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Decabromodiphenyl ether induces the chromosome association disorders of spermatocytes and deformation failures of spermatids in mice

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Journal of Environmental Sciences 2024年第4期138卷 531-542页

作者： Jinglong Xue Xiangyang Li yafei chi Leqiang Gao Yue Zhang Yan Wang Moxuan Zhao Jialiu Wei Zhixiong Shi Xianqing Zhou Department of Toxicology and Hygienic Chemistry School of Public HealthCapital Medical UniversityBeijing 100069China Beijing Key Laboratory of Environmental Toxicology Capital Medical UniversityBeijing 100069China Laboratory Animal Center Capital Medical UniversityBeijing 100069China Key Laboratory of Cardiovascular Epidemiology&Department of Epidemiology Fuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China

The environmental presence of decabromodiphenyl ether(BDE-209),which is toxic to the male reproductive system,is *** current study investigated its mechanism of toxicity in *** results showed,that BDE-209 induced DNA damage,decreased the expression of the promoter of meiosis spermatogenesis-and oogenesis-specific basic helix-loop-helix 1(Sohlh1),meiosis related-factors Lethal(3)malignant brain tumor like 2(L3MBTL2),PIWI-like protein 2(MILI),Cyclin-dependent kinase 2(CDK2),Cyclin A,synaptonemal complex protein 1(SYCP1)and synaptonemal complex protein 3(SYCP3),and caused spermatogenic cell apoptosis,resulting in a decrease in sperm quantity and ***,BDE-209 downregulated the levels of anaphase-promoting complex/cyclosome(APC/C),increased the expression of PIWI-like protein 1(MIWI)in the cytoplasm of elongating spermatids,and decreased the nuclear levels of RING finger protein 8(RNF8),ubiquitinated(ub)-H2A/ub-H2B,and Protamine 1(PRM1)/Protamine 2(PRM2),while increasing H2A/H2B nuclear levels in *** reproductive toxicity was persistent for 50 days following the withdrawal of BDE-209 *** results suggested that BDE-209 inhibits the initiation of meiosis by decreasing the expression of ***,the reduced expression of L3MBTL2 inhibited the formation of chromosomal synaptonemal complexes by depressing the expression of meiosis regulators affecting the meiotic progression and also inhibited histone ubiquitination preventing the replacement of histones by protamines,by preventing RNF8 from entering nuclei,which affected the evolution of spermatids into mature sperm.

关键词： BDE-209 Meiosis L3MBTL2 Sperm RNF8 Histone ubiquitination

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长爪沙鼠性成熟前后生殖系统的发育情况

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实验动物科学 2024年第4期41卷 22-28页

作者：池亚菲彭博雅马俊李梦陈柏安仵毅卢静首都医科大学实验动物部北京100069 首都医科大学基础医学院实验动物学学系北京100069 首都医科大学基础医学院神经再生修复研究北京市重点实验室北京100069

目的深入探究长爪沙鼠性成熟过程中生殖系统的发育情况,为其性成熟的确切时间提供直接研究证据。方法本研究选取6~14周龄长爪沙鼠共150只,按6、8、10、12和14周龄分为5组,每组雌雄各15只,称量体质量并采集卵巢、子宫、睾丸、附睾样本,... 详细信息

目的深入探究长爪沙鼠性成熟过程中生殖系统的发育情况,为其性成熟的确切时间提供直接研究证据。方法本研究选取6~14周龄长爪沙鼠共150只,按6、8、10、12和14周龄分为5组,每组雌雄各15只,称量体质量并采集卵巢、子宫、睾丸、附睾样本,测量各样本组织湿重并计算脏器指数;镜下观察卵巢外观形态;细胞计数仪检测精子数量;每组随机选取6个样本进行HE染色,观察卵巢、睾丸、附睾的组织发育情况。结果雌性沙鼠10周龄时卵巢湿重和卵巢指数均明显增加,且10~14周龄期间无明显变化,子宫湿重和子宫指数均呈阶段性变化;镜下观察外观,10周龄、12周龄、14周龄组黄体发生率分别为46.7%、60%、73.3%;组织切片显示,10周龄起可见明显黄体。雄性沙鼠在6~10周龄阶段睾丸湿重随周龄的增长而逐渐增加,10周龄后无明显变化;睾丸指数在8及12周龄时明显增加,14周龄时明显减小;6~12周龄阶段附睾湿重和附睾指数均随周龄的增长而逐渐增加,14周龄时附睾湿重无明显变化,附睾指数明显减小。细胞计数仪结果显示,6周龄时精子数量为0,10周龄与8周龄相比,附睾内精子数量明显增加,10~14周龄期间无明显变化。组织切片显示,8周龄起,睾丸和附睾内开始出现少量精子,10周龄起可见大量精子。结论随着周龄的增长,雌性沙鼠卵巢在10周龄左右卵泡开始发育成熟并排出卵子,达到性成熟,具备繁殖能力。雄性沙鼠8周龄左右开始产生精子,进入初情期,10周龄左右睾丸发育稳定,精子达最大数量,达到性成熟,此后附睾继续发育,12周龄左右发育稳定。

关键词：长爪沙鼠卵巢子宫睾丸附睾性成熟

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Kinetics of the accumulation of group 2 innate lymphoid cells in IL-33-induced and IL-25-induced murine models of asthma:a potential role for the chemokine CXCL16

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Cellular & Molecular Immunology 2019年第1期16卷 75-86页

作者： Yan Li Shihao Chen yafei chi Yiran Yang Xiwen Chen Huating Wang Zhe Lv Jingjing Wang Linjie Yuan Ping Huang Kewu Huang Chris JCorrigan Wei Wang Sun Ying Department of Immunology School of Basic Medical SciencesCapital Medical UniversityBeijingP.R.China Department of Laboratory Animal Sciences Capital Medical UniversityBeijingChina Department of Respiratory and Critical Care Medicine Beijing Chao-Yang HospitalCapital Medical University and Beijing Institute of Respiratory MedicineBeijingChina Faculty of Life Sciences&Medicine School of Immunology and Microbial SciencesAsthma UK Centre in Allergic Mechanisms of AsthmaKing’s College LondonLondonUK

ILC2s are implicated in asthma pathogenesis, but little is known about the mechanisms underlying their accumulation in *** investigated the time course of ILC2 accumulation in different tissues in murine models of asthma induced by a serial per-nasalchallenge with ovalbumin (OVA), house dust mice (HDM), IL-25 and IL-33 and explored the potential roles of ILC2-attractingchemokines in this phenomenon. Flow cytometry was used to enumerate ILC2s at various time points. The effects of cytokines andchemokines on ILC2 migration were measured in vitro using a chemotaxis assay and in vivo using small animal imaging. Comparedwith saline and OVA challenge, both IL-25 and IL-33 challenge alone induced significant accumulation of ILC2s in the mediastinallymph nodes, lung tissue and bronchoalveolar lavage fluid of challenged animals, but with a distinct potency and kinetics. In vitro,IL-33 and CXCL16, but not IL-25 or CCL25, directly induced ILC2 migration. Small animal in vivo imaging further confirmed that asingle intranasal provocation with IL-33 or CXCL16 was sufficient to induce the accumulation of ILC2s in the lungs followinginjection via the tail vein. Moreover, IL-33-induced ILC2 migration involved the activation of ERK1/2, p38, Akt, JNK and NF-κB, whileCXCL16-induced ILC2 migration involved the activation of ERK1/2, p38 and Akt. These data support the hypothesis that epitheliumderived IL-25 and IL-33 induce lung accumulation of ILC2s, while IL-33 exerts a direct chemotactic effect in this process. AlthoughILC2s express the chemokine receptors CXCR6 and CCR9, only CXCL16, the ligand of CXCR6, exhibits a direct chemoattractanteffect.

关键词： ILC2s Asthma Accumulation IL-33 IL-25 Chemokine

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Hypermethylation of GSTM5 and its effect on oxidation in myelodysplastic syndrome

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iLABMED 2024年第2期2卷 108-124页

作者： chi Wang yafei Yu Qing Chang Tengteng Dong Liye Wang Mianyang Li Department of Clinical Laboratory Medicine The First Medical CenterChinese PLA General HospitalBeijingChina

Background:Hypermethylation of glutathione-S-transferase 5(GSTM5)and its effect on oxidation in the pathogenesis of myelodysplastic syndrome(MDS)were ***:GSTM5 methylation was detected in bone marrow(BM)samples from MDS patients,acute myeloid leukemia(AML)patients,and control in-dividuals using methylation-specific PCR and MassARRAY *** sequencing PCR was performed to verify methylation levels,while mRNA levels were determined using reverse transcription polymerase chain *** between GSTM5 methylation and clinical parameters were *** MDS cell line,SKM-1,was treated with decitabine,buthionine sulfoximine,or overexpression of GSTM5,and the glutathione level and cell viability were ***:The MassARRAY analysis revealed significant differences in GSTM5 methylation levels between the MDS and control ***5 methylation levels were significantly increased in the high-risk subgroup and showed a significant association with MDS progression to AML(hazard ratio=3.6).Levels of GSTM5 mRNA were significantly decreased in the MDS group,exhibiting a negative correlation with the GSTM5 gene methylation *** BM HS-5 cells exhibited significantly lower levels of GSTM5 methylation than SKM-1 *** of GSTM5 in SKM-1 cells or treatment with buthionine sulfoximine or decitabine resul-ted in inhibition of proliferation and significantly decreased glutathione ***:GSTM5 plays an anti-oxidative role in MDS and the tumor suppressor effect of GSTM5 may be mediated by reducing glutathione ***5 hypermethylation and low levels of GSTM5 expression may be prog-nostic markers for MDS.

关键词： glutathione GSTM5 methylation myelodysplastic syndrome prognosis

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Kinetics of accumulation of group 2 innate lymphoid cells in IL-33 and IL-25 induced murine surrogates of asthma:potential role of the chemokine CXCL16

Kinetics of accumulation of group 2 innate lymphoid cells in...

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第十二届全国免疫学学术大会

作者： Li Yan Chen Shihao chi yafei Lv Zhe Wang Jingjing Yuan Linjie Huang Ping Huang Kewu Corrigan Chris J. Wang Wei Sun Ying Capital Medical University Beijing Chao-Yang Hospital Capital Medical University & Beijing Institute of Respiratory Medicine King's College London MRC & Asthma UK Centre in Allergic Mechanisms of Asthma

Background:ILC2 s are implicated in asthma pathogenesis but little is known about the mechanisms of their accumulation in the *** investigated the time courses of accumulation of ILC2 s into the lungs and other tissues in murine surrogates of asthma induced by serial per-nasal challenge with ovalbumin(OVA),IL-25 and IL-33 and the potential role of ILC2-attracting chemokines in this ***:Flow cytometry was used to enumerate ILC2 s in the bone marrow,thymus,spleen,mesenteric lymph nodes(Mes LN),mediastinal lymph nodes(Med LN),lung tissue and bronchoalveolar lavage fluid(BALF) of challenged animals at various *** effects of cytokines and chemokines on ILC2 migration were measured in vitro using a chemotaxis assay and in vivo using small animal ***:Compared with saline and OVA challenge,both IL-25 and IL-33 challenge alone induced significant accumulation of ILC2 s in Med LN,lung tissue and BALF of challenged animals,but with distinct potency and *** vitro,IL-33 and CXCL16,but not IL-25 or CCL25 directly induced ILC2 *** animal in vivo imaging further confirmed that a single intranasal provocation with IL-33 or CXCL16 was sufficient to induce accumulation of ILC2 s injected via a tail vein into the ***,IL-33 induced ILC2 migration involving activation of ERK1/2,p38,Akt,JNK and NF-κB while CXCL16 induced ILC2 migration involving activation of ERKl/2,p38 and ***:The data support the hypothesis that airways epithelium-derived IL-25 and IL-33 induce lung accumulation of ILC2 s,in the latter case at least partly by direct *** lung ILC2 s expressed the chemokine receptors CXCR6 and CCR9,only the ligand CXCL16 exhibited a direct chemoattractant effect.

关键词： ILC2s asthma accumulation IL-33 IL-25 chemokine

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近50年中国东部林区最高气温序列

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林业科学 2013年第8期49卷 1-9页

作者：邬宏张称意王立新池亚飞内蒙古大学环境与资源学院呼和浩特010021 国家气候中心北京100081

运用线性回归插补途径和RHtest方法分别对1961—2010年我国东部林区853个站点的日最高气温资料进行插补和均一化检验订正,建立完整且均一的日最高气温数据集。在此基础上,对比分析我国东部林区近50年来最高气温订正前后的变化特征。结... 详细信息

运用线性回归插补途径和RHtest方法分别对1961—2010年我国东部林区853个站点的日最高气温资料进行插补和均一化检验订正,建立完整且均一的日最高气温数据集。在此基础上,对比分析我国东部林区近50年来最高气温订正前后的变化特征。结果表明:订正后东部林区年及四季(春、夏、秋、冬)平均最高气温每10年分别增加0.171,0.233,-0.002,0.201和0.263℃,订正后整个区域年平均最高气温均呈上升趋势,而订正前有部分站点呈降温趋势;春、秋、冬3季平均最高气温在整个林区几乎全部呈现上升趋势,夏季东部林区多数站点呈降温趋势,尤其是长江和黄河流域之间的区域。

关键词：日最高气温缺测值插补均一性订正 RHtest 气候变化中国东部林区

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大豆卵磷脂联合大豆异黄酮改善大鼠学习记忆损伤及脑血管保护作用

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首都医科大学学报 2021年第6期42卷 993-999页

作者：余辉艳池亚菲李红睿王轩周雪阳肖荣席元第首都医科大学公共卫生学院营养与食品卫生学学系北京100069 首都医科大学实验动物部北京100069 北京市顺义区妇幼保健院医务科北京101300

目的观察大豆卵磷脂(soy lecithin,SL)和大豆异黄酮(soy isoflavone,SIF)改善大鼠学习记忆损伤及脑血管保护的联合作用,探讨二者的最佳联合剂量,为明确大豆活性物质的神经保护作用及其联合效应提供科学依据。方法本研究使用SPF级的成年... 详细信息

目的观察大豆卵磷脂(soy lecithin,SL)和大豆异黄酮(soy isoflavone,SIF)改善大鼠学习记忆损伤及脑血管保护的联合作用,探讨二者的最佳联合剂量,为明确大豆活性物质的神经保护作用及其联合效应提供科学依据。方法本研究使用SPF级的成年雄性Wistar大鼠,采用两因素三水平正交实验设计分为对照组、β淀粉样肽(β-amyloid,Aβ)模型组和9个不同的SL+SIF剂量组合干预组。应用水迷宫、脑血管HE染色、酶联免疫吸附测定(enzyme-linked immunosorbent assay,ELISA)以及酶法试剂盒等方法,对大鼠学习记忆能力、脑血管病理损伤、体内氧化损伤水平以及氧化还原平衡体系等相关指标进行检测。结果Aβ模型组大鼠逃避潜伏期显著延长(P<0.05),脑血管组织形态异常改变明显,并且伴有体内丙二醛(malondialdehyde,MDA)和3-硝基酪氨酸(3-nitrotyrosine,3-NT)显著上升(P<0.05),谷胱甘肽与氧化型谷胱甘肽的比值(ratio of glutathione/oxidized glutathione,GSH/GSSG)显著下降(P<0.05)。相反,SL+SIF各干预组与Aβ模型组比较,二者对上述改变均有不同程度的保护作用。SL(40 mg·kg^(-1)·d^(-1))+SIF(50 mg·kg^(-1)·d^(-1))是改善大鼠学习记忆、保护脑血管以及调节体内氧化还原平衡体系的最佳联合剂量。结论SL+SIF可以显著改善Aβ介导的大鼠学习记忆损伤,其神经保护作用可能与二者发挥抗氧化活性拮抗Aβ介导的脑血管损伤有关。二者的神经保护作用具有协同效应,SL(40 mg·kg^(-1)·d^(-1))+SIF(50 mg·kg^(-1)·d^(-1))是大鼠神经保护的最佳联合剂量。

关键词：大豆卵磷脂大豆异黄酮联合学习记忆损伤 β淀粉样肽

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心肌细胞条件性Ppara敲除小鼠模型中他莫昔芬安全有效剂量的筛选

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实验动物科学 2021年第4期38卷 16-21页

作者：王晶晶池亚菲乔欣卢静首都医科大学北京100069

目的探究不同的他莫昔芬(tamoxifen)给药剂量对小鼠心肌细胞过氧化物酶体增殖物激活受体α(peroxisome proliferator-activated receptorα,PPARα)基因Ppara敲除效率及心脏功能的影响,建立稳定有效的可诱导型心肌细胞特异性Ppara敲除... 详细信息

目的探究不同的他莫昔芬(tamoxifen)给药剂量对小鼠心肌细胞过氧化物酶体增殖物激活受体α(peroxisome proliferator-activated receptorα,PPARα)基因Ppara敲除效率及心脏功能的影响,建立稳定有效的可诱导型心肌细胞特异性Ppara敲除小鼠。方法 Ppara^(fl/fl)小鼠与Myh6-ERT2Cre小鼠进行交配及回交得到Ppara^(fl/fl,myh6-ERT2Cre)小鼠及同窝对照Ppara^(fl/fl)小鼠。将Ppara^(fl/fl,myh6-ERT2Cre)小鼠随机分为对照组(腹腔注射含20%乙醇的他莫昔芬玉米油溶液)、A组(单次腹腔注射他莫昔芬2 mg/只)、B组(20 mg/kg,连续注射他莫昔芬5 d)和C组(40 mg/kg,连续注射他莫昔芬5 d),n=8。两周后,采用小动物超声监测小鼠的心功能,病理Masson三色染色观察心肌纤维化的情况,Real-time qPCR和Western blot检测心肌组织中PPARα的表达改变。结果与对照组相比,A组小鼠的心功能与对照组没有显著差异,但Real-time qPCR显示心肌中Ppara的敲除效率不佳;B组,心脏超声监测和Masson染色结果显示此给药剂量对小鼠心脏功能没有影响,但Real-time qPCR和Western blot均显示心肌组织中Ppara敲除充分;而C组,虽然Real-time qPCR结果显示心肌组织中Ppara的敲除充分,但心脏超声和Masson染色结果显示此给药剂量对小鼠的心脏功能有一定程度的损伤。结论连续5 d给予Ppara^(fl/fl,myh6-ERT2Cre)小鼠腹腔注射20 mg/kg他莫昔芬可以充分诱导心肌细胞中Ppara的敲除,成功建立可诱导型心肌细胞特异性Ppara敲除(Ppara^(△CM))小鼠。

关键词： PPARα 他莫昔芬 Ppara^(△CM)

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高校实验动物行业非事业编制职工归属感调查及增强机制浅探

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实验动物科学 2020年第6期37卷 59-61,65页

作者：李胜利池亚菲孟霞卢静首都医科大学实验动物部北京100069

归属感作为一种社会心理资源对高校非事业编制职工的思想和行为表现具有重要的影响。本研究借鉴Goodenow编制的归属感量表,从归属感、认同和学校依恋三个维度对实验动物行业非事业编制人员进行了调查。结果表明,实验动物行业非事业编制... 详细信息

归属感作为一种社会心理资源对高校非事业编制职工的思想和行为表现具有重要的影响。本研究借鉴Goodenow编制的归属感量表,从归属感、认同和学校依恋三个维度对实验动物行业非事业编制人员进行了调查。结果表明,实验动物行业非事业编制人员的归属感呈现出明显的两极分化现象:学历较低的工勤人员对学校的归属感较强而学历稍高的技术岗位人员归属感偏弱。在对调研结果分析的基础上,提出提升高校实验动物行业非事业编制人员归属感的3条措施:激励政策公平化、激励方式丰富化和管理措施科学化。

关键词：非事业编制职工高校实验动物行业归属感激励政策

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Beehive: low-cost content subscription service using cloudlets

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Science china(Information Sciences) 2013年第7期56卷 194-209页

作者： XING YuanJian YANG Zhi CHEN chi DAI yafei Department of Computer Science Peking University

In recent years, Content Subscription Service (CSS) is showing a notable success in helping users fetch unpopular data in P2P networks. It works by maintaining a storage pool to pull the subscribed file and then redistribute them to the requestor. However, existing commercial CSS incurs huge bandwidth/equipment cost due to introducing a third-party cloud storage. We present Beehive, a low-cost yet efficient CSS, which leverages the idle node bandwidth/storage (i.e., cloudlets) in the original P2P system to form an inherent cloudlet storage. Since P2P nodes are geographically distributed, highly dynamic and heterogeneous, Beehive adopts three key techniques to achieve high performance. First, by applying random linear network coding, multiple seeders can upload the same file simultaneously without complicated wide-area coordination. Second, a model is developed to set the coding parameters so that the cloudlet storage achieves guaranteed performance in file availability and data distribution efficiency. Finally, by performing task outsourcing, overloaded seeders quickly offload their tasks to idle peers, which effectively balances the load and utilizes the system resources. We implement Beehive with 29,000 lines of C++ codes and deploy it in a real P2P file-sharing system. Results show that Beehive improves the success ratio of pulling peer-side files from 38.7% (traditional online download) to 84.3% (users offline waiting for less than three days), and provides users with over 200 kB/s download speed.

关键词： content subscription service peer-to-peer low-cost cloudlet-based storage

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