检索结果-南通市图书馆

Selection of Reference Genes for Expression Analysis of Kumamoto and Portuguese Oysters and Their Hybrid

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2017年第6期16卷 1139-1147页

作者： yan Lulu SU Jiaqi WANG Zhaoping yan xiwu YU Ruihai Fisheries College Ocean University of China South China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Engineering Research Center of Shellfish Culture and Breeding of Liaoning Province College of Fisheries and Life ScienceDalian Ocean University

Quantitative real-time polymerase chain reaction(q RT-PCR) is a rapid and reliable technique which has been widely used to quantifying gene transcripts(expression analysis). It is also employed for studying heterosis, hybridization breeding and hybrid tolerability of oysters, an ecologically and economically important taxonomic group. For these studies, selection of a suitable set of housekeeping genes as references is crucial for correct interpretation of q RT-PCR data. To identify suitable reference genes for oysters during low temperature and low salinity stresses, we analyzed twelve genes from the gill tissue of Crassostrea sikamea(SS), Crassostrea angulata(AA) and their hybrid(SA), which included three ribosomal genes, 28 S ribosomal protein S5(RPS5), ribosomal protein L35(RPL35), and 60 S ribosomal protein L29(RPL29); three structural genes, tubulin gamma(TUBγ), annexin A6 and A7(AA6 and AA7); three metabolic pathway genes, ornithine decarboxylase(OD), glyceraldehyde-3-phosphate dehydrogenase(GAPDH) and glutathione S-transferase P1(GSP); two transcription factors, elongation factor 1 alpha and beta(EF1α and EF1β); and one protein synthesis gene(ubiquitin(UBQ). Primers specific for these genes were successfully developed for the three groups of oysters. Three different algorithms, ge Norm, Norm Finder and Best Keeper, were used to evaluate the expression stability of these candidate genes. Best Keeper program was found to be the most reliable. Based on our analysis, we found that the expression of RPL35 and EF1α was stable under low salinity stress, and the expression of OD, GAPDH and EF1α was stable under low temperature stress in hybrid(SA) oyster; the expression of RPS5 and GAPDH was stable under low salinity stress, and the expression of RPS5, UBQ, GAPDH was stable under low temperature stress in SS oyster; the expression of RPS5, GAPDH, EF1β and AA7 was stable under low salinity stress, and the expression of RPL35, EF1α, GAPDH and EF1β was stable under low

关键词： Crassostrea sikamea Crassostrea angulata hybrid oyster reference gene quantitative real-time PCR

Impact of seawater acidification on shell property of the Manila clam Ruditapes philippinarum grown within and without sediment

在线全文

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 2020年第1期38卷 236-248页

作者： YUAN Hongmei XU Xian yanG Feng ZHAO Liqiang yan xiwu Engineering Research Center of Shellfish Culture and Breeding of Liaoning Province Dalian Ocean UniversityDalian 116023China Ecological Monitoring and Evaluation Division Wuhan SINOECO Ecological Science&Technology Co.Ltd.Wuhan 430080China

Although the impact of ocean acidification on marine bivalves has been previously investigated under mainly controlled laboratory conditions,it is still unclear whether the impact of acidification on sediment-burrowing species differs between those within or without *** order to fill this gap in our knowledge,we compared shell properties of the infaunal Manila clam(Ruditapes philippinarum)exposed to three pH concentrations(7.4,7.7,and 8.0),within and without *** the first experiment(140 d),clams were exposed to seawater in an acidification system without sediment.A decrease in shell weight corresponding to the increase in dissolution rate was observed in the group ofpH 7.4,at which shell color disappeared or *** observations confirmed the changes of the external shell *** the second experiment(170 d),sediment was placed at the bottom of each exposure *** effects were found obvious in shell dissolution rate and shell color in the shell specimens exposed to overlying seawater but not found in the shell specimens exposed to *** the experimental period was longer in the second experiment,shell specimens in the first experiment were more seriously damaged than those in the second experiment under acidic seawater *** results,in relation to the defense function of the shell,show that marine bivalves in burrowing behavior are more adaptable to seawater acidification than those who do not burrow into sediment.

关键词： ocean acidification sediment Manila clam(Ruditapes philippinarum) shell properties

Mass Selection for Fast Growth in the Third Generation of the Orange Line of the Manila Clam Ruditapes philippinarum

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2019年第6期18卷 1481-1485页

作者： LIANG Jian HUO Zhongming GUO Yongjun LI Yongren yan xiwu Engineering Research Center for Shellfish Culture and Breeding of Liaoning Province College of Fisheries and Life ScienceDalian Ocean UniversityDalian 116023China Tianjin Key Laboratory of Aqua-Ecology and Aquaculture Department of Fishery SciencesTianjin Agricultural UniversityTianjin 300384China Tianjin Marine Ranching Technical Engineering Center Tianjin 300457China

The Manila clam Ruditapes philippinarum is natively distributed along the Pacific coast of Asia,where it is one of the most important bivalve species for local *** species has been intentionally introduced due to its high adaptability to various coastal environments and is currently widely cultivated in Europe and North *** have initiated a selective breeding program to improve the growth rate of the Manila clam since 2007,and a full-sib family with orange shell color was *** family features high survival but slow ***,two generations of mass selection were conducted in this family by 10% upward selection for faster *** 2011,three types of lines were produced by selecting the breeder from the second-generation of selected *** lines are SS(subject to three generations of selection for growth in shell length),SC(selected for the second but not the third generation),and C(randomly sampled individuals from the national population).The genetic parameters of the shell length including hR^2(realized heritability),SR(selection response),and GG(genetic gain)were *** showed that the shell length of the SS line was significantly larger than those of the SC and C lines at all developmental stages(P<0.05).For the larval stage,the values of hR^2,SR,and GG were 0.42,0.73,and 6.66,*** the juvenile stage,the values of hR^2,SR,and GG were 0.40,0.69,21.76,*** grow-out stage,the values of hR^2,SR,and GG were 0.48,0.83,and 18.22,*** results of hR^2 indicated the good potential of the SS line in selective *** level of GG improvement was encouraging and consistent with the previous *** inbreeding depressions in the shell length were observed in the SS and SC lines at all sampling *** of the results indicate that maintaining selection pressure in successive generations may be effective in the selective breeding program of this family.

关键词： Ruditapes philippinarum mass selection realized heritability genetic gain inbreeding depression

温经通络汤含药血清对小鼠软骨细胞损伤及IκB-ζ/HIF-1α/LDHA轴的影响研究

在线全文

学校读者我要写书评

暂无评论

南京中医药大学学报 2024年第5期40卷 469-478页

作者：魏伟彭晨健顾任钧颜习武叶嘉鹏黄桂成孙鲁宁南京中医药大学附属医院江苏南京210029 南京中医药大学第一临床医学院江苏南京210023 南京中医药大学附属南京中医院江苏南京210001

目的研究温经通络汤含药血清对白细胞介素-1β(IL-1β)诱导的小鼠原代软骨细胞损伤的影响及机制。方法采用IL-1β诱导小鼠原代软骨细胞损伤模型,检测细胞中炎症因子IL-1β、IL-6、TNF-α,软骨降解相关蛋白酶基质金属蛋白酶(MMP)3、MMP9... 详细信息

目的研究温经通络汤含药血清对白细胞介素-1β(IL-1β)诱导的小鼠原代软骨细胞损伤的影响及机制。方法采用IL-1β诱导小鼠原代软骨细胞损伤模型,检测细胞中炎症因子IL-1β、IL-6、TNF-α,软骨降解相关蛋白酶基质金属蛋白酶(MMP)3、MMP9、MMP13、ADAM金属肽酶含血小板反应蛋白1基元4(ADAMTS4)以及糖酵解相关酶乳酸脱氢酶A(LDHA)、M2型丙酮酸激酶(PKM2)、还原型辅酶Ⅱ氧化酶2(NOX2)、还原型辅酶Ⅱ氧化酶4(NOX4)的mRNA表达;测定细胞内MMP3、MMP13、P65、IκB-ζ、缺氧诱导因子1α(HIF-1α)以及LDHA蛋白表达水平;进一步检测细胞上清液中一氧化氮(NO)、丙二醛(MDA)和乳酸的浓度,测定细胞内辅酶Ⅰ(NAD)的还原态(NADH)和氧化态(NAD+)的比率,以及细胞内活性氧(ROS)水平。结果温经通络汤含药血清可显著抑制IL-1β软骨细胞内IL-1β、IL-6、TNF-α的mRNA表达(P<0.01),降低细胞上清液中NO浓度(P<0.05,P<0.01),下调IκB-ζ(P<0.05)和P65蛋白表达(P<0.05,P<0.01)。温经通络汤含药血清可显著下调MMP3、MMP9、MMP13以及ADAMTS4 mRNA表达(P<0.01),抑制MMP13(P<0.05,P<0.01)和MMP3(P<0.05)的蛋白表达。氧化应激方面,它可显著抑制软骨细胞内ROS的产生,提高NADH/NAD+比率,降低上清液中MDA浓度,下调HIF-1α蛋白表达(P<0.01)。温经通络汤含药血清可显著降低乳酸浓度,下调LDHA、PKM2、NOX2、NOX4的表达,降低细胞内糖酵解水平(P<0.05,P<0.01)。结论温经通络汤含药血清可通过抑制炎症、软骨降解、氧化应激和糖酵解,缓解IL-1β诱导的小鼠原代软骨细胞损伤,其机制可能与调控IκB-ζ/HIF-1α/LDHA轴有关。

关键词：温经通络汤含药血清 IκB-ζ/HIF-1α/LDHA轴白细胞介素-1β 软骨细胞损伤

Inbreeding Depression on Growth and Survival of Full-Sib Family of Manila Clam(Ruditapes philippinarum)

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2017年第1期16卷 145-150页

作者： HUO Zhongming LI Na ZHANG Xuekai LI Ying yan xiwu yanG Feng College of Fisheries and Life Science Dalian Ocean UniversityDalian 116023P.R.China Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province Dalian Ocean UniversityDalian 116023P.R.China

In present study, the inbreeding depression(ID) of growth and survival of Manila clam(Ruditapes philippinarum) was investigated at larval and juvenile stages. Nine inbred families(A_2, B_2, C_2, D_2, E_2, F_2, G_2, H_2 and I_2) were established by mating within nine full-sib families with expected inbreeding coefficient of 0.25. Inbred families showed significant differences in shell length and hatching rate of D-larvae(straight-hinged larvae). The larvae of the nine inbred families grew slower than those of control group(CG), and their ID value ranged from 0.81% ± 6.09% to 16.10% ± 1.49%. The ID value of larval survival rate varied between 27.47% ± 9.36% and 70.50% ± 13.66%. The ID was also detected for juvenile growth in A_2, B_2, C_2, and D_2, which ranged from 4.60 ± 2.21 to 17.71 ± 7.73. The A_2 family maintained the highest juvenile survival rate, whereas the other inbred families exhibited ID values varying between 62.79% ± 4.54% and 96.14% ± 0.87%. The linear relationship of estimated ID between growth and survival was negatively correlated(R =-0.434, P < 0.05). The results of this study suggested that the ID of growth was common at the larval stage but was less prevalent at juvenile stage. In contrast, the ID of survival increased from larval to juvenile stage. A better understanding of the effect of inbreeding may aid to selective breeding of Manila clam.

关键词： Ruditapes philippinarum growth survival inbreeding depression

通脑益智方对非痴呆型血管性认知障碍脾肾亏虚、痰瘀阻络证患者认知功能及肠道菌群的影响

在线全文

学校读者我要写书评

暂无评论

中医杂志 2024年第13期65卷 1353-1362页

作者：颜习武亢姣阳孟婷刘金凤来迎龙祝怡薇常诚南京中医药大学附属医院江苏省南京市210029 南京中医药大学第一临床医学院

目的探讨通脑益智方治疗非痴呆型血管性认知障碍(VCIND)脾肾亏虚、痰瘀阻络证患者的作用机制。方法92例VCIND脾肾亏虚、痰瘀阻络证患者随机分为治疗组50例和对照组42例。两组均予常规基础治疗,对照组联合口服多奈哌齐片,每次5 mg,每晚1... 详细信息

目的探讨通脑益智方治疗非痴呆型血管性认知障碍(VCIND)脾肾亏虚、痰瘀阻络证患者的作用机制。方法92例VCIND脾肾亏虚、痰瘀阻络证患者随机分为治疗组50例和对照组42例。两组均予常规基础治疗,对照组联合口服多奈哌齐片,每次5 mg,每晚1次;治疗组联合通脑益智方,每日1剂,水煎分早晚两次口服。两组均连续治疗3个月。治疗前后比较两组患者简易精神状态检查量表(MMSE)评分、血管性痴呆评估量表(VaDAS-Cog)评分、日常生活能力量表(ADL)评分、中医证候评分(主症包括思维呆滞、善忘无记、性情改变、语言错乱,次症包括腰膝酸软、头晕头痛、耳鸣时作、神疲乏力、肢体困重、失眠烦躁、纳呆脘胀、面部发麻)。比较两组患者肠道菌群多样性和菌群丰度结构的变化及粪便中短链脂肪酸(SCFAs)水平(包括乙酸、丙酸、丁酸、异丁酸、异戊酸、戊酸、己酸)。并纳入同期20例健康受试者粪便相关数据作为参照。研究过程中进行安全性观察。结果与本组治疗前比较,两组患者治疗后MMSE评分升高,VaDAS-cog评分降低(P<0.05或P<0.01),治疗组ADL评分较治疗前升高(P<0.05)。两组患者治疗后思维呆滞、善忘无记、性情改变、语言错乱、肢体困重、失眠烦躁、纳呆脘胀、面部发麻评分及中医证候总积分均较治疗前明显降低(P<0.05或P<0.01);治疗后组间比较,治疗组腰膝酸软、耳鸣时作、神疲乏力、肢体困重、失眠烦躁、纳呆脘胀评分较对照组明显下降(P<0.05或P<0.01)。肠道菌群多样性结果显示,治疗组Shannon指数较治疗前升高(P<0.05)。治疗后各样本在主坐标分析图上经Anosim分析比较差异有统计学意义(P<0.01),提示两组间物种发生改变。治疗后,治疗组拟杆菌门和梭杆菌门相对丰度上升,放线菌门、疣微菌门和蓝藻菌门等相对丰度下降(P<0.05),普拉梭菌属、双歧杆菌属、乳酸杆菌属、瘤胃球菌属相对丰度明显升高(P<0.05)。两组患者治疗后与健康人群肠道菌群物种多样性比较,治疗组人群的肠道菌群结构向健康受试者靠近,而对照组无此趋势。治疗组治疗后乙酸、丙酸、丁酸均较治疗前升高(P<0.05或P<0.01)。结论通脑益智方可改善VCIND脾肾亏虚、痰瘀阻络证患者的认知能力和生活质量,调节肠道微生态可能是其作用机制之一。

关键词：非痴呆型血管性认知障碍脾肾亏虚痰瘀阻络认知功能肠道菌群短链脂肪酸通脑益智方

Larval and Juvenile Growth Performance of Manila Clam Hybrids of Two Full-Sib Families

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2015年第3期14卷 564-568页

作者： HUO Zhongming yan xiwu ZHAO Liqiang LIANG Jian yanG Feng ZHANG Guofan College of Fisheries and Life Science Dalian Ocean University Key Laboratory of Experimental Marine Biology Institute of Oceanography Chinese Academy of Science

In order to determine whether growth performance could be improved by hybridizing full-sib families of Manila clam （Ruditapes philippinarum）, crosses between two full-sib families including self and reciprocal crosses were carried out. The effects of heterosis, combining ability and interaction on the growth of shell length were estimated. The results showed that the growth of hybrid larvae was intermediate between parents on days 6 and 9. Heterosis on shell length was observed, which varied at juvenile stage. The cross of ♂A×♀B （Hp varied between 10.41% and 68.27%） displayed larger heterosis than ♂A×♀B （Hp varied between 1.89% and 32.33%） did, suggesting that ♂A×♀B was an ideal hatchery method of improving the growth performance of Manila clam. The variances of general combining ability （GCA）, special combining ability （SCA） and interaction （I） were significant in shell length （P〈 0.05）, indicating that both additive and non-additive genetic factors were important contributors to the growth of larvae and juveniles. The GCA for shell length of ♂A×♀B was higher than that of ♂A×♀B at both larval and juvenile stages. This con- firmed that the cross between ♂A and ♀B showed great growth in shell length. In summary, the growth of Manila clam seeds could be improved by hybridizing selected parents from large numbers of full-sib families.

关键词： Manila clam Ruditapes philippinarum growth hybrids family

Early Embryo and Larval Development of Inviable Intergeneric Hybrids Derived from Crassostrea angulata and Saccostrea cucullata

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2016年第3期15卷 515-522页

作者： SU Jiaqi WANG Zhaoping ZHANG Yuehuan yan xiwu LI Qiongzhen YU Ruihai Key Laboratory of Mariculture of Ministry of Education Fisheries College Ocean University of China Key Laboratory of Tropical Marine Biological Resources and Ecology South China Sea Institute of OceanologyChinese Academy of Sciences Engineering Research Center of Shellfish Culture and Breeding of Liaoning Province College of Fisheries and Life Science Dalian Ocean University Key Laboratory of Mariculture Guangxi Academy of Fishery Science

To detect the intergeneric hybridization between the oyster Crassostrea angulata and Saccostrea cucullata coexisting along the southern coast of China, reciprocal crosses were conducted between the two species. Barriers for sperm recognizing, binding, penetrating the egg, and forming the pronucleus were detected by fluorescence staining. From the results, although fertilization success was observed in hybrid crosses, the overall fertilization rate was lower than that of intraspecific crosses. A large number of hybrid larvae died at 6–8 d after hatching, and those survived could not complete metamorphosis. C. angulata ♀× S. cucullata ♂ larvae had a growth rate similar to that of the maternal species, whereas S. cucullata ♀× C. angulata ♂ larvae grew the slowest among all crosses. Molecular genetics analysis revealed that hybrid progeny were amphimixis hybrids. This study demonstrated that hybrid embryos generated by crossing C. angulata and S. cucullata could develop normally to the larval state, but could not complete metamorphosis and then develop to the spat stage. Thus, there is a post-reproductive isolation between C. angulata and S. cucullata.

关键词： Crassostrea angulata Saccostrea cucullata intergeneric hybridization hybrid inviability reproductive isolation

Interspecific Hybridization Between Crassostrea angulata and C. ariakensis

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2015年第4期14卷 710-716页

作者： YAO Tuo ZHANG Yuehuan yan xiwu WANG Zhaoping LI Dongchun SU Jiaqi YU Ruihai Fisheries College Ocean University of China Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of Oceanology Chinese Academy of Sciences Engineering Research Center of Shellfish Culture and Breeding of Liaoning Province College of Fisheries and Life Science Dalian Ocean University

lnterspecific hybridization can generate heterosis, which is proven to be a useful tool in selective breeding programs for oyster culture. Crassostrea angulata and C. ariakensis are two important economic shellfish species in China. We conducted 2 × 2 reciprocal crosses to determine whether these two species can cross-fertilize and their hybrids can hatch, survive and perform hetero- sis. Fertilization was found symmetrical without delay. The rate of fertilization success of C. angulata ♀× C. ariakensis ♂ was lower than that of C. ariakensis ♀ x C. angulata ♂, and the success rate of both hybridizations was lower than that of two intraspecific crosses each. During the planktonic period, survival rate of the progeny was lower in the hybrid crosses than in the intraspecific crosses. On day 360, mean shell height of the progeny of C. angulata ♀ x C. angulata ♂ was highest, which was followed by that of C. angulata ♀ × C. ariakensis ♂, C. ariakensis ♀x C. ariakensis ♂ and C. ariakensis ♀× C. angulata ♂ in a descending order. Mor- phology of adults produced by the hybrid crosses was similar to that of C. angulata. Both hybrids underwent normal gonad devel- opment and produced mature gametes in the mating season. This study provided new insights into the quantitative traits in interspeci- fic crosses of Crassostrea species, thus being of guidance value for selective breeding of oyster.

关键词： Crassostrea angulata Crassostrea ariakensis interspecific hybridization gonad development heterosis