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Chinese Chemical Letters 2005年第6期16卷 815-818页

作者： QinWEI DanWU yahli JunHongWANG YanLIU BinBU SchoolofChemistry＆ChemicalEngineering JinanUniversityJinan250022 SchoolofChemistry＆ChemicalEngineering JinanUniversityJinan250022／／KeyLaboratoryofColloidandInterfaceChemistryShandongUniversityJinan250100

Based on the enhancement of resonance light scattering (RLS) of m-nitrophenylfluorone -Mo(VI) complex by protein, a novel method for protein microdetermination in Tween 20 microemulsion has been developed. Under optimum condition, the linear ranges of bovine serum albumin are 0 - 0.03 μg-mL -1 with detection limits of 3.10 ng-mL-1. Most of amino acids and metal ions do not interfere. The method can be applied to determination of residual protein in penicillin Na salt and penicillin G potassium salt samples with satisfactory results.

关键词： Protein microemulsion m-NPF-Mo(VI) complex penicillin resonance light scattering.

The M Protein of SARS-CoV: Basic Structural and Immunological Properties

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Genomics, Proteomics & Bioinformatics 2003年第2期1卷 118-130页

作者： JunWang SiqiLiu ChangqingZeng JianWang HuanmingYang YongwuHu JieWen LinTang HaijunZhang XiaoweiZhang yahli JingWang YujunHan GuoqingLi JianpingShi XiangjunTian FengJiang XiaoqianZhao BeijingGenomicsInstitute ChineseAcademyofSciencesBeijing101300China CollegeofLifeSciences PekingUniversityBeijing100871China JamesD.WatsonInstituteofGenomeSciences ZhijiangCampusZhejiangUniversityandHangzhouGenomicsInstituteHangzhou310008China

We studied structural and immunological properties of the SARS-CoV M (membrane) protein, based on comparative analyses of sequence features, phylogenetic investigation, and experimental results. The M protein is predicted to contain a triple-spanning transmembrane (TM) region, a single N-glycosylation site near its N-terminus that is in the exterior of the virion, and a long C-terminal region in the interior. The M protein harbors a higher substitution rate (0.6% correlated to its size) among viral open reading frames (ORFs) from published data. The four substitutions detected in the M protein, which cause non-synonymous changes, can be classified into three types. One of them results in changes of pI (isoelectric point) and charge, affecting antigenicity. The second changes hydrophobicity of the TM region, and the third one relates to hydrophilicity of the interior structure. Phylogenetic tree building based on the variations of the M protein appears to support the non-human origin of SARS-CoV. To investigate its immunogenicity, we synthesized eight oligopeptides covering 69.2% of the entire ORF and screened them by using ELISA (enzyme-linked immunosorbent assay) with sera from SARS patients. The results confirmed our predictions on antigenic sites.

关键词： SARS-CoV the M protein enzyme immunoassay antigenicity

The R Protein of SARS-CoV: Analyses of Structure and Function Based on Four Complete Genome Sequences of Isolates BJ01-BJ04

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Genomics, Proteomics & Bioinformatics 2003年第2期1卷 155-165页

作者： HaiyanSun XiaoweiZhang JunZhow SonggangLi JunWang JianWang ShenghBi HuanmingYang ZuyuanXu HaiqingZhang XiangjunTian JiaJi WeiLi yahli WeiTian LiftWang ZizhangZhang JingXu WeiWei JinguiZhu BeijingGenomiesInstitute ChineseAcademyofSciencesBeijing101800China CollegeofLifeSciences PekingUniversityBeijin9100871Ghina CenterofDiseaseControlandPrevention Beijin9100050China JgamesD.WatsonInstituteofGenomeSciences ZhijiangCampusZhejiangUniversityHangzhou310008China MedicalCollege Xi‘anJiaotongUniversityXi’an710049China

The R (replicase) protein is the uniquely defined non-structural protein (NSP) responsible for RNA replication, mutation rate or fidelity, regulation of transcription in coronaviruses and many other ssRNA viruses. Based on our complete genome sequences of four isolates (BJ01-BJ04) of SARS-CoV from Beijing, China, we analyzed the structure and predicted functions of the R protein in comparison with 13 other isolates of SARS-CoV and 6 other coronaviruses. The entire ORF (open-reading frame) encodes for two major enzyme activities, RNA-dependent RNA polymerase (RdRp) and proteinase activities. The R polyprotein undergoes a complex proteolytic process to produce 15 function-related peptides. A hydrophobic domain (HOD) and a hydrophilic domain (HID) are newly identified within NSP1. The substitution rate of the R protein is close to the average of the SARS-CoV genome. The functional domains in all NSPs of the R protein give different phylogenetic results that suggest their different mutation rate under selective pressure. Eleven highly conserved regions in RdRp and twelve cleavage sites by 3CLP (chymotrypsin-like protein) have been identified as potential drug targets. Findings suggest that it is possible to obtain information about the phy-logeny of SARS-CoV, as well as potential tools for drug design, genotyping and diagnostics of SARS.

关键词： SARS SARS-CoV RNA-dependent RNA polymerase RNA viruses proteolysis

腋窝淋巴结阴性浸润性乳腺癌分子分型的表达及预后分析

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中国肿瘤临床 2014年第12期41卷 793-796页

作者：张晓辉徐雅莉孙强潘博周易冬茅枫关竞红林燕中国医学科学院北京协和医院乳腺外科北京市100032

目的:探讨腋窝淋巴结阴性浸润性乳腺癌不同分子分型的分布,临床病理特征以及和预后的关系。方法:回顾性分析183例该类乳腺癌患者的临床病理资料,对管腔上皮(Luminal)型、基底样(Basal-1ike)型和HER-2过表达(over-expression)型乳腺癌患... 详细信息

目的:探讨腋窝淋巴结阴性浸润性乳腺癌不同分子分型的分布,临床病理特征以及和预后的关系。方法:回顾性分析183例该类乳腺癌患者的临床病理资料,对管腔上皮(Luminal)型、基底样(Basal-1ike)型和HER-2过表达(over-expression)型乳腺癌患者在年龄、肿瘤大小、临床分期和无瘤生存率(disease-free survival,DFS)、总生存率(overall survival,OS)方面进行统计分析。结果:不同分子亚型乳腺癌在年龄、肿瘤大小、临床分期方面无显著性差异。Luminal、Basal-like、HER-2过表达型的复发率分别为3.9%(4/102)、20.4%(10/49)、6.3%(2/32)(P=0.002);死亡率分别为2.0%(2/102)、6.1%(3/49)、3.1%(1/32)(P>0.05)。Ka-plan-Meier分析显示Basal-like型的DFS最低(P=0.002),Basal-like型的OS较低(P=0.39)。Cox多因素比例风险模型分析显示分子亚型对DFS存在显著影响(P=0.001)。结论:在腋窝淋巴结阴性浸润性乳腺癌患者中,不同分子亚型在年龄、肿瘤大小及临床分期方面无显著性差异,Basal-like型预后最差,分子分型是其独立预后指标。

关键词：乳腺癌分子分型生存预后

饮用水有机提取物对L02细胞增殖及细胞周期的影响

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贵阳医学院学报 2016年第3期41卷 294-297,303页

作者：岑延利杨光红桂晓玲张爱华李传美贵州医科大学公共卫生学院

目的:探讨饮用水有机提取物对人正常肝细胞(L02)的细胞增殖及细胞周期的影响。方法:体外培养L02细胞,将其分别暴露于培养液(空白对照组)、0.1%DMSO(溶剂对照组)和0.312 5、0.625 0、1.250 0、2.500 0、5.000 0 L/m L固相萃取法提取的饮... 详细信息

目的:探讨饮用水有机提取物对人正常肝细胞(L02)的细胞增殖及细胞周期的影响。方法:体外培养L02细胞,将其分别暴露于培养液(空白对照组)、0.1%DMSO(溶剂对照组)和0.312 5、0.625 0、1.250 0、2.500 0、5.000 0 L/m L固相萃取法提取的饮用水有机提取物(染毒组)中24、48及72 h,采用MTT法观察L02细胞的增殖情况,流式细胞仪检测细胞周期的构成比。结果:(1)与溶剂对照组比较,各染毒组细胞活力随着有机提取物浓度的增加及时间的延长而降低,差异有统计学意义(P<0.05);(2)随染毒剂量的增加,细胞周期中G0/G1期细胞比例逐渐下降,除24 h、48 h 0.312 5 L/m L染毒组外,其余剂量染毒组G0/G1期细胞比例均明显低于溶剂对照组(P<0.05);S期细胞比例则随有机提取物浓度的增加而上升,除24、48及72 h 0.312 5 L/m L的染毒组外,其余染毒组中S期细胞比例的增加与对照组比较,差异有统计学意义(P<0.05);染毒48 h、72 h后,L02细胞中G0/G1期细胞比例低于24 h染毒组(P<0.05);S期细胞比例则高于24 h染毒组(P<0.05)。结论:在本研究条件下,饮用水有机提取物可以阻滞L02细胞于S期,抑制细胞的增殖,且呈时间、剂量依赖性。

关键词：饮用水有机提取物肝细胞细胞增殖细胞周期