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金黄色葡萄球菌型乳房炎奶牛乳腺组织的lncRNA差异表达分析

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生物技术通报 2024年第8期40卷 320-328页

作者：周冉王兴平李彦霞罗仍卓么宁夏大学动物科技学院银川750021

【目的】长链非编码RNA(lncRNA)可参与机体炎症反应,但其在奶牛乳房炎中的表达模式及分子调控机制仍不完全清楚。分析金黄色葡萄球菌(Staphylococcus aureus,***)型乳房炎奶牛乳腺组织中的差异表达lncRNA(DElncRNA)及其功能,为后续深入... 详细信息

【目的】长链非编码RNA(lncRNA)可参与机体炎症反应,但其在奶牛乳房炎中的表达模式及分子调控机制仍不完全清楚。分析金黄色葡萄球菌(Staphylococcus aureus,***)型乳房炎奶牛乳腺组织中的差异表达lncRNA(DElncRNA)及其功能,为后续深入研究提供支撑。【方法】利用转录组测序(RNA-Seq)技术和生物信息学方法对健康奶牛和***诱导乳房炎的奶牛乳腺组织进行lncRNA测序、DElncRNA分析及GO和KEGG功能富集分析。【结果】在两组乳腺组织中共检测到1012个lncRNA。相比于健康奶牛组,***诱导组筛选到75个表达上调的和114个表达下调的lncRNA。GO和KEGG富集分析结果表明,上述DElncRNA可通过免疫相关信号通路而调节奶牛乳房炎症。进一步分析发现,DElncRNA TCONS_00042123、TCONS_00068055、TCONS_00108420和TCONS_00076361可能参与MAPK、NLR、Jak-STAT和TLR信号通路,进而调节***型奶牛乳房炎的发生与发展。【结论】在***诱导炎症的奶牛乳腺组织中获得了189个DElncRNA,可能通过潜在靶基因调控***型奶牛乳房炎的发生与发展过程。

关键词：奶牛乳房炎 lncRNA 差异表达分析金黄色葡萄球菌

基于代谢组学结合生物信息学探讨热毒宁注射液与青霉素注射液联用致类过敏反应的潜在致敏原及作用机制

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药学学报 2024年第2期59卷 382-394页

作者：陈毓龙翟优王晓艳李伟霞张辉吴娅丽杨柳青陈小菲张书琦牛璐冯科冉李琨唐进法张明亮河南中医药大学河南郑州450046 河南省中药临床应用评价与转化工程研究中心河南省中药临床药学中医药重点实验室河南省中药安全评价与风险防控工程研究中心河南中医药大学第一附属医院药学部河南郑州450000 河南中医药大学呼吸疾病中药防治省部共建协同创新中心河南郑州450046

本研究基于代谢组学结合生物信息学的策略分析热毒宁注射液与青霉素注射液联用引发类过敏反应(pseudo-allergic reactions,PARs)的潜在致敏原及作用机制。所有动物实验操作和福利均遵循河南中医药大学第一附属实验动物伦理与动物福利委... 详细信息

本研究基于代谢组学结合生物信息学的策略分析热毒宁注射液与青霉素注射液联用引发类过敏反应(pseudo-allergic reactions,PARs)的潜在致敏原及作用机制。所有动物实验操作和福利均遵循河南中医药大学第一附属实验动物伦理与动物福利委员会要求(批号:YFYDW2020002)。基于UPLC-Q-TOF/MS技术结合UNIFI软件在热毒宁与青霉素混合注射液中共鉴定得到21种化合物。进一步基于分子对接技术筛选出其10种与类过敏原受体MrgprX2激动剂位点具有强烈结合活性的潜在致敏原。采用UPLC-Q-TOF/MS技术进行代谢组学分析发现花生四烯酸、磷脂酰胆碱、磷脂酰丝氨酸、前列腺素类以及白三烯类等34个内源性代谢物为热毒宁注射液与青霉素注射液联用引发PARs的差异代谢物。通过对“潜在致敏原-靶标-差异代谢物”交互网络分析获得二者联用时药液中的绿原酸类(绿原酸、新绿原酸、隐绿原酸、异绿原酸A等)以及β-内酰胺类致敏原可能主要通过调控PLD1、PLA2G12A和CYP1A1这三种上游信号靶标,主要激活花生四烯酸代谢通路,促使肥大细胞的脱颗粒,释放下游的内源性炎性介质,继而诱发PARs。

关键词：中西药联用类过敏反应热毒宁注射液注射用青霉素钠代谢组学生物信息学

Magnetic resonance imaging evaluation of the effects of closed reduction in infants with developmental dysplasia of the hip

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Chinese Medical Journal 2021年第5期134卷 611-613页

作者： mi-Fang li Jing Zhao xiao-long Tan ling-yan Zhang Department of Medical Imaging The Third Affiliated HospitalSouthern Medical UniversityGuangzhouGuangdong 510000China

To the Editor:Manual closed reduction has become the most common treatment for infants with developmental dysplasia of the hip(DDH),*** under 18 months old,because it can minimize the limitations of the post-operative range of motion of the hip joint and reduce iatrogenic avascular necrosis.[1] As 90%of children with symptomatic DDH have lesions on the acetabular labrum,we initiated a magnetic resonance imaging(MRI)study of acetabular labrums of dysplastic hips to determine how magnetic resonance-based parameters and contents of joint space could be used to evaluate the therapeutic effect of closed reduction in DDH patients.

关键词： dysplasia operative resonance

Effects of CO_2 enrichment and spikelet removal on rice quality under open-air field conditions

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Journal of Integrative Agriculture 2016年第9期15卷 2012-2022页

作者： JING li-quan WU yan-zhen ZHUANG Shi-teng WANG yun-xia ZHU Jian-guo WANG Yu-long yanG lian-xin Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou University College of Environmental Science and Engineering Yangzhou University State Key Laboratory of Soil and Sustainable Agriculture/Institute of Soil Science Chinese Academy of Sciences

The increase of atmospheric carbon dioxide（CO_2） concentration adversely affect several quality traits of rice grains, but the biochemical mechanism remains unclear. The objectives of this study were to determine how changes in the source-sink relationship affected rice quality. Source-sink manipulation was achieved by free-air CO_2 enrichment from tillering to maturity and partial removal of spikelet at anthesis using a japonica rice cultivar Wuyunjing 23. Enrichment with CO_2 decreased the head rice percentage and protein concentration of milled rice, but increased the grain chalkiness. In contrast, spikelet removal resulted in a dramatic increase in the head rice percentage and protein concentration, and much less grain chalkiness. Neither CO_2 enrichment nor spikelet removal affected the starch content, but the distribution of starch granule size showed distinct treatment effects. O n average, spikelet removal decreased the percentage of starch granules of diameter 〉10 and 5–10 μm by 23.6 and 5.6%, respectively, and increased those with a diameter of 2–5 and 〈2 μm by 4.6 and 3.3%, respectively. In contrast, CO_2 elevation showed an opposite response： increasing the proportion of large starch granules（〉5 μm） and decreasing that of 〈5 μm. The starch pasting properties were affected by spikelet removal much more than by CO_2 elevation. These results indicated that the protein concentration and starch granule size played a role in chalkiness formation under these experimental conditions.

关键词： rice free-air CO_2 enrichment sink removal quality starch granule size

物料动态安息角对矿用自卸车厢斗结构影响规律

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科学技术与工程 2024年第5期24卷 2099-2104页

作者：杨国彪王朝华王志霞赵炎龙马立峰太原科技大学机械工程学院太原030024

针对矿用自卸车行驶过程中的厢斗内物料稳定性问题,研究厢斗内物料动态安息角对厢斗结构设计的影响。首先,基于离散元方法,分析煤颗粒在自然状态下的安息角;进一步,建立厢斗简化模型,研究不同安装角度下矿车正常行驶及爬坡过程中物料的... 详细信息

针对矿用自卸车行驶过程中的厢斗内物料稳定性问题,研究厢斗内物料动态安息角对厢斗结构设计的影响。首先,基于离散元方法,分析煤颗粒在自然状态下的安息角;进一步,建立厢斗简化模型,研究不同安装角度下矿车正常行驶及爬坡过程中物料的动态安息角,并分析物料滑动规律;结果表明:在水平路面行驶工况下物料动态安息角随厢斗安装角度增加而减小,而爬坡过程中由于厢斗内物料的滑动导致动态安息角随安装角度的增加先缓慢减小再变大再快速减小;当安装角度为10°时可有效避免厢斗内物料的滑动;最后,以确保厢斗稳定性装载及不撒料为目标,提出厢斗侧板后端的角度设计公式,为厢斗结构设计提供理论依据。

关键词：矿用自卸车厢斗安息角安装角度结构设计

双胎早产儿支气管肺发育不良危险因素分析:一项多中心研究

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中国当代儿科杂志 2024年第6期26卷 611-618页

作者：樊雨薇张伊佳温和梅晏红沈蔚丁月琴龙运峰张志钢李桂芳姜泓饶红萍邱建武魏贤张亚昱曾纪斌赵常亮许伟鹏王凡员丽杨秀芳李薇林霓阳陈倩夏昌顺钟鑫琪崔其亮广州医科大学附属第三医院新生儿科/广东省产科重大疾病重点实验室/广东省妇产疾病临床医学研究中心/粤港澳母胎医学高校联合实验室广东广州510150 北京大学第三医院儿科北京100191 四川锦欣妇女儿童医院新生儿科四川成都610011 贵州医科大学附属医院新生儿科贵州贵阳550001 厦门大学附属妇女儿童医院新生儿科福建厦门361003 南方医科大学附属东莞医院新生儿科广东东莞523000 邵阳学院附属第一医院新生儿科湖南邵阳422000 广东省第二人民医院新生儿科广东广州511000 沧州市人民医院新生儿科河北沧州061000 延安大学附属医院新生儿科陕西延安716000 惠州市中心人民医院新生儿科广东惠州516001 汕头大学医学院附属粤北人民医院新生儿科广东韶关512026 武汉科技大学附属孝感医院新生儿科湖北孝感432000 内蒙古医科大学附属医院新生儿科内蒙古呼和浩特010050 汕头大学医学院第二附属医院新生儿科广东汕头515041 包钢集团第三职工医院新生儿科内蒙古包头014010 暨南大学附属第一医院新生儿科广东广州510630 兰州大学第二医院甘肃兰州730000 新乡市中心医院新生儿科河南新乡453000 中山市人民医院新生儿科广东中山528400 东莞市滨海湾中心医院新生儿科广东东莞523808 汕头大学医学院第一附属医院新生儿科广东汕头515041

目的分析胎龄25%是双胎中仅一胎患BPD的危险因素(OR=3.370,95%CI:1.500~7.568,P<0.05),胎龄大是双胎均患BPD的保护因素(P<0.05)。对双胎中仅一胎为BPD组进行组内条件logistic回归分析,提示小于胎龄儿是双胎内个体发生BPD的危险因素(OR=... 详细信息

目的分析胎龄25%是双胎中仅一胎患BPD的危险因素(OR=3.370,95%CI:1.500~7.568,P<0.05),胎龄大是双胎均患BPD的保护因素(P<0.05)。对双胎中仅一胎为BPD组进行组内条件logistic回归分析,提示小于胎龄儿是双胎内个体发生BPD的危险因素(OR=5.017,95%CI:1.040~24.190,P<0.05)。结论双胎早产儿BPD的发生不仅与胎龄相关,也与双胎出生体重差异、小于胎龄儿密切相关。

关键词：支气管肺发育不良危险因素多中心研究双胎儿早产儿

Nitrogen-rich metal-organic framework mediated Cu-N-C composite catalysts for the electrochemical reduction of CO_(2)

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Journal of Energy Chemistry 2021年第3期30卷 555-563页

作者： Si-min Cao Hua-Bo Chen Bao-xia Dong Qiu-Hui Zheng yan-xia Ding Meng-Jie liu She-liang Qian yun-Lei Teng Zong-Wei li Wen-long liu School of Chemistry and Chemical Engineering Yangzhou UniversityYangzhou 225002ZhejiangChina

Cu-based MOFs,i.e.,HKUST-1,etc.,have been pertinently chosen as the pristine materials for CO_(2) ER due to the unique ability of copper for generation hydrocarbon ***,the limited conductivity and stability become the stumbling-block that prevents the development of *** exploring of MOFsderived M-C materials starts a new chapter for the MOFs precursors,which provides a remarkable electronic connection between carbon matrix and metals/metal oxides.N-doped M-N-C with extensive M-N sites scattering into the carbon matrix are more popular because of their impressive contribution to catalytic activity and specific product ***,Cu-N-C system remained undeveloped up to *** lack of ideal precursor,the sensitivity of Cu to be oxidized,and the difficulties in the synthesis of small size Cu nanoparticles are thus known as the main barriers to the development of Cu-N-C ***,a nitrogen-rich Cu-BTT MOF is employed for the derivation of N-doped Cu-N-CT composite electrocatalysts by the pyrolyze ***-temperature pyrolysis product of Cu-N-C1100exhibits the best catalytic activity for productions of CO(-0.6 V ***,jCO=0.4 mA/cm^(2))and HCOOH(-0.9 V ***,jHCOOH=1.4 mA/cm^(2)).

关键词： Carbon mono-oxide Formic acid Metal-organic framework Nitrogen-rich Copper nanoparticle Catalyst

Bmi, WC, WHtR, VFI and BFI: Which Indictor is the Most Efficient Screening Index on Type 2 Diabetes in Chinese Community Population

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Biomedical and Environmental Sciences 2013年第6期26卷 485-491页

作者： mi Sheng Quan YIN Peng HU Nan li Jian hong CHEN xiao Rong CHEN Bo yan liu xia ZHAO Wen Hua College of Arts and Science of Beijing Union University National Center for Chronic and Non-communicable Disease Control and Prevention of China CDC Chinese Center for Disease Control and Prevention

Objective Obesity is a major risk factor for type 2 diabetes, many indexes can be used to describes obesity and predict diabetes. This research attempts to identify the best indicator of obesity to screening diabetes in Chinese population. Methods A cross-sectional data of 8121 subjects aged 35-60 years were included in this research belongs to the Diabetes Appropriate Technology Intervention Study. Anthropometric indicators including body weight, height, waist circumferences （WC）, body fat index （BFI） and visceral fat index （VFI） and blood biochemical indicators after an overnight fast [fasting blood glucose, total cholesterol, high-density lipoprotein （HDL） cholesterol, and triacylglycerol] were measured. Bmi （body mass index） and Weight to Height Ratio was calculated. Results Subjects with obesity had a higher risk of physician diagnosed diabetes （0R=2.50, 95% CI 1.83-3.43）, new diagnosed diabetes （0R=4.23, 95% Cl 2.91-6.15） and pre-diabetes （0R=1.75, 95% CI 1.31-2.34） compared to those with normal Body mass index （Bmi）. There was a significant trend of increased risk of all diabetes status with increased waist circumference （WC）. The waist-to-height ratio （WHtR） yielded the most significant association with new diagnosed diabetes and physician diagnosed diabetes than other indices. Conclusion Central obesity is significantly correlated with diabetes. VFI was most correlated with pre-diabetes while WHtR is an efficient screening index than Bmi and WC in Chinese community diabetes screening.

关键词： Obesity Diabetes Bmi Waist Circumference Waist-to-height ratio

积石山地震诱发金田-草滩村滑坡-泥流灾害链过程与成因

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兰州大学学报（自然科学版） 2024年第1期60卷 1-5页

作者：李亚军岳东霞陈冠张毅郭富赟赵岩祁天君曾润强孟兴民兰州大学地质科学与矿产资源学院甘肃省环境地质与灾害防治技术创新中心兰州730000 兰州大学资源环境学院兰州730000 甘肃省地质环境监测院兰州730050

2023年12月18日23:59在甘肃省积石山保安族东乡族撒拉族自治县发生6.2级地震,地震在青海省民和回族土族自治县中川乡金田及草滩村引发了一处滑坡-泥流灾害链事件.利用无人机摄影测量、激光雷达扫描和电阻率层析成像等技术,结合详细现场... 详细信息

2023年12月18日23:59在甘肃省积石山保安族东乡族撒拉族自治县发生6.2级地震,地震在青海省民和回族土族自治县中川乡金田及草滩村引发了一处滑坡-泥流灾害链事件.利用无人机摄影测量、激光雷达扫描和电阻率层析成像等技术,结合详细现场调查及访问对该灾害链事件展开勘查分析,发现地震诱发台塬面下饱水黄土层液化是本次灾害链的主要原因.该饱水黄土层的形成与区域长期的农业灌溉有关,冬季地下水出口被冻结导致的地下水位上涨增加了液化的可能性.黄河上游类似中川乡地质地貌条件的引水灌溉区域有很多,未来地震条件下发生区域液化滑坡-泥流灾害链的风险性极高.

关键词：积石山地震液化灌溉滑坡-泥流灾害链