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生物学教学 2024年第9期49卷 86-88页

作者：林明强福建省南安国光中学

对分离定律复习的拓展，总体上离不开杂合子表型分析。一对等位基因决定的相对性状中，杂合子表现出什么样的表型，既受基因型控制，同时也受到环境的影响。在一轮复习教学中，可以杂合子的表型为主线，整合相关题目，引导学生进行深度... 详细信息

对分离定律复习的拓展，总体上离不开杂合子表型分析。一对等位基因决定的相对性状中，杂合子表现出什么样的表型，既受基因型控制，同时也受到环境的影响。在一轮复习教学中，可以杂合子的表型为主线，整合相关题目，引导学生进行深度学习。

关键词：一对等位基因杂合子表现型一轮复习

The near-infrared dye IR-61 restores erectile function in a streptozotocin-induced diabetes model via mitochondrial protection

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Asian Journal of Andrology 2021年第3期23卷 249-258页

作者： Xiao-Feng Yue Chong-Xing Shen Jian-Wu Wang lin-Yong Dai Qiang Fang Lei Long Yi Zhi Xue-Ru Li ya-Wei Wang Gu-Fang Shen Zu-Juan Liu Chun-Meng Shi Wei-Bing Li Departm ent of Urology The Third Affiliated Hospital of Chongqing Medical University(Gener Hospital)Chongqing 401120China lnstitute of Rocket Force Medicine State Key Laboratory of TraumaBurns and Combined InjuryThird Military Medical UniversityChongqing 400038China Department of Ophthalmology The Third Affiliated Hospital of Chongqing M edical University(Gener Hospital)Chongqing 401120China

This study aimed to evaluate the therapeutic effect of IR-61,a novel mitochondrial heptamethine cyanine dye with antioxidant effects,on diabetes mellitus-induced erectile dysfunction(DMED).Eight-week-old male Sprague-Dawley rats were intraperitoneally injected with streptozotocin(STZ)to induce type 1 *** weeks after STZ injection,all rats were divided into three groups:the control group,DM group,and DM+IR-61 *** the DM+IR-61 group,the rats were administered IR-61(1.6 mg kg-1)twice a week by intravenous *** week 13,erectile function was evaluated by determining the ratio of the maximal intracavernous pressure to mean arterial pressure,and the penises were then harvested for fluorescent imaging,transmission electron microscopy,histological examinations,and Western blot ***-body imaging suggested that IR-61 was highly accumulated in the penis after intravenous ***-61 treatment significantly improved the maximal ICP of diabetic ***,IR-61 ameliorated diabetes-induced inflammation,apoptosis,and phenotypic transition of corpus cavernosum smooth muscle cells(CCSMCs)in penile ***-61 also attenuated mitochondrial damage,reduced reactive oxygen species production in the corpus cavernosum and upregulated sirtuinl(SIRT1),sirtuin3(SIRT3),nuclear factor(erythroid-derived 2)-like 2(Nrf2),and heme oxygenase expression in penile *** conclusion,IR-61 represents a potential therapeutic option for DMED by protecting the mitochondria of CCSMCs,which may be mediated by activation of the SIRT1,SIRT3,and Nrf2 pathways.

关键词： corpus cavernosum smooth muscle cells diabetes erectile dysfunction IR-61 mitochondria

慢性阻塞性肺疾病急性加重期继发肺部真菌感染的列线图预测模型

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中国呼吸与危重监护杂志 2024年第2期23卷 77-85页

作者：林希黄娜成都医学院第一附属医院呼吸与危重症医学科四川成都610500

目的探究慢阻肺急性加重患者继发肺部真菌感染的危险因素,应用列线图构建并验证用于辅助临床预测慢阻肺急性加重患者发生肺部真菌感染概率的可视化工具。方法采用回顾性队列研究的方法,收集2021年1月—2021年12月期间,于成都医学院第一... 详细信息

目的探究慢阻肺急性加重患者继发肺部真菌感染的危险因素,应用列线图构建并验证用于辅助临床预测慢阻肺急性加重患者发生肺部真菌感染概率的可视化工具。方法采用回顾性队列研究的方法,收集2021年1月—2021年12月期间,于成都医学院第一附属医院呼吸与危重医学科住院治疗的慢阻肺急性加重患者作为训练集。并收集2020年1月—2020年12月期间的慢阻肺急性加重患者作为验证集。通过单因素、Lasso回归分析及多因素Logistic分析到独立危险因素。用独立危险因素构建列线图预测模型,并通过受试者工作特征(Receiver operating characteristic,ROC)曲线下面积(The area under ROC curve,AUC)、校准曲线和决策曲线分析(decision curve analysis,DCA)对列线图进行多方面评估。结果使用糖皮质激素、抗生素联合使用、抗生素使用时间及低蛋白血症是慢阻肺急性加重患者继发肺部真菌感染的独立危险因素(均P<0.05)。所构建预测模型的训练集和验证集的AUC值分别为0.915[95%CI:0.891~0.940]和0.830[95%CI:0.790~0.871]。训练集和验证集中的校准曲线显示,列线图预测的真菌感染概率与实际情况相符。而DCA曲线结果表明该列线图预测模型具有较好的临床实用性。结论糖皮质激素、多种抗生素联合使用、抗生素使用时间过长及低蛋白血症是慢阻肺急性加重患者继发肺部真菌感染的独立危险因素。本研究构建的慢阻肺急性加重患者继发肺部真菌感染的临床预测模型,具有较强的预测价值及实用性。

关键词：慢性阻塞性肺疾病急性加重期肺部真菌感染列线图预测模型

Zinc Absorption from Representative Diet in a Chinese Elderly Population Using Stable Isotope Technique

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Biomedical and Environmental Sciences 2017年第6期30卷 391-397页

作者： LI ya Jie LI Min LIU Xiao Bing REN Tong Xiang LI Wei Dong yaNG Chun WU Meng yaNG lin Li MA Yu Xia WANG Jun PIAO Jian Hua yaNG Li Chen yaNG Xiao Guang Key Laboratory of Trace Element Nutrition National Health and Family Planning Commission of China Department of Trace Element Nutrition National Institute for Nutrition and Health Chinese Center for Disease Control and Prevention Beijing 100050 China National Institute of Metrology Beijing 100029 China Department of Nutrition and Food Hygiene Capital University of Medical Sciences Beijing 100069 China HeBei Medical University Shijiazhuang 050017 Hebei China

Objective To determine the dietary zinc absorption in a Chinese elderly population and provide the basic data for the setting of zinc （Zn） recommended nutrient intakes （RNI） for Chinese elderly people. Methods A total of 24 elderly people were recruited for this study and were administered oral doses of 3 mg 67Zn and 1.2 mg dysprosium on the fourth day. The primary macronutrients, energy, and phytic acid in the representative diet were examined based on the Chinese National Standard Methods. Fecal samples were collected during the experimenta and dysprosium content. period and analyzed for zinc content, 67Zn isotope ratio, Results The mean （± SD） zinc intake from the representative Chinese diet was 10.6 ± 1.5 mg/d. The phytic acid-to-zinc molar ratio in the diet was 6.4. The absorption rate of 67Zn was 27.9% ±9.2%. The RNI of zinc, which were calculated by the absorption rate in elderly men and women, were 10.4 and 9.2 mg/d, respectively. Conclusion This study got the dietary Zn absorption in a Chinese elderly population. We found that Zn absorption was higher in elderly men than in elderly women. The current RNI in elderly female is lower than our finding, which indicates that more attention is needed regarding elderly females＇ zinc status and health.

关键词： Zinc absorption Chinese elderly people Stable isotope technique

高校实验室安全事故人为因素及其影响路径分析

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实验室研究与探索 2024年第7期43卷 264-268页

作者：王维马麟北京工业大学材料科学与工程学院北京100124 北京工业大学新型功能材料教育部重点实验室北京100124

为了通过人因分析与分类系统模型从组织影响、不安全的监督、不安全行为的前提条件和不安全行为4个层面探究高校实验室安全事故的关键人因及其影响路径。对90起高校实验室事故(2000—2022年)进行统计分析,并运用卡方检验和让步比探讨了... 详细信息

为了通过人因分析与分类系统模型从组织影响、不安全的监督、不安全行为的前提条件和不安全行为4个层面探究高校实验室安全事故的关键人因及其影响路径。对90起高校实验室事故(2000—2022年)进行统计分析,并运用卡方检验和让步比探讨了产生不安全行为的原因和各层次间的内在联系。结果表明:组织过程简单、监督不充分、个人因素、技能失误和习惯性违规是导致高校实验室事故发生的重要因素;模型中高低层级的人为因素间存在显著的因果关系,且主要的实验室事故致因路径共有16条。根据各层级事故致因形式,剖析了针对性的高校实验室事故的影响过程。

关键词：高校实验室事故人因分析与分类系统(HFACS) 卡方检验让步比

Effect of moxibustion on T lymphocyte subsets in peripheral blood of rats with gastric cancer

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Journal of Acupuncture and Tuina Science 2019年第3期17卷 161-167页

作者： Tan Jing yang Ren-da Zhao Huan Peng Zhuo-jun Ouyang Li-zhi lin ya-ping yang yan-ping Hunan University of Chinese Medicine Changsha 410208China 不详

Objective:To observe the effect of moxibustion on T lymphocyte subsets in peripheral blood of rats with gastric cancer. Methods:Sixty healthy Sprague-Dawley (SD) rats were adaptively fed for one week. By the random number table method, 10 rats were randomly selected as a blank group, and 12 rats were randomly selected to simulate the tumor transplantation process;after transplantation, 10 rats were randomly selected as a sham operation group. The remaining 38 rats were used to prepare gastric cancer models by gastric transplantation of the Walker-256 tumor tissue;8 rats were randomly selected to verify the successful modeling after 7 d;the remaining 30 rats were randomly divided into a model group, a moxibustion group and an infrared group by the random number table method, with 10 rats in each group. From the first day of enrollment, the rats in the moxibustion group received mild moxibustion at Zhongwan (CV 12), Guanyuan (CV 4) and bilateral Zusanli (ST 36) (the first group) and bilateral Pishu (BL 20) and Weishu (BL 21) (the second group), and the two groups of acupoints were alternated every other day. The rats in the infrared group received infrared radiation on the stomach area and the area on the back between the T12-T13 spinous processes, the two areas were alternated every other day. Rats in the moxibustion group and the infrared group were treated for 20 min each time, once a day for 21 d. Rats in the blank group, the sham operation group, and the model group were simultaneously grasped and fixed, and no other treatment was performed. After 21 d of intervention, the rats in each group were fasted for 12 h, and blood was collected from the orbits. The numbers of CD3+, CD3+CD4+, CD3+CD8+ T lymphocytes in peripheral blood were determined by flow cytometry, and the ratio of CD3+CD4+/CD3+CD8+ was calculated. The rats were sacrificed and the thymus was dissected under sterile conditions to calculate the thymus index. Results:Compared with the blank group, the th

关键词： Acupuncture-moxibustion Therapy Moxibustion Therapy Moxa Stick Moxibustion Neoplasms Stomach T-lymphocytes Immunity, Cellular Rats

社区老年人互助养老需求与双向社会支持的现状及关系研究

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护理学杂志 2024年第12期39卷 103-106页

作者：陈醒彭琳长沙医学院护理学院湖南长沙410219

目的了解社区老年人互助养老需求与双向社会支持现状,并分析其相关关系,为针对性干预提供参考。方法于2022年6-9月方便抽取湖南省3个社区256名居家老年人,采用一般资料问卷、社区居家互助养老需求量表及简版双向社会支持量表进行调查。... 详细信息

目的了解社区老年人互助养老需求与双向社会支持现状,并分析其相关关系,为针对性干预提供参考。方法于2022年6-9月方便抽取湖南省3个社区256名居家老年人,采用一般资料问卷、社区居家互助养老需求量表及简版双向社会支持量表进行调查。结果社区居家互助养老需求总分为(34.86±7.35)分;双向社会支持总分为(38.64±4.08)分。社区居家互助养老需求总分与双向社会支持总分呈正相关(r=0.398,P<0.05)。多元线性回归分析结果显示,社区老年人的居住方式、锻炼情况、所患慢性病数量及双向社会支持得分是互助养老需求的主要影响因素(均P<0.05),共能解释39.8%的变异。结论社区老年人互助养老需求及双向社会支持处于中等偏低水平,互助养老需求受多种因素影响。应针对老年人个体特征及需求予以干预,以促进互助养老模式开展。

关键词：社区老年人互助养老需求双向社会支持医疗保健人文关怀娱乐学习生活服务

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母猪能量和蛋白质营养研究进展

动物营养学报 2022年第10期34卷 6167-6177页

作者：吴德林燕王亚四川农业大学动物营养研究所成都611130 动物抗病营养教育部重点实验室成都611130

能量和蛋白质是母猪饲粮中最重要的两大营养素,占饲粮组成和饲料成本的90%以上,其水平和来源显著影响母猪繁殖性能。能量水平高低不仅影响母猪单胎繁殖成绩,还影响母猪繁殖寿命;不同能量来源以及淀粉、脂肪和纤维的比例对母猪情期启动... 详细信息

能量和蛋白质是母猪饲粮中最重要的两大营养素,占饲粮组成和饲料成本的90%以上,其水平和来源显著影响母猪繁殖性能。能量水平高低不仅影响母猪单胎繁殖成绩,还影响母猪繁殖寿命;不同能量来源以及淀粉、脂肪和纤维的比例对母猪情期启动、胚胎发育和泌乳性能的作用效应不同,三者协同可调控母猪繁殖成绩。蛋白质水平同样显著影响母猪的繁殖成绩,蛋白质来源主要与氨基酸水平密切相关,氨基酸水平对胎儿发育和泌乳性能的影响一直是母猪营养研究与应用的热点。本文通过对饲粮能量和蛋白质营养在母猪上的研究成果进行综述,为母猪饲粮的精准配制和增效降本提供重要依据。

关键词：母猪能量蛋白质

Prevalence of chronic kidney disease and prediabetes and associated risk factors： a community-based screening in Zhuhai, Southern China

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Chinese Medical Journal 2013年第7期126卷 1213-1219页

作者： GU Dong-feng SHI yan-lin CHEN You-mingt LIU Hong-mei DING ya-nan LIU Xin-yu LI Yong-qiang SHAO Xiao-fei LIANG yan CHEN Ying-shan YUAN Zhi-yong ZOU He-qun Department of Nephrology Third Affiliated Hospital of Southern Medical University Guangzhou Guangdong 510630 China Clinical Laboratory Third Affiliated Hospital of Southern Medical University Guangzhou Guangdong 510630 China Ultrasonic Angi-ology Laboratory Third Affiliated Hospital of Southern Medical University Guangzhou Guangdong 510630 China Department of Cardiovascular Medicine Third Affiliated Hospital of Southern Medical University Guangzhou Guangdong 510630 China Department of Nephrology First Affiliated Hospital of WenzhouMedical College Wenzhou Zhejiang 325035 China Department of Nephrology Zhangzhou Municipal Hospital ot Fuiian Province Zhangzhou Ft~iian 363000 China Department of Nephrology Zhuhai Wanzai Hospital Zhuhai Guangdong 519000 China

Background The prevalence of chronic kidney disease （CKD） and prediabetes has increased in China, and at different rates in different locations. Therefore a community-based screening research was conducted in order to determine the prevalence of CKD and prediabetes, and to analyze associated risk factors of CKD and prediabetes in a city of Southern *** A total of 7801 community residents aged 18 year and older from 6 communities were screened by a stratified random cluster sampling method. An estimated glomerular filtration rate （eGFR）, albuminuria, fasting plasma glucose （FPG）, and homeostatic model assessment of insulin resistance （HOMA-IR） were measured. Age-standardized prevalence was calculated by the direct method with the use of data on the population distribution in China in 2006. Multivariate logistic analysis was used to analyze the risk factors of CKD and ＂prediabetes, and association of insulin resistance （IR） with CKD and prediabetes was *** The age-standardized prevalence of CKD was 12.5%, eGFR 〈60 ***^-1.1.73 m2 was 2.7% and ACR （albumin to creatinine ratio）〉30 mg/g was 10.3%. The age-standardized prevalence of prediabetes was 12.1%. Logistic regression suggests that IR was a common independent risk factor of CKD and prediabetes. Further analysis show that HOMA-IR was increased with the aggravation of kidney injury and FPG. Conclusion CKD and prediabetes have become a major public health problem in Zhuhai, Southern China; insulin resistance may be an important risk factor.

关键词： chronic kidney disease," prediabetes homeostatic model assessment of insulin resistance," prevalence,"community-based screening

杂交兰ChCAO基因克隆及其在叶艺品系中的表达特征

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西北植物学报 2024年第3期44卷 421-429页

作者：林榕燕孔兰吴建设林兵钟淮钦福建省特色花卉工程技术研究中心福州350013

【目的】叶绿素酸酯a加氧酶(CAO)是叶绿素b形成过程中的关键酶,对杂交兰CAO基因进行克隆及表达特征分析可为探究其在杂交兰叶艺形成中的调控作用奠定基础。【方法】以杂交兰‘紫妍氏’(K21)及其叶艺品系‘中透紫妍氏’(K21-3)为试验材料... 详细信息

【目的】叶绿素酸酯a加氧酶(CAO)是叶绿素b形成过程中的关键酶,对杂交兰CAO基因进行克隆及表达特征分析可为探究其在杂交兰叶艺形成中的调控作用奠定基础。【方法】以杂交兰‘紫妍氏’(K21)及其叶艺品系‘中透紫妍氏’(K21-3)为试验材料,用RT-PCR和RACE技术从叶片中克隆获得ChCAO基因,对ChCAO进行结构特征、理化性质、序列比对以及系统进化关系等分析;用qRT-PCR法对ChCAO在不同组织及叶艺品系叶片中的表达特性进行分析;并用VIGS技术对ChCAO进行沉默表达。【结果】ChCAO基因编码区长1608 bp,编码535个氨基酸,ChCAO与墨兰CAO亲缘关系最近,并与其他兰科植物CAO聚为一类。qRT-PCR结果显示,ChCAO基因表达具有组织特异性,在叶中相对表达量最高,根中相对表达量最低;此外,ChCAO在K21绿叶和K21-3绿叶区域叶片中的相对表达量显著高于K21-3叶艺区域叶片中的相对表达量。构建该基因的VIGS沉默载体转化烟草,发现沉默ChCAO后烟草叶片呈黄化状态,叶片中的叶绿素含量及ChCAO基因的相对表达量也显著降低。【结论】克隆获得了杂交兰ChCAO基因,并对其功能进行了初步鉴定,为进一步研究杂交兰叶艺形成机理提供重要依据。

关键词：杂交兰 ChCAO基因基因克隆表达分析