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GAMYB transcription factor LoMYB65 from lily plays a vital role in pollen development

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Horticultural Plant Journal 2024年第1期10卷 223-238页

作者： Xinyue Liu ling He Ze wu Nianjun Teng Key Laboratory of Landscaping Agriculture Ministry of Agriculture and Rural AffairsCollege of HorticultureNanjing Agricultural UniversityNanjingJiangsu 210095China Key Laboratory of Biology of Ornamental Plants in East China National Forestry and Grassland AdministrationCollege of HorticultureNanjing Agricultural UniversityNanjingJiangsu 210095China Jiangsu Graduate Workstation of Nanjing Agricultural University and Nanjing Oriole Island Modern Agricultural Development Co. Ltd.NanjingJiangsu 210043China

Lily(Lilium spp.)is an important horticultural crop,but its use is limited due to serious pollen contamination *** are many studies on pollen development in model plants,but few on flower crops such as ***(GA)is a large class of hormones and plays an important role in plant vegetative growth and reproductive *** is a group of the R2R3-MYB family upregulated by gibberellin,and plays an important role in anther ***,we isolated a novel GAMYB,named LoMYB65,from lily,which was closely related to the AtMYB65 and AtMYB33 in *** quantitative PCR results showed that LoMYB65 was mainly expressed in lily ***65 could be activated by 288μmol·L^(-1)GA3treatment and the LoMYB65 protein was located in the nucleus and cytoplasm,and had transactivation in yeast and tobacco leaf *** conserved motif within 226 amino acids of the C-terminal of LoMYB65 contributed to its *** of LoMYB65 caused dwarf phenotype,unnormal tapetum development,less seeds of siliques in transgenic Arabidopsis plants,the transgenic plants showed partly male ***,silencing of LoMYB65 with VIGS(Virus Induced Gene Silencing)in lily anthers caused unnormal pollen development and reduced the pollen *** of LoMYB65 in Arabidopsis and silencing of LoMYB65 in lily resulted in decreased pollen counts,so we speculate that LoMYB65 may be ***,these findings suggest that LoMYB65 may play an important role in anther development and pollen formation in ***65 may provide a useful candidate gene for pollenless breeding of lily.

关键词： Lily Anther development Pollen pollution GAMYB VIGS

Optimal proportioning of iron ore in sintering process based on improved multi-objective beluga whale optimisation algorithm

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Journal of Iron and Steel Research International 2024年第7期31卷 1597-1609页

作者： Zong-ping Li Xu-dong Li Xue-tong yan wu Wen Xiao-xin Zeng Rong-jia Zhu Ya-hui Wang ling-zhi Yi Zhongye Changtian International Engineering Co. Ltd.Changsha 410205HunanChina College of Automation and Electronic Information Xiangtan UniversityXiangtan 411105HunanChina College of Electrical and Information Engineering Hunan UniversityChangsha 410082HunanChina

Proportioning is an important part of sintering,as it affects the cost of sintering and the quality of sintered *** address the problems posed by the complex raw material information and numerous constraints in the sintering process,a multi-objective optimisation model for sintering proportioning was established,which takes the proportioning cost and TFe as the optimisation ***,an improved multi-objective beluga whale optimisation(IMOBWO)algorithm was proposed to solve the nonlinear,multi-constrained multi-objective optimisation *** algorithm uses the con-strained non-dominance criterion to deal with the constraint problem in the ***,the algorithm employs an opposite learning strategy and a population guidance mechanism based on angular competition and two-population competition strategy to enhance convergence and population *** actual proportioning of a steel plant indicates that the IMOBWO algorithm applied to the ore proportioning process has good convergence and obtains the uniformly distributed Pareto ***,compared with the actual proportioning scheme,the proportioning cost is reduced by 4.3361¥/t,and the TFe content in the mixture is increased by 0.0367%in the optimal compromise ***,the proposed method effectively balances the cost and total iron,facilitating the comprehensive utilisation of sintered iron ore resources while ensuring quality assurance.

关键词： Sintering process Proportioning Iron ore Multi-objective beluga whale optimisation algorithm Proportioning cost

Gentiopicroside ameliorates LKB1/AMPK-dependent alcoholic hepatosteatosis via P2x7R-NLRP3 inflammasome

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中国药理学与毒理学杂志 2018年第4期32卷 265-266页

作者： Xia LI Yu ZHANG Kai-li XIA Min JIANG Ben-wen CUI yan-ling wu Ji-xing NAN Li-hua LIAN Key Laboratory for Natural Resource of Changbai Mountain & Functional Molecules Ministry of Education College of Pharmacy Yanbian University Yanji 133002 China

OBJECTIVE Regulating P2x7R-NLRP3 inflammasome activation might be a potential therapeutic strategy to treat alcoholic *** investigated whether this process would be modulated by gentiopicroside(GPS),which is attributed to the bitterness of gentian root *** An in vivo model was established by intragastrically treating mice with ethanol,and an in vitro model was created by treating HepG2 cells with ethanol or treating RAW 264.7 macrophages and murine bone marrow-derived macrophages(BMDMs) with lipopolysaccharides(LPS) plus adenosine ***(ATP).RESULTS In alcoholic hepatosteatotic mice model,GPS decreased serum *** and triglyceride *** regulated sterol regulatory element-binding protein-1(Srebp1),peroxisome proliferators-actived receptors α(PPARα) and acetyl CoA carboxylase(ACC) expression via elevating liver kinase B1(LKB1)/AMP-activated Kinase(AMPK).Suppression of nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3),caspase-1 and expression by GPS resulted in the inhibition of interleukin-1β(IL-1β) *** ethanol-exposed HepG2 cells,GPS reduced *** and promoted lipid oxidation via P2x7R-NLRP3 inflammasome activation.P2x7R silencing enhanced AMPK activity,and reduced Srebp1 expression in ethanol-treated *** *** P2x7R-mediated inflammatory response to extracellular ATP in LPS-primed RAW 264.7 *** and ***,P2x7R deficiency attenuated IL-1β cleavage in RAW 264.7 ***,and GPS further suppressed IL-1β *** Activation of LKB1/AMPK signaling by GPS might be mediated by P2x7R-NLRP3 inflammasome,suggesting a therapeutic utility of P2x7R blockade in alcoholic hepatosteatosis treatment.

关键词：炎症酒精肝治疗方法临床分析

Global-local combined features to detect pain intensity from facial expression images with attention mechanism

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Journal of Electronic Science and Technology 2024年第3期22卷 80-93页

作者： Jiang wu Yi Shi Shun yan Hong-Mei yan yangtze Delta Region Institute(Huzhou) University of Electronic Science and Technology of ChinaHuzhou313001China MOE Key Laboratory for Neuroinformation School of Life Science and TechnologyUniversity of Electronic Science and Technology of ChinaChengdu611731China College of Engineering University of California at Santa BarbaraSanta Barbara93106USA

The estimation of pain intensity is critical for medical diagnosis and treatment of *** the development of image monitoring technology and artificial intelligence,automatic pain assessment based on facial expression and behavioral analysis shows a potential value in clinical *** paper reports a framework of convolutional neural network with global and local attention mechanism(GLA-CNN)for the effective detection of pain intensity at four-level thresholds using facial expression ***-CNN includes two modules,namely global attention network(GANet)and local attention network(LANet).LANet is responsible for extracting representative local patch features of faces,while GANet extracts whole facial features to compensate for the ignored correlative features between *** the end,the global correlational and local subtle features are fused for the final estimation of pain *** under the UNBC-McMaster Shoulder Pain database demonstrate that GLA-CNN outperforms other state-of-the-art ***,a visualization analysis is conducted to present the feature map of GLA-CNN,intuitively showing that it can extract not only local pain features but also global correlative facial *** study demonstrates that pain assessment based on facial expression is a non-invasive and feasible method,and can be employed as an auxiliary pain assessment tool in clinical practice.

关键词： Attention Convolutional neural network Facial expression Pain intensity

Dihydroquercetin ameliorates alcoholic liver steatosis through P2x7R-NLRP3 inflammasome pathway

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中国药理学与毒理学杂志 2018年第4期32卷 338-339页

作者： Yu ZHANG Xia LI Kai-li XIA Min JIANG Ben-wen CUI yan-ling wu Ji-xing NAN Li-hua LIAN Key Laboratory for Natural Resource of Changbai Mountain & Functional Molecules Ministry of Education College of Pharmacy Yanbian University Yanji 133002 China

OBJECTIVE Dihydroquercetin(TAX) is the most abundant dihydroflavone found in ***,milk thistle and Douglas fir *** investigated whether TAX could inhibit the lipid accumulation in alcoholic liver steatosis in vivo and in *** An in vivo model was established by *** treating mice with ethanol,and an in vitro model was created by treating HepG2 cells with *** TAX regulated Sterol Regulatory Element-binding Protein-1(SREBP1) and Acetyl CoA Carboxylase(ACC) expression via elevating Liver Kinase B1(LKB1)/AMP-activated Kinase(AMPK)***,TAX upregulated SIRT1 expression,which suppressed by ethanal *** of Purinergic 2X7 receptor(P2x7R),nucleotide-binding oligomerization domain-like *** protein 3(NLRP3) and Cysteine protease-1(caspase-1) cleavage by TAX resulted in the *** of Interleukin-1β(IL-1β) production and ***,TAX reduced lipogenesis and *** lipid oxidation via the regulation of AMPK and ACC in ethanol-treated steatotic HepG2 *** downregulated IL-1β cleavage response to Lipopolysaccharides(LPS) plus adenosine triphosphate(ATP) stimulation in HepG2 cells.P2x7R deficiency attenuated lipid accumulation with increasing AMPK activity and decreasing SREBP1 expression in ethanol-treated HepG2 *** Our data showed that TAX exhibited the inhibitory properties on lipogenesis and hepatoprotective ***,indicating that TAX has therapeutic potential for preventing alcoholic liver steatosis.

关键词：二氢黄酮酒精肝治疗方法化合物

Precontrol of short-period motion for a Tension Leg Platform

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Journal of Ocean Engineering and Science 2024年第3期9卷 264-277页

作者： Hao wu yan Lin Yongxi wu School of Mechatronic Engineering and Automation Shanghai UniversityShanghai 200444China Department of Naval Architecture Dalian University of TechnologyDalian 116023China State Key Laboratory of Structural Analysis for Industrial Equipment Dalian University of TechnologyDalian 116023China School of Mechanics and Engineering Science Shanghai UniversityShanghai 200444China

The Tension Leg Platform(TLP)is a hybrid,compliant platform designed to sustain springing and ringing responses that are correlated to short-period *** the period of short-period motion is within the wave energy concentration region,TLPs may experience sensitive short-period motion,such as resonance and green water,that usually cause serious damage to *** this study,a precontrol methodology is presented as a solution to prevent TLP-sensitive short-period *** applying the precontrol methodology,the parameters of TLP can be predetermined,allowing TLP motion performance to meet the requirements of short-period motion before sensitive motions actually *** example,the damping coefficient should be less than 4.3,the tendons’stiffness should be larger than 0.91×10^(8),and the dimensionless draft should be less than *** development of a precontrol methodology is based on a solid theoretical ***,a series of simple and high-fidelity numerical models are proposed to simulate the natural period of roll,natural period of heave,and green water ***,a constraint regime is generated based on the numerical models and the sensitive motion range of short-period *** constraint regime is divided into two parts:the control range(corresponding to sensitive shortperiod motion)and the feasible range(the complementary set of control ranges in the whole parameter constraint domain).Finally,TLP parameters are derived from the calculated feasible *** precontrol methodology goes beyond the conventional approach of real-time control by changing the control from a remedial action to a preventive action.

关键词： Precontrol Constraint regime Short-period motion TLP

Selection Method for New Energy Output Guaranteed Rates Considering Optimal Energy Storage Configuration

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CSEE Journal of Power and Energy Systems 2024年第2期10卷 539-547页

作者： Xin Cheng Tong wu Wei Yao yan yang Guangdong Power Grid Corporation Guangzhou 510620China State Key Laboratory of Advanced Electromagnetic Engineering and Technology and School of Electrical and Electronic EngineeringHuazhong University of Science and TechnologyWuhan 430074China

The output of new energy represented by wind power and photovoltaic power features volatility and randomness. It is a practical approach to use the guaranteed rate with statistical characteristics to analyze the output coefficient of new energy. However, there is a lack of analysis and demonstration on the value of the new energy output guaranteed rate. To solve this problem, a selection method for the new energy output guaranteed rate considering optimal energy storage configuration is proposed. First, the influence of the new energy output guaranteed rate on the new energy output coefficient is analyzed. Secondly, with the goal of minimizing the comprehensive costs, an optimal configuration model of energy storage based on the output guaranteed rate of new energy is constructed, and particle swarm optimization is used for the optimization solution. Then, according to the optimal energy storage configuration results under different guaranteed rates, a reasonable new energy output guaranteed rate is determined by maximizing the comprehensive benefits obtained from energy storage. Finally, an example applied to the power grid in an area of Guangdong in Southern China is provided to illustrate the effectiveness of the proposed method.

关键词： Energystorage optimization guaranteed output guaranteed rate new energy consumption

Resource allocation for D2D-assisted haptic communications

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Digital Communications and Networks 2024年第1期10卷 63-74页

作者： yan wu Chao Yue yang yang Liang Ao College of Telecommunication Engineering Army Engineering University of PLANanjing210000China

Haptic communications is recognized as a promising enabler of extensive services by enabling real-time haptic control and feedback in remote environments,e.g.,teleoperation and autonomous *** the strict transmission requirements on reliability and latency,Device-to-Device(D2D)communications is introduced to assist haptic *** particular,the teleoperators with poor channel quality are assisted by auxiliaries,and each auxiliary and its corresponding teleoperator constitute a D2D ***,the haptic interaction and the scarcity of radio resources pose severe challenges to the resource allocation,especially facing the sporadic packet ***,the contentionbased access scheme is applied to achieve low-latency transmission,where the resource scheduling latency is omitted and users can directly access available *** this context,we derive the reliability index of D2D pairs under the contention-based access scheme,i.e.,closed-loop packet error ***,the reliability performance is guaranteed by bidirectional power control,which aims to minimize the sum packet error probability of all D2D *** game theory is introduced to solve the problem with low ***,a distributed power control algorithm based on synchronous log-linear learning is proposed to converge to the optimal Nash *** results demonstrate the superiority of the proposed learning algorithm.

关键词： Haptic communications D2D Power control Contention-based access Potential game

Mixing effects of high-speed jets in gas-solid riser and downer reactors

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Particuology 2024年第9期92卷 196-209页

作者： Zihan yan Dongdong Wang Lining wu Chunxi Lu College of Chemical Engineering and Environment State Key Laboratory of Heavy Oil ProcessingChina University of Petroleum-BeijingBeijing102249China

For the high-temperature and short-contact time gas-solid reaction process,riser and downer are considered appropriate *** realize an intensive and complete mixing of reactants with catalysts,the feed raw is always introduced in the form of high-speed *** this study,in order to investigate the mixing effects of different types of high-speed jets in riser and downer,traceable ozone is injected with the high-speed feed jets to react with catalyst *** detecting the decomposition of ozone,the gas-solid mixing and reaction in riser and downer under the influence of both co-current and counter-current injections are *** relative ozone concentration is used to reflect the location reaction extent and its radial nonuniformity index is proposed to compare the results in riser and *** is found that the jet influence zone in downer provides a relatively better environment for the mixing of feed jets with *** the riser,introduction of counter-current injections could improve the uniformity of gas-solid mixing in the initial contact region of feed with catalysts.

关键词： Riser Downer Gas-solid two-phase flow Jet