检索结果-南通市图书馆

Multiplex gene editing reduces oxalate production in primary hyperoxaluria type 1

Zoological Research 2023年第6期44卷 993-1002页

作者： Rui Zheng De-Xin Zhang yan-jiao shao Xiao-Liang Fang Lei yang Ya-Nan Huo Da-Li Li Hong-Quan Geng Department of Pediatric Urology Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China Shanghai Key Laboratory of Regulatory Biology Institute of Biomedical Sciences and School of Life SciencesEast China Normal UniversityShanghai 200241China Division of Pediatric Urology Children’s Hospital of Fudan UniversityShanghai 201102China

Targeting key enzymes that generate oxalate precursors or substrates is an alternative strategy to eliminate primary hyperoxaluria type I(PH1),the most common and lifethreatening type of primary *** compact Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)from the Prevotella and Francisella 1(Cpf1)protein simplifies multiplex gene editing and allows for all-in-one adeno-associated virus(AAV)*** hypothesized that the multiplex capabilities of the Cpf1system could help minimize oxalate formation in PH1 by simultaneously targeting the hepatic hydroxyacid oxidase 1(Hao1)and lactate dehydrogenase A(Ldha)*** cohorts included treated PH1 rats(Agxt Q84X rats injected with AAV-AsCpf1 at 7 days of age),phosphate-buffered saline(PBS)-injected PH1 rats,untreated PH1 rats,and age-matched wild-type(WT)*** most efficient and specific CRISPR RNA(crRNA)pairs targeting the rat Hao1and Ldha genes were initially screened ex *** vivo experiments demonstrated efficient genome editing of the Hao1 and Ldha genes,primarily resulting in small *** resulted in decreased transcription and translational expression of Hao1 and *** significantly reduced urine oxalate levels,reduced kidney damage,and alleviated nephrocalcinosis in rats with *** liver toxicity,ex-liver genome editing,or obvious offtarget effects were *** demonstrated the AAVAsCpf1 system can target multiple genes and rescue the pathogenic phenotype in PH1,serving as a proof-ofconcept for the development of multiplex genome editingbased gene therapy.

关键词： Hyperoxaluria Genome editing Lactate dehydrogenase Hydroxyacid oxidase 1

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

即食复合辣木果蔬薄片加工工艺的研究

引用

保鲜与加工 2017年第5期17卷 81-87,95页

作者：龙娇妍李少华河南牧业经济学院河南郑州450011 河南职业技术学院烹饪食品系河南郑州450046

以辣木、猕猴桃和紫菜为主要原料,对辣木果蔬薄片生产中的几个关键工艺进行了研究,包括烫漂方法、调味料的配比、增稠剂的选择、干燥方法等4个最佳工艺参数的确定。结果表明,用90℃热水对复合蔬菜热烫30 s时,其VC、黄酮含量最高,分别为0... 详细信息

以辣木、猕猴桃和紫菜为主要原料,对辣木果蔬薄片生产中的几个关键工艺进行了研究,包括烫漂方法、调味料的配比、增稠剂的选择、干燥方法等4个最佳工艺参数的确定。结果表明,用90℃热水对复合蔬菜热烫30 s时,其VC、黄酮含量最高,分别为0.123 g/100 g、2.40%;复合辣木果蔬泥的最佳调味配方为:以浓缩后复合果蔬质量计100%,食盐添加量1.1%、味精添加量0.9%、白砂糖添加量1.5%,五香粉添加量0.9%,该配方下制得的复合辣木果蔬泥调味感官品质评分为14.5分;复合增稠剂的最佳配方为:CMC-Na添加量0.18%,黄原胶添加量0.19%,海藻酸钠添加量0.18%,在此条件下复合辣木果蔬泥的感官品质评分为96.1分。采用80℃热风干燥1.5 h,再用中档火力的微波炉干燥3 min进行联合干燥,此时得到的复合辣木果蔬薄片的VC含量损失较小,含水率适中,产品酥脆可口,感官品质评分为84.1分。

关键词：辣木果蔬薄片感官评价烫漂正交试验

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

High-energy-density lithium manganese iron phosphate for lithium-ion batteries: Progresses, challenges, and prospects

引用

Journal of Energy Chemistry 2025年第1期100卷 1-17页

作者： Bokun Zhang Xiaoyun Wang Shuai Wang yan Li Libo Chen Handong jiao Zhijing Yu Jiguo Tu State Key Laboratory of Advanced Metallurgy University of Science and Technology Beijing Research Institute for Intelligent Wearable Systems The Hong Kong Polytechnic University Institute of Advanced Structure Technology Beijing Institute of Technology

The soaring demand for smart portable electronics and electric vehicles is propelling the advancements in high-energy–density lithium-ion *** manganese iron phosphate (LiMnxFe1-xPO4) has garnered significant attention as a promising positive electrode material for lithium-ion batteries due to its advantages of low cost,high safety,long cycle life,high voltage,good high-temperature performance,and high energy *** LiMnxFe1-xPO4has made significant breakthroughs in the past few decades,there are still facing great challenges in poor electronic conductivity and Li-ion diffusion,manganese dissolution affecting battery cycling performance,as well as low tap *** review systematically summarizes the reaction mechanisms,various synthesis methods,and electrochemical properties of LiMnxFe1-xPO4to analyze reaction processes accurately and guide material ***,the main challenges currently faced are concluded,and the corresponding various modification strategies are discussed to enhance the reaction kinetics and electrochemical performance of LiMnxFe1-xPO4,including multi-scale particle regulation,heteroatom doping,surface coating,as well as microscopic morphology ***,in view of the current research challenges faced by intrinsic reaction processes,kinetics,and energy storage applications,the promising research directions are *** importantly,it is expected to provide key insights into the development of high-performance and stable LiMnxFe1-xPO4materials,to achieve practical energy storage requirements.

关键词： Lithium manganese iron phosphate High energy density Lithium-ion batteries Reaction mechanism Tap density

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Pathogenesis, diagnosis, and treatment of epilepsy: electromagnetic stimulation-mediated neuromodulation therapy and new technologies

引用

Neural Regeneration Research 2025年第4期20卷 917-935页

作者： Dian jiao Lai Xu Zhen Gu Hua yan Dingding Shen Xiaosong Gu Academy of Medical Engineering and Translational Medicine Tianjin UniversityTianjinChina Key Laboratory of Advanced Drug Delivery Systems of Zhejiang Province College of Pharmaceutical SciencesZhejiang UniversityHangzhouZhejiang ProvinceChina Affiliated Hospital of Nantong University NantongJiangsu ProvinceChina

Epilepsy is a severe,relapsing,and multifactorial neurological *** regarding the accurate diagnosis,prognosis,and in-depth pathogenesis are crucial for the precise and effective treatment of *** pathogenesis of epilepsy is complex and involves alterations in variables such as gene expression,protein expression,ion channel activity,energy metabolites,and gut microbiota *** results are lacking for conventional treatments for *** resection of lesions,drug therapy,and non-drug interventions are mainly used in clinical practice to treat pain associated with ***-pharmacological treatments,such as a ketogenic diet,gene therapy for nerve regeneration,and neural regulation,are currently areas of research *** review provides a comprehensive overview of the pathogenesis,diagnostic methods,and treatments of *** also elaborates on the theoretical basis,treatment modes,and effects of invasive nerve stimulation in neurotherapy,including percutaneous vagus nerve stimulation,deep brain electrical stimulation,repetitive nerve electrical stimulation,in addition to non-invasive transcranial magnetic stimulation and transcranial direct current *** studies have shown that electromagnetic stimulation-mediated neuromodulation therapy can markedly improve neurological function and reduce the frequency of epileptic ***,many new technologies for the diagnosis and treatment of epilepsy are being ***,current research is mainly focused on analyzing patients’clinical manifestations and exploring relevant diagnostic and treatment methods to study the pathogenesis at a molecular level,which has led to a lack of consensus regarding the mechanisms related to the disease.

关键词： diagnosis drug treatment electroencephalography epilepsy monitoring epilepsy nerve regeneration neurostimulation non-drug interventions pathogenesis prediction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Imperative for long-term management and surveillance in Kawasaki disease

引用

World Journal of Clinical Cases 2025年第4期13卷 61-63页

作者： yan Pan Fu-Yong jiao Department of Pediatrics The First Affiliated Hospital of Yangtze UniversityJingzhou 434000Hubei ProvinceChina Shaanxi Kawasaki Disease Diagnosis and Treatment Center Children’s HospitalShaanxi Provincial People’s Hospital of Xi’an Jiaotong UniversityXi’an 710000Shaanxi ProvinceChina

Kawasaki disease(KD)is a significant pediatric vasculitis known for its potential to cause severe coronary artery *** the effectiveness of initial treatments,such as intravenous immunoglobulin,KD patients can experience long-term cardiovascular issues,as evidenced by a recent case report of an adult who suffered a ST-segment elevation myocardial infarction due to previous KD in the World Journal of Clinical *** editorial emphasizes the critical need for long-term management and regular surveillance to prevent such *** drawing on recent research and case studies,we advocate for a structured approach to follow-up care that includes routine cardiac evaluations and preventive measures.

关键词： Kawasaki disease Long-term management Coronary artery aneurysm Surveillance Preventive care

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asymptotic false discovery control of the Benjamini-Hochberg procedure for pairwise comparisons

引用

Science China Mathematics 2025年第1期 223-252页

作者： Weidong Liu Dennis Leung Qi-Man shao School of Mathematical Sciences Shanghai Jiao Tong University School of Mathematics and Statistics University of Melbourne Department of Statistics and Data Science SICMSouthern University of Science and Technology

In a one-way analysis-of-variance(ANOVA) model,the number of pairwise comparisons can become large even with a moderate number of *** by this,we consider a regime with a growing number of groups and prove that,when testing pairwise comparisons,the Benjamini-Hochberg(BH) procedure can asymptotically control false discoveries,despite the fact that the involved t-statistics do not exhibit the wellknown positive dependence structure required for exact false discovery rate(FDR) *** Tukey's perspective that the difference between the means of any two groups cannot be exactly zero,our main result provides control over the directional false discovery rate and directional false discovery proportion.A key technical contribution of our work is demonstrating that the dependence among the t-statistics is sufficiently weak to establish the convergence result typically required for asymptotic FDR *** analysis does not rely on conventional assumptions such as normality,variance homogeneity,or a balanced design,thereby offering a theoretical foundation for applications in more general settings.

关键词：

来源：

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Adaptive Coordinated Control for Hot Strip Finishing Mills

引用

Journal of Iron and Steel Research International 2011年第4期18卷 36-43页

作者： jiao Xiao-hong shao Li-ping PENG yan Institute of Electrical Engineering Yanshan University Qinhuangdao 066004 Hebei China Institute of Mechanical Engineering Yanshan University Qinhuangclao 066004 Hebei China

To further improve the control accuracy for strip dimension of hot strip mills,an adaptive control scheme is investigated for a hot strip finishing mill based on the decentralization and coordination among the controllers of gauge,tension and ***,the adaptive controller designed can regulate simultaneously the strip exit thickness,the strip tension and the looper angle to ensure better performance of the strip quality of finishing ***,the control scheme is proposed in consideration of the essential nonlinearity and the unavoidable friction phenomena in the mechanical system,so the controller can be efficient in a wider range of working *** simulation results of a model obtained from a real hot strip finishing mill show the effectiveness of the proposed control scheme in comparison with the conventional control method.

关键词： hot strip finishing mills exist gauge tension looper angle adaptive control

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Systematic study of H2 production from catalytic photoreforming of cellulose over Pt catalysts supported on TiO2

引用

Chinese Journal of Chemical Engineering 2020年第8期28卷 2084-2091页

作者： Lan Lan yan shao Yilai jiao Rongxin Zhang Christopher Hardacre Xiaolei Fan Department of Chemical Engineering and Analytical Science School of EngineeringThe University of ManchesterM139PLUnited Kingdom School of Chemical and Environmental Engineering Wuyi UniversityJiangmen 52920China Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China

Hydrogen(H2)production from photocatalytic reforming of cellulose is a promising way for sustainable H2 to be ***,we report a systematic study of the photocatalytic reforming of cellulose over Pt/m-TiO2(*** TiO2,80%of anatase and 20%of rutile)catalysts in *** optimum operation condition was established by studying the effect of Pt loading,catalyst concentration,cellulose concentration and reaction temperature on the gas production rate of H2(r(H2))and CO2(r(CO2)),suggesting an optimum operation condition at 40°C with 1.0 g·L^-1of cellulose and 0.75 g·L^-1of 0.16-Pt/m-TiO2 catalyst(with 0.16 wt%Pt loadting)to achieve a relatively sound photocatalytic performance with rH2=9.95μmol·h^-*** is also shown that although the photoreforming of cellulose was operated at a relatively mild condition(*** an UV-A lamp irradiation at40°C in the aqueous system),a low loading of Pt at^0.16 wt%on m-TiO2 could promote the H2 production ***,by comparing the reaction order expressed from both r(H2)(a1)and r(CO2)(a2)with respect to cellulose and water,the possible mechanism of H2 production was proposed.

关键词： Hydrogen(H2)production Catalytic photoreforming Cellulose Kinetics Titanium dioxide(TiO2)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Analysis of the 3C 445 soft X-ray spectrum as observed by Chandra high-energy gratings

引用

Research in Astronomy and Astrophysics 2018年第5期18卷 59-68页

作者： Fu-Tong Dong Shu-Hua shao yan Cheng jiao-Long Zeng Technological Vocational College of Dezhou Dezhou 251200 China College of Science National University of Defense Technology Changsha 410073 China

We present a detailed analysis of the soft X-ray emission of 3 C 445 using an archival Chandra High Energy Transmission Grating（HETG） spectrum. Highly-ionized H-and He-like Mg, Si and S lines, as well as a resolved low-ionized Si Kα line, are detected in the high resolution spectrum. The He-like triplets of Mg and Si are resolved into individual lines, and the calculated R ratios indicate a high density for the emitter. The low values of G ratios indicate the lines originate from collisionally ionized plasmas. However, the detection of a resolved narrow Ne X radiative recombination continua（RRC） feature in the spectrum seems to prefer a photoionized environment. The spectrum is subsequently modeled with a photoionization model, and the results are compared with those of a collisional model. Through a detailed analysis of the spectrum, we exclude a collisional origin for these emission lines. A one-component photoionization model provides a great fit to the emission features. The best-fit parameters are log ξ = 3.3^＋0.4-0.3 erg cm s^-1, n H = 5^＋15 -4.5× 10^10 cm^-3 and NH = 2.5^＋3.8 -1.7× 10^20 cm^-*** to the calculated high density for the emitter, the measured velocity widths of the emission lines and the inferred radial distance（6 × 10^14-8 × 10^15 cm）, we suggest the emission lines originating from matter are located in the broad line region（BLR）.

关键词： galaxies active - X-rays galaxies - galaxies individual (3C 445)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Solute transport characteristics of a deep soil profile in the Loess Plateau,China

引用

Journal of Arid Land 2018年第4期10卷 628-637页

作者： WANG jiao shao Ming'an Key Laboratory of Ecosystems Network Observation and Modeling Institute of Geographical Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau Northwest A&F UniversityYangling 712100China

Understanding solute transport behaviors of deep soil profile in the Loess Plateau is helpful for ecological construction and agricultural production improvement. In this study, solute transport processes of a deep soil profile were measured by a conservative tracer experiment using 25 undisturbed soil cores （20 cm long and 7 cm diameter for each） continuously sampled from the surface downward to the depth of 500 cm in the Loess Plateau of China. The solute transport breakthrough curves （BTCs） were analyzed in terms of the convection-dispersion equation （CDE） and the mobile-immobile model （MIM）. Average pore-water velocity and dispersion coefficient （or effective dispersion coefficient） were calculated using the CDE and MIM. Basic soil properties and water infiltration parameters were also determined to explore their influence on the solute transport parameters. Both pore-water velocity and dispersion coefficient （or effective dispersion coefficient） generally decreased with increasing depth, and the dispersivity fluctuated along the soil profile. There was a good linear correlation between log-transformed pore-water velocity and dispersion coefficient, with a slope of about 1.0 and an average dispersivity of 0.25 for the entire soil profile. Generally speaking, the soil was more homogeneous along the soil profile. Our results also show that hydrodynamic dispersion is the dominant mechanism of solute transport of loess soils in the study area.

关键词： solute transport loess soil pore-water velocity dispersion coefficient hydraulic conductivity Loess Plateau

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：