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电感耦合等离子体原子发射光谱法同时测定石灰石和白云石中镁等6种元素含量

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检验检疫学刊 2013年第6期23卷 4-7,76页

作者：陈金凤钟坚海林亚妹刘明健龙岩出入境检验检疫局福建龙岩364000 国家矿产品检测重点实验室

建立了一种同时测定石灰石和白云石中镁、硅、铝、铁、锰、磷含量的电感耦合等离子体原子发射光谱法。试样以偏硼酸锂熔融,稀硝酸浸取,外标法定量。通过优化分析条件,得到镁、硅、铝、铁、锰、磷的检测限分别为0.071μg/mL、0.23μg/mL... 详细信息

建立了一种同时测定石灰石和白云石中镁、硅、铝、铁、锰、磷含量的电感耦合等离子体原子发射光谱法。试样以偏硼酸锂熔融,稀硝酸浸取,外标法定量。通过优化分析条件,得到镁、硅、铝、铁、锰、磷的检测限分别为0.071μg/mL、0.23μg/mL、0.23μg/mL、0.061μg/mL、0.005 2μg/mL和0.015μg/mL。该法用于实际样品的测定,所得结果与标准样品的参考值相符,重复性与精密度良好,能够实现石灰石和白云石样品中多组分同时快速测定。

关键词：电感耦合等离子体原子发射光谱法石灰石白云石偏硼酸锂元素含量

胶囊中β-烟酰胺单核苷酸和烟酰胺腺嘌呤二核苷酸的测定

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食品工业 2024年第6期45卷 324-328页

作者：李雅玫李霞虹林伟坚康海宁万俊凤吴凤琪深圳海关食品检验检疫技术中心深圳518000 北京大学深圳医院深圳518000

建立高效液相色谱法同时测定胶囊中β-烟酰胺单核苷酸(β-nicotinamide mononucleotide,NMN)和烟酰胺腺嘌呤二核苷酸(nicotinamide adenine dinucleotide,NAD+)含量的方法。样品经磷酸盐缓冲溶液(PBS)提取,采用ZORBAX SB-Aq(粒径5μm,4.6 mm×250 mm)进行分离,采用0.1%甲酸水和甲醇作为流动相进行梯度洗脱,紫外检测器测定,外标法定量。在最优条件下,NMN和NAD+在1.00~100 mg/L范围内线性关系良好,相关系数分别为0.9999和0.9999。NMN和NAD+的检出限均为0.1 g/kg,定量限均为0.3 g/kg,在0.3,0.6和3.0 g/kg的加标水平下,NMN和NAD+的回收率为81.6%~101%,相对标准偏差为0.737%~2.15%。该方法灵敏度高、选择性好、准确可靠、操作简单,可用于胶囊中NMN和NAD+含量的同时快速检测。

关键词：胶囊 β-烟酰胺单核苷酸烟酰胺腺嘌呤二核苷酸高效液相色谱法

高频燃烧红外吸收法测定石灰石、白云石中碳和硫含量

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检验检疫学刊 2014年第1期24卷 11-15页

作者：陈金凤钟坚海刘明健林亚妹龙岩出入境检验检疫局福建龙岩364000 国家矿产品检测重点实验室

建立了高频燃烧红外吸收法测定石灰石和白云石中碳硫含量的方法。对称样量、助熔剂及加入量等参数进行优化。将方法用于实际样品的测定,所得结果与标准样品的参考值相符。运用统计学方法,对协同实验数据进行分析,验证了方法的准确性和... 详细信息

建立了高频燃烧红外吸收法测定石灰石和白云石中碳硫含量的方法。对称样量、助熔剂及加入量等参数进行优化。将方法用于实际样品的测定,所得结果与标准样品的参考值相符。运用统计学方法,对协同实验数据进行分析,验证了方法的准确性和可靠性。

关键词：高频燃烧红外吸收法石灰石白云石碳硫

Multi-omic characterization of genome-wide abnormal DNA methylation reveals diagnostic and prognostic markers for esophageal squamous-cell carcinoma

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Signal Transduction and Targeted Therapy 2022年第3期7卷 896-908页

作者： Yiyi Xi Yuan lin Wenjia Guo Xinyu Wang Hengqiang Zhao Chuanwang Miao Weiling Liu Yachen Liu Tianyuan Liu Yingying Luo Wenyi Fan Ai lin yamei Chen Yanxia Sun Yulin Ma Xiangjie Niu Ce Zhong Wen Tan Meng Zhou Jianzhong Su Chen Wu Dongxin lin Department of Etiology and Carcinogenesis National Cancer Center/National Clinical Research Center/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Beijing Advanced Innovation Center for Genomics Biomedical Pioneering Innovation CenterPeking UniversityBeijing 100871China Cancer Institute Affiliated Cancer Hospital of Xinjiang Medical UniversityUrumqi 830000China School of Biomedical Engineering School of Ophthalmology and Optometry and Eye HospitalWenzhou Medical UniversityWenzhou 325011China Wenzhou Institute University of Chinese Academy of SciencesWenzhou 325000China Jiangsu Collaborative Innovation Center for Cancer Personalized Medicine Nanjing Medical UniversityNanjing 211166China CAMS Key Laboratory of Genetics and Genomic Biology Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Sun Yat-sen University Cancer Center State Key Laboratory of Oncology in South ChinaGuangzhou 510060China

This study investigates aberrant DNA methylations as potential diagnosis and prognosis markers for esophageal squamous-cell carcinoma(ESCC),which if diagnosed at advanced stages has<30%five-year survival *** genome-wide methylation sites of 91 ESCC and matched adjacent normal tissues,we identified 35,577 differentially methylated CpG sites(DMCs)and characterized their distribution patterns.

关键词： esophageal squamous diagnosis

新方颠倒散体外抑制痤疮丙酸杆菌实验研究及皮肤毒性测试

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亚太传统医药 2024年第2期20卷 15-18页

作者：张瑞雪高芳芳袁亚美张彩云方正清朱林王茜安徽中医药大学安徽合肥230012

目的:研究不同配比新方颠倒散水提物溶液对痤疮丙酸杆菌体外的抑菌作用。方法:配制不同组分新方颠倒散水提物溶液A、C、E、G、I、K,颠倒散水提物溶液(M)为阳性对照,另设阴性对照、空白对照;采用37℃厌氧培养法对痤疮丙酸杆菌进行培养,... 详细信息

目的:研究不同配比新方颠倒散水提物溶液对痤疮丙酸杆菌体外的抑菌作用。方法:配制不同组分新方颠倒散水提物溶液A、C、E、G、I、K,颠倒散水提物溶液(M)为阳性对照,另设阴性对照、空白对照;采用37℃厌氧培养法对痤疮丙酸杆菌进行培养,观察各溶液体外的抑菌效果;并采用单次限量法,取A进行大鼠急性经皮毒性试验。结果:培养48 h后,空白对照无菌落,其余均见菌落,各新方颠倒散水提物溶液分别与阴性对照比较,均有显著统计学意义(P0.05)。大鼠急性经皮毒性试验:A组、对照组、M组反应平均值分别为0、0、1.2,生理情况正常。结论:不同配比新方颠倒散水提物溶液体外对痤疮丙酸杆菌均有抑制作用,相互比较以及与颠倒散比较均无显著差异,且新方颠倒散溶液无皮肤刺激及中毒反应。

关键词：颠倒散新方颠倒散痤疮丙酸杆菌体外抑菌实验皮肤毒性试验

小型化宽阻带LTCC微波低通滤波器的设计与研制

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电子元件与材料 2016年第8期35卷 72-75,85页

作者：罗洪梁林亚梅朱建华刘季超胡志明深圳振华富电子有限公司广东深圳518109

先根据滤波器衰减量要求,理论分析确定滤波器的阶数及电路原理图,再用ADS软件优化仿真获得电路元件初始值,然后采用微波电磁场仿真软件 HFSS 对滤波器物理模型优化仿真,成功设计了-款 3 dB 截止频率为 1 750 MHz、宽阻带频率2 035~6... 详细信息

先根据滤波器衰减量要求,理论分析确定滤波器的阶数及电路原理图,再用ADS软件优化仿真获得电路元件初始值,然后采用微波电磁场仿真软件 HFSS 对滤波器物理模型优化仿真,成功设计了-款 3 dB 截止频率为 1 750 MHz、宽阻带频率2 035~6 700 MHz范围内衰减量大于20 dB的LTCC微波低通滤波器,并采用LTCC工艺制备该微波低通滤波器,尺寸为3.2 mm×1. 6 mm×0.9 mm.制备样品实测结果与仿真吻合,说明采用该方法设计、研制小型化宽阻带LTCC微波低通滤波器是可行的.

关键词： LTCC 微波低通滤波器小型化电磁场仿真 HFSS ADS

Highly active bifunctional catalyst: Constructing FeWO_(4)-WO_(3) heterostructure for water and hydrazine oxidation at large current density

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Nano Research 2021年第11期14卷 4356-4361页

作者： Fang Shen Zhenglin Wang yamei Wang Guangfu Qian Miaojing Pan lin Luo Guoning Chen Hailang Wei Shibin Yin College of Chemistry and Chemical Engineering Key Laboratory of New Processing Technology for Nonferrous Metals and MaterialsState Key Laboratory of Processing for Non-Ferrous Metal and Featured MaterialsGuangxi UniversityNanning530004China Guangxi Bossco Environmental Protection Technology Co. Ltd12 Kexing RoadNanning530007China

Developing high performance anode catalysts for oxygen evolution reaction (OER) and hydrazine oxidation reaction (HzOR) at large current density is an efficient pathway to produce hydrogen. Herein, we synthesize a FeWO_(4)-WO_(3) heterostructure catalyst growing on nickel foam (FeWO_(4)-WO_(3)/NF) by a combination of hydrothermal and calcination method. It shows good catalytic activity with ultralow potentials for OER (ζ_(10) = 1.43 V, ζ_(1.000) = 1.56 V) and HzOR (ζ_(10) = −0.034 V, ζ_(1.000) = 0.164 V). Moreover, there is little performance degradation after being tested for _(10)0 h at 1,000 (OER) and _(10)0 (HzOR) mA·cm−2, indicating good stability. The superior performance could be attributed to the wolframite structure and heterostructure: The former provides a high electrical conductivity to ensure the electronic transfer capability, and the later induces interfacial electron redistribution to enhance the intrinsic activity and stability. The work offers a brand-new way to prepare good performance catalysts for OER and HzOR, especially at large current density.

关键词： catalyst heterostructure large current density oxygen evolution reaction hydrazine oxidation reaction

Verification of Mutagen Function of Zeocin in Nannochloropsis oceanica Through Transcriptome Analysis

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Journal of Ocean University of China 2017年第3期16卷 501-508页

作者： lin Genmei WANG yamei GUO Li DING Haiyan HU Yongmei LIANG Sijie ZHANG Zhongyi YANG Guanpin Laboratory of Marine Genetics and Breeding Ocean University of ChinaQingdao 266003P.R.China College of Marine Life Sciences Ocean University of ChinaQingdao 266003P.R.China Institutes of Evolution and Marine Biodiversity Ocean University of ChinaQingdao 266003P.R.China

Zeocin can cause double strand breaks of DNA and thus is frequently used as a selective antibiotic of eukaryotic Sh ble transformants. In non-transformation system, Zeocin may function as a mutagen if not totally lethal. To verify such function of Zeocin, we mutated Nannochloropsis oceanica by increasing the concentration of Zeocin in medium gradually, and isolated a N. oceanica strain(single cell culture) which survived Zeocin up to 10.0μg mL^(-1). The Zeocin-tolerant strain entered the exponential growth phase later and grew slower than the wild strain. Transcriptome profiling showed that the Zeocin-tolerant N. oceanica strain survived Zeocin mainly by adapting(heritable), rather than acclimating(plastic) to Zeocin. Hence mutating N. oceanica with Zeocin was approved effective. Meanwhile, the physiological characteristics of this Zeocin-tolerant strain were demonstrated. As we proposed, N. oceanica tolerated Zeocin by strengthening its protein degradation and antioxidation. The genes controlling cell division and cellular response to stimuli may also have played important roles in the reduction of growth and the tolerance to Zeocin. Our findings evidenced that Zeocin can serve as an appropriate mutagen of microalgae. Creating variations through mutation with Zeocin may help to study the genetic basis of the traits of this monoploidy and asexual microalga, as well as improve its production.

关键词： Nannochloropsis 大洋洲变化 Zeocin 忍耐 transcriptome

无创方法诊断HBeAg阳性慢性乙型肝炎患者肝内炎症反应程度

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检验医学与临床 2024年第2期21卷 237-241,246页

作者：叶雅妹周丽娜杨文彦孙芳林勇林春潘晨福建医科大学孟超肝胆医院代谢性肝病科福建福州350000

目的建立由简单常规实验数据组成的模型,以预测HBeAg阳性且丙氨酸氨基转移酶(ALT)≤2倍正常上限的慢性乙型肝炎(CHB)患者的肝脏炎症反应程度。方法选取2019年4月1日至2021年5月8日151例HBeAg阳性且ALT≤2倍正常上限的CHB患者作为研究对... 详细信息

目的建立由简单常规实验数据组成的模型,以预测HBeAg阳性且丙氨酸氨基转移酶(ALT)≤2倍正常上限的慢性乙型肝炎(CHB)患者的肝脏炎症反应程度。方法选取2019年4月1日至2021年5月8日151例HBeAg阳性且ALT≤2倍正常上限的CHB患者作为研究对象,根据肝脏病理检查结果分为轻度炎症反应[肝组织炎症分级(G)G0~1]组和中度炎症反应(G≥2)组,采用多因素Logistic回归分析CHB患者发生中度炎症反应的独立危险因素并建立诊断模型。结果53.0%(80/151例)的研究对象出现G≥2。G≥2组ALT、天门冬氨酸氨基转移酶(AST)水平均明显高于G0~1组,胆碱酯酶(ChE)、前蛋白比(PA)、HBsAg均明显低于G0~1组,差异均有统计学意义(P<0.05)。ALT、AST与G分级均呈弱正相关(r=0.212、0.303,P<0.05),ChE、PA、HBsAg与G分级均呈弱负相关(r=-0.371、-0.330、-0.173,P<0.05)。高水平AST、低水平PA、低水平logChE为CHB患者发生中度炎症反应的独立危险因素(P<0.05)。模型CPA对预测发生G≥2的受试者工作特征曲线下面积为0.842(95%CI:0.741~0.935)。当CPA≤0.3,其排除G≥2的阴性预测值为84.21%;当CPA≥0.6时,CPA诊断发生G≥2的阳性预测值为84.00%。结论AST、PA和logChE与HBsAg阳性且ALT≤2倍正常上限的CHB患者的肝内炎症反应程度相关。CPA对发生G≥2具有较好的诊断效能,且该模型涉及的指标简单易得,适合临床推广使用。

关键词：慢性乙型肝炎乙型肝炎e抗原肝穿刺炎症反应无创模型