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Feasibility of terahertz spectroscopy for hybrid purity verification of rice seeds

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International Journal of Agricultural and Biological Engineering 2018年第5期11卷 65-69页

作者： yaling yang Shengling Zhou Jie Song Jie Huang Guanglin Li Shiping Zhu College of Physical Science and Technology Southwest UniversityChongqing 400715China College of Engineering and Technology Southwest UniversityChongqing 400715China

The purity of hybrid rice seeds reflects the typical consistency of seed varieties in characteristics.The accuracy and reliability of seed purity detecting are of great significance to ensure the quality of seeds.In this study,the feasibility of identifying the purity of hybrid rice seeds,Xinong 1A/89,by terahertz(THz)time-domain spectroscopy system combined with chemometrics was explored.Three quantitative identification models for testing the purity of Xinong 1A/89 hybrid rice seed were developed and compared by THz absorption spectroscopy with extreme learning algorithm(ELM),Principal cComponent Regression(PCR)and Partial Least Squares Regression(PLSR).Experimental results showed that comparing with classical PLSR and PCR models,ELM presents a better feasibility and stability.For the testing set,the quantitative prediction result of ELM(ELoo=2.005×10^(-5),R^(2)=96.75%)is significantly better than those of PCR(ELoo=7.346×10^(-5),R^(2)=88.10%)and PLSR(ELoo=8.007×10^(-5),R^(2)=87.03%).The results highlight the feasibility of THz spectroscopy combined with ELM as an efficient and reliable method for verification of hybrid rice seeds.

关键词： purity detection hybrid rice seeds terahertz spectroscopy extreme learning algorithm

基于Hg/Au基纳米酶过氧化物酶活性比色/SERS双模式检测烟碱

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分析试验室 2024年第4期43卷 488-496页

作者：张海何彬高宪辉周渭皓刘磊李秋兰吴伟杨德志杨亚玲云南省烟草烟叶公司昆明650218 昆明理工大学生命科学与技术学院昆明650500

利用Hg^(2+)与Au基纳米材料形成金汞齐制备Hg/Au基纳米酶(AuNPs@Hg)。基于纳米酶表面增强拉曼光谱(SERS)活性和模拟酶催化活性,建立了比色/SERS双模式检测烟碱的方法。结果表明,AuNPs@Hg可以模拟过氧化物酶,催化过氧化氢氧化无色的3,3’... 详细信息

利用Hg^(2+)与Au基纳米材料形成金汞齐制备Hg/Au基纳米酶(AuNPs@Hg)。基于纳米酶表面增强拉曼光谱(SERS)活性和模拟酶催化活性,建立了比色/SERS双模式检测烟碱的方法。结果表明,AuNPs@Hg可以模拟过氧化物酶,催化过氧化氢氧化无色的3,3’,5,5’-四甲基联苯胺(TMB),同时也可以作为SERS底物,检测TMB氧化产物(oxTMB)的SERS信号,而烟碱则可以显著增加AuNPs@Hg的过氧化物酶活性。基于比色法,在烟碱浓度1.25~375μg/mL范围内呈良好的线性关系,检出限(LOD)为0.34μg/mL。基于SERS法,烟碱浓度在6.25×10^(-3)~1.25 mg/mL范围内有较好的线性校准曲线,LOD为1.80μg/mL。实际样品中烟碱的回收率在96.1%~105.3%范围内,相对标准偏差(RSD)为2.4%~4.7%,且实际样品中存在的共存物质对所建立的方法几乎无干扰作用,检测结果与行业标准方法测定结果一致。本研究可为多功能传感器的设计及SERS在烟草行业中的应用提供新的策略和途径。

关键词：烟碱纳米酶表面增强拉曼光谱过氧化物酶金汞齐

孕康口服液结合黄体酮治疗习惯性流产患者的效果及对E2水平的影响

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临床医学研究与实践 2024年第17期9卷 83-86页

作者：卫英娟杨亚玲西安新长安妇产医院陕西西安710001

目的探讨孕康口服液结合黄体酮治疗习惯性流产患者的效果及对雌二醇(E2)水平的影响。方法选取2021年9月至2022年9月进入本院治疗的100例习惯性流产患者作为研究对象,以随机数字表法将其分为对照组与观察组,各50例。对照组给予黄体酮治疗... 详细信息

目的探讨孕康口服液结合黄体酮治疗习惯性流产患者的效果及对雌二醇(E2)水平的影响。方法选取2021年9月至2022年9月进入本院治疗的100例习惯性流产患者作为研究对象,以随机数字表法将其分为对照组与观察组,各50例。对照组给予黄体酮治疗,观察组给予孕康口服液结合黄体酮治疗。比较两组的治疗效果。结果治疗前,两组的E2、孕酮(P)、人绒毛膜促性腺激素(β-HCG)及卵泡刺激素(FSH)水平比较,差异无统计学意义(P>0.05);治疗后,两组的E2、P、β-HCG及FSH水平均升高,且观察组高于对照组,差异具有统计学意义(P0.05);治疗后,两组的sHLA-G、TGF-β1及PIBF水平均升高,且观察组高于对照组,差异具有统计学意义(P<0.05)。结论孕康口服液结合黄体酮可改善习惯性流产患者的激素及免疫指标水平,促进疾病症状消退,值得推广。

关键词：孕康口服液黄体酮习惯性流产雌二醇

毛钩藤碱在脑缺血再灌注损伤动物模型中的保护作用

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立体定向和功能性神经外科杂志 2024年第1期37卷 22-27页

作者：江华徐达杨雅玲李清金联勤保障部队第九〇九医院(厦门大学附属东南医院)神经内科漳州363000

目的探究毛钩藤碱(Hirsutine)在大鼠脑缺血再灌注损伤(CIRI)中的神经保护作用及其潜在机制。方法SD大鼠用于建立大脑中动脉闭塞再灌注(MCAO/R)模型,并将大鼠分为6个实验组(n=6):对照组;模型组;Hirsutine低剂量(5 mg/kg)组;Hirsutine中剂... 详细信息

目的探究毛钩藤碱(Hirsutine)在大鼠脑缺血再灌注损伤(CIRI)中的神经保护作用及其潜在机制。方法SD大鼠用于建立大脑中动脉闭塞再灌注(MCAO/R)模型,并将大鼠分为6个实验组(n=6):对照组;模型组;Hirsutine低剂量(5 mg/kg)组;Hirsutine中剂量(10 mg/kg)组;Hirsutine高剂量(20 mg/kg)组;依达拉奉(30 mg/kg,阳性对照)组。取脑组织进行TTC染色,测量脑梗死和神经行为缺陷;HE和Nissl染色检测神经元损伤;Tunel染色检测海马细胞凋亡;ELISA法检测脑组织活性氧(ROS),丙二醛(MDA),超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)水平;蛋白质印迹检测机制相关蛋白的表达水平。结果Hirsutine治疗显著减轻了MCAO/R大鼠的脑梗死和神经元损伤,并改善了脑神经行为缺陷。Hirsutine有效抑制氧化应激和细胞凋亡。此外,Hirsutine激活了MCAO/R大鼠神经元中的PI3K/Akt/Nrf2通路。结论Hirsutine通过激活PI3K/Akt/Nrf2信号通路改善MCAO/R大鼠脑组织氧化应激和细胞凋亡,从而缓解CIRI诱导的神经元损伤。

关键词：毛钩藤碱脑缺血再灌注神经损伤氧化应激凋亡

肝细胞核因子1β突变所致的以肾囊肿为突出表现的早发糖尿病临床特征及基因突变分析

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中华糖尿病杂志 2024年第3期16卷 336-340页

作者：徐新月杨亚玲瞿朵朵吴宸炜刘佳朱琴王春红赵晓龙上海市公共卫生临床中心内分泌代谢科上海200000

目的对1例因肝细胞核因子1β(HNF1β)突变所致的以肾囊肿为突出表现的新发青少年起病的成人型糖尿病(MODY)5型患者的临床资料进行回顾性分析,并对我国已发表MODY5患者的临床特征进行总结。方法选取1例于2021年7月就诊上海市公共卫生临... 详细信息

目的对1例因肝细胞核因子1β(HNF1β)突变所致的以肾囊肿为突出表现的新发青少年起病的成人型糖尿病(MODY)5型患者的临床资料进行回顾性分析,并对我国已发表MODY5患者的临床特征进行总结。方法选取1例于2021年7月就诊上海市公共卫生临床中心内分泌代谢科的MODY5型糖尿病患者作为研究对象,对患者的临床资料、就诊及随访经过和基因检测结果进行详细描述,并在美国国立医学图书馆数据库(PubMed)、中国期刊全文数据库(CNKI)和万方数据库自建库之日起至2022年8月15日检索所有关于中国MODY5患者的病例报告,综合分析检索所得患者的相关临床数据。以基因突变类型将患者分为杂合突变体和缺失突变2类,采用Mann‐Whitney U检验对2类患者间相关指标进行比较。结果上海市公共卫生临床中心内分泌代谢科MODY5患者19岁糖尿病起病,糖尿病自身免疫抗体均阴性,合并肾囊肿、高尿酸、肾脏发育不良、胰腺萎缩,携带HNF1β基因2号内含子c.544+3_544+6del缺失突变,该突变基因来自其母亲。文献检索共纳入32例MODY5患者,包括上海市公共卫生临床中心内分泌代谢科的此例MODY5患者,糖尿病起病多为青年[84.4%(27/32)],肾囊肿患病率高[84.4%(27/32)],常出现高尿酸血症[60.9%(14/23)]。32例患者共涉及27种基因突变类型,其中携带杂合突变体类型14种,缺失突变13种。携带杂合突变体的患者体重指数(BMI)及估算的肾小球滤过率(eGFR)均低于缺失突变患者[BMI分别为14.57(11.80,32.79)和16.97(13.85,22.30)kg/m^(2),eGFR分别为43.31(15.43,129.23)和73.29(53.76,146.27)ml·min^(-1)·(1.73 m^(2))^(-1),均P<0.05]。结论对于糖尿病自身免疫抗体阴性、合并肾囊肿的早发糖尿病患者,需要考虑MODY5可能,HNF1β人群易合并较低的体重和eGFR。

关键词：糖尿病青少年起病的成人型糖尿病肾囊肿高尿酸血症

鸡circMICAL2的鉴定、组织表达谱分析及其功能预测

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新疆农业科学 2024年第5期61卷 1284-1291页

作者：古丽帕日·艾克拜沈雪梅喻世刚王钢杨雅玲刘武军新疆农业大学动物科学学院乌鲁木齐830052 乐山师范学院/川南地方品种鸡产业化四川省高等学校工程研究中心四川乐山614000

【目的】验证鸡环状RNA(circMICAL2)的真实性,研究circMICAL2在鸡肌肉生长发育中的潜在功能及调控机制。【方法】基于circMICAL2环状序列设计特异性引物采用PCR扩增完成其真实性验证,并测定RNase R和放线菌素D处理检测circMICAL2的内源... 详细信息

【目的】验证鸡环状RNA(circMICAL2)的真实性,研究circMICAL2在鸡肌肉生长发育中的潜在功能及调控机制。【方法】基于circMICAL2环状序列设计特异性引物采用PCR扩增完成其真实性验证,并测定RNase R和放线菌素D处理检测circMICAL2的内源稳定性。采集16日龄三黄鸡(雏鸡)和180日龄三黄鸡(成年鸡)的心脏、肝脏、肺、肾脏、肠、皮肤、胸肌和腿肌等8个组织样本,采用实时荧光定量PCR(qRT-PCR)分析circMICAL2在鸡不同发育时期的组织表达图谱;运用生物信息学对circMICAL2靶向miRNA和mRNA进行预测,并开展GO功能和KEGG通路富集分析。【结果】鸡circMICAL2真实存在,其环化稳定性强;circMICAL2在成年鸡和雏鸡各组织中广泛表达,且circMICAL2均在成年鸡和雏鸡的胸肌和腿肌肌肉中表达量最高,circMICAL2在成年鸡腿肌和胸肌中的表达量均显著高于雏鸡(P<0.05),circMICAL2与鸡肌肉生长发育调控密切相关。circMICAL2可靶向gga-miR-103-3p和gga-miR-130b-3p,调控下游225个潜在靶基因。circMICAL2的靶基因主要富集于TGF-β信号通路、MAPK信号通路、细胞周期等相关信号通路。【结论】鸡circMICAL2真实存在,circMICAL2的表达对鸡不同生长发育时期肌肉的生长具有重要调控作用。

关键词：环状RNA 肌肉鸡组织表达生长发育

Engineering single-atom Mn on nitrogen-doped carbon to regulate lithium-peroxide reaction kinetics for rechargeable lithium-oxygen batteries

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Journal of Energy Chemistry 2024年第7期94卷 199-207页

作者： yaling Huang Yong Liu yang Liu Chenyang Zhang Wenzhang Li Jie Li School of Chemistry and Chemical Engineering Central South UniversityChangsha 410083HunanChina School of Minerals Processing and Bioengineering Central South UniversityChangsha 4003HunanChina

Precision engineering of catalytic sites to guide more favorable pathways for Li_(2)O_(2) nucleation and decom-position represents an enticing kinetic strategy for mitigating overpotential,enhancing discharge capac-ity,and improving recycling stability of Li-O_(2) batteries.In this work,we employ metal-organic frameworks(MOFs)derivation and ion substitution strategies to construct atomically dispersed Mn-N_(4) moieties on hierarchical porous nitrogen-doped carbon(Mn SAs-NC)with the aim of reducing the over-potential and improving the cycling stability of Li-O_(2) batteries.The porous structure provides more chan-nels for mass transfer and exposes more highly active sites for electrocatalytic reactions,thus promoting the formation and decomposition of Li_(2)O_(2).The Li-O_(2) batteries with Mn SAs-NC cathode achieve lower overpotential,higher specific capacity(14290 mA h g^(-1) at 100 mAg^(-1)),and superior cycle stability(>100 cycles at 200 mA g^(-1))compared with the Mn NPs-NC and NC.Density functional theory(DFT)cal-culations reveal that the construction of Mn-N_(4) moiety tunes the charge distribution of the pyridinic N-rich vacancy and balances the affinity of the intermediates(LiO_(2) and Li_(2)O_(2)).The initial nucleation of Li_(2)O_(2) on Mn SAs-NC favors the O_(2)-→LiO_(2)→Li_(2)O_(2) surface-adsorption pathway,which mitigates the overpoten-tials of the oxygen reduction(ORR)and oxygen evolution reaction(OER).As a result,Mn SAs-NC with Mn-N_(4) moiety effectively facilitates the Li_(2)O_(2) nucleation and enables its reversible decomposition.This work establishes a methodology for constructing carbon-based electrocatalysts with high activity and selectivity for Li-O_(2)batteries.

关键词： Single-atom Mn MOFs-oriented architecture Rechargeable Li-O_(2)battery N-doped carbon Density functional theory calculation

Water-soluble organic matter with various polarities in PM_(2.5) over Xi'an,China:Abundance,functional groups,and light absorption

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Particuology 2024年第1期84卷 281-289页

作者： Xueting yang Shasha Huang Dan Li Hongmei Xu yaling Zeng Liu yang Diwei Wang Ningning Zhang Junji Cao Zhenxing Shen Department of Environmental Science and Engineering Xi'an Jiaotong UniversityXi'an710049China School of Environmental Science and Engineering Southern University of Science and TechnologyShenzhen518055China State Key Laboratory of Loess and Quaternary Geology Institute of Earth EnvironmentChinese Academy of SciencesXi'an710049China

Humic-like substances(HULIS)are a major component of brown carbon and consequently play a major role in climate change.In this study,70 PM_(2.5) samples were collected from Xi'an in winter 2019 and summer 2020.Neutral HULIS(HULIS-n),acidic HULIS(HULIS-a),and high-polarity water-soluble organic compounds(HP-WSOC)were analyzed to determine their carbon concentrations and measure their ultraviolet-visible absorption and infrared spectra.Of the three components,HULIS-n had the highest carbon content in both winter(3.29±1.45μg m^(-3))and in summer(1.38±1.10μg m^(-3)).The semi-quantitative results for the functional groups revealed that HP-WSOC was rich in carboxylic acids and had high aromaticity in winter,whereas HULIS-n was rich in carboxylic acids in summer.Moreover,HULJS-a was richer in nitrate esters and saturated aliphatic hydrocarbons in summer than in winter.The results for specific ultraviolet absorbance(SUVA)and E_(250)/E_(365) revealed that HULIS had higher molecular weight and aromaticity in winter than in summer.HULIS-n dominated in the total light absorption of HULIS+HP-WSOC in both winter(73.08%)and summer(48.57%).Overall,the results on the carbon content,optical properties,and functional groups of WSOCs with differing polarity can improve un-derstanding of environmental and climatic effects.

关键词： Humic-like substances(HULIS) Aromaticity Fourier-transform infrared(FTIR) spectroscopy Light absorbance

Deliverability analysis of greedy routing in the spherical cap 3D sensor networks

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Science China(Information Sciences) 2013年第7期56卷 52-63页

作者： HOU MengShu yang yaling LI YuJun School of Computer Science and Engineering University of Electronic Science and Technology of China Department of Electrical and Computer Engineering Virginia Polytechnic Institute and State University

For 3D sensor networks to monitor the ocean, forest and aerosphere, etc., sensors can forward their data to the base station by greedy routing. It is critical to quantitatively greedy routing’s deliverability for evaluating network’s functionality and performance. The probability that all sensors can successfully send their data to the base station by greedy routing is usually modeled as the probability of guaranteed delivery. For a typical spherical cap 3D sensor network deployment scenario where nodes follow a homogeneous Poisson point process, the relationship between the sensor transmission radius and the probability of guaranteed delivery is studied, and a tight analytical upper bound on the sensor transmission radius to ensure the designed deliverability probability is derived in this paper. The correctness and tightness of the derived upper bound are verified by extensive simulations.

关键词： 3D sensor network greedy routing deliverability probability critical transmission radius