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GABA_A Receptor Activity Suppresses the Transition from Interictal to Ictal Epileptiform Discharges in Juvenile Mouse Hippocampus

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Neuroscience Bulletin 2018年第6期34卷 1007-1016页

作者： yan-yan chang Xin-Wei Gong Hai-Qing Gong Pei-Ji Liang Pu-Ming Zhang Qin-Chi Lu Department of Neurology Ren Ji Hospital School of Medicine Shanghai Jiao Tong University School of Biomedical Engineering Shanghai Jiao Tong University

Exploring the transition from inter-ictal to ictal epileptiform discharges(IDs) and how GABAAreceptormediated action affects the onset of IDs will enrich our understanding of epileptogenesis and epilepsy *** used Mg2+-free artificial cerebrospinal fluid(ACSF) to induce epileptiform discharges in juvenile mouse hippocampal slices and used a micro-electrode array to record the discharges. After the slices were exposed to Mg2+-free ACSF for 10 min–20 min, synchronous recurrent seizurelike events were recorded across the slices, and each event evolved from inter-ictal epileptiform discharges(IIDs) to pre-ictal epileptiform discharges(PIDs), and then to *** the transition from IIDs to PIDs, the duration of discharges increased and the inter-discharge interval decreased. After adding 3 lmol/L of the GABAAreceptor agonist muscimol, PIDs and IDs disappeared, and IIDs remained. Further, the application of 10 lmol/L muscimol abolished all the epileptiform discharges. When the GABAAreceptor antagonist bicuculline was applied at 10 lmol/L, IIDs and PIDs disappeared, and IDs remained at decreased intervals. These results indicated that there are dynamic changes in the hippocampal network preceding the onset of IDs, and GABAAreceptor activity suppresses the transition from IIDs to IDs in juvenile mouse hippocampus.

关键词： Epileptiform discharge Gamma-aminobutyric acid Bicuculline Muscimol Micro-electrode array Hippocampal slice

Effects of aspirin on metastasis-associated gene expression detected by cDNA microarray

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Acta Pharmacologica Sinica 2004年第10期 87-93页

作者： Xue-qin GAO, Jin-xiang HAN, Hai-yan HUANG, Shi yan, chang-zheng SONG, Hai-nan HUANGKey Laboratory of Ministry of Health for Biotech-Drug, Shandong Medicinal Biotechnology Center, Shandong Academy of Medical Sciences, Ji-nan 250062, ChinaNow in Medical College of Shandong University, Ji-nan 250012, China.

AIM: To investigate the effect of aspirin on the metastasis-associated gene expression in 3AO ovarian cancer cells. METHODS: 3AO cells were treated with aspirin at the concentration of 1.2 mmol/L for 16 and 48 h, respectively. The total RNA was extracted with Trizol reagents and reverse transcribed with Superscript Ⅱand hybridized with cDNA microarray (containing oncogenes, tumor suppressor genes, signal transduction pathway molecules , adhesive molecules, growth factors and ESTs) fabricated in our lab. After normalization, the ratio of gene expression of aspirin treated to untreated 3AO cells being either 2 fold up higher or 0.5 fold down (lower) were defined as differential expression. Semi-quantitative RT-PCR was used to validate the microarray results. RESULTS: Among the 447 metastasis-associated genes, 4 genes were up-regulated and 14 genes were down-regulated in 3AO cells treated with aspirin for 16 h compared with untreated cells. While 24 genes were up-regulated and 10 genes were down-regulated

关键词： aspirin ovarian neoplasms gene expression neoplasm metastasis cDNA microarray

高效液相色谱-串联质谱法测定牛羊组织中苯酚类和水杨酸苯胺类抗蠕虫药多残留

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分析化学 2014年第3期42卷 423-428页

作者：李帅鹏黄显会王伟严常燕孔祥凯华南农业大学国家兽药残留基准实验室

建立了高效液相色谱-串联质谱法同时测定牛、羊组织中硝碘酚腈、氯羟柳胺、氯氰碘柳胺、碘醚柳胺4种苯酚类和水杨酸苯胺类抗蠕虫药的多残留检测方法。样品用乙腈-丙酮（60∶40,V/V）(肌肉、肝脏和肾脏组织)或1%三乙胺乙腈（脂肪组织）... 详细信息

建立了高效液相色谱-串联质谱法同时测定牛、羊组织中硝碘酚腈、氯羟柳胺、氯氰碘柳胺、碘醚柳胺4种苯酚类和水杨酸苯胺类抗蠕虫药的多残留检测方法。样品用乙腈-丙酮（60∶40,V/V）(肌肉、肝脏和肾脏组织)或1%三乙胺乙腈（脂肪组织）超声提取,MAX阴离子固相萃取柱净化,液相色谱分离,采用多反应监测负离子模式对4种药物同时进行定性定量分析,在1;00μg/L范围内,4种药物与其对应峰面积的线性关系良好,相关系数均大于0.99。在牛、羊组织中的检出限（LOD）均为1μg/kg,定量限（LOQ）均为2.5μg/kg。在LOQ、0.5倍高残留限量（MRL）、MRL、2倍MRL添加水平下,4种药物的平均回收率在71%;12%之间,日内相对标准偏差（RSD）在1.1%;4.0%之间,日间RSD在6.4%;4.7%之间。采用本方法对市场上动物可食性组织进行抽样检测,共抽检40份样品,2份样品检测出苯酚类和水杨酸苯胺类抗蠕虫药残留。

关键词：苯酚类和水杨酸苯胺类抗蠕虫药牛羊组织多残留高效液相色谱-串联质谱

阿尔泰地区巴泽雷克文化马鞍毛毡贴花艺术研究

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丝绸 2025年第1期62卷 101页

作者：常艳西安外国语大学艺术遗产研究中心西安710128

针对阿尔泰地区巴泽雷克文化马鞍毛毡贴花艺术,文章采用文献分析、图像分析、类型与历史比较的研究方法,对遗存情况、贴花工艺、历史溯源及盛行的原因、文化信仰几个方面进行梳理和分析。归纳巴泽雷克文化马鞍毛毡及其贴花工艺的制作流... 详细信息

针对阿尔泰地区巴泽雷克文化马鞍毛毡贴花艺术,文章采用文献分析、图像分析、类型与历史比较的研究方法,对遗存情况、贴花工艺、历史溯源及盛行的原因、文化信仰几个方面进行梳理和分析。归纳巴泽雷克文化马鞍毛毡及其贴花工艺的制作流程,探讨其用色的工艺和风格。研究认为,这种艺术形式经历了从本土到融合外来艺术样式,再到新本土风格形成的几个发展阶段。其显著特色是多种材料的搭配使用、强烈的冷暖对比配色及独特的动物贴花装饰。同时,毛毡贴花艺术不仅作为生活物资,更是原始宗教信仰的载体。无论从工艺水准还是地域特色,都是同时期甚至更晚时期其他地域无法相比的,因此具有重要的史料价值和艺术价值。

关键词：阿尔泰巴泽雷克文化马鞍毛毡贴花艺术

显微拉曼光谱技术对射击残留物的有机颗粒研究

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光谱学与光谱分析 2017年第1期37卷 114-119页

作者：秦真科杨飞宇刘文斌邹芸严岩王长亮蔡能斌上海市刑事科学技术研究院上海市现场物证重点实验室上海200083 上海市公安局物证鉴定中心上海200083

为射击残留物检测提供了一个快速、简便、准确的无损检测方法,通过激光显微拉曼光谱技术对弹药中的发射药及射击残留物中的有机颗粒进行了研究。研究发现,检测射击残留物有机颗粒获得最优拉曼信号的激发波长为473nm,可有效避开荧光干扰... 详细信息

为射击残留物检测提供了一个快速、简便、准确的无损检测方法,通过激光显微拉曼光谱技术对弹药中的发射药及射击残留物中的有机颗粒进行了研究。研究发现,检测射击残留物有机颗粒获得最优拉曼信号的激发波长为473nm,可有效避开荧光干扰。射击残留物有机颗粒主要来自部分燃烧的发射药颗粒,拉曼光谱证明其主要成分与发射药主要成分基本一致,但部分拉曼谱峰强度降低、消失或是线宽变宽,证明其物质结构也在一定程度上受到了破坏。通过微观形态观察,射击残留物有机颗粒表面以铜黄色、灰黑色为主,表面分布熔坑及附着带有金属光泽的球形无机颗粒,这些特有形貌可视为射击残留物的典型特征,成为射击残留物的重要判据。

关键词：有机颗粒微观形态拉曼光谱荧光光谱

Primary Studies on Burnup Estimation of Irradiated Uranium by Dtermining 236U/238U in Contaminated Environmental Samples JIANG Xiao-yan,ZHANG Ji-long,ZOU Wei,chang Zhi-yuan

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Primary Studies on Burnup Estimation of Irradiated Uranium b...

Based on non-targeted metabolomics for differential components screening of Rosae Chinensis Flos and Rosae Rugosae Flos and their quality evaluation

作者： JIANG Xiao-yan ZHANG Ji-long ZOU Wei chang Zhi-yuan

U/U of the environmental sample can indicate the existence of uranium irradiation,and even can be uesd to estimate the burnup of nuclear spent fuel.A study on the burnup estimation of irradiated uranium which was mixed with natural uranium was carried out by measuring the U/U and U/U in the samples by using MC-ICP-MS. According to the equation(1) and(2),a programme for calculating the burnup of the irradiated

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Traditional Medicine Research 2025年第2期10卷 1-15页

作者： Xu Liang Ni-Hui Zhang Zhi-Lai Zhan Guang-Lu chang yan Gao Xia Li Wen-Yuan Gao School of Pharmaceutical Science and Technology Faculty of MedicineTianjin UniversityTianjin 300193China State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs China Academy of Chinese Medical SciencesBeijing 100700China Key Laboratory of Modern Chinese Medicine Resources Research Enterprises Tianjin 300402China

Background:Rosa chinensis *** Rosa rugosa *** not only of ornamental value,but also edible flowers and the flower buds have been listed in the Chinese Pharmacopoeia as traditional *** two plants have some differences in efficacy,but the flower buds are easily confused for similar *** addition,large-scale cultivation of ornamental rose flowers may lead to a decrease in the effective components of medicinal ***,it is necessary to study the chemical composition and make quality evaluation of Rosae Chinensis Flos(Yueji)and Rosae Rugosae Flos(Meigui).Methods:In this study,40 batches of samples including Meigui and Yueji from different regions in China were collected to establish high-performance liquid chromatography ***,the fingerprints data was analyzed using principal component analysis,hierarchical cluster analysis,and partial least squares discriminant analysis analysis chemometrics to obtain information on intergroup differences,and non-targeted metabolomic techniques were applied to identify and compare chemical compositions of samples which were chosen from groups with large *** compounds were screened by orthogonal partial least-squares discriminant analysis and S-plot,and finally multi-component quantification was performed to comprehensively evaluate the quality of Yueji and ***:The similarity between the fingerprints of 40 batches roses and the reference print R was 0.73 to 0.93,indicating that there were similarities and differences between the *** principal component analysis and hierarchical cluster analysis of fingerprints data,the samples from different origins and varieties were intuitively divided into four *** least-squares discriminant analysis analysis showed that Meigui and Yueji cluster into two categories and the model was reliable.A total of 89 compounds were identified by high resolution mass spectrometry,mainly were flavonoids and flavon

关键词： Rosa chinensis Jacq. Rosa rugosa Thunb. metabolomics chemometrics multiple component quantification quality evaluation

SnAgCu无铅钎料对接接头时效过程中IMC的生长

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上海交通大学学报 2007年第S2期41卷 66-70页

作者：李凤辉李晓延严永长北京工业大学材料科学与工程学院北京100022

无铅钎料和铜基板间金属间化合物(Intermetallic Compounds,IMC)的生长对元器件的可靠性有重要影响.使用Sn3.8Ag0.7Cu无铅钎料焊接Cu对接接头,并对对接头进行了125、150和175℃时效试验,时效时间分别为0、24、72、144、256、400 h.采用... 详细信息

无铅钎料和铜基板间金属间化合物(Intermetallic Compounds,IMC)的生长对元器件的可靠性有重要影响.使用Sn3.8Ag0.7Cu无铅钎料焊接Cu对接接头,并对对接头进行了125、150和175℃时效试验,时效时间分别为0、24、72、144、256、400 h.采用金相显微镜、扫描电镜(SEM)和能谱X射线(EDX)观察了Sn3.8Ag0.7Cu/Cu界面IMC的生长及形貌变化,并对Sn3.8Ag0.7Cu/Cu界面扩散常数和生长激活能进行了拟合.此外,研究了时效对钎焊接头抗拉强度的影响,发现在时效条件下接头的抗拉强度呈先上升后下降的变化,且时效会对断裂形式造成影响.

关键词：无铅钎料金属间化合物钎焊时效

外源转基因片段从转基因棉籽壳到食用菌水平转移研究

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食品与机械 2018年第2期34卷 1-5页

作者：白亚龙索玉娟林婷沈源源赵晓燕刘海燕周昌艳上海市农业科学院农产品质量标准与检测技术研究所上海201403

研究利用转基因棉籽壳种植食用菌是否会导致外源转基因片段从棉籽壳水平转移到食用菌中。从银耳生产基地与菇厂采集15份银耳样品与10份平菇样品(培养基质中转基因棉籽壳含量分别约为75%与92%),用PCR扩增检测是否存在转基因棉籽壳中的外... 详细信息

研究利用转基因棉籽壳种植食用菌是否会导致外源转基因片段从棉籽壳水平转移到食用菌中。从银耳生产基地与菇厂采集15份银耳样品与10份平菇样品(培养基质中转基因棉籽壳含量分别约为75%与92%),用PCR扩增检测是否存在转基因棉籽壳中的外源片段CaMV 35S、NOS、CpTⅠ、Cry IA(c)。此外,针对可能会用含棉籽壳基质培养的市售食用菌,从上海市闵行区各大中型超市与农贸市场采集市售样品47份,用PCR扩增检测外源转基因片段。结果显示,不论是生产基地明确用转基因棉籽壳培养的银耳与平菇样品,还是市售的各类食用菌均未发现存在外源转基因片段向食用菌水平转移的现象。

关键词：转基因棉籽壳银耳平菇食用菌基因水平转移 PCR