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Journal of Forestry Research 2020年第5期31卷 1843-1850页

作者： Fansuo Zeng Xiaoyi Li Rui Qie Leilei Li Minghao Ma yaguang zhan State Key Laboratory of Tree Genetics and Breeding Northeast Forestry UniversityHarbin 150040People’s Republic of China College of Life Science Northeast Forestry UniversityHarbin 150040People’s Republic of China First Affiliated Hospital Heilongjiang University of Chinese MedicineHarbin 150040People’s Republic of China

DNA methylation is widespread in plants and associated with plant development and defense ***,the relationship between DNA methylation and plant secondary metabolism has rarely been ***,when birch suspension cells were treated with 5-azacytidine(5-azaC),which blocks DNA methylation,triterpenoid accumulation was significantly promoted and antioxidant and defense enzymatic activity *** studying triterpenoid accumulation,0.1 mM azaC was optimal.A qRT-PCR assay revealed increased expression of genes encoding key triterpenoid biosynthetic *** of methylation polymorphisms at CCGG sites showed that the methylation level was lower in cells treated with *** results demonstrated that 5-azaC treatment led to an increase in the production of triterpenoids in cell cultures through a mechanism that involved in DNA methylation,which resulted in the induction of genes encoding the key *** study provides evidence of a relationship between DNA methylation and regulation of secondary metabolism.

关键词： 5-Azacytidine Methylation Suspension cells Triterpenoids biosynthesis

Molecular cloning and expression under abiotic stresses and hormones of the ethylene response factor Ⅶ gene FmRAP2.12 from Fraxinus mandshurica

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Journal of Forestry Research 2019年第4期30卷 1289-1300页

作者： Nansong Liang Lei Yu Chunhao Liu Ziqing Wang Xingtang Zhao yaguang zhan State Key Laboratory of Tree Genetics and Breeding Northeast Forestry University26 Hexing RoadHarbin 150040HeilongjiangPeople's Republic of China Department of Forest Bioengineering College of Life SciencesNortheast Forestry University26 Hexing RoadHarbin 150040HeilongjiangPeople's Republic of China

RELATED TO AP2.12(RAP2.12)is one of the Ethylene Response Factors(ERF)transcription factor and plays a key role in controlling plant root bending and responding to multiple abiotic stresses including hypoxia *** this study,FmRAP2.12 gene was isolated and characterized from Fraxinus mandshurica *** open reading frame(ORF)of FmRAP2.12 was 1170 bp and encoded a protein of 389 amino *** conserved domains,three-dimensional phylogenetic relationship of FmRAP2.12 was also *** real-time(qRT-PCR)analyzed the expression of FmRAP2.12 in different *** expression level of FmRAP2.12 was highest in roots followed by leaves,and lowest in male *** stress and hormone signal-induced expression was established using *** stress induced FmRAP2.12 to a double peak pattern:the first peak value was at 6 h and the second at 72 *** stress also induced FmRAP2.12 to a double peak pattern:the first at6 h and the second at 48 ***2.12 was up-regulated after initiation of gibberellic acid(GA3)treatment,with a one peak pattern at 24 ***2.12 may not respond to cold stress and Abscisic acid(ABA)*** transient overexpression of FmRAP2.12 caused the up-expression of downstream key genes of abiotic stress response and gibberellin *** research reveals the molecular characteristic and expression patterns under abiotic stress and hormone condition of FmRAP2.12,providing support for the genetic improvement of *** at a molecular level.

关键词： ERF RAP2.12 Gene clone Gene expression Fraxinus mandshurica

Regulatory effect of salicylic acid and methyl jasmonate supplementation on ergosterol production in Hericium erinaceus mycelia

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Journal of Forestry Research 2015年第1期26卷 71-77页

作者： Xiaodong Dai yaguang zhan Jiechi zhang Piqi zhang Zenghua Han Qingfang Ma Xianghui Kong Jianing Liu Yinpeng Ma College of Life Science Northeast Forestry University Microbiology Institute of Heilongjiang Provincial Academy of Sciences Institute of High Technology Heilongjiang Academy of Science

We evaluated the biomass and ergosterol content of Hericium erinaceus mycelium, and extracellular enzyme activities in H. erinaceus liquid culture following salicylic acid（SA） and methyl jasmonic acid（Me JA）supplementation. The optimal SA concentration was100 lmoláL-1, where the highest ergosterol content of 2.33 mgág-1was obtained following 6-day cultivation with100 lmoláL-1SA supplementation, and which was significantly higher than the unsupplemented control（p ／ 0.01）. Following 4-day supplementation with50 lmoláL-1Me JA, the highest ergosterol content obtained was 1.988 mgág-1, which was 25.8 % higher than the unsupplemented control. Our data indicate that SA and Me JA supplementation improves ergosterol content in *** mycelium.

关键词： Hericium erinaceus Ergosterol Salicylic acid Methyl jasmonate Inducers

Isolation,expression,and promoter analysis of FmWRKY7 and45:Two novel transcription factor genes from Fraxinus mandshurica related to cold-resistant

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Isolation,expression,and promoter analysis of FmWRKY7 and45:...

全国农业生物化学与分子生物学第十五届学术研讨会

作者： Liming He Liming He yaguang zhan yaguang zhan Life Science College Northeast Forestry University National Key Laboratory of Tree Genetics and Breeding Northeast Forestry University

Expression of genes under different various elicitors:Real-time PCR,Cloning of the full-Length cDNA sequence of FmWRKY7 and45:RT-PCR Amplification of the promoter region of Fm WRKY7and45:Genome Walker Main results:Under the cold condition,expression of FmWRKY7 and 45 were obviously different in different tissues,both of the two genes were down reglulated(2.05(wrky7)and 5.32(wrky45)fold)in the leaf,and up reglulated(12.24(wrky7)and1.69(wrky45)fold)in the stem(Fig1A).FmWRKY7

关键词： Fraxinus mandshurica WRKY transcription factor Gene Cloning Promoter Cloning

来源： cnki会议评论

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黄波罗的愈伤组织培养

东北林业大学学报 2007年第9期35卷 14-15,23页

作者：亓磊詹亚光东北林业大学哈尔滨150040 东北林业大学生命科学学院

通过组织培养技术研究了NAA、NAA与6-BA组合、培养基类型、培养基pH值、蔗糖质量浓度、接种量、培养时间及继代次数对黄波罗愈伤组织生长的影响。结果表明:单独使用NAA时,以1.0mg.L-1愈伤组织生长率最高;NAA与6-BA组合,以0.3mg.L-16-BA+... 详细信息

通过组织培养技术研究了NAA、NAA与6-BA组合、培养基类型、培养基pH值、蔗糖质量浓度、接种量、培养时间及继代次数对黄波罗愈伤组织生长的影响。结果表明:单独使用NAA时,以1.0mg.L-1愈伤组织生长率最高;NAA与6-BA组合,以0.3mg.L-16-BA+1.0mg.L-1NAA最好,其生长量明显大于单独使用NAA的处理。黄波罗愈伤组织最佳的培养体系为:NT+0.3mg.L-16-BA+1.0mg.L-1NAA,培养基pH值为6.3左右,蔗糖质量浓度为20g.L-1,接种量0.4g,继代时间为20d,愈伤组织生长量在培养4代后达到最大,达2.14g左右。

关键词：黄波罗愈伤组织继代培养

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不同白桦优树组培快繁体系的筛选

东北林业大学学报 2009年第11期37卷 17-20,23页

作者：占爱瑶由香玲詹亚光东北林业大学哈尔滨150040

用已建立的白桦离体再生培养体系对20个白桦杂交优树(Betula platyhylly×Betula pendula)腋芽进行了离体培养,比较它们腋芽再生和愈伤组织再生情况,并分析了无性繁殖的变异情况。结果表明:20个优树腋芽启动率存在的差异较大,最高的为4... 详细信息

用已建立的白桦离体再生培养体系对20个白桦杂交优树(Betula platyhylly×Betula pendula)腋芽进行了离体培养,比较它们腋芽再生和愈伤组织再生情况,并分析了无性繁殖的变异情况。结果表明:20个优树腋芽启动率存在的差异较大,最高的为4号和7号优树,为75.0%,最低的为17号优树,仅24.4%;3号和16号优树的启动率较高,分别为63.1%、61.3%;3号和16号优树在腋芽增殖和愈伤组织再生途径中长势都比其它植株好,选择它们用于工厂化育苗,可以满足造林和生产需要。

关键词：白桦腋芽增殖愈伤组织再生工厂化育苗造林

水曲柳促生蜡样芽胞杆菌SQL0164的分离鉴定及其根表定殖分析

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中南林业科技大学学报 2020年第10期40卷 61-69页

作者：刘悦詹亚光牛犇东北林业大学生命科学学院黑龙江哈尔滨150040 东北林业大学林木遗传育种国家重点实验室黑龙江哈尔滨150040

【目的】以水曲柳促生细菌(蜡样芽胞杆菌SQL0164)为研究对象,揭示菌株SQL0164在水曲柳根部定殖的动态变化规律,明确其与定殖相关的生物学性状。【方法】梯度稀释法分离纯化细菌菌株;灌根接种细菌分离物至苗期水曲柳植株,筛选促生效果显... 详细信息

【目的】以水曲柳促生细菌(蜡样芽胞杆菌SQL0164)为研究对象,揭示菌株SQL0164在水曲柳根部定殖的动态变化规律,明确其与定殖相关的生物学性状。【方法】梯度稀释法分离纯化细菌菌株;灌根接种细菌分离物至苗期水曲柳植株,筛选促生效果显著的菌株;通过16S rRNA基因序列系统发育分析与生理生化反应鉴定促生菌株SQL0164的分类学地位;采用平板菌落计数与扫描电镜确定菌株SQL0164在水曲柳根表的定殖量与定殖位点;在不同培养基中观测菌株SQL0164形成的生物膜形态,采用结晶紫染色法对生物膜进行定量;离体条件下,检测菌株SQL0164的运动性。【结果】筛选出对水曲柳促生效果显著的菌株SQL0164,与对照相比,经菌株SQL0164处理21 d的水曲柳幼苗的鲜质量和株高分别增加了51.2%与15.4%;经鉴定,菌株SQL0164为蜡样芽胞杆菌;菌株SQL0164能够通过形成微菌落定殖于水曲柳根表皮细胞表面,且在水曲柳根表的定殖量随着寄主的生长呈现逐渐增加的趋势;菌株SQL0164在0.1×TSB、0.1×LB、MSGG以及0.5×NB培养基中能够形成致密的漂浮型生物膜,在LBGM培养基中培养72 h后,菌株SQL0164形成的潜底型生物膜的生物量最高;菌株SQL0164的菌落分别于接种后8.5 h和24 h遍布整个swimming与swarmming平板,显示出该菌株具有显著的运动能力。【结论】蜡样芽胞杆菌SQL0164能够显著促进苗期水曲柳植株的生长,该菌株能够通过形成微菌落稳定定殖于水曲柳根表皮细胞表面,且其定殖量随着寄主的生长呈现逐渐增加的趋势,离体条件下,菌株SQL0164表现出显著的生物膜形成能力与运动能力。

关键词：水曲柳植物促生根细菌定殖生物膜运动性

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植物抗旱机理及相关基因研究进展

生物技术通报 2009年第2期25卷 11-17页

作者：徐云刚詹亚光东北林业大学生命科学学院哈尔滨150040

提高植物的抗旱能力已经成为现代植物研究工作中的关键问题之一。近年来,随着分子生物学的应用与发展,该领域的研究也已引起国内外学者广泛的兴趣和重视,在抗旱机理研究及相关基因克隆及表达调控方面已取得可喜进展。对植物抗旱机理及... 详细信息

提高植物的抗旱能力已经成为现代植物研究工作中的关键问题之一。近年来,随着分子生物学的应用与发展,该领域的研究也已引起国内外学者广泛的兴趣和重视,在抗旱机理研究及相关基因克隆及表达调控方面已取得可喜进展。对植物抗旱机理及相关基因研究进展做了详细的综述,并对以后植物抗旱的研究做了展望。

关键词：干旱胁迫抗旱机理渗透调节离子区域化转录因子

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茶条槭悬浮培养的动力学

林业科学 2012年第10期48卷 18-23页

作者：董杰詹亚光任健东北林业大学生命科学学院哈尔滨150040

对茶条槭细胞培养动力学进行研究,在培养周期内不同的培养阶段测定茶条槭细胞生长和培养基中碳源、氮源、磷源的消耗,电导率的变化,以及细胞的鲜质量与干质量的变化,从而了解细胞生长、营养消耗与次生代谢产物积累的基本规律,为建立结... 详细信息

对茶条槭细胞培养动力学进行研究,在培养周期内不同的培养阶段测定茶条槭细胞生长和培养基中碳源、氮源、磷源的消耗,电导率的变化,以及细胞的鲜质量与干质量的变化,从而了解细胞生长、营养消耗与次生代谢产物积累的基本规律,为建立结构化动力学模型奠定基础。研究结果表明:1)茶条槭细胞悬浮培养周期约为15天,经过15天的悬浮培养,最大生物量和没食子酸含量分别达到了11.3g·L-1和0.49%。细胞的最大比生长速率和没食子酸的最大比生成速率分别为0.541d-1和0.682d-1。没食子酸的比生成速率当细胞比生长速率在0.3～0.4d-1时较高。没食子酸的积累和茶条槭细胞的生长模型为部分生长偶联型。2)培养过程中培养基的电导率逐渐下降,到第21天时达到最低点,之后电导率略微上升。3)经过15天的培养后,培养基中磷源和蔗糖,消耗殆尽;在细胞培养前期细胞吸收铵盐较快,12天时已消耗殆尽,而细胞吸收硝酸盐开始速度较慢,6天细胞开始大量吸收硝酸盐,15天时培养基中的硝酸盐达到最低值。

关键词：茶条槭悬浮培养没食子酸细胞生长动力学

Effect of MeJA and Light on the Accumulation of Betulin and Oleanolic Acid in the Saplings of White Birch (<i>Betula platyphylla</i>Suk.)

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American Journal of Plant Sciences 2013年第12期4卷 7-15页

作者： Jing Yin Hongsi Ma Yuan Gong Jialei Xiao Lichao Jiang yaguang zhan Chunxiao Li Chunlin Ren Yuanbiao Yang College of Life Science Northeast Forestry University Harbin China Heilongjiang Academy of Agricultural Sciences Harbin China Maoershan Experimental Forestry Center Northeast Forestry University Harbin China

In this study, we investigated the effect of different types of light and MeJA treatment on the accumulation of betulin and oleanolic acid in various organs of white birch. Our results showed that betulin and oleanolic were accumulated mainly in the stalk skin. The content of both substances in the stalk skin was significantly affected by seasons with a peak accumulation in August. The content of oleanolic and betulin was significantly decreased in the stem skin treated with 4 types of light (red, yellow, blue and green) compared with the plant with normal illumination. In contrast, oleanolic acid in leaves was increased by 13.28 folds when the white birch was treated with green light. Betulin was increased by 1.959 folds in leaves of white birch treated with blue light. The highest content of betulin and oleanolic acid in various organs of birch with appropriate shading treatment (light transmittance: 50%) was increased by 45.09% and 30.50%, respectively, in comparison with those with non-shading treatment. Content of oleanolic acid and betulin can be significantly improved in various parts of birch after treatment with different concentration of MeJA. The study lays the foundation to metabolic regulation of oleanolic acid and betulin in birch.

关键词： Birch (Betula platyphylla Suk.) Betulin Oleanolic Acid Light MeJA