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Studies on the Phase Properties of Winsor Ⅰ-Ⅲ Type Microemulsions with Dielectric Relaxation Spectroscopy

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Chinese Chemical Letters 2005年第9期16卷 1263-1266页

作者： Jin Ling CHAI yan hong gao Kong Shuang ZHAO Gan Zuo LI gao Yong ZHANG Department of Chemistry Shandong Normal University Jinan 250014 Key Laboratory for Colloid and Interface Chemistry of State Education Ministry Shandong University Jinan 250100 Department of Chemistry.Beijing Normal University Beijing 100875

The dielectric relaxation spectroscopy （DRS） of Winsor I -Ⅲ microemulsions for nonionic surfactant octyl polyglucoside CsG1.46/1-butanol/cyclohexanedwater system was studied. The experiment shows that the permittivity decreases with the increase in the frequency and clear dielectric relaxation phenomena were observed. Permittivity obviously decreases with the change of the microstructure of the microemulsion, W/O, B.C. and O/W can be distinguished by the permittivity.

关键词： Phase property microemulsion dielectric relaxation spectroscopy

Effects of cultivation conditions on the diversity of microbes involved in the conversion of rice straw to fodder

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Journal of Environmental Sciences 2007年第1期19卷 67-73页

作者： yanG hong-yan gao Li-juan WANG Xiao-fen WANG Wei-dong CUI Zong-jun College of Agronomy and Biotechnology China Agricultural University Beijing 100094 China

To confirm the optimum cultivation conditions for analyzing lactic acid bacterial communities and to provide the cultivation foundation for lactic acid bacterial communities that were used to convert straw into fodder, fermented rice straw was inoculated into 13 different broths. After 48 h of cultivation, pH values, volatile products, and microbial diversity were analyzed. Except for LAB broth, the pH values of the other broths could decrease to approximately 4.5. GC/MS analysis showed that lactic acid in Tomato MRS broth, MRS broth, LAB broth, and Tomato juice broth was higher than that in the other broths. DNA concentration analysis showed that the counts of microbes in Tomato MRS broth were 2.5 times of those in other broths and that tomato juice favored the reproduction of the microbes. Denaturing gradient gel electrophoresis （DGGE） analysis showed that the number of lactic acid bacterial species in HYA broth, Tomato juice broth, and Tomato MRS broth were higher than those in the other broths.

关键词： straw cultivation condition microbial diversity DGGE

Isolation and Identification of Virus dsRNA from Strawberry Plants

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Agricultural Sciences in China 2007年第1期6卷 86-93页

作者： LI He DAI hong-yan ZHANG Zhi-hong gao Xiu-yan DU Guo-dong ZHANG Xin-yu College of Horticulture Shenyang Agricultural University Shenyang 110161 P.R.China

The analysis of virus genome is based on nucleic acid isolation. The aims of this study were to develop a method for isolation and identification of virus double-stranded ribonucleic acid （dsRNA） and to elucidate the nucleotide sequences of strawberry virus. Using the modified method, virus dsRNA was extracted from strawberry virus indicator plants and cultivated strawberry plants and detected using agarose gel electrophoresis with ethidium bromide staining and reverse transcription-polymerase chain reaction （RT-PCR）. The quantity of virus dsRNA varied among strawberry cultivars. The quantity of dsRNA from in vitro plantlets was higher than that from the young leaves of field plants. For the field-grown plants, there was more dsRNA in the young leaves. Virus dsRNA extracted from strawberry plants was resistant to deoxyribonuclease Ⅰ （DNase Ⅰ）, but evidently, it became resistant to ribonuclease A （RNase A） only in the presence of 0.5 M NaCl. Its bands in agarose gel could be readily recycled using an agarose gel DNA purification kit. With RT-PCR, the segments of both strawberry mottle virus and Strawberry mild yellow edge virus genomes were amplified by using the virus dsRNA recycled from gel or treated with DNase Ⅰ /RNase A as templates. The system developed for dsRNA isolation and identification in strawberry plants laid a sound foundation for the work on genome analysis of strawberry virus isolates in China.

关键词： dsRNA virus strawberry isolation RT-PCR

The Target of Benzimidazole Carbamate Against Cysticerci cellulosae

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Agricultural Sciences in China 2007年第8期6卷 1009-1017页

作者： LI Qing-zhang HAO yan-hong gao Xue-jun gao Wen-xue ZHAO Bing College of Biological Science Northeast Agricultural University Harbin 150030 P.R.China

To study the target of benzimidazole carbamate drugs against Cysticerci cellulosae and give a theoretical basis for type evolution and new drug design, the changes of key enzyme activities and metabolite contents in the pathway of energy metabolism in C. cellulosae in vitro and in vivo were tested with albendazole and oxfendazole, respectively. Both albendazole and oxfendazole inhibited the pathways of anaerobic glycolysis, partial inversed tricarboxylic acid cycle of Taenia Solium oncosphere, immature and mature Cysticerci in vitro, and immature and mature Cysticerci in vivo to a certain degree, and enhanced fat decomposing, amino acid decomposing, xanthine decomposing metabolism, and on the other hand, the absorption of glucose was hindered; furthermore, both albendazole and oxfendazole inhibited the activities of the fumaric reductase （FR） complex noncompetently in vitro. Benzimidazole carbamate drugs could inhibit the activities of FR complex noncompetently and hinder the absorption of glucose.

关键词： albendazole oxfendazole Cysticerci cellulosae target energy metabolism

Rapid determination of C1^- by light-absorption ratio variation approach using Cl^--eosin Y-Ag^+ adsorptive precipitation

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Chinese Chemical Letters 2008年第5期19卷 569-572页

作者： Jing Bai hong yan Wang hong Wen gao State Key Laboratory of Pollution Control and Resource Reuse College of Environmental Science and Engineering Tongji University Shanghai 200092 China

The adsorptive precipitation between eosin Y （EY） and AgCl colloids at pH 3.73 caused the sensitive color change of the solution. The reaction mechanism between EY and AgCl was analyzed and this reaction was used fo... 详细信息

关键词： Determination of chloride Eosin Y Adsorptive precipitation Light-absorption ratio variation approach

Tomato Fruit Development and Ripening Are Altered by the Silencing of LeEIN2 Gene

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Journal of Integrative Plant Biology 2006年第12期48卷 1478-1485页

作者： hong-Liang Zhu Ben-Zhong Zhu Yi Shao Xiao-Guang Wang Xi-Jin Lin Yuan-hong Xie Ying-Cong Li hong-yan gao Yun-Bo Luo Laboratory of Fruit Biology College of Food Science and Nutritional Engineering China Agricultural University Beijing 100083 China

Loss-of-function ethylene insensitive 2 （EIN2） mutations showed ethylene insensitivity in Arabidopsis, which indicated an essential role of EIN2 in ethylene signaling. However, the function of EIN2 in fruit ripening has not been investigated. To gain a better understanding of EIN2, the temporal regulation of LeEIN2 expres- sion during tomato fruit development was analyzed. The expression of LeEIN2 was constant at different stages of fruit development, and was not regulated by ethylene. Moreover, LeEIN2-silenced tomato fruits were developed using a virus-induced gene silencing fruit system to study the role of LeEIN2 in tomato fruit ripening. Silenced fruits had a delay in fruit development and ripening, related to greatly descended expression of ethylene-related and ripening-related genes in comparison with those of control fruits. These results suggested LeEIN2 positively mediated ethylene signals during tomato development. In addition, there were fewer seeds and Iocules in the silenced fruit than those in the control fruit, like the phenotype of parthenocarpic tomato fruit. The content of auxin and the expression of auxin-regulated gene were declined in silenced fruit, which indicated that EIN2 might be important for crosstalk between ethylene and auxin hormones.

关键词： ethylene fruit development LeEIL1 LeEIN2 tomato virus-induced gene silencing

Preparation of Artificial Antigen and Egg Yolk-derived Immunoglobulin(IgY) of Citrinin for Enzyme-Linked Immunosorbent Assay

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Biomedical and Environmental Sciences 2009年第3期22卷 237-243页

作者： ZHAO-HUI DUAN ZHUANG-SEN LIN HE-RUI YAO yan-hong gao KUN ZHANG Su-QING ZHAO ZHEN-YU ZHU Laboratory of Clinical Immunology the Second Affiliated Hospital of Sun Yat-Sen University Guangzhou 510120 Guangdong China Department of Pharmacy Engineering Faculty of Chemical Engineering and Light Industry Guangdong University of Technology Guangzhou 510006 Guangdong China Guangdong Center for Disease Control and Prevention Guangzhou 510300 Guangdong China Department of Biochemistry Zhongshan Medical College Sun Yat-Sen University Guangzhou 510080 Guangdong China

Objective To prepare artificial antigens and anti-citrinin egg yolk-derived immunoglobulin （IgY） to build an enzyme-linked immunosorbent assay （ELISA） for citrinin （CTN）. Methods CTN was conjugated with bovine serum albumin （BSA）, ovalbumin （OVA） with formaldehyde condensation method to prepare artificial antigens and identified by ultraviolet （UV） spectrometry and Infrared （IR） spectrometry. Artificial antigens for CTN and anti-CTN IgY were purified with polyethylene glycol two-step precipitation method and identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. ELISA with IgY was established. Cross-reactivity of IgY with various structural similarities to CTN and possible co-occurrence with CTN in agricultural commodities were studied. Results UV and IR absorption spectra suggested that CTN was correlated with the carrier protein of BSA or OVA. SDS-PAGE patterns showed that the anti-CTN IgY was almost pure with a molecular weight of approximate 100 KD. The indirect competitive ELISA showed that the detection limit of CTN was 10 ng·mL^-1, with a good linearity ranging 20-640 ng·mL^-1. Conclusion Artificial antigens of CTN can be successfully synthesized. The established ELISA can be used to determine CTN- contaminated samples.

关键词： Citrinin Artificial antigen lgY ELISA

Computer-aided recognition and assessment of a porous bioelastomer in ultrasound images for regenerative medicine applications

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Medicine in Novel Technology and Devices 2023年第3期19卷 41-52页

作者： Dun Wang Sheng yang Kai-Xuan Guo yan-Ying Zhu Jia Sun Aliona Dreglea yan-hong gao Jiao Yu College of Science Liaoning Petrochemical UniversityFushunLiaoning113001PR China Industrial Mathematics Laboratory Baikal School of BRICSIrkutsk National Research Technical UniversityIrkutsk664074Russia Department of Ultrasound General Hospital of Central Theater Command of People's Liberation ArmyWuhan430070PR China Liaoning Provincial Key Laboratory of Novel Micro-nano Functional Materials Liaoning Petrochemical UniversityFushunLiaoning113001PR China

It is difficult to use a single edge operator in image processing to extract continuous and accurate contours of a porous bioelastomer due to the fuzzy boundary and complex background in ultrasound *** solve this problem,this paper proposes a joint algorithm for bioelastomer contour detection and a texture feature extraction method for monitoring the degradation performance of ***,the mean-shift clustering method is utilized to obtain the clustering feature information of bioelastomers and native tissue from manually segmented images,and this information is used as the initial information in the image binarization algorithm for image ***,Otsu's thresholding method and mathematical morphology are applied in the process of image ***,the Canny edge detector is employed to extract the complete bioelastomers contour from the binary *** verify the robustness of the proposed joint algorithm,the results using the proposed joint algorithm,where mean-shift clustering is replaced with k-means clustering are also *** proposed joint algorithm based on mean-shift clustering outperforms the joint algorithm based on k-means clustering,as well as algorithms that directly apply the Canny,Sobel and Laplacian *** feature extraction is based on the computer-aided recognition of *** region of interest(ROI)is set in the scaffold region,and the first-order statistical features and second-order statistical features of the greyscale values of the ROI are extracted and *** proposed joint algorithm can not only extract ideal bioelastomers contours from ultrasound images but also provide valuable feedback on the degradation behaviour of bioelastomers at implant sites.

关键词： Ultrasound imaging Computer-aided recognition Tissue repair Bioelastomers

Pulmonary Metastasis of a Gigantic Cystosarcoma Phyllodes of the Breast

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Chinese Medical Journal 2018年第6期131卷 751-752页

作者： yan-hong gao Hui-Juan Xiang Jian Zhu Yun Duan Pei Zhou Department of Ultrasound Wuhan General Hospital of the People's Liberation Army Wuhan Hubei 430070 China Department of Thoracic Cardiovascular Surgery Wuhan General Hospital of the People's Liberation Army Wuhan Hubei 430070 China

To the Editor： Phyllodes tumors are rare fibroepithelial malignancies of the breast and account for 〈1% of all malignant breast tumors. It is difficult to distinguish from fibroadenomas, while malignant cystosarcoma pbyllodes can grow in size quickly and metastasize early. Gigantic cystosarcoma phyllodes of the breast is very rare, thus limiting our understanding of the disease and influencing diagnostic and management outcomes. Here, we reported a case of pulmonary metastasis of a gigantic cystosarcoma phyllodes of the breast with a maximum diameter of nearly 15 era.

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