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Dot immunogold filtration assay for rapid detection of anti-HAV IgM in Chinese

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World Journal of Gastroenterology 2000年第3期6卷 400-401页

作者： Han FC Hou Y yan xj Xiao LY Guo YH Fourth Mil Med Univ Chinese PLA Inst Gene Diag Xian 710033 Peoples R China

INTRODUCTION The hepatitis A virus specific immunoglobulin M(IgM)antibody is a specific serological marker forearly diagnosis of hepatitis A..At present,themethods used at home or abroad for detecting anti-HAV IgM are RIA,ELISA and *** dotimmunogold combination assay that has beendeveloped since 1989 is a new technique with theproperty of simple and rapid immunologicaldetection,by using the red colloidal gold particles tolabel the antibodies as indicator,and the

关键词： dot immunogold filtration assay hepatitis A virus immunoglobulin M/analysis

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短片段一步法RT-PCR方法的建立

第四军医大学学报 2001年第4期22卷 375-376页

作者：郭晏海闫小君崔大祥侯瑜苏成芝第四军医大学全军基因诊断技术研究所陕西西安710033

0 引言传统RT-PCR方法需要用反转录酶将RNA先反转录成cDNA，然后用Taq酶对cDNA进行PCR扩增，而且往往需要巢式PCR扩增才能提高结果的特异性和灵敏度，操作比较麻烦. 有人发现Taq DNA聚合酶有一定的反转录活性［1］，可以实现只用Taq DN... 详细信息

0 引言传统RT-PCR方法需要用反转录酶将RNA先反转录成cDNA，然后用Taq酶对cDNA进行PCR扩增，而且往往需要巢式PCR扩增才能提高结果的特异性和灵敏度，操作比较麻烦. 有人发现Taq DNA聚合酶有一定的反转录活性［1］，可以实现只用Taq DNA聚合酶进行反转录的单反应管一步法RT-PCR扩增. 但是我们在实验过程中发现，利用Taq DNA聚合酶进行一步法RT-PCR反应成功率往往不高，这可能是因为Taq DNA聚合酶的反转录活性不高的原因造成的. 为此，我们重新设计了实验方法，以提高用Taq DNA聚合酶进行RT-PCR的成功率.

关键词： RT-PCR 一步法聚合酶链反应 CDNA RNA

Research on the mechanism of rockburst induced by mined coal-rock linkage of sharply inclined coal seams

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International Journal of Minerals,Metallurgy and Materials 2024年第5期31卷 929-942页

作者： Xingping Lai Huicong Xu Pengfei Shan Qinxin Hu Weixi Ding Shangtong yang Zhongming yan College of Energy Science and Engineering Xi’an University of Science and TechnologyXi’an 710054China State Key Laboratory of Green Low Carbon Development of Oil-rich Coal in Western China Xi’an 710054China College of Future Technology Xi’an University of Science and TechnologyXi’an 710054China Department of Civil and Environmental Engineering University of StrathclydeGlasgowG11XJUK

In recent years,the mining depth of steeply inclined coal seams in the Urumqi mining area has gradually *** deformation of mining coal-rock results in frequent *** has become a critical issue that affects the safe mining of deep,steeply inclined coal *** this work,we adopt a perspective centered on localized deformation in coal-rock mining and systematically combine theoretical analyses and extensive data mining of voluminous microseismic *** describe a mechanical model for the urgently inclined mining of both the sandwiched rock pillar and the roof,explaining the mechanical response behavior of key disaster-prone zones within the deep working face,affected by the dynamics of deep *** exploring the spatial correlation inherent in extensive microseismic data,we delineate the“time-space”response relationship that governs the dynamic failure of coal-rock during the progression of the sharply inclined working *** results disclose that(1)the distinctive coal-rock occurrence structure characterized by a“sandwiched rock pillar-B6 roof”constitutes the origin of rockburst in the southern mining area of the Wudong Coal Mine,with both elements presenting different degrees of deformation localization with increasing mining depth.(2)As mining depth increases,the bending deformation and energy accumulation within the rock pillar and roof show nonlinear *** localized deformation of deep,steeply inclined coal-rock engenders the spatial superposition of squeezing and prying effects in both the strike and dip directions,increasing the energy distribution disparity and stress asymmetry of the“sandwiched rock pillar-B3+6 coal seam-B6 roof”*** makes worse the propensity for frequent dynamic disasters in the working face.(3)The developed high-energy distortion zone“inner-outer”control technology effectively reduces high stress concentration and energy distortion in the surrounding *** implementation,the averag

关键词： steeply inclined coal seam localized deformation mechanism of induced rockburst prevention and control of rockburst

Modeling and analysis of gradient metamaterials for broad fusion bandgaps

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Applied Mathematics and Mechanics(English Edition) 2024年第7期45卷 1155-1170页

作者： Changqi CAI Chenjie ZHU Fengyi ZHANG Jiaojiao SUN Kai WANG Bo yan Jiaxi ZHOU School of Mechanical Engineering Zhejiang Sci-Tech UniversityHangzhou310018China School of Material and Environmental Engineering Hangzhou Dianzi UniversityHangzhou310018China College of Mechanical and Vehicle Engineering Hunan UniversityChangsha410082China

A gradient metamaterial with varying-stiffness local resonators is proposed to open the multiple bandgaps and further form a broad fusion ***,three local resonators with linearly increasing stiffness are periodically attached to the spring-mass chain to construct the gradient *** dispersion relation is then derived based on Bloch's theorem to reveal the fusion bandgap *** dynamic characteristic of the finite spring-mass chain is investigated to validate the fusion of multiple ***,the effects of the design parameters on multiple bandgaps are *** results show that the metamaterial with a non-uniform stiffness gradient pattern is capable of opening a broad fusion bandgap and effectively attenuating the longitudinal waves within a broad frequency region.

关键词： local resonance mechanism elastic metamaterial stiffness gradient bandgap fusion broadband wave attenuation

Quantum squeezing induced nonreciprocal phonon laser

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Science China(Physics,Mechanics & Astronomy) 2024年第6期67卷 20-30页

作者： Tian-Xiang Lu yan Wang Keyu Xia Xing Xiao Le-Man Kuang Hui Jing College of Physics and Electronic Information Gannan Normal UniversityGanzhou 341000China Key Laboratory of Low-Dimensional Quantum Structures and Quantum Control of Ministry of Education Department of Physics and Synergetic Innovation Center for Quantum Effects and ApplicationsHunan Normal UniversityChangsha 410081China Academy for Quantum Science and Technology Zhengzhou University of Light IndustryZhengzhou 450002China College of Engineering and Applied Sciences National Laboratory of Solid State Microstructuresand Collaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing 210023China

Phonon lasers or coherent amplifications of mechanical oscillations are powerful tools for fundamental studies on coherent acoustics and hold potential for diverse applications,ranging from ultrasensitive force sensing to phononic information ***,we propose the use of an optomechanical resonator coupled to a nonlinear optical resonator for directional phonon *** find that by pumping the nonlinear optical resonator,directional optical squeezing can occur along the pump *** a result,we can achieve the directional mechanical gain using directional optical squeezing,thereby leading to nonreciprocal phonon lasing with a well-tunable directional power *** work proposes a feasible way to build nonreciprocal phonon lasers with various nonlinear optical media,which are important for a wide range of applications,such as directional acoustic amplifiers,invisible sound sensing or imaging,and one-way phononic networks.

关键词： nonreciprocal phonon laser directional quantum squeezing optomechanics

Astronomical influence of the development of Paleogene thin coal seam groups in offshore Lacustrine basins:A case study of the ZhuⅠDepression's Enping Formation located in the northern South China Sea

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Acta Oceanologica Sinica 2024年第4期43卷 136-150页

作者： yan Liu Shengbing Huang Dongdong Wang Nan Li Yuting Yin Ying Chen Zengxue Li College of Earth Science and Engineering Shandong University of Science and TechnologyQingdao 266590China CNOOC Research Institute Co. Ltd.Beijing 100028China School of Geography Earth and Environmental SciencesUniversity of BirminghamBirmingham B152TTUK

The development of the Paleogene coal seams in China's offshore basin areas generally had the characteristics of coal measures with large thicknesses,large numbers of coal seams,thin single coal seams,poor stability,scattered vertical distribution,and a wide distribution *** study selected the Enping Formation of the ZhuⅠDepression in the northern section of the South China Sea as an example to determine the macro-control factors of the development of the Paleogene coal seam *** analysis was carried out on the influencing effects and patterns of the astronomical cycles related to the development of the thin coal seam groups in the region.A floating astronomical time scale of the Enping Formation was established,and the sedimentary time limit of the Enping Formation was determined to be approximately 6.15 Ma±.In addition,the cyclostratigraphy analysis results of the natural gamma-ray data of Well xj in the Enping Formation of the Xijiang Sag revealed that the development of the thin coal seams had probably been affected by short eccentricity and precession *** formation process of coal seams was determined to have been affected by high seasonal contrast,precipitation,and *** the periods with high values of short eccentricity,the seasonal contrasts tended to be *** those periods,fluctuations in the precession controls resulted in periodic volume changes in precipitation and insolation of the region,resulting in the development of thin coal *** was also found that the periods with low precession were the most conducive to coal seam *** that basis,combined with such factors as sedimentary environmental conditions conducive to the development of thin coal seam groups,this study established a theoretical model of the comprehensive influences of short eccentricity and precession on the development and distribution of Paleogene thin coal seam groups in offshore lacustrine *** patterns of the Paleogene astrono

关键词： Paleogene Pearl(Zhujiang)River Mouth Basin coal seam development astronomical cycles

Lindqvist-type Polyoxometalates Act as Anti-breast Cancer Drugs via Mitophagy-induced Apoptosis

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Current Medical Science 2024年第4期44卷 809-819页

作者： Chen-guang YAO Zi-jia ZHAO Ting TAN Jiang-ning yan Zhong-wei CHEN Jun-tao XIONG Han-luo LI yan-hong WEI Kang-hong HU Sino-German Biomedical Center Hubei Provincial Key Laboratory of Industrial MicrobiologyCooperative Innovation Center of Industrial Fermentation(Ministry of Education&Hubei Province)Key Laboratory of Fermentation Engineering(Ministry of Education)National“111”Center for Cellular Regulation and Molecular PharmaceuticsHubei University of TechnologyWuhan430068China Center for Evaluation of Hubei Medical Products Administration Wuhan430068China

Objective Lindqvist-type polyoxometalates(POMs)exhibit potential antitumor *** study aimed to examine the effects of Lindqvist-type POMs against breast cancer and the underlying *** Using different cancer cell lines,the present study evaluated the antitumor activities of POM analogues that were modified at the body skeleton based on molybdenum-vanadium-centered negative oxygen ion polycondensations with different side *** colony formation assay,autophagy detection,mitochondrial observation,qRT-PCR,Western blotting,and animal model were used to evaluate the antitumor activities of POMs against breast cancer cells and the related *** MO-4,a Lindqvist-type POM linking a proline at its side strain,was selected for subsequent experiments due to its low half maximal inhibitory concentration in the inhibition of proliferation of breast cancer *** was found that MO-4 induced the apoptosis of multiple types of breast cancer ***,MO-4 activated intracellular mitophagy by elevating mitochondrial reactive oxygen species(ROS)levels and resulting in *** vivo,breast tumor growth and distant metastasis were significantly reduced following MO-4 *** Collectively,the results of the present study demonstrated that the novel Lindqvist-type POM MO-4 may exhibit potential in the treatment of breast cancer.

关键词： antitumor breast cancer polyoxometalate mitochondria mitophagy

Prevalence,Characteristics and Treatment of Knee Osteoarthritis in Urban Residents of Beijing,China:A Community-Based Cross-Sectional Study

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Biomedical and Environmental Sciences 2024年第4期37卷 418-422页

作者： ZHOU Jun yan yan DONG Ya Wei LIU Xiao Yu WANG Ping LIN Jia Ming DONG Peng Xuan QUAN Rui WANG Rong Tian MI Bao Hong CHEN Wei Heng Beijing University of Chinese Medicine Third Affiliated Hospital Beijing 100029China Engineering Research Center of Chinese Orthopaedics and Sports Rehabilitation Artificial Intelligent Ministry of EducationBeijing 100029China Beijing Hepingli Community Health Service Center Beijing 100013China Minimally Invasive Joint Surgery Beijing University of Chinese Medicine Third Affiliated HospitalBeijing 100029China

Knee osteoarthritis(KOA)is a common progressive joint disease with chronic pain and movement disorders as the main clinical *** is a major public health problem worldwide and it imposes serious medical and economic *** accounts for nearly four-fifths of the global Osteoarthritis burden and increases with rising obesity and age[1].There are many reasons for the change in disease prevalence and risk factors,such as urbanization,lifestyle changes,population aging,and sex imbalances.

关键词： Osteoarthritis clinical obesity

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肺源性心脏病急性发作期免疫功能的改变

中国组织工程研究与临床康复 2007年第12期11卷 2345-2347页

作者：段世芳戴爱国张平王立忠胡耀明颜向军唐国华李强翔南华大学附属第一医院内科南华大学附属第一医院检验科湖南省衡阳市421001 南华大学科研实验中心湖南省衡阳市421001 中南大学湘雅医院内分泌科湖南省长沙市410008

目的:观察肺源性心脏病(简称肺心病)急性发作期患者免疫功能的变化。方法:选择南华大学附属第一医院2004-11/2006-01收治慢性肺心病急性发作期患者60例为肺心病组,于急性加重期入院第2天7:00,空腹抽取静脉血,采用流式细胞仪检测T细胞亚... 详细信息

目的:观察肺源性心脏病(简称肺心病)急性发作期患者免疫功能的变化。方法:选择南华大学附属第一医院2004-11/2006-01收治慢性肺心病急性发作期患者60例为肺心病组,于急性加重期入院第2天7:00,空腹抽取静脉血,采用流式细胞仪检测T细胞亚群CD3+、CD4+、CD8+及自然杀伤细胞活性,免疫浊度法检测体液免疫指标(IgG,IgM,IgA及补体C3)。以同期60例健康体检者为对照。结果:120例是否受试者均进入结果分析。①T细胞亚群:肺心病组CD3+,CD4+水平低于对照组(0.52±0.06,0.62±0.04;0.32±0.06,0.41±0.06;P均<0.05),CD4+/CD8+高于对照组(1.96±0.26,1.84±0.78,P<0.05)。②免疫血清指标:肺心病组IgA、补体C3及自然杀伤细胞活性低于对照组[(1.26±0.74),(2.45±0.85)g/L;(6200±217),(9960±302)mg/L;0.34±0.08,0.57±0.07;P均<0.05]。结论:肺源性心脏病急性发作期患者的细胞免疫和体液免疫功能均受损,尤以细胞免疫功能受损更突出,且与病情呈平行关系。

关键词：肺心病 T细胞亚群免疫球蛋白