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Dlg1 Knockout Inhibits Microglial Activation and Alleviates Lipopolysaccharide-Induced Depression-Like Behavior in Mice

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Neuroscience Bulletin 2021年第12期37卷 1671-1682页

作者： Zhixin Peng Xiaoheng Li Jun Li Yuan dong Yuhao Gao yajin Liao Meichen Yan Zengqiang Yuan Jinbo Cheng Institute of Neuroscience Hengyang Medical CollegeUniversity of South ChinaHengyang 421001China The Brain Science Center Beijing Institute of Basic Medical Sciences27 Taiping RoadHaidian DistrictBeijing 100850China Beijing Institute for Brain Disorders Capital Medical UniversityBeijing 100069China Institutes of Brain Sciences and Disease Medical CollegeQingdao UniversityQingdao 266071China Center on Translational Neuroscience College of Life and Environmental ScienceMinzu University of ChinaBeijing 100081China

Microglia-mediated neuroinflammation is widely perceived as a contributor to numerous neurological diseases and mental disorders including *** large homolog 1(Dlg1),an adaptor protein,regulates cell polarization and the function of K?channels,which are reported to regulate the activation of ***,little is known about the role of Dlg1 in microglia and the maintenance of central nervous system *** this study,we found that Dlg1 knockdown suppressed lipopolysaccharide(LPS)-induced inflammation by downregulating the activation of nuclear factor-jB signaling and the mitogen-activated protein kinase pathway in ***,using an inducible Dlg1 microglia-specific knockout(Dlg1flox/flox;CX3CR1CreER)mouse line,we found that microglial Dlg1 knockout reduced the activation of microglia and alleviated the LPS-induced depressionlike *** summary,our results demonstrated that Dlg1 plays a critical role in microglial activation and thus provides a potential therapeutic target for the clinical treatment of depression.

关键词： Dlg1 Microglia Neuroinflammation Depression

HPLC法测定肉桂醛在肉桂油中的含量及在大鼠体内的药时曲线参数

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中国食品添加剂 2024年第9期35卷 206-213页

作者：史亚瑾董贝贝李寒冰吴宿慧李根林河南中医药大学河南省健康衰老产业工程研究中心郑州450046

使用高效液相色谱法(HPLC)测定肉桂挥发油中肉桂醛、肉桂醇、苯甲醛、肉桂酸等4种化学成分含量,采用白色封闭群(Wistar)大鼠灌胃给与肉桂醛,测定肉桂醛在大鼠体内的基本代谢参数。蒸馏法提取肉桂挥发油的得率为3.03 mL/g,肉桂挥发油中... 详细信息

使用高效液相色谱法(HPLC)测定肉桂挥发油中肉桂醛、肉桂醇、苯甲醛、肉桂酸等4种化学成分含量,采用白色封闭群(Wistar)大鼠灌胃给与肉桂醛,测定肉桂醛在大鼠体内的基本代谢参数。蒸馏法提取肉桂挥发油的得率为3.03 mL/g,肉桂挥发油中肉桂醛、苯甲醛、肉桂酸、肉桂醇平均含量分别为50.13%、0.64%、0.45%、3.29%。试验大鼠单次灌胃100、200、400 mg/kg剂量肉桂醛后,吸收速率常数Ka分别为20.187、18.708、20.437 min,表明肉桂醛能被迅速吸收;灌胃给药后血浆中肉桂醛在50 min即达峰浓度值(C_(max))=19.980、38.806、90.106 mg/L,达峰时间(T_(max))均在0.833h左右,半衰期(T_(1/2))为1.7h。表明肉桂醛进入大鼠体循环后,分布迅速,并快速代谢为肉桂酸,无明显的蓄积作用,其吸收和消除与给药剂量无关,代谢过程符合开放的二室模型。

关键词：肉桂肉桂醛代谢半衰期

Effect of curcumin extract against oxidative stress on both structure and deformation capability of red blood cell

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中肝叶联合尾状叶切除术在肝门部胆管癌中的应用

第十二届全国生物力学学术会议暨第十四届全国生物流变学学术会议

作者： Milody Noviana yajin Zhao Chen dong Wang Xiang Chongqing University

Objective The aim of this study is to explore the effect of pre-treatment of curcumin extract on erythrocyte deformable capabilities and erythrocyte band 3 (SLC4A1;EB3). Methods The association between alterations of EB3 and erythrocyte deformable capability induced by hydrogen peroxide has been investigated. The blood samples were divided among 3 groups:(i) control,(ii) pre-treatment either using curcumin extract or vitamin C and (iii) negative control group. The oxidant stress parameters and antioxidant status, erythrocyte deform-

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中华消化外科杂志 2021年第1期20卷 52-58页

作者：陈亚进周睿中山大学孙逸仙纪念医院肝胆外科广州510120

肝门部胆管癌是常见胆道恶性肿瘤,其外科治疗的核心是达到R0切除,包括切除病变胆管、受肿瘤浸润肝组织及区域淋巴结。在精准外科及多学科治疗时代,肝门部胆管癌的手术治疗应遵循可视化、可量化、可控化和多学科治疗原则。基于精准肝门... 详细信息

肝门部胆管癌是常见胆道恶性肿瘤,其外科治疗的核心是达到R0切除,包括切除病变胆管、受肿瘤浸润肝组织及区域淋巴结。在精准外科及多学科治疗时代,肝门部胆管癌的手术治疗应遵循可视化、可量化、可控化和多学科治疗原则。基于精准肝门部胆管癌董氏分型,包括Ⅰ+Ⅳ+Ⅴ+Ⅷ段、Ⅰ+Ⅴ+Ⅷ段及Ⅰ+Ⅳ段的中肝叶联合尾状叶切除术,在获得R0切除的同时可最大限度保留更多肝实质,使部分病人避免术前进行减轻黄疸治疗和分期手术。然而,在获得良好围术期效果的同时,还需随访评价病人的长期生存情况。笔者结合团队开展中肝叶联合尾状叶切除术的临床经验,探讨其在肝门部胆管癌中的应用。

关键词：胆道肿瘤肝门部中肝叶切除 R0切除肝实质保留

SARS-CoV-2 infection aggravates chronic comorbidities of cardiovascular diseases and diabetes in mice

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Animal Models and Experimental Medicine 2021年第1期4卷 2-15页

作者： Yuanwu Ma Dan Lu Linlin Bao yajin Qu Jiangning Liu Xiaolong Qi Lei Yu Xu Zhang Feifei Qi Qi Lv Yunpeng Liu Xudong Shi Caixian Sun Jing Li Jie Wang Yunlin Han Kai Gao Wei dong Ning Liu Shan Gao Jing Xue Qiang Wei Sidan Pan Hong Gao Lianfeng Zhang Chuan Qin Key Laboratory of Human Disease Comparative Medicine National Health Commission of China(NHC)Institute of Laboratory Animal SciencePeking Union Medicine CollegeChinese Academy of Medical SciencesBeijingChina Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases Institute of Laboratory Animal SciencePeking Union Medicine CollegeChinese Academy of Medical SciencesBeijingChina

Background:Cardiovascular diseases(CVDs)and diabetes mellitus(DM)are top two chronic comorbidities that increase the severity and mortality of ***,how SARS-CoV-2 alters the progression of chronic diseases remain ***:We used adenovirus to deliver h-ACE2 to lung to enable SARS-CoV-2 infection in ***-CoV-2’s impacts on pathogenesis of chronic diseases were studied through histopathological,virologic and molecular biology ***:Pre-existing CVDs resulted in viral invasion,ROS elevation and activation of apoptosis pathways contribute myocardial injury during SARS-CoV-2 *** infection increased fasting blood glucose and reduced insulin response in DM *** mineral density decreased shortly after infection,which associated with impaired PI3K/AKT/mTOR ***:We established mouse models mimicked the complex pathological symptoms of COVID-19 patients with chronic ***-existing diseases could impair the inflammatory responses to SARS-CoV-2 infection,which further aggravated the pre-existing *** work provided valuable information to better understand the interplay between the primary diseases and SARS-CoV-2 infection.

关键词： Cardiovascular disease COVID-19 diabetes mellitus mouse model SARS-CoV-2

多媒体传感器网络中基于簇的QoS多路径路由协议

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重庆邮电大学学报（自然科学版） 2014年第5期26卷 593-597页

作者：吴笛董淑福康巧燕李尧赵亚金空军工程大学信息与导航学院陕西西安710077 北京军区空军司令部网管中心北京100038

针对多媒体传感器网络的应用,提出了一种基于簇的服务质量(quality of serve,QoS)多路径路由协议(cluster-based QoS multipath routing protocol,CQMRP),利用本地信息采取模糊控制的分簇方法对网络进行划分,然后在分层分簇的网络模型... 详细信息

针对多媒体传感器网络的应用,提出了一种基于簇的服务质量(quality of serve,QoS)多路径路由协议(cluster-based QoS multipath routing protocol,CQMRP),利用本地信息采取模糊控制的分簇方法对网络进行划分,然后在分层分簇的网络模型基础上,以带宽为QoS参数并提出节点饱和度和路径饱和度的概念,利用多路径并行传输流量实现拥塞避免、数据实时性传输和网络的高吞吐量;利用模糊控制的分簇方法实现网络的层次化管理和提高应用的可扩展性。仿真结果表明,该协议具有良好的实时性和可扩展性,并能有效延长网络生命周期。

关键词：多媒体传感器网络服务质量(QoS) 模糊控制簇多路径路由

Thioredoxin 2 Is a Novel E2-Interacting Protein That Inhibits the Replication of Classical Swine Fever Virus

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Thioredoxin 2 Is a Novel E2-Interacting Protein That Inhibit...

不同频率声波加载对SD大鼠行为学和海马5-羟色胺、多巴胺及D2受体表达的影响

第十届全国免疫学学术大会

作者： Su Li Jinghan Wang Wen-Rui He Shuo Feng Yongfeng Li Xiao Wang yajin Liao Hua-Yang Qin Lian-Feng Li Hong dong Yuan Sun Yuzi Luo Hua-Ji Qiu State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences

Purpose:The E2 protein of classical swine fever virus(CSFV)is an envelope glycoprotein that is involved in virus attachment and *** date,the E2-interacting cellular proteins and their involvement in viral replication have been poorly *** and Results:In this study,thioredoxin 2(Trx2)was identified to be a novel E2-interacting partner using yeast two-hybrid screening from a porcine macrophage c DNA ***2 is a mitochondrion-associated protein that participates in diverse cellular *** Trx2-E2 interaction was further confirmed by glutathione S-transferase(GST)pulldown,in situ proximity ligation and laser confocal *** thioredoxin domain of Trx2 and the asparagine at position37(N37)in the E2 protein were shown to be critical for the *** of the Trx2 expression in PK-15 cells by small interfering RNAs significantly promotes CSFV replication,and conversely,overexpression of Trx2 markedly inhibits viral replication of the wild-type(wt)CSFV and to a greater extent that of the CSFV N37D mutant,which is defective in binding *** wt CSFV but not the CSFV N37D mutant was shown to reduce the Trx2 protein expression in PK-15 ***,we demonstrated that Trx2 increases nuclear factor kappa B(NF-κB)promoter activity by promoting the nuclear translocation of the p65 subunit of NF-κ***,activation of the NF-κB signaling pathway induced by tumor necrosis factor alpha(TNF-α)significantly inhibits CSFV replication in PK-15 cells,whereas blocking the NF-κB activation in Trx2-overexpressing cells no longer suppresses CSFV ***:Taken together,our findings reveal that Trx2 inhibits CSFV replication via the NF-κB signaling pathway.

关键词： classical swine fever virus E2 protein thioredoxin 2 NF-κB virus-host interaction viral replication

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中国细胞生物学学报 2019年第8期41卷 1569-1577页

作者：邓志丹任扬赵押金陈东侯鳗王翔重庆大学生物工程学院

该文旨在探讨不同频率声波加载对SD大鼠抑郁症行为学、海马体分泌5-羟色胺(5-hydroxytryptamine, 5-HT)、多巴胺(dopamine, DA)和对多巴胺D2受体表达的影响。在音乐解析的基础上,对音乐的节奏要素进行了研究,运用作曲、编曲软件Finale2... 详细信息

该文旨在探讨不同频率声波加载对SD大鼠抑郁症行为学、海马体分泌5-羟色胺(5-hydroxytryptamine, 5-HT)、多巴胺(dopamine, DA)和对多巴胺D2受体表达的影响。在音乐解析的基础上,对音乐的节奏要素进行了研究,运用作曲、编曲软件Finale2011创作系列模拟声波。以大鼠常有的正常、麻醉、应激三种状态下的心率频率作为标准,分别模拟出频率为350 beats/min、300 beats/min、400 beats/min三种单音声波的音频文件加载于SD大鼠后,用试剂盒检测海马体左右两侧的5-HT和DA的浓度。免疫荧光强度分析D2受体的表达情况。结果显示,与对照组相比, 300 beats/min组的大鼠行为学变化更为积极,体重、糖水偏好率均明显增加,同时5-HT和DA浓度增加,分别增多0.098 8 ng/mL(P<0.01)、808.5 ng/mL(P<0.01);350 beats/min组和400 beats/min组行为学变化偏向抑郁,体重和糖水偏好率明显下降, 350 beats/min组5-HT和DA分别下降0.005 3 ng/mL(P<0.01)、308.5 ng/mL(P<0.01);400 beats/min组分别下降0.031 5 ng/mL(P<0.01)、1 067.5 ng/mL(P<0.01);此外,频率为300 beats/min的声波加载使D2受体表达明显加强(P<0.01),其他两组频率声波加载表达减弱。该研究结果得出,不同频率的声波加载对SD大鼠行为学变化、体重、糖水偏好率和5-HT、DA的分泌有不同的影响,对大鼠加载频率接近于大鼠麻醉状态心率时的声波,更有利于海马体行为学的变化和体重、糖水偏好的增加以及5-HT和DA的分泌和D2受体的表达。

关键词：频率 5-羟色胺多巴胺声波海马体

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变电站综合自动化系统的调试

河北电力技术 2008年第1期27卷 21-22页

作者：孙雅锦董祯祁志强石家庄供电公司石家庄050051 华北电力大学河北保定071003

介绍变电站综合自动化系统的技术要求,对系统的调试工作进行研究和总结,并提出系统调试中需要注意的问题,可为同类系统的调试工作提供参考。

关键词：变电站综合自动化调试无人值班