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Symmetric alteration of four knots of B-spline and NURBS surfaces

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2006年第6期7卷 1084-1087页

作者： li ya-juan wang Guo-zhao Institute of Computer Graphics and Image Processing Department of Mathematics Zhejiang University Hangzhou 310027 China

Modifying the knots of a B-spline curve, the shape of the curve will be changed. In this paper, we present the effect of the symmetric alteration of four knots of the B-spline and the NURBS surfaces, i.e., symmetrical alteration of the knots of surface, the extended paths of points of the surface will converge to a point which should be expressed with several control points. This theory can be used in the constrained shape modification of B-spline and NURBS surfaces.

关键词： B-spline surface NURBS surface Knot modification Path

Relationship of PARG with PARP,VEGF and b-FGF in Colorectal Carcinoma

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Chinese Journal of Cancer Research 2009年第2期21卷 135-141页

作者： ling lin Jia li ya-lan wang Xiao lin Department of Pathology Molecular Medicine and Cancer Research Center Chongqing Medical UniversityChongqing 400016 China

Objective： To investigate the relationship of poly（ADP-ribose）glycohydrolase（PARG） with poly （ADP-ribose） polymerase（PARP）, vascular endothelial growth factor （VEGF） and basic fibroblast growth factor（b-FGF） in colorectal carcinoma（CRC）. Methods： Immunohistochemical analysis was used to detect PARG, PARP, VEGF and b-FGF in human colorectal carcinoma. Flow cytometry was used to detect PARG and PARP in murine CT26 cell line. Gallotannin （GLTN） was served as PARG inhibitor. Results： The individual positive rates of PARG, PARE VEGF and b-FGF were 55.81%（24/43）, 97.67%（42/43）, 79.07%（34/43） and 81.40%（35/43）, respectively, which were significantly higher than those of control group. The positive PARG was correlated to PARP（r=0.3703, P〈0.05） and b-FGF （r=0.4838, P〈0.05）. The positive PARP was correlated to VEGF （r=0.3968, P〈0.05） and b-FGF （r=0.5610, P〈0.05）. Both PARG and PARP were expressed in CT26 cells. The positive staining rates of PARG and PARP in GLTN-treated group were 7.3% and 52.38%, respectively. They were markedly reduced than those of control group （55.41% and 95.28%, P〈0.01, n=10000）. Conclusion： The data suggest that PARG expression probably plays a role for VEGF and b-FGF expression in colorectal carcinoma.

关键词： PARG PARP VEGF b-FGF Colorectal carcinoma

Review:Mitochondrial functions on oocytes and preimplantation embryos

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第7期10卷 483-492页

作者： li-ya wang Da-hui wang Xiang-yang ZOU Chen-ming XU Department of Biotechnology Dalian Medical University Dalian 116044 China The Sperm Laboratory Institute of Cell Biology and Genetics College of Life Sciences Zhejiang University Hangzhou 310058 China Department of Reproductive Endocrinology Women's Hospital School of Medicine Zhejiang University Hangzhou 310006 China

Oocyte quality has long been considered as a main limiting factor for in vitro fertilization （IVF）. In the past decade, extensive observations demonstrated that the mitochondrion plays a vital role in the oocyte cytoplasm, for it can provide adenosine triphosphate （ATP） for fertilization and preimplantation embryo development and also act as stores of intracellular calcium and proapoptotic factors. During the oocyte maturation, mitochondria are characterized by distinct changes of their distribution pattern from being homogeneous to heterogeneous, which is correlated with the cumulus apoptosis. Oocyte quality decreases with the increasing maternal age. Recent studies have shown that low quality oocytes have some age-related dysfunctions, which include the decrease in mitochondrial membrane potential, increase of mitochondrial DNA （mtDNA） damages, chromosomal aneuploidies, the incidence of apoptosis, and changes in mitochoncLrial gene expression. All these dysfunctions may cause a high level of de- velopmental retardation and arrest of preimplantation embryos. It has been suggested that these mitochondrial changes may arise from excessive reactive oxygen species （ROS） that is closely associated with the oxidative energy production or calcium overload, which may trigger permeability transition pore opening and subsequent apoptosis. Therefore, mitochondria can be seen as signs for oocyte quality evaluation, and it is possible that the oocyte quality can be improved by enhancing the physical function of mitochondria. Here we reviewed recent advances in mitochondrial functions on oocytes.

关键词： Mitochondria Oocyte Preimplantation embryo

Protein-protein Interaction Between Domains of PDZ and BAR from PICK1

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Chemical Research in Chinese Universities 2007年第2期23卷 191-195页

作者： XIAO Hong SHI ya-wei wang li-li YUAN Jing-ming Institute of Biotechnology Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education Shanxi University Taiyuan 030006 P. R. China

Two DNA fragments encoding PDZ domain （21-110 residues） and BAR domain （ 150-360 residues） from PICK1 （1-416 residues） were amplified by PCR and then introduced into vectors, pET-32M and pMAL-e2X respectively to generate recombinant plasmids, pE-pdz and pM-bar. Having been separately transferred into the hosts E. coli BL21 and E. coli JM109, these two strains can express fusion proteins： His-tagged PDZ（PDZ domain） and maltose binding protein-BAR（ MBP-BAR domain） respectively, as confirmed by both SDS-PAGE and Wostem blotting. The interaction between these two domains is dose-dependence, as identified by a pull-down test. Moreover, it has been shown from the EliSA analysis that the actual amount of PDZ bound to MBP-BAR-amylose beads reaches （ 16 ± 0. 5）%, as calculated by the molar ratio of PDZ to MBP-BAR. In addition, the interaction between BAR（bait） and PDZ（prey） in vivo was also examined with a yeast two-hybrid system.

关键词： BAR domain PDZ domain PICK1 Protein-protein interaction Pull-down test Yeast two-hybrid

Research on Mechanisms of Chinese Medicines in Prevention and Treatment of Postoperative Adhesion

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Chinese Journal of Integrative Medicine 2023年第6期29卷 556-565页

作者： ya-li wang wang ya-li ZHANG Hui-xiang CHEN yan-qi yaNG li-li li Zheng-jun ZHAO Min li Wen-lin BIAN yao-yao ZENG li School of First Clinical Medicine Nanjing University of Chinese MedicineNanjing(210023)China Jingwen library Nanjing University of Chinese MedicineNanjing(210023)China College of Health Economics Management Nanjing University of Chinese MedicineNanjing(210023)China School of Chinese Medicine School of Integrated Chinese and Western MedicineNanjing University of Chinese MedicineNanjing(210023)China College of Acupuncture and Massage College of Regimen and RehabilitationNanjing University of Chinese MedicineNanjing(210023)China Faculty of Chinese Medicine Macao University of Science and TechnologyTaipaMacao(999078)China Jiangsu Provincial Engineering Center of Traditional Chinese Medicine External Medication Researching and Industrializing Nanjing University of Chinese MedicineNanjing(210023)China

Postoperative adhesion(PA)is currently one of the most unpleasant complications following surgical *** have developed several new strategies to alleviate the formation of PA to a great extent,but so far,no single measure or treatment can meet the expectations and requirements of clinical patients needing complete PA *** medicine(CM)has been widely used for thousands of years based on its remarkable efficacy and indispensable advantages CM treatments are gradually being accepted by modern ***,this review summarizes the formating process of PA and the efficacy and action mechanism of CM treatments,including their pharmacological effects,therapeutic mechanisms and advantages in PA *** aim to improve the understanding of clinicians and researchers on CM prevention in the development of PA and promote the in-depth development and industrialization process of related drugs.

关键词： postoperative adhesion Chinese medicine prevention treatment review

Thoracoscopic segmentectomy assisted by three-dimensional computed tomography bronchography and angiography for lung cancer in a patient living with situs inversus totalis: A case report

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World Journal of Clinical Cases 2019年第22期7卷 3844-3850页

作者： Yun-Jiang Wu yang Bao ya-li wang Department of Thoracic Surgery The Affiliated Hospital of Yangzhou UniversityYangzhou UniversityYangzhou 225009Jiangsu ProvinceChina Department of Respiratory Medicine The Affiliated Hospital of Yangzhou UniversityYangzhou UniversityYangzhou 225009Jiangsu ProvinceChina

BACKGROUND Situs inversus totalis(SIT)is a rare congenital condition that is characterized by a complete mirror image of the typical arrangement of the thoracic and abdominal *** thoracoscopic segmentectomy for a patient with lung cancer and SIT is an extremely skilled and challenging surgical *** SUMMARY A 41-year old woman with a medical history of dextrocardia since childhood was admitted to our hospital with a mixed ground-glass opacity(mGGO)in her left lung field,discovered by computed tomography during her health *** order to facilitate surgical orientation,three-dimensional computed tomography bronchography and angiography(3D-CTBA)was preoperatively carried *** result of 3D-CTBA was consistent with the diagnosis of SIT and an mGGO in the posterior segment of the left upper lobe(LS2).Surgery was conducted in accordance with preoperative 3D-CTBA and designed surgical procedure,combined with intraoperative *** pathological examination revealed in situ *** patient’s postoperative condition was uneventful and no complications were *** We present the first case of lung cancer in a patient with SIT who successfully underwent thoracoscopic segmentectomy assisted by *** is a new technique that covers precise confirmation and dissection of targeted structures and intersegmental demarcation,and can help achieve a meticulous anatomical segmentectomy.

关键词： Situs inversus totalis Three-dimensional computed tomographybronchography and angiography Thoracoscopic segmentectomy Lung cancer Casereport

Using bovine pituitary extract to increase proliferation of keratocytes and maintain their phenotype in vitro

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International Journal of Ophthalmology(English edition) 2013年第6期6卷 758-765页

作者： Zhong-Zhong Xu Zhi-Jie li lian-Xin Du Jing li li-ya wang Department of Ophthalmology the First Affiliated Hospital of Zhengzhou University Key Laboratory of Corneal Disease Henan Eye Institute Department of Pediatrics Baylor College of Medicine

AIM:To investigate the effects of bovine pituitary extract on the proliferation of keratocytes and maintaining the keratocyte phenotype/n v/***:Single keratocytes were isolated by enzyme digestion for in vitro *** groups were designed according to the different culture media:a bovine pituitary extract(BPE)group,a fetal bovine serum(FBS)group and the control *** phenotypes and proliferation of cultured cells were evaluated by morphology,immunofluorescent staining and mRNA expression of CD34,Lumican,VSX1,α-SMA and proliferating cell nuclear antigen(PCNA).in the BPE group,cells underwent serial subcultivation,and their phenotypes were identified by immunofluorescent *** analyze the proliferation of keratocytes in different concentrations of BPE,six different concentrations were designed to ascertain the most appropriate ***:In the BPE group,the cells spread out and presented dendritic morphology,and their dendrites connected to one another to form *** the third passage,most cells maintained their *** the FBS group,the cells exhibited a dendritic appearance in early cultured stages,but their morphology subsequently changed into a fibroblast-like *** number ofdendritic cells in BPE group was more than FBS and control *** staining and realtime polymerase chain reaction(PCR)confirmed that few keratocytes underwent fibroblastic transformation in the BPE and control groups,and that proliferation was higher in the BPE group than in the control *** the proliferation was higher in the FBS group,many keratocytes underwent fibroblastic *** analysis of cell morphology and mRNA expressions of CD34,PCNA and VSX1 in six group showed that different concentrations of BPE affected the proliferation obviously but didn’t affect the keratocyte phenotype,and the concentration of 40μg/mL was the most appropriate ***:BPE can improve the proliferation of keratocytes and m

关键词： keratocyte bovine pituitary extract proliferation phenotype fibroblastic transformation

Laparoscopic vs open surgery for gastric cancer: Assessing time, recovery, complications, and markers

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World Journal of Gastrointestinal Surgery 2024年第1期16卷 40-48页

作者： Yun-yao Lu Yun-Xiao li Meng He ya-li wang Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment Chongqing University Cancer HospitalChongqing 400030China

BACKGROUND Gastric cancer(GC)is one of the most common cancers *** and mortality have increased in recent years,making it an urgent issue to ***-paroscopic radical surgery(LRS)is a crucial method for treating patients with GC;However,its influence on tumor markers is still under *** data of 194 patients treated at Chongqing University Cancer Hospital bet-ween January 2018 and January 2019 were retrospectively *** who underwent traditional open surgery and LRS were assigned to the control(n=90)and observation groups(n=104),*** sample t-tests andχ2 tests were used to compare the two groups based on clinical efficacy,changes in tumor marker levels after treatment,clinical data,and the incidence of posto-perative *** investigate the association between tumor marker levels and clinical efficacy in patients with GC,three-year recurrence rates in the two groups were *** Patients in the observation group had a shorter duration of operation,less in-traoperative blood loss,an earlier postoperative eating time,and a shorter hospital stay than those in the control group(P0.05).After treatment,the overall response rate in the control group was significantly lower than that in the observation group(P=0.001).Furthermore,after treatment,the levels of carbohydrate antigen 19-9,cancer antigen 72-4,carcinoembryonic antigen,and cancer antigen 125 decreased *** observation group also exhibited a significantly lower incidence rate of postoperative complications compared to the control group(P0.05).CONCLUSION LRS effectively treats early gastric cancer by reducing intraoperative bleeding,length of hospital stays,and postoperative complications.I

关键词： Laparoscopic radical surgery Gastric cancer Serum tumor markers Prognosis Recurrence Intraoperative bleeding

Thoracoscopic segmentectomy and lobectomy assisted by threedimensional computed-tomography bronchography and angiography for the treatment of primary lung cancer

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World Journal of Clinical Cases 2021年第34期9卷 10494-10506页

作者： Yun-Jiang Wu Qing-Tong Shi Yong Zhang ya-li wang Department of Thoracic Surgery The Affiliated Hospital of Yangzhou UniversityYangzhou UniversityYangzhou 225009Jiangsu ProvinceChina Department of Radiology The Affiliated Hospital of Yangzhou UniversityYangzhou UniversityYangzhou 225009Jiangsu ProvinceChina Department of Respiratory Medicine Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China

BACKGROUND Anatomical segmentectomy has been proposed as a substitution for lobectomy for early-stage lung ***,it requires technical meticulousness due to the complex anatomical variations of segmental vessels and *** To assess the safety and feasibility of three-dimensional computed-tomography bronchography and angiography(3D-CTBA)in performing video-assisted thoracoscopic surgery(VATS)for lung *** In this study,we enrolled 123 patients who consented to undergo thoracoscopic segmentectomy and lobectomy assisted by 3D-CTBA between May 2017 and June *** image data of enhanced computed tomography(CT)scans was reconstructed three-dimensionally by the Mimics *** results of preoperative 3D-CTBA,in combination with intraoperative navigation,guided the *** A total of 59 women and 64 men were enrolled,of whom 57(46.3%)underwent segmentectomy and 66(53.7%)underwent *** majority of tumor appearance on CT was part-solid ground-glass nodule(pGGN;55.3%).The mean duration of chest tube placement was 3.5±1.6 d,and the average length of postoperative hospital stay was 6.8±1.8 *** complications included one case of pneumonia and four cases of prolonged air leak lasting>5 ***,there was no intraoperative massive hemorrhage,postoperative intensive-care unit stay,or 30-d *** 3D-CTBA images can display clearly and vividly the targeted structure and the variations of vessels and *** reduce the risk of locoregional recurrence,the application of 3D-CTBA with a virtual 3D surgical margin help the VATS surgeon determine accurate distances and positional relations among the tumor,bronchial trees,and the intersegmental ***-dimensional navigation was performed to confirm the segmental structure,precisely cut off the targeted segment,and avoid intersegmental veins *** VATS and 3D-CTBA worked in harmony in our *** combination also provided a new pattern of transi

关键词： Thoracoscopy Segmentectomy Lobectomy Three-dimensional computed tomography Bronchography and angiography