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Effect of vitrification at the germinal vesicle stage on the global methylation status in mouse oocytes subsequently matured in vitro

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Chinese Medical Journal 2014年第23期127卷 4019-4024页

作者： yan jie Zhang lu Wang Tianren Li Rong Liu Ping yan Liying Qiao jie Department of Obstetrics and Gynecology Peking University Third Hospital Key Laboratory of Assisted Reproduction Ministry of Education Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Beijing 100191 China

Background It is still unclear whether the vitrification procedure itself is associated with the incidence of abnormal DNA methylation during oocytes *** purpose of this study was to evaluate the epigenetic profile of mouse oocytes,which went through vitrification either at a mature stage or at an immature stage following in vitro maturation （IVM） by analyzing the global DNA *** Metaphase Ⅱ （M Ⅱ） stage and germinal vesicle （GV） stage oocytes were collected from adult female mice and were vitrified *** M Ⅱ oocytes were assessed for cryo-survival and global DNA *** GV oocytes were assessed for cryo-survival and only the surviving GV oocytes were cultured in vitro for subsequent assessment of global DNA methylation in mature *** vivo matured fresh M Ⅱ oocytes without undergoing vitrification were used as *** level of global DNA methylation in the M Ⅱ oocytes was then examined by immunofluorescence using an anti-5-methylcytosine （anti-5-MeC） monoclonal antibody and fluorescein isothiocyanate （FITC）-conjugated goat anti-mouse IgG under a laser scanning confocal *** In terms of the effect of vitrification on global DNA methylation status in matured oocytes,in the M Ⅱ-v group,all the examined oocytes （90/90） were found with hypermethylation,including 63.3％（57/90） of them displaying DNA methylation of a very high level,25.6％（23/90） with a high level,and 11.1％（10/90） with an intermediate level,whereas in the GV-v group,all the matured oocytes （129/129） were also examined with hypermethylation,including 67.4％（87/129） of them displaying DNA methylation of a very high level,23.3％（30/129） with a high level,and 9.3％（12/129） with an intermediate ***,it was similar between both groups,which were similar to the control：68.6％（83/121） of fresh M Ⅱ oocytes displayed DNA methylation of a very high level,21.5％（26/121） with a high level,and 9.9％（12/121） with an intermediate level （

关键词： DNA methylation in vitro maturation oocyte vitrification epigenetic safety

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Role of cathepsin B-mediated apoptosis in fulminant hepatic failure in mice

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World Journal of Gastroenterology 2009年第10期15卷 1231-1236页

作者： Bing-Zhu yan Wei Wang Li-yan Chen Man-Ru Bi yan-jie lu Bao-Xin Li Bao-Shan yang Department of Infectious Diseases the SecondClinical Hospital of Harbin Medical University Harbin 150086China Department of Pharmacology HarbinMedical University Harbin 150086 China

AIM: To investigate the pathogenic role of cathepsin B and the protective effect of a cathepsin B inhibitor (CA074Me) in fulminant hepatic failure in mice. METHODS: LPS/D-Gal N was injected into mice of the model group to induce fulminant hepatic failure; the protected group was administered CA-074me for 30 min before LPS/D-Gal N treatment; the normal group was given isochoric physiologic saline. Liver tissue histopathology was determined with HE at 2, 4, 6 and 8 h after Lps/D-Gal injection. Hepatocyte apoptosis was examined by TUNEL method. The expression of cathepsin B in liver tissues was investigated by immunohistochemistry, Western blot and RT-PCR. RESULTS: Compared with the normal group, massive typical hepatocyte apoptosis occurred in the model group; the number of apoptotic cells reached a maximum 6 h after injection. The apoptosis index (AI) in the protected group was clearly reduced (30.4 ± 2.8 vs 18.1 ± 2.0, P luSION: Cathepsin B plays an essential role in the pathogenesis of fulminant hepatic failure, and the cathepsin B inhibitor CA-074me can attenuate apoptosis and liver injury.

关键词： Fulminant hepatic failure Hepatocyteapoptosis Cathepsin B CA-O74me

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Ru doping triggering reconstruction of cobalt phosphide for coupling glycerol electrooxidation with seawater electrolysis

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Journal of Energy Chemistry 2025年第1期100卷 317-326页

作者： Binglu Deng jie Shen Jinxing lu Chuqiang Huang Zhuoyuan Chen Feng Peng Yunpeng Liu School of Materials Science and Hydrogen Energy Foshan University School of Chemistry and Chemical Engineering Guangzhou University Guangdong Key Laboratory for Hydrogen Energy Technologies

Seawater electrolysis is a promising approach for sustainable energy without relying on precious ***,the large-scale seawater electrolysis is hindered by low catalytic efficiency and severe anode corrosion caused by the harmful *** contrast to the oxygen evolution reaction （OER）and chlorin ion oxidation reaction （ClOR）,glycerol oxidation reaction （GOR） is more thermodynamically and kinetically favorable ***,a Ru doping cobalt phosphide （Ru-CoP2） is proposed as a robust bifunctional electrocatalyst for seawater electrolysis and GOR,for the concurrent productions of hydrogen and value-added *** in situ and ex situ characterization analyses demonstrated that Ru doping featured in the dynamic reconstruction process from Ru-CoP2to Ru-CoOOH,accounting for the superior GOR *** coupling GOR with hydrogen evolution was realized by employing Ru-CoP2as both anode and cathode,requiring only a low voltage of 1.43 V at 100 mA cm-2,which was 250 m V lower than that in alkaline *** work guides the design of bifunctional electrocatalysts for energy-efficient seawater electrolysis coupled with biomass resource upcycling.

关键词： Glycerol electrooxidation Hydrogen evolution Ru doping Cobalt phosphide Bifunctional electrocatalysts

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国庆70周年甘肃彩车车辆改装设计

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移动电源与车辆 2019年第4期50卷 1-5页

作者：颜鲁杰兰州电源车辆研究所有限公司兰州730050 甘肃省内燃机电站工程技术研究中心兰州730050

简要介绍国庆70周年甘肃彩车车辆改装设计的整体设计思路、具体布局及效果展示.

关键词：结构设计桁架承载结构供电系统

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H∞ Information Fusion Filtering for Discrete-time Systems with Time-delay Sensors

H∞ Information Fusion Filtering for Discrete-time Systems w...

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2009中国控制与决策会议

作者： jie yan,Xiao lu Key Laboratory for Robot & Intelligent Technology of Shandong Province Shandong University of Science and Technology Qingdao 266510,P.R.China

This paper is concerned with Hinformation fusion filtering for discrete-time systems with time-delay sensors based on re-organization of *** key technique applied for deriving the filter is the ro-organized innovation analysis approach in Krein *** Hfilter is given in terms of solutions of Riccati matrix differential *** is the first time to present the result of Hinformation fusion for linear discrete-time systems with delayed *** the fusion center,It only requires computing scalar weights,so the calculation burden can be reduced,while this method does not requires that the parameters of the system and the statistics of the noises are assumed to be known *** the proposed approach is much more computational attractive.

关键词： Information fusion H∞ filter Delayed sensors Innovation analysis Riccati equations Discrete-time systems

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Storage time affects the level and diagnostic efficacy of plasma biomarkers for neurodegenerative diseases

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Neural Regeneration Research 2025年第8期20卷 2373-2381页

作者： Lifang Zhao Mingkai Zhang Qimeng Li Xuemin Wang jie lu Ying Han yanning Cai Department of Clinical Biobank Xuanwu HospitalCapital Medical UniversityBeijingChina Beijing Geriatric Medical Research Center BeijingChina Department of Neurology Xuanwu HospitalCapital Medical UniversityBeijingChina Department of Radiology and Nuclear Medicine Xuanwu HospitalCapital Medical UniversityBeijingChina Beijing Key Laboratory of Magnetic Resonance Imaging and Brain Informatics BeijingChina Key Laboratory of Neurodegenerative diseases Ministry of EducationBeijingChina School of Biomedical Engineering Hainan UniversityHaikouHainan ProvinceChina Center of Alzheimer’s Disease Beijing Institute for Brain DisordersBeijingChina National Clinical Research Center for Geriatric Diseases BeijingChina Institute of Biomedical Engineering Shenzhen Bay LaboratoryGaoke Innovation CenterShenzhenGuangdong ProvinceChina Department of Neurobiology Xuanwu HospitalCapital Medical UniversityBeijingChina

Several promising plasma biomarker proteins,such as amyloid-β(Aβ),tau,neurofilament light chain,and glial fibrillary acidic protein,are widely used for the diagnosis of neurodegenerative ***,little is known about the long-term stability of these biomarker proteins in plasma samples stored at-80°*** aimed to explore how storage time would affect the diagnostic accuracy of these biomarkers using a large *** samples from 229 cognitively unimpaired individuals,encompassing healthy controls and those experiencing subjective cognitive decline,as well as 99 patients with cognitive impairment,comprising those with mild cognitive impairment and dementia,were acquired from the Sino Longitudinal Study on Cognitive Decline *** samples were stored at-80°C for up to 6 years before being used in this *** results showed that plasma levels of Aβ42,Aβ40,neurofilament light chain,and glial fibrillary acidic protein were not significantly correlated with sample storage ***,the level of total tau showed a negative correlation with sample storage ***,in individuals without cognitive impairment,plasma levels of total protein and tau phosphorylated protein threonine 181(p-tau181)also showed a negative correlation with sample storage *** was not observed in individuals with cognitive ***,we speculate that the diagnostic accuracy of plasma p-tau181 and the p-tau181 to total tau ratio may be influenced by sample storage ***,caution is advised when using these plasma biomarkers for the identification of neurodegenerative diseases,such as Alzheimer's ***,in cohort studies,it is important to consider the impact of storage time on the overall results.

关键词： Alzheimer’s disease amyloid-β diagnostic ability glial fibrillary acidic protein neurodegeneration neurofilament light chain plasma biomarkers single molecule array storage time tau

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未成年人罪错行为的处遇困境及应对出路——以我国近5年90件未成年人涉罪新闻报道为研究样本

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四川警察学院学报 2019年第4期31卷 75-83页

作者：张垚严露婕杭州经济技术开发区人民检察院浙江杭州310018 浙江财经大学浙江杭州310018

近年来,犯罪低龄化发展趋势明显。以刑事责任年龄为突破口的治理路径遭遇“降低”与“维持”两种观点争议。与其过分关注立法治理,倒不如将治理思维转向对罪错行为的研究。未成年人罪错行为具有罪错事件的低龄化、罪错行为的危害性、不... 详细信息

近年来,犯罪低龄化发展趋势明显。以刑事责任年龄为突破口的治理路径遭遇“降低”与“维持”两种观点争议。与其过分关注立法治理,倒不如将治理思维转向对罪错行为的研究。未成年人罪错行为具有罪错事件的低龄化、罪错行为的危害性、不良行为的渗透性三大特征。其原因在于家庭、学校、社会、行为处遇四个方面。基于此,应当通过构建罪错行为分级处遇制度,落实未成年人保护主体责任,倡导国家监护支持综合体系予以应对。

关键词：罪错行为特征产生原因治理路径

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Nanocarrier-mediated siRNA delivery:a new approach for the treatment of traumatic brain injury-related Alzheimer's disease

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Neural Regeneration Research 2025年第9期20卷 2538-2555页

作者： jie Jin Huajing Zhang Qianying lu Linqiang Tian Sanqiao Yao Feng Lai yangfan Liang Chuanchuan Liu Yujia lu Sijia Tian yanmei Zhao Wenjie Ren Institute of Disaster and Emergency Medicine Tianjin UniversityTianjinChina Key Laboratory for Disaster Medicine Technology TianjinChina Henan Medical Key Laboratory for Research of Trauma and Orthopedics The Third Affiliated Hospital of Xinxiang Medical UniversityXinxiangHenan ProvinceChina Clinical Medical Center of Tissue Engineering and Regeneration Xinxiang Medical UniversityXinxiangHenan ProvinceChina Institutes of Health Central Plain Xinxiang Medical UniversityXinxiangHenan ProvinceChina School of Public Health Xinxiang Medical UniversityXinxiangHenan ProvinceChina

Traumatic brain injury and Alzheimer's disease share pathological similarities,including neuronal loss,amyloid-βdeposition,tau hyperphosphorylation,blood-brain barrier dysfunction,neuroinflammation,and cognitive ***,traumatic brain injury can exacerbate Alzheimer's disease-like pathologies,potentially leading to the development of Alzheimer's *** offer a potential solution by facilitating the delive ry of small interfering RNAs across the blood-brain barrier for the targeted silencing of key pathological genes implicated in traumatic brain injury and Alzheimer's disease.U nlike traditional approaches to neuro regeneration,this is a molecula r-targeted strategy,thus avoiding non-specific drug *** review focuses on the use of nanocarrier systems for the efficient and precise delive ry of siRNAs,discussing the advantages,challenges,and future *** principle,siRNAs have the potential to target all genes and non-targetable protein s,holding significant promise for treating various *** the various therapeutic approaches currently available for neurological diseases,siRNA gene silencing can precisely"turn off"the expression of any gene at the genetic level,thus radically inhibiting disease progression;however,a significant challenge lies in delivering siRNAs across the blood-brain *** have received increasing attention as an innovative drug delive ry tool fo r the treatment of brain *** are considered a potential therapeutic strategy with the advantages of being able to cross the blood-brain barrier,targeted drug delivery,enhanced drug stability,and multifunctional *** use of nanoparticles to deliver specific modified siRNAs to the injured brain is gradually being recognized as a feasible and effective *** this strategy is still in the preclinical exploration stage,it is expected to achieve clinical translation in the future,creating a new field of molecular tar

关键词： blood-brain barrier cognitive dysfunction exosomes gene silencing immunotherapy inorganic nanomaterials liposome nanoparticles neuroinflammation polymer nanoparticles stem cells

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草原生态系统植物地下生物量分配及对全球变化的响应

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植物生态学报 2017年第5期41卷 585-596页

作者：严月朱建军张彬张艳杰鲁顺保潘庆民江西师范大学鄱阳湖湿地与流域研究教育部重点实验室/江西省亚热带植物资源保护与利用重点实验室/江西师范大学生命科学学院南昌330022 中国科学院内蒙古草业研究中心北京100093 中国科学院植物研究所植被与环境变化国家重点实验室北京100093 内蒙古农业大学生态环境学院呼和浩特010018

草原生态系统植物生物量在地上和地下器官之间的分配是联系地上生产力与地下碳固持的枢轴环节。由于地下生物量的准确测定是正确分析生物量分配的基础,该文首先系统分析了植物地下生物量的测定方法及其优缺点;在此基础上,综述了当前草... 详细信息

草原生态系统植物生物量在地上和地下器官之间的分配是联系地上生产力与地下碳固持的枢轴环节。由于地下生物量的准确测定是正确分析生物量分配的基础,该文首先系统分析了植物地下生物量的测定方法及其优缺点;在此基础上,综述了当前草地生态系统地下生物量分配对全球气候变化主要驱动因子(降水、CO_2浓度、温度和氮沉降)的响应,并提出了今后相关研究中需解决的主要问题,以期为开展全球变化背景下草地生态系统碳循环的研究提供参考。

关键词：根冠比地下生物量降水格局大气温度 CO2浓度氮沉降

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High Fe‑Loading Single‑Atom Catalyst Boosts ROS Production by Density Effect for Efficient Antibacterial Therapy

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Nano-Micro Letters 2025年第2期17卷 187-203页

作者： Si Chen Fang Huang Lijie Mao Zhimin Zhang Han Lin Qixin yan Xiangyu lu Jianlin Shi Department of Cardiology Shanghai Tenth People’s HospitalSchool of MedicineTongji UniversityShanghai 200092People’s Republic of China Shanghai Institute of Ceramics Chinese Academy of SciencesShanghai 200050People’s Republic of China Shanghai Frontiers Science Center of Nanocatalytic Medicine School of MedicineTongji UniversityShanghai 200092People’s Republic of China

The current single-atom catalysts(SACs)for medicine still suffer from the limited active site ***,we develop a synthetic method capable of increasing both the metal loading and mass-specific activity of SACs by exchanging zinc with *** constructed iron SACs(h^(3)-FNC)with a high metal loading of 6.27 wt%and an optimized adjacent Fe distance of~4 A exhibit excellent oxidase-like catalytic performance without significant activity decay after being stored for six months and promising antibacterial ***,a“density effect”has been found at a high-enough metal doping amount,at which individual active sites become close enough to interact with each other and alter the electronic structure,resulting in significantly boosted intrinsic activity of single-atomic iron sites in h^(3)-FNCs by 2.3 times compared to low-and medium-loading ***,the overall catalytic activity of h^(3)-FNC is highly improved,with mass activity and metal mass-specific activity that are,respectively,66 and 315 times higher than those of commercial Pt/*** addition,h^(3)-FNCs demonstrate efficiently enhanced capability in catalyzing oxygen reduction into superoxide anion(O_(2)·^(−))and glutathione(GSH)*** in vitro and in vivo assays demonstrate the superior antibacterial efficacy of h^(3)-FNCs in promoting wound *** work presents an intriguing activity-enhancement effect in catalysts and exhibits impressive therapeutic efficacy in combating bacterial infections.

关键词： Nanocatalytic medicine Single-atom catalysts Reactive oxygen species(ROS) High metal loading Oxidase catalysis

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