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In situ observation of surface structures of cardiovascular endothelial cells with atomic force microscope

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Journal of Geriatric Cardiology 2009年第3期6卷 178-181页

作者： Tong Yin Jin Luo ya-min ma Xiao-Long Ji Yu-Sheng Zhao Shi-Wen Wang Institute of Geriatric Cardiology Department of Cardiovascular Surgery General Hospital of PLA Beijing 100853 China Institute of Nanomedicine General Hospital o fArmed Police Forces Beo＇ing 100039 China

Objective To observe the surface structures of cardiovascular endothelial cells in situ with atomic force microscope （AFM）. Methods Fresh aorta and aortic valve were dissected from 10 healthy male New Zealand white rabbits. Before fixed in 1% formaldehyde, the fresh tissues were washed in the buffer phosphate solution. Under general microscope, the fixed aorta or valve was spread on the double side stick tape which had already been stuck on the glass slide. The intima of aorta or the aorta side of valve was towards upside, Then the specimen was dried under 37 degrees centigrade in an attemperator and was washed with pure water. After dried again, the specimen was loaded on the platform ofNanoScope Ⅲa AFM and was scanned in tapping mode with the scanning speed of 0.5 HZ. Results The surface structures of endothelial cell on the fixed and dried tissue could be observed clearly in situ with AFM. aortic endothelial cells were large, branched and arranged sparsely and parallel to the direction of blood flow, whereas endothelial cells on aorta valve surface were small, less branched and arranged intensively and vertical to the direction of blood flow. When the scanning range was dwindled, granular ultra-structures could be observed on the surface of endothelial cells, and, as the scanning range was dwindled further, fissure and convolution could be seen on the surface of granules from aortic endothelial cells. Centre cavity and surrounding swelling volcano-like structure could be seen on the surface of granules from endothelial cells of aortic valve. Conclusions It＇s feasible to observe the surface ultra-structures of cardiovascular endothelial cells in situ with AFM and morphological information provided by AFM might be of clinical value in future histopathological diagnosis（JGeriatr Cardiol2009; 6：178-181）.

关键词： atomic force microscope endothelial cell

AFM OBSERVATION OF NORmaL HUmaN ERYTHROCYTES PROCESSED BY SEVERAL SOLUTIONS

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AFM OBSERVATION OF NORMAL HUMAN ERYTHROCYTES PROCESSED BY SE...

Small extracellular vesicles derived from human induced pluripotent stem cell-differentiated neural progenitor cells mitigate retinal ganglion cell degeneration in a mouse model of optic nerve injury

Asia Conference on Nanoscience and Nanotechnology Abstracts Book

作者： ya-min ma XIAO-LONG JI YIN TONG minG-SHI SHEN Institute of Geratology General Hospital of PLA Beijing 100853 China Institute ofnanomedicine the General Hospital of Chinese People’s Armed Police Forces Beijing 100039 China

＜正＞ The application of atomic force microscopy （AFM） in clinical diagnosis is far from being developed. Suitable solutions for the observing of ervthrocyte by AFM are necessary. In this study, following solutions, 1% formaldehyde, citrate buffer, 5%dextrose and TAE, were found to keep human erythrocytes in normal shape with few particles. Processed by these solutions, fine structures of human erythrocyte’s membrane were obtained. The results will offer meaningful data for clinical diagnosis of blood by AFM.

关键词： Atomic Force Microscopy solution erythrocyte fine structures

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Neural Regeneration Research 2025年第2期20卷 587-597页

作者： Tong Li Hui-min Xing Hai-Dong Qian Qiao Gao Sheng-Lan Xu Hua ma Zai-Long Chi State Key Laboratory of Ophthalmology Optometry and Visual ScienceEye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina National Clinical Research Center for Ocular Diseases Eye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina

Several studies have found that transplantation of neural progenitor cells(NPCs)promotes the survival of injured ***,a poor integration rate and high risk of tumorigenicity after cell transplantation limits their clinical *** extracellular vesicles(sEVs)contain bioactive molecules for neuronal protection and *** studies have shown that stem/progenitor cell-derived sEVs can promote neuronal survival and recovery of neurological function in neurodegenerative eye diseases and other eye *** this study,we intravitreally transplanted sEVs derived from human induced pluripotent stem cells(hiPSCs)and hiPSCs-differentiated NPCs(hiPSC-NPC)in a mouse model of optic nerve *** results show that these intravitreally injected sEVs were ingested by retinal cells,especially those localized in the ganglion cell *** with hiPSC-NPC-derived sEVs mitigated optic nerve crush-induced retinal ganglion cell degeneration,and regulated the retinal microenvironment by inhibiting excessive activation of *** analysis further revealed that hiPSC-NPC derived sEVs transported neuroprotective and anti-inflammatory miRNA cargos to target cells,which had protective effects on RGCs after optic nerve *** findings suggest that sEVs derived from hiPSC-NPC are a promising cell-free therapeutic strategy for optic neuropathy.

关键词： exosome miRNA neural progenitor cell neurodegeneration neuroinflammation neuroprotection optic nerve crush optic neuropathy retinal ganglion cell small extracellular vesicles

对正常胃、胃溃疡及胃癌组织中弹力纤维分布规律的观察

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世界华人消化杂志 2005年第3期13卷 308-311页

作者：关维纪小龙徐薪马亚敏尹彤申明识中国人民解放军总医院病理科北京市100853 中国武警总医院病理科北京市100039 中国人民解放军总医院老年病研究所北京市100853 中国人民解放军总医院老年心学管病研究所北京市100853

目的:通过对正常胃组织、胃溃疡及胃癌所在胃壁组织中弹力纤维(EF)的观察,总结EF在其中的分布规律及异同并分析EF增多的原因. 方法:正常胃标本17例,胃溃疡标本7例,胃癌标本40例.进行常规取材、固定、包埋、石蜡切片、HE 染色和改良的Wei... 详细信息

目的:通过对正常胃组织、胃溃疡及胃癌所在胃壁组织中弹力纤维(EF)的观察,总结EF在其中的分布规律及异同并分析EF增多的原因. 方法:正常胃标本17例,胃溃疡标本7例,胃癌标本40例.进行常规取材、固定、包埋、石蜡切片、HE 染色和改良的Weigert染色,对染色结果进行观察、对比、分析. 结果:正常胃壁中EF呈规律性分布,主要位于黏膜肌层和肌层临近黏膜下层侧,纤维毛细状,数量少,分布均匀. 胃溃疡EF只在溃疡旁的黏膜肌层有显著增多,纤维增粗, 着色深,排列紊乱,当溃疡深达肌层时,其中EF增多不明显.胃癌EF在平滑肌细胞和成纤维细胞分布区域均有不同程度增多,纤维增粗,着色深,排列紊乱.胃癌组与渍疡组增多的EF数量上存在显著差异(P=0.0 042, P<0.05).胃癌组织中小血管内皮细胞增生,而胃溃疡组中未见血管内皮细胞增生现象. 结论:EF在正常胃组织、胃溃疡及胃癌周围组织中均呈规律性分布,且三者存在显著不同.胃溃疡组织EF增多可能与慢性炎症的刺激有关;胃癌周围组织中EF的增多除慢性炎症外还应该存在其他刺激因素,癌细胞还可能向外发出某种刺激因子,这些因子不仅作用于癌旁组织,还可以渗透到癌组织周围至胃壁浆膜层,作用于沿途的平滑肌细胞、成纤维细胞,使其生成EF 的能力增强,从而使其中的EF增多、增粗.癌组织还可能刺激周围组织,直接通过基因的调节使胃壁中能生成EF的细胞功能激活并增强,EF生成增多.胃壁中EF增多是一个慢性过程.胃癌组织中小血管内皮细胞增生是肿瘤相关性病变.

关键词：胃溃疡正常胃癌组织胃壁血管内皮细胞增生周围组织观察 EF 中小排列

东祁连山退化高寒草地土壤细菌群落与土壤环境因子间的相互关系

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应用生态学报 2018年第11期29卷 3793-3801页

作者：李海云姚拓张建贵高亚敏马亚春路晓雯张慧荣杨晓蕾甘肃农业大学草业学院草业生态系统教育部重点实验室兰州730070

为探究东祁连山不同退化高寒草地细菌群落分布特征与土壤环境因子间的相互关系,采用高通量测序技术对轻度、中度和重度退化草地的土壤细菌群落结构变化及其多样性进行分析,运用CANOCO 4.5软件对土壤细菌群落与土壤环境因子间关系进行冗... 详细信息

为探究东祁连山不同退化高寒草地细菌群落分布特征与土壤环境因子间的相互关系,采用高通量测序技术对轻度、中度和重度退化草地的土壤细菌群落结构变化及其多样性进行分析,运用CANOCO 4.5软件对土壤细菌群落与土壤环境因子间关系进行冗余分析(RDA).结果表明:不同退化高寒草地土壤理化性质间均差异显著,高通量测序共得到257125条有效序列,180826条优质序列,4790个OTUs.细菌群落Chao1指数依次为轻度>中度>重度;Shannon指数依次为轻度>重度>中度.系统发育分析表明,各样地土壤细菌类群分属于33个门,其中放线菌门、变形菌门和厚壁菌门是3种不同退化草地土壤中的优势类群.对不同退化草地土壤细菌各门所占比例分析发现,放线菌门、酸杆菌门和变形菌门随着退化程度加剧先减少后增加,厚壁菌门反之.RDA分析结果显示,细菌优势类群与蔗糖酶、纤维素酶和磷酸酶呈极显著相关,与pH、电导率、速效氮、速效钾呈显著相关.说明东祁连山不同退化高寒草地土壤细菌群落间差异明显,土壤环境因子是影响土壤细菌群落分布的重要因素.

关键词：东祁连山退化草地细菌群落结构高通量测序土壤环境因子冗余分析

De novo synthesis of atropisomeric benzofurans via Cu/SPDO complex catalyzed asymmetric formal [3 + 2] annulation

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Science China Chemistry 2025年第01期 174-180页

作者： Fan-Xiao Meng Xing-Yu Wang Ka Lu Ju-Song yang Fu-min Zhang Ai-Jun ma Bao Du Yong-Qiang Tu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical Engineering Lanzhou University School of Chemistry and Chemical Engineering Shanghai Jiao Tong University School of Biotechnology and Health Sciences Wuyi University

Herein, the de novo synthesis of atropisomeric benzofurans through the Cu/spirocyclic-pyrrolidine-oxazoline(SPDO) complex catalyze asymmetric formal [3 + 2] cycloaddition between quinones and ynamides is explored, producing a range of structurally diverse axially chiral benzofurans in satisfactory yields with good to excellent enantioselectivities. Furthermore, density functional theory calculations were applied to explain the possible reaction mechanism.

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Prevalence of Helicobacter pylori infection in Chinese military personnel:A cross-sectional,multicenter-based study

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World Journal of Gastroenterology 2025年第3期31卷 42-49页

作者： Han-Chen min Chun-yan Zhang Fang-Yu Wang Xiao-Hui Yu Shan-Hong Tang Hong-Wu Zhu ya-Gang Zhao Ji-Luo Liu Jian Wang Jing-Han Guo Xiao-Mei Zhang Yun-Sheng yang Chinese People’s Liberation Army Medical School Chinese People’s Liberation Army General HospitalBeijing 100853China Department of Gastroenterology and Hepatology The First Medical CenterChinese People’s Liberation Army General HospitalBeijing 100853China Sixth Healthcare Department The Second Medical CenterChinese People’s Liberation Army General HospitalBeijing 100853China Department of Gastroenterology The 940th Hospital of Joint Logistic Support Force of Chinese People’s Liberation ArmyLanzhou 730050Gansu ProvinceChina Department of Gastroenterology General Hospital of Western Theater CommandChengdu 610011Sichuan ProvinceChina Department of Gastroenterology General Hospital of Southern Theater CommandGuangzhou 510010Guangdong ProvinceChina Department of Epidemiology Naval Medical UniversityShanghai 200433China School of Medicine Nankai UniversityTianjin 300071China National Clinical Research Center for Geriatric Diseases Chinese People’s Liberation Army General HospitalBeijing 100853China

BACKGROUND Helicobacter pylori(***)infection is a prevalent disease encountered in military internal medicine and recognized as the main cause of dyspepsia,gastritis,and peptic ulcer,which are common diseases in military *** guidelines in China state all patients with evidence of active infection with *** are offered ***,the prevalence of *** infection and its regional distribution in the military population remain unclear,which hinders effective prevention and treatment *** the prevalence of *** infection in the military population will aid in the development of customized strategies to better manage this infectious *** To investigate the prevalence of *** infection in the Chinese military population in different geographic *** This multicenter,retrospective study included 22421 individuals from five tertiary hospitals located in north,east,southwest,and northwest cities of *** infection was identified using the urea breath test,which had been performed between January 2020 and December *** Of the 22421 military service members,7416(33.1%)were urea breath *** highest prevalence of *** was in the 30-39 years age group for military personnel,with an infection rate of 34.9%.The majority of infected subjects were younger than 40-years-old,accounting for 70.4%of the infected *** individuals serviced in Lanzhou and Chengdu showed a higher infection prevalence than those in Beijing,Nanjing,and Guangzhou,with prevalence rates of 44.3%,37.9%,29.0%,31.1%,and 32.3%,*** *** infection remains a common infectious disease among military personnel in China and has a relatively high prevalence rate in northwest China.

关键词： Helicobacter pylori Prevalence Military personnel Urea breath test Multicenter study

Energy Estimates on Existence of Extremals for Trudinger–Moser Inequalities

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Acta mathematica Sinica,English Series 2020年第7期36卷 829-841页

作者： ya min WANG School of mathematics Renmin University of ChinaBeijing 100872P.R.China

LetΩbe a smooth bounded domain in R2,W01,2(Ω)be the standard Sobolev *** the method of energy estimate developed by malchiodi–martinazzi(***.,16,893–908(2014)),mancini–martinazzi(*** Differential Equations,56,94(2017))and mancini–Thizy(*** Equations,266,1051–1072(2019)),we reprove the results of Carleson–Chang(***.,110,113–127(1986)),Flucher(***.,67,471–497(1992)),Li(Acta ***.,22,545–550(2006))and Su(***.,in press).Namely,for any real numberα≤1,the supremum■can be achieved by some function v∈W01,2(Ω)with║?v║22≤4π.

关键词： space. Existence 1,2

Variant selection of nanocrystalline a phase in metastableβTB8 titanium alloy via electric pulse treatment

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Rare Metals 2023年第8期42卷 2781-2794页

作者： Shan-Shan Wu ya yang Yuan-Biao Tan Song Xiang ya-Li Zhou min ma Fei Zhao Wei Shi Guizhou Key Laboratory of materials Mechanical Behavior and Microstructure College of Materials and MetallurgyGuizhou UniversityGuiyang 550025China College of materials Science and Engineering Hunan University of Science and TechnologyXiangtan 411201China

In this paper,the metastableβTB8 titanium alloy with nanocrystallineαphase is achieved by electric pulse *** morphology evolution and variant selection of nanocrystallineαphase in metastableβTB8titanium alloy were investigated by using scanning electron microscope(SEM),electron backscattered diffraction(EBSD)and transmission electron microscope(TEM)*** results indicated that the morphologies of the nanocrystallineαphase were mainly triangular clusters and needle-like at the pressure of 0 *** increasing pressure from 20 to 50 MPa,the volume fraction of needlelikeαphase decreased,and a large amount of V-shapedαphase formed in the interior ofβ*** on the EBSD data,the parentβphase was reconstructed by MTEX *** the interior of theβgrains,12 variants can form for the samples electric pulse treated at 0 and20 MPa,while only 3 and 6 variants can form for the samples electric pulse treated at 30 and 50 *** the grain boundary of theβgrains,one or more grain boundaryαvariants can be generated for the samples electric pulse treated at different pressures as long as one of the neighborβgrains follows the Burgers orientation relationship.

关键词： TB8 titanium alloy Parentβgrain reconstruction Morphology ofαphase Variant selection