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Sequencing of p53 mutation in established human hepatocellular carcinoma cell line of HHC4 and HHC15 in nude mice

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World Journal of Gastroenterology 1998年第6期4卷 51-55页

作者： yanG Shan Min, ZHOU Hong, CHEN Rui Chuan, WANG Yu Fang, CHEN Fu, ZHANG Chang Gong, ZHEN Yun, yan Jiang hua and su Jin hua

AIM To set up cell lines of human hepatocellular carcinoma in nude mice for the research of cell biology and gene therapy. METHODS Xenotransplantation of human hepatoma into nude mice was carried out and the growth rate, histopathology and immunology of the nude mice were studied. The DNA from xenografts were analyzed by HBV gene and PCR amplification of a fragment of p 53 gene exon 7, which were identified by dot blot hybridization, restriction fragments length polymorphism and DNA sequencing. REsuLTS hHCC4 and hHCC415 cell lines could be successively transplanted in nude mice and the population doubling time was 7 and 5 days respectively. These strains retained the original characteristics of histopathology, secreting AFP and heteroploid karyotypes in human hepatocellular carcinoma. The fragment of HBV gene was detected in the genomic DNA of both hHCC4 and hHCC15, however only hHCC4 secreted HBsAg. The mutation at 250 code (C→A) and 249 code (G→T) were detected respectively in the genomic DNA of hHCC4 and hHCC15. CONCLUSION The two cell lines are useful material for studying cell biology and gene therapy in human hepatocellular carcinoma and provide molecular biological trace of the relationship between high mortality of hepatoma and AFB1 severe pollution of the daily common foods in this district.

关键词： Liver neoplasms carcinoma, hepatocellular p53 gene mutation HHC4 HHC15 Tumor cell, cultured

High-continuity genome assembly of the jellyfish Chrysaora quinquecirrha

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Zoological Research 2021年第1期42卷 130-134页

作者： Wang-Xiao Xia Hao-Rong Li Jing-Hao Ge Yao-Wu Liu Hong-Hui Li yan-hua su Hai-Zhen Wang Hui-Fang Guo Yu-Xuan Dai Yao-Wen Liu Xing-Chun Gou Shaanxi Key Laboratory of Brain Disorders Institute of Basic Translational MedicineXi’an Medical UniversityXi’anShaanxi 710021China Key Laboratory of Animal Gene Editing and Animal Cloning in Yunnan Province Yunnan Agricultural UniversityKunmingYunnan 650201China Center for Ecological and Environmental Sciences Northwestern Polytechnical UniversityXi’anShaanxi 710072China ZhiQiao Research Institute ChangshaHunan 410000China Shaanxi Key Laboratory of Infection and Immune Disorders School of Basic Medical ScienceXi’an Medical UniversityXi’anShaanxi 710021China

DEAR EDITOR,The Atlantic sea nettle(Chrysaora quinquecirrha)has an important evolutionary position due to its high ecological ***,due to limited sequencing technologies and complex jellyfish genomic sequences,the current *** genome assembly is highly ***,we used the most advanced high-throughput chromosome conformation capture(Hi-C)technology to obtain high-coverage sequencing data of the *** *** then anchored these data to the previously published contig-level assembly to improve the ***,a high-continuity genome sequence of *** was successfully assembled,which contained 1882 scaffolds with a N50 length of 3.83 *** N50 length of the genome assembly was 5.23 times longer than the previously released one,and additional analysis revealed that it had a high degree of genomic continuity and *** of the high-continuity genome sequence of *** not only provides a basis for the study of jellyfish evolution through comparative genomics but also provides an important resource for studies on jellyfish growth and development.

关键词： fish continuity assembly

Glucocorticoid receptor signaling in the brain and its involvement in cognitive function

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Neural Regeneration Research 2025年第9期20卷 2520-2537页

作者： Chonglin su Taiqi huang Meiyu Zhang yanyu Zhang yan Zeng Xingxing Chen Brain Science and Advanced Technology Institute Hubei Province Key Laboratory of Occupational Hazard Identification and ControlSchool of MedicineWuhan University of Science and TechnologyWuhanHubei ProvinceChina

The hypothalamic-pituitary-adrenal axis regulates the secretion of glucoco rticoids in response to environmental *** the brain,a nuclear receptor transcription fa ctor,the glucocorticoid recepto r,is an important component of the hypothalamicpituitary-a d renal axis's negative feedback loop and plays a key role in regulating cognitive equilibrium and *** glucoco rticoid receptor influences cognitive processes,including glutamate neurotransmission,calcium signaling,and the activation of brain-derived neurotrophic factor-mediated pathways,through a combination of genomic and non-genomic *** interactions within the central nervous system can alter the expression and activity of the glucocorticoid receptor,there by affecting the hypothalamic-pituitary-a d renal axis and stress-related cognitive *** appropriate level of glucocorticoid receptor expression can improve cognitive function,while excessive glucocorticoid receptors or long-term exposure to glucoco rticoids may lead to cognitive *** with cognitive impairment-associated diseases,such as Alzheimer's disease,aging,depression,Parkinson's disease,Huntington's disease,stroke,and addiction,often present with dysregulation of the hypothalamic-pituitary-adrenal axis and glucocorticoid receptor *** review provides a comprehensive overview of the functions of the glucoco rticoid receptor in the hypothalamic-pituitary-a d renal axis and cognitive *** emphasizes that appropriate glucocorticoid receptor signaling fa cilitates learning and memory,while its dysregulation can lead to cognitive *** provides clues about how glucocorticoid receptor signaling can be targeted to ove rcome cognitive disability-related disorders.

关键词： brain-derived neurotrophic factor calcium signaling glucocorticoid receptor glucocorticoid glutamate transmission hypothalamic-pituitary-adrenal axis long-term potentiation neurocognitive disorders neuroplasticity stress

Pathogenesis, diagnosis, and treatment of epilepsy: electromagnetic stimulation-mediated neuromodulation therapy and new technologies

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Neural Regeneration Research 2025年第4期20卷 917-935页

作者： Dian Jiao Lai Xu Zhen Gu hua yan Dingding Shen Xiaosong Gu Academy of Medical Engineering and Translational Medicine Tianjin UniversityTianjinChina Key Laboratory of Advanced Drug Delivery Systems of Zhejiang Province College of Pharmaceutical SciencesZhejiang UniversityHangzhouZhejiang ProvinceChina Affiliated Hospital of Nantong University NantongJiangsu ProvinceChina

Epilepsy is a severe,relapsing,and multifactorial neurological *** regarding the accurate diagnosis,prognosis,and in-depth pathogenesis are crucial for the precise and effective treatment of *** pathogenesis of epilepsy is complex and involves alterations in variables such as gene expression,protein expression,ion channel activity,energy metabolites,and gut microbiota *** results are lacking for conventional treatments for *** resection of lesions,drug therapy,and non-drug interventions are mainly used in clinical practice to treat pain associated with ***-pharmacological treatments,such as a ketogenic diet,gene therapy for nerve regeneration,and neural regulation,are currently areas of research *** review provides a comprehensive overview of the pathogenesis,diagnostic methods,and treatments of *** also elaborates on the theoretical basis,treatment modes,and effects of invasive nerve stimulation in neurotherapy,including percutaneous vagus nerve stimulation,deep brain electrical stimulation,repetitive nerve electrical stimulation,in addition to non-invasive transcranial magnetic stimulation and transcranial direct current *** studies have shown that electromagnetic stimulation-mediated neuromodulation therapy can markedly improve neurological function and reduce the frequency of epileptic ***,many new technologies for the diagnosis and treatment of epilepsy are being ***,current research is mainly focused on analyzing patients’clinical manifestations and exploring relevant diagnostic and treatment methods to study the pathogenesis at a molecular level,which has led to a lack of consensus regarding the mechanisms related to the disease.

关键词： diagnosis drug treatment electroencephalography epilepsy monitoring epilepsy nerve regeneration neurostimulation non-drug interventions pathogenesis prediction

Effectiveness of potential antiviral treatments in COVID-19 transmission control:a modelling study

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Infectious Diseases of Poverty 2021年第2期10卷 90-91页

作者： Sheng-Nan Lint Jia Rui Qiu-Ping Chen Bin Zhao Shan-Shan Yu Zhuo-yang Li Ze-Yu Zhao Yao Wang Yuan-Zhao Zhu Jing-Wen Xu Meng yang Xing-Chun Liu Tian-Long yang Li Luo Bin Deng Jie-Feng huang Chan Liu Pei-hua Li Wei-Kang Liu Fang Xie Yong Chen yan-hua su Ben-hua Zhao Yi-Chen Chiang Tian-Mu Chen State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics School of Public HealthXiamen University4221-117 South Xiang’an RoadXiang’anXiamenFujianPeople’s Republic of China 不详

Background:Novel coronavirus disease 2019(COVID-19)causes an immense disease *** public health countermeasures effectively controlled the epidemic in China,non-pharmaceutical interventions can neither be maintained indefinitely nor conveniently implemented *** is mainly used to prevent COVID-19,and most current antiviral treatment evaluations focus on clinical ***,we conducted population-based simulations to assess antiviral treatment effectiveness among different age groups based on its clinical efficacy.

关键词： COVID-19 Antiviral treatment Age group Transmission model

Feasibility of controlling hepatitis E in Jiangsu Province, China: a modelling study

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Infectious Diseases of Poverty 2021年第3期10卷 116-117页

作者： Meng yang Xiao-Qing Cheng Ze-Yu Zhao Pei-hua Li Jia Rui Sheng-Nan Lin Jing-Wen Xu Yuan-Zhao Zhu Yao Wang Xing-Chun Liu Li Luo Bin Deng Chan Liu Jie-Feng huang Tian-Long yang Zhuo-yang Li Wei-Kang Liu Wen-Dong Liu Ben-hua Zhao Yue He Qi Yin Si-Ying Mao yan-hua su Xue-Feng Zhang Tian-Mu Chen State Key Laboratory of Molecular Vacci no logy and Molecular Diagnostics School of Public HealthXiamen University4221-117 South Xiang'an RoadXiang'an DistrictXiamen City 361102Fujian ProvincePeople's Republic of China Jiangsu Center for Disease Control and Prevention Nanjing CityJiangsu ProvincePeople's Republic of China

Background:Hepatitis E,an acute zoonotic disease caused by the hepatitis E virus(HEV),has a relatively high burden in developing *** current research model on hepatitis E mainly uses experimental animal models(such as pigs,chickens,and rabbits)to explain the transmission of *** studies have developed a multi-host and multiroute transmission dynamic model(MHMRTDM)to explore the transmission feature of ***,this study aimed to explore its transmission and evaluate the effectiveness of intervention using the dataset of Jiangsu Province.

关键词： Hepatitis E Transmission dynamic model Meteorological factor Intervention Transmissibility

416例放置宫内节育器(IUD)出血的相关因素探讨

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现代预防医学 2010年第23期37卷 4446-4447页

作者：王玎颜素华湖北省宜昌市三峡大学仁和医院妇产科

[目的]探讨放置宫内节育器出血的相关因素。[方法]对某院2003年1月~2006年12月已经确诊的416例放置宫内节育器出血的临床资料进行回顾性分析。[结果]金属单环、宫形器。塑料IUD出血的发生率为57.78%,56.89%;明显高于活性以含铜为主的IUD... 详细信息

[目的]探讨放置宫内节育器出血的相关因素。[方法]对某院2003年1月~2006年12月已经确诊的416例放置宫内节育器出血的临床资料进行回顾性分析。[结果]金属单环、宫形器。塑料IUD出血的发生率为57.78%,56.89%;明显高于活性以含铜为主的IUD10.83%(P﹤0.01);IUD出血与细菌、CT、Mg的感染密切相关,其检出率分别为35%左右、25%左右、22%左右,同时与经期卫生不良及不注意性交卫生和长期精神紧张有关。并且其出血常发生在放置IUD 3~5年内。[结论]放置宫内节育器出血的相关因素与经期卫生不良和不注意性交卫生加大病原体的入侵机会,导致慢性迁延性病变,使子宫内膜不规则出血;长期精神紧张致神经内分泌调节功能紊乱而致子宫内膜不规则出血;另外,异物排斥反应过程可能与子宫内膜出血发生时间有关。

关键词：宫内节育器(IUD) 出血相关因素

Curcumin protects mitochondria from oxidative damage and attenuates apoptosis in cortical neurons

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Acta Pharmacologica Sinica 2004年第12期25卷 40-46页

作者： Yuan-gui ZHU2, Xiao-chun CHEN3, Zhi-zhe CHEN4, Yu-qi ZENG, Guang-bin SHI, yan-hua su, Xu PENG Fujian Instituteof Geriatrics, Fujian Institute of Hematology, Union Hospital, Fujian Medical University, Fuzhou 350001, China 2Now in Rush University Medical Center, Chicago, IL 60612,USA. Fujian Institute of Geriatrics Union Hospital Fujian Medical University Fuzhou 350001 Fujian Institute of Hematology Union Hospital Fujian Medical University Fuzhou 350001

AIM: To investigate the effect of curcumin on tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in rat cortical neurons and to explore the possible mechanism. METHODS: Primary cultured rat cortical neurons were performed in vitro and cell viability was measured by MTT assay. DNA fragmentation was used to evaluate cell apoptosis. Intracellular reactive oxygen species (ROS) and mitochondrial membrane potential (?Ψm) was deter- mined by flow cytometric assay. Cellular glutathione (GSH) content was measured by spectrophotometer. Bcl-2 family proteins, cytochrome c, cleaved caspase-3, and poly (ADP-ribose) polymerase (PARP) were detected by Western blot. REsuLTS: Exposure of tBHP 100 μmol/L to neurons for 60 min resulted in ?Ψm loss and cyto- chrome c release from mitochondria and subsequent activationof caspase-3 and PARP cleavation, and cell apoptosis. After removal of tBHP and then further treatment with curcumin (2.5-20 μmol/L) for 18 h, curcumin abrogated ?Ψm loss and cytochrome c release, blocked activation of caspase3, and altered the expression of Bcl-2 family. Further curcumin treatment also prevented cellular GSH and decreased intracellular ROS generation markedly. Curcumin eventually attenuated tBHP-induced apoptosis in cortical neurons. CONCLUSION: Curcumin may attenuate oxidative damages in cortical neurons by reducing intracellular production of ROS and protecting mito- chondria from oxidative damage.

关键词： Curcumin tert-butyl hydroperoxide apoptosis oxidative stress Bcl-2 family proteins mitochondria Alzheimerdisease

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论晚清民国时期战争对当铺的影响

佳木斯职业学院学报 2016年第8期32卷 82-83页

作者：闫素华渤海大学政治与历史学院辽宁锦州121000

随着鸦片战争的爆发,我国被迫打开了国门,之后在政治经济文化等各个方面都对我国造成了很大影响,在经济上更是冲击了我国传统的小农经济,本文以典当业为例,试论晚清民国时期战争对典当业所造成的影响。

关键词：典当业晚清民国影响融资困难