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Rht基因对春小麦和硬粒小麦离体单雄生殖的影响

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华中农业大学学报 2000年第3期19卷 219-222页

作者： Thac.,ov Djat.,TI 俄罗斯萨拉托夫州立农业大学生物工程与植物遗传育种系俄罗斯萨拉托夫州东南地区农科所

研究了离体条件下Ｒｈｔ基因对单雄生殖的影响。选用Ｒｈｔ基因的不同等位基因品系作为抗体材料，包括以砚小麦为背景的Ｒｈｔ１，Ｒｈｔ３、Ｒｈｔ１４及以硬粒小麦Ｃｈａｒｋｏｖｓｋａｙａ４６为背景的Ｒｈｔ１４和Ｒｈｔ１。结果... 详细信息

研究了离体条件下Ｒｈｔ基因对单雄生殖的影响。选用Ｒｈｔ基因的不同等位基因品系作为抗体材料，包括以砚小麦为背景的Ｒｈｔ１，Ｒｈｔ３、Ｒｈｔ１４及以硬粒小麦Ｃｈａｒｋｏｖｓｋａｙａ４６为背景的Ｒｈｔ１４和Ｒｈｔ１。结果表明：不同的Ｒｈｔ遗传体系对愈伤诱导频率和植株再生率的影响不同。在砚小麦中，显性Ｒｈｔ５基因对单倍体产生的所有阶段都有明显的正向效应。而在硬粒小麦中。

关键词：小麦离体单雄生殖 Rht基因愈伤组织植株再生

用于伏尔加河地区矮秆春小麦育种的Rht基因的研究和选择

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华中农业大学学报 2000年第3期19卷 209-212页

作者： Loba.,YV Kurp.,ov 俄罗斯萨拉托夫州立农业大学生物工程与植物遗传育种系俄罗斯萨拉托夫州东南地区农科所

对小麦品系Ｓａｒａｔｏｖｓｋａｙａ２９的不同Ｒｈｔ等位的一套近等基因系进行了田间试验，以测定６个矮杆基因的育种值。在不灌溉、灌溉并施用不同剂量的肥料（Ｎ１２０，Ｐ１００，Ｋ６０和Ｎ１８０，Ｐ１６０，Ｋ１００）三种条件... 详细信息

对小麦品系Ｓａｒａｔｏｖｓｋａｙａ２９的不同Ｒｈｔ等位的一套近等基因系进行了田间试验，以测定６个矮杆基因的育种值。在不灌溉、灌溉并施用不同剂量的肥料（Ｎ１２０，Ｐ１００，Ｋ６０和Ｎ１８０，Ｐ１６０，Ｋ１００）三种条件下测定Ｒｈｔ基因对株高、产量及产量构成因子、品质的影响。结果表明，Ｒｈｔ基因可以用于伏尔加河地区条件下魏杆小麦品系的选育。

关键词：春小麦产量品质矮杆基因育种值

The twin-beginnings of CovID-19 in Asia and Europe—one prevails quickly

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National Science Review 2022年第4期9卷 51-59页

作者： Yongsen Ruan Haijun Wen Mei Hou Ziwen He Xuemei Lu Yongbiao Xue Xionglei He ya-Ping Zhang Chung-I Wu State Key Laboratory of Biocontrol School of Life SciencesSouthern Marine Science and Engineering Guangdong Laboratory (Zhuhai)Sun Yat-sen University Department of Ecology and Evolution University of Chicago State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of Sciences Beijing Institute of Genomics Chinese Academy of Sciencesand China National Centre for Bioinformation

In the spread of SARS-C ov-2,there have been multiple waves of replacement between strains,each of which having a distinct set of *** first wave is a group of four mutations(C241 T,C3037 T,C14408 T and A23403 G [this being the amino acid change D614 G];all designated 0 to 1 below).This DG(D614 G) group,fixed at the start of the pandemic,is the foundation of all subsequent waves of ***,the DG group is absent in early Asian samples but present(and likely common) in Europe from the *** data show that the high fitness of DG1111 requires the synergistic effect of all four ***,the European strains would have had no time to evolve the four DG mutations(0 to1),had they come directly from the early Asian DG0000 *** likely,the European DG1111 strain had acquired the highly adaptive DG mutations in pre-pandemic Europe and had been spreading in parallel with the Asian *** recent reports further support this twin-beginning *** was a period of two-way spread between Asia and Europe but,by May 2020,the European strains had supplanted the Asian strains *** large-scale replacement of one set of mutations for another has since been replayed many times as CovID-19 progresses.

关键词： SARS-Cov-2 CovID-19 D614G twin-beginnings fitness advantage

Reproducibility of optical quality parameters measured at objective and subjective best focuses in a doublepass system

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International Journal of Ophthalmology(English edition) 2015年第5期8卷 1043-1050页

作者： Ai-Lian Hu Li-ya Qiao Ye Zhang Xiao-Gu Cai Lei Li Xiu-Hua Wan Beijing Institute of Ophthalmology Beijing Tongren Eye CenterBeijing Tongren HospitalCapital Medical UniversityBeijing Ophthalmology & Vision Science Key Lab

AIMTo evaluate intra-session repeatability and reproducibility of optical quality parameters measured at objective and subjective best focuses in a double-pass *** Chinese healthy adults (19 to 40 years old) meeting our inclusion criterion were enrolled in the study. After a basic eye examination, two methods of optical quality measurement, based on subjective and objective best focuses were performed using the Optical Quality Analysis System (OQAS) with an artificial pupil diameter of 4.0 *** each method, three consecutive measurements of the following parameters: the modulation transfer function cutoff frequency (MTFcutoff), the Strehl2D ratio, the OQAS values (ovs) at contrasts of 100%, 20%, 9% and the objective scatter index (OSI) were performed by an experienced examiner. The repeatability of each method was evaluated by the repeatability limit (RL) and the coefficient of repeatability (COR). Reproducibility of the two methods was evaluated by intra-class correlation coefficient (ICC) and the 95% limits of agreement (Bland and Altman analysis). Thirty subjects, seven females and twenty three males, of whom 15 right eyes and 15 left eyes were selected randomly for recruitment in the study. The RLs (percentage) for the six parameters measured at objective focus and subjective focus ranged from 8.44% to 15.13% and 10.85% to 16.26%, respectively. The CORs for the two measurement methods ranged from 8.27% to 14.83% and 10.63% to 15.93%, respectively. With regard to reproducibility, the ICCs for the six parameters of OQAS ranged from 0.024 to 0.276. The 95% limits of agreement obtained for the six parameters (in comparison of the two methods) ranged from -0.57 to 42.18 (MTFcutoff), -0.01 to 0.23 (Strehl2D ratio), -0.02 to 1.40 (ov100%), -0.10 to 1.75 (ov20%), -0.14 to 1.80 (ov9%) and -1.46 to 0.18 (OSI).CONCLUSIONMeasurements provided by OQAS with either method sh

关键词： optical quality double-pass system best focus repeatability reproducibility

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ov式与汉语的一种指称性语法手段

汉语学习 2007年第5期 25-32页

作者：肖娅曼四川大学中文系四川成都610064

汉语中存在着像"冰雕"一类的ov逆序复合名词,它们尚未引起广泛重视。ov逆序构词法是一种与句法相对立的复合构词法,这种ov逆序构词法上古即已出现,并发展为一般的专门表示指称的ov逆序法,"祖国颂"和"环境污染"等指称结构即是它的产物。... 详细信息

汉语中存在着像"冰雕"一类的ov逆序复合名词,它们尚未引起广泛重视。ov逆序构词法是一种与句法相对立的复合构词法,这种ov逆序构词法上古即已出现,并发展为一般的专门表示指称的ov逆序法,"祖国颂"和"环境污染"等指称结构即是它的产物。如果汉语复合词的词法和句法的一致性是因复合词大多由句法结构词汇化而来,那么ov逆序复合法和ov逆序法将使我们注意到,汉语存在着由词法向句法的反向发展。

关键词： ov复合词 ov逆序构词法 ov结构

New insights into the mechanism of reactive adsorption desulfurization on Ni/ZnO catalysts:Theoretical evidence showing the existence of interfacial sulfur transfer pathway and the essential role of hydrogen

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Petroleum Science 2023年第5期20卷 3240-3250页

作者： Hou-Yu Zhu Nai-You Shi Dong-Yuan Liu Rui Li Jing-Gang Yu Qi-Tang Ma Tu-ya Li Hao Ren Yuan Pan Yun-Qi Liu Wen-Yue Guo School of Materials Science and Engineering China University of Petroleum(East China)Qingdao266580ShandongChina State Key Laboratory of Heavy Oil Processing College of Chemistry and Chemical EngineeringChina University of Petroleum(East China)Qingdao266580ShandongChina

As well known in the petroleum industry and academia,Ni/ZnO catalysts have excellent desulfurization ***,the sulfur transfer mechanism of reactive adsorption desulfurization(RADS)that occurs on Ni/ZnO catalysts remains ***,a periodic Ni nanorod supported on ZnO slab was built to represent the Ni/ZnO system,and density functional theory calculations were performed to study the sulfur transfer process and the role of H_(2)within the *** results elucidate that the direct solid-state diffusion of S from Ni to interfacial oxygen vacancies(ov)is more favorable than the hydrogenation of S to SH/H_(2)S on Ni and the subsequent H_(2)S desorption,and accordingly,H_(2)O is produced on Ni rather than on *** initio thermodynamics analysis shows that the hydrogen atmosphere applied in preparing Ni/ZnO catalysts greatly promotes the O_(v)formation on ZnO surface,which accounts for the presence of interfacial O_(v)in freshly prepared *** RADS condition,hydrogenation of interfacial O atoms to form O-H groups facilitates the reverse spillover of these lattice O atoms from ZnO to Ni,accompanied with the interfacial O_(v)*** contrast to the classic S transfer mechanism via H_(2)S,the present work clearly demonstrates that the interfacial S transfer is a feasible reaction pathway in the RADS *** importantly,the existence of interfacial O_(v)is an essential prerequisite for this interfacial S diffusion,and H_(2)plays a key role in facilitating the O_(v)formation.

关键词： Reactive adsorption desulfurization Ni/ZnO Interface Sulfur transfer Density functional theory

叶酸受体阳性肿瘤细胞对Folate-PGA偶联酶的特异性结合

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中国药理学通报 2007年第6期23卷 746-750页

作者：张奇张友九杨科亚项光亚华中科技大学同济医学院药学院湖北武汉430030 苏州大学放射医学与公共卫生学院江苏苏州215007

目的考察叶酸受体阳性肿瘤细胞对叶酸偶联的青霉素G酰化酶(Folate-PGA)的特异性结合。方法采用Iodo-gen法标记的125I-Folate-PGA测定叶酸受体表达阳性FR(+)HeLa与SKov3细胞以及叶酸受体表达阴性FR(-)A549细胞于4℃对Folate-PGA偶联酶的... 详细信息

目的考察叶酸受体阳性肿瘤细胞对叶酸偶联的青霉素G酰化酶(Folate-PGA)的特异性结合。方法采用Iodo-gen法标记的125I-Folate-PGA测定叶酸受体表达阳性FR(+)HeLa与SKov3细胞以及叶酸受体表达阴性FR(-)A549细胞于4℃对Folate-PGA偶联酶的结合。结果125I-Folate-PGA对FR(-)A549无特异性结合;与HeLa和SK-ov3细胞的特异性结合的Kd分别为0.11nmol·L-1和0.25nmol·L-1。结论叶酸偶联的PGA酶对FR(+)肿瘤细胞具有较好的亲和力和靶向性,可用于叶酸导向的酶前体药物疗法的进一步研究。

关键词：叶酸靶向特异性结合青霉素G酰化酶偶联

衍生化毛细管气相色谱法同时测定无糖食品中的多种糖醇类甜味剂

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分析化学 2013年第6期41卷 911-916页

作者：张雅珩周围李斌甘肃出入境检验检疫局检验检疫综合技术中心

建立了一种同时测定无糖食品中多种糖醇类甜味剂的气相色谱定量分析方法。样品中的糖醇经水提取,净化、脱水后,在吡啶-乙酸酐(1∶1,V/V)存在条件下,于90℃反应20 min,生成的糖醇乙酸酯衍生物用ov-101毛细管色谱柱(50 m×0.25 mm×0.33... 详细信息

建立了一种同时测定无糖食品中多种糖醇类甜味剂的气相色谱定量分析方法。样品中的糖醇经水提取,净化、脱水后,在吡啶-乙酸酐(1∶1,V/V)存在条件下,于90℃反应20 min,生成的糖醇乙酸酯衍生物用ov-101毛细管色谱柱(50 m×0.25 mm×0.33μm)分离,氢火焰离子化检测器(FID)进行测定及气相色谱-质谱结构鉴定。在优化的色谱分析条件下,实现了赤藓糖醇、木糖醇、甘露糖醇、山梨糖醇和麦芽糖醇衍生物的理想分离,并排除了常见糖类对糖醇测定的干扰。本方法在0.01～1.6 mg/mL范围内,对糖醇浓度与其衍生物峰面积进行线性回归分析,其相关系数在0.9995～0.9999之间。平均回收率为74.6%～113.4%,相对标准偏差在2.4%～8.6%之间。本方法操作简便、快速、可靠,检测限和加标回收率均能满足无糖食品中糖醇的定量分析要求。

关键词：气相色谱糖醇衍生化无糖食品

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Chinese Medical Journal 2020年第19期133卷 2308-2314页

作者： Xi-Fang Zhang Li-ya Qiao Xiao-Gu Cai Xiao-Xia Li Jia-Xuan Tan Zheng Guan Ye Zhang Kai Cao Ning-Li Wang Beijing Tongren Eye Center Beijing Tongren HospitalCapital Medical UniversityBeijing Institute of OphthalmologyBeijing Ophthalmology and Visual Sciences Key LaboratoryBeijing 100730China.

Background:In recent years,visual quality has been extensively investigated in various *** this community-based population study,we analyzed the effects of aging,refraction,and Lens Opacification Classification System III(LOCSIII)score on retinal imaging quality in healthy Chinese ***:This cross-sectional study was conducted on sub-group subjects from The Handan Eye Study between October 2012 and January *** subjects over 30-years-old with logarithm of the minimal angle of resolution(logMAR)best-corrected visual acuity(BCVA)less than 0 were *** image quality was measured by optical quality analysis system(OQAS)and recorded as modulation transfer function cutoff frequency(MTFcutoff),OQAS value(ov)100%,ov20%,ov9%,Strehl ratio(SR),and objective scatter index(OSI).The correlation between age,spherical equivalent refraction(SE),LOCSIII score,and optical quality parameters were investigated by multivariate ***:Among 1108 verified subjects,690 subjects(1380 eyes)met the inclusion *** age ranged from 30 to 76 years,SE ranged from-4.75 to 2.75 *** were divided into five age groups(30–39,40–49,50–59,60–69,and≥70 years)for further *** multivariate analysis by mixed-effect linear model,SR(t=-3.03,P=0.002),ov20%(t=-2.39,P=0.017),and ov9%(t=-3.16,P=0.001)significantly decreased with the increasing age,whereas logMAR BCVA(t=4.42,Pov20%(t=2.31,P=0.02),and ov9%(t=2.79,P=0.005)significantly elevated,and OSI(t=-3.38,Pov100%(t=-2.78,P=0.005),ov20%(t=-2.60,P=0.009),and ov9%(t=-2.05,P=0.04).As posterior sub capsular opacity score increased,MTFcutoff(t=-2.40,P=0.02)and ov100%(t=-2.40,P=0.01)significantly decreased,while OSI(t=7.56,P<0.00

关键词： Cross-sectional study Prospective study Vision Aging Refractive errors Cataract