受OCT检查并最终通过FFA检查确诊的病例。以FFA检查作为判断标准,以OCT检查作为待评价的检查手段,并用软件统计OCT的灵敏度、特异度和总符合率。同时统计分析两种检查方法从门诊医生开出检查单到报告给出的用时差异。结果:OCT检查的灵敏度、特异度和总符合率均相当高,分别为100%,94.1%和98.4%。而在从门诊医师开出检查单到报告给出的用时上,OCT检查的时间明显短于FFA,其差异有统计学意义(P=0.000)。结论:临床上对于因屈光介质因素而首诊为视力骤降待查的患者,应首先使用OCT检查来判断是否为视网膜动脉阻塞类疾病,以便更快地收治患者,为抢救视力赢得时间。

关键词：光学相干断层扫描视力骤降急症视网膜动脉阻塞

Human-induced pluripotent stem cell-derived neural stem cell exosomes improve blood-brain barrier function after intracerebral hemorrhage by activating astrocytes via PI3K/AKT/MCP-1 axis

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Neural Regeneration Research 2025年第2期20卷 518-532页

作者： Conglin Wang Fangyuan Cheng Zhaoli Han Bo yan Pan liao Zhenyu Yin Xintong Ge Dai Li Rongrong Zhong Qiang Liu Fanglian Chen Ping Lei Department of Geriatrics Tianjin Medical University General HospitalTianjinChina School of Medicine Nankai UniversityTianjinChina Tianjin Neurological Institute TianjinChina

Cerebral edema caused by blood-brain barrier injury after intracerebral hemorrhage is an important factor leading to poor ***-induced pluripotent stem cell-derived neural stem cell exosomes(hiPSC-NSC-Exos)have shown potential for brain injury repair in central nervous system *** this study,we explored the impact of hiPSC-NSC-Exos on blood-brain barrier preservation and the underlying *** results indicated that intranasal delivery of hiPSC-NSC-Exos mitigated neurological deficits,enhanced blood-brain barrier integrity,and reduced leukocyte infiltration in a mouse model of intracerebral ***,hiPSC-NSC-Exos decreased immune cell infiltration,activated astrocytes,and decreased the secretion of inflammatory cytokines like monocyte chemoattractant protein-1,macrophage inflammatory protein-1α,and tumor necrosis factor-αpost-intracerebral hemorrhage,thereby improving the inflammatory *** sequencing indicated that hiPSC-NSC-Exo activated the PI3K/AKT signaling pathway in astrocytes and decreased monocyte chemoattractant protein-1 secretion,thereby improving blood-brain barrier *** with the PI3K/AKT inhibitor LY294002 or the monocyte chemoattractant protein-1 neutralizing agent C1142 abolished these *** summary,our findings suggest that hiPSC-NSC-Exos maintains blood-brain barrier integrity,in part by downregulating monocyte chemoattractant protein-1 secretion through activation of the PI3K/AKT signaling pathway in astrocytes.

关键词： AKT astrocyte blood-brain barrier cerebral edema exosomes human-induced pluripotent stem cells intracerebral hemorrhage neural stem cells neuroinflammation PI3K

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张涤教授论治小儿肺炎继发腹泻经验撷萃

湖南中医药大学学报 2015年第8期35卷 41-43页

作者：廖艳红张涤湖南中医药大学湖南长沙410208 湖南中医药大学第一附属医院湖南长沙410007

张涤系湖南中医药大学第一附属医院儿科主任医师,教授,硕士研究生导师,知名中医专家,享受国务院政府特殊津贴专家,2009年被评为全国医药卫生系统先进个人。张涤教授从事中医儿科临床工作十余年,熟读中医古典医籍,具有较高的专业理论水平... 详细信息

张涤系湖南中医药大学第一附属医院儿科主任医师,教授,硕士研究生导师,知名中医专家,享受国务院政府特殊津贴专家,2009年被评为全国医药卫生系统先进个人。张涤教授从事中医儿科临床工作十余年,熟读中医古典医籍,具有较高的专业理论水平,临床中注重发挥中医辨证与辨病结合的诊疗特色,辨证施治,病症结合,内外兼治,遣方用药精谨,在治疗小儿呼吸系统、消化系统疾病等方面逐渐形成了自己的特色和优势[1]。

关键词：小儿肺炎继发腹泻抗生素相关性腹泻降气平喘方张涤