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Temporal changes in soil bacterial and archaeal communities with different fertilizers in tea orchards

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2014年第11期15卷 953-965页

作者： Hua wang Shao-hui yaNG Jing-ping yaNG ya-min LV Xing ZHAO Ji-liang PANG Institute of Environmental Protection College of Environmental and Resource Sciences Zhejiang University College of Life and Environmental Sciences Hangzhou Normal University

It is important to understand the effects of temporal changes in microbial communities in the acidic soils of tea orchards with different fertilizers. A field experiment involving organic fertilizer （OF）, chemical fertilizer （CF）, and unfertilized control （CK） treatments was arranged to analyze the temporal changes in the bacterial and archaeal communities at bimonthly intervals based on the 16S ribosomal RNA （rRNA） gene using terminal restriction fragment length polymorphism （T-RFLP） profiling. The abundances of total bacteria, total archaea, and selected functional genes （bacterial and archaeal amoA, bacterial narG, nirK, nirS, and nosZ） were determined by quantitative poly- merase chain reaction （qPCR）. The results indicate that the structures of bacterial and archaeal communities varied significantly with time and fertilization based on changes in the relative abundance of dominant T-RFs. The abundancy of the detected genes changed with time. The total bacteria, total archaea, and archaeal amoA were less abundant in July. The bacterial amoA and denitrifying genes were less abundant in September, except the nirK gene. The OF treatment increased the abundance of the observed genes, while the CF treatment had little influence on them. The soil temperature significantly affected the bacterial and archaeal community structures. The soil moisture was signif- icantly correlated with the abundance of denitrifying genes. Of the soil chemical properties, soil organic carbon was the most important factor and was significantly correlated with the abundance of the detected genes, except the nirK gene. Overall, this study demonstrated the effects of both temporal alteration and organic fertilizer on the structures of mi- crobial communities and the abundance of genes involved in the nitrogen cycle.

关键词： Bacterial and archaeal communities Fertilizer Soil Temporal changes Tea orchard Functional genes

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Role of tissue factor in hepatocellular carcinoma genesis, invasion and metastasis

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Chinese Medical Journal 2011年第22期124卷 3746-3751页

作者： ZHOU Qi HUANG Tao wang ya-feng ZHOU Xiang-bing LIANG Li-jian PENG Bao-gang Department of Hepatobiliary Surgery First Affiliated Hospital of Sun Yat-Sen University Guangzhou Guangdong 510080 China

Background Numerous studies indicate that tissue factor （TF）, namely tissue thromboplastin, has a close relationship with malignant tumor genesis and progress. It contributes to blood coagulation as well as the regulation of cellular differentiation, the formation of blood vessels, and also tumor recurrence and metastasis. The present study aimed to detect TF expression in hepatocellular carcinoma （HCC） patients and to elucidate its association with prognosis and clinical features of the disease. Methods The plasma TF levels of 50 HCC patients and 30 controls were assayed by ELISA. The expressions of TF mRNA and protein in HCC tissues, adjacent tissues and normal tissues were detected by reverse transcription- polymerase chain reaction （RT-PCR） and Western blotting. The acquired data were analyzed with related clinic-pathological documents. The patients were followed up for five years, and the relationship between TF and prognosis was analyzed. Results The plasma TF levels were significantly increased in HCC compared to the controls （P 〈0.05）, presenting a close relationship with differentiation level, tumor size and hepatocirrhosis occurrence （P 〈0.05）. There were remarkably higher values in cases of lymphatic metastasis, extrahepatic metastasis and portal tumor thrombus （PTT）（P 〈0.05） compared to non-metastasis or non-tumor thrombus, but no significant difference with different focus number or envelope （P 〉0.05）. The positive rates and the relative expression of TF mRNA in HCC tissue were 63.0% （17/27） and 0.567±0.268, respectively, significantly higher than that in adjacent tissues or normal tissues （P 〈0.05）. In the patients with positive results, the relative expression intensity varied significantly with different tumor size and index of local invasion and metastasis （P 〈0.05）. The positive rates and the relative expression intensities of TF protein in HCC tissue were 74.1% （20/27） and 4.093±1.256, respectively, significantly higher than those in adjacent tis

关键词： hepatocellular carcinoma tissue factor invasion and metastasis

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Finite-time coordinated path-following control of leader-following multi-agent systems

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Frontiers of Information Technology & Electronic Engineering 2022年第10期23卷 1511-1521页

作者： Weibin CHEN yangyang CHEN ya ZHANG School of Automation Southeast UniversityNanjing 210096China Key Laboratory of Measurement and Control of Complex Systems of Engineering Ministry of EducationSoutheast UniversityNanjing 210096China Robot Department Jiangsu Automation Research InstituteLianyungang 222061China

This paper presents applications of the continuous feedback method to achieve path-following and a formation moving along the desired orbits within a finite *** is assumed that the topology for the virtual leader and followers is *** additional condition of the so-called barrier function is designed to make all agents move within a limited area.A novel continuous finite-time path-following control law is first designed based on the barrier function and *** a novel continuous finite-time formation algorithm is designed by regarding the path-following errors as *** settling-time properties of the resulting system are studied in detail and simulations are presented to validate the proposed strategies.

关键词： Finite-time Coordinated path-following Multi-agent systems Barrier function

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Comparison of the anti-inflammatory effects of fluorometholone 0.1% combined with levofloxacin 0.5% and tobramycin/dexamethasone eye drops after cataract surgery

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International Journal of Ophthalmology(English edition) 2016年第11期9卷 1619-1623页

作者： Pei-Qing Chen Xue-Mei Han ya-Nan Zhu Jia Xu Eye Center the Second Affiliated Hospital of Zhejiang University School of Medicine Hangzhou 310009 Zhejiang Province China Zhejiang Hospital of Traditional Chinese Medicine Hangzhou 310006 Zhejiang Province China

AIM： To compare the combination of fluorometholone0.1% and levofloxacin 0.5% to tobramycin/dexamethasone eye drops in controlling inflammation and preventing infection after phacoemulsification with an intraocular lens ***： Sixty eyes from 60 patients undergoing cataract phacoemulsification were randomized into two groups; half of the patients were treated with fluorometholone（6 times/d） combined with levofloxacin（4 times/d）, while the other half were treated with tobramycin/dexamethasone（4 times/d） eye drops for one week. Preoperative and postoperative intraocular pressure, aqueous flare, corneal thickness, and signs and symptoms were recorded before the operation and1 wk following treatments. RESULTS： There were no statistically significant differences between the two groups in corneal thickness（P ≥0.629）, aqueous flare（P ≥0.398）, and signs and symptoms scores（P ≥0.350） at each time point. Ocular hypertension was only observed in two eyes in the tobramycin/dexamethasone group. CONCLUSION： Fluorometholone combined with levofloxacin treatment shows comparable efficacy but without the tendency to increase intraocular pressure;thus, it might be a better regimen for postoperative use.

关键词： fluorometholone levofloxacin tobramycin/dexamethasone phacoemulsification inflammation

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强磁场对Al-Si合金凝固组织中硅分布的影响

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物理学报 2006年第2期55卷 648-654页

作者：王春江王强王亚勤黄剑赫冀成东北大学材料电磁过程研究教育部重点实验室沈阳110004 沈阳铸造研究所沈阳110021

为了揭示强磁场对金属凝固组织的影响规律,本文研究了Al-14.98%Si(质量分数)和Al-9.2%Si(质量分数)合金在强磁场作用下凝固组织的变化趋势,分析了强磁场对合金凝固组织中Si分布的影响.研究发现,均恒磁场和梯度磁场分别通过洛伦兹力和磁... 详细信息

为了揭示强磁场对金属凝固组织的影响规律,本文研究了Al-14.98%Si(质量分数)和Al-9.2%Si(质量分数)合金在强磁场作用下凝固组织的变化趋势,分析了强磁场对合金凝固组织中Si分布的影响.研究发现,均恒磁场和梯度磁场分别通过洛伦兹力和磁化力的作用对合金的凝固组织产生影响,强磁场可以显著改变初晶硅在合金中的分布状况.在均恒磁场作用条件下初晶硅在合金中均匀分布;在梯度磁场条件下,由于磁化力和浮力的共同作用,初晶硅在试样的上部或下部聚集.同时,磁化力也改变了共晶体在合金中的组织形态,使试样上部和下部共晶体的层片间距明显不同.理论和实验分析表明,Al-Si合金在强磁场中凝固时,磁场能作用于凝固过程,使共晶体中的Al含量增大,共晶点向左偏移.

关键词：强磁场凝固过程共晶组织 Al-Si合金

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Shen-Fu injection reduces impaired myocardial β-adrenergic receptor signaling after cardiopulmonary resuscitation

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Chinese Medical Journal 2013年第4期126卷 697-702页

作者： JI Xian-fei JI Hong-bin SANG De-ya wang Shuo yaNG Lin LI Chun-sheng Department of Emergency Medicine Provincial Hospital Affiliated to Shandong University Jinan Shandong 250021 China Department of Emergency Medicine Beijing Chaoyang Hospital Capital Medical University Beijing 100020 China

Background Post-resuscitation myocardial dysfunction has been implicated as a major cause of fatal outcome in patients who survive initially successful cardiopulmonary resuscitation （CPR）. In our previous study, we found that impaired myocardial β-adrenergic receptor （AR） signaling is a key mechanism in post-resuscitation myocardial dysfunction and Shen-Fu injection （SFI） can attenuate post-resuscitation myocardial dysfunction. However, whether SFI can prevent impaired post-resuscitation myocardial β-AR signaling is not yet known. In this study, we investigated the effect of SFI on impaired myocardial β-AR signaling occurring post-resuscitation in a porcine model of cardiac arrest. Methods Ventricular fibrillation was induced electrically in anesthetized male landrace domestic pigs. After 4 minutes of untreated ventricular fibrillation, cardiopulmonary resuscitation was initiated. Sixteen successfully resuscitated pigs were randomized to receive a continuous infusion of either SFI （0.5 ml/min; n=8） or saline （placebo; n=8） for 6 hours, beginning 15 minutes after the return of spontaneous circulation （ROSC）. Hemodynamic and echocardiographic data were recorded. β-AR signaling was assessed at 6 hours after the intervention by measuring myocardial adenylate cyclase activity, β-AR density and β-AR kinase expression. Results Treatment with SFI produced better maximum rate of left ventricular pressure increase （dp/dtmax） and maximum rate of left ventricular pressure decline （-dp/dtmax）, cardiac output, and ejection fraction after ROSC. SFI treatment was also associated with lower myocardial β-adrenergic receptor kinase expression, whereas basal and isoproterenol- stimulated adenylate cyclase activity and the total β-AR density were significantly increased in the SFI group when compared with the placebo group. Conclusion SFI attenuated post-resuscitation myocardial dysfunction by preventing impaired myocardial β-AR signaling after CPR.

关键词： cardiopulmonary resuscitation myocardial β-adrenergic receptor signaling Shen-Fu injection

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Automatic sweet pepper detection based on point cloud images using subtractive clustering

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International Journal of Agricultural and Biological Engineering 2020年第3期13卷 154-160页

作者： Xiaokang Zhao Hao Li Qibing Zhu Min Huang ya Guo Jianwei Qin Key Laboratory of Advanced Process Control for Light Industry of Ministry of Education Jiangnan UniversityWuxi214122China USDA/ARS Environmental Microbial and Food Safety Laboratory Beltsville Agricultural Research CenterBldg.303BARC-East10300 Baltimore Ave.MD 20705-2350USA

Automatic identification and detection of fruit on trees by machine vision is the basis of developing automatic harvesting robots in *** occlusion of branches,leaves and other fruits in canopy images will affect the accuracy of fruit *** provide a scientific and reliable technical guidance for fruit harvesting robots,a method using point cloud images was proposed in this study to detect red fruits to overcome the impact of occlusion on ***,the fruit regions were segmented from a tree’s point cloud by applying the color threshold of red and ***,the noise in fruit point clouds was removed with sparse outlier ***,the point cloud of each fruit was detected and counted based on the subtractive clustering *** the sweet pepper dataset,the true positive rate(TPR)is 90.69%and the false positive rate(FPR)is 6.97%for all fruits that are at least partially visible in the scene.

关键词： sweet pepper detection point cloud subtractive clustering computer vision

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Does persistence of showy calyces in Limonium leptolobum enhance pollinator attraction?

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Journal of Plant Ecology 2015年第2期8卷 182-186页

作者： Ai-Qin Zhang Shuang He ya-Xin Zhai Shuang-Quan Huang College of Life Science and Technology Xinjiang UniversityNo.14 Victory AvenueUrumqi 830046China School of Life Sciences Central China Normal University152 Luoyu AvenueHongshan DistrictWuhan 430079China

Aims The calyx,the outermost whorl of a flower(usually green),has been considered to function to protect *** some species,how-ever,calyces are colorful and retained during seed *** species have been exploited as cut flower crops because the calyces persist for several months after the corolla has *** explore the adaptive significance of the persistent calyx in a desert plant Limonium leptolobum,we ask whether persistence of caly-ces can enhance pollinator attraction by enlarging floral displays,increasing reproductive success in this self-incompatible *** The yellow flower of *** lasted 1-2 days but its white,membranous calyx extended fully after the corolla closed,and per-sisted for over 2 months in the field,making hundreds of‘showy flowers’on one *** examine the ecological function of calyces,we test the pollinator attraction *** an experi-mental population,we compared the difference in visit frequency and visitor behavior between intact inflorescences and inflores-cences with their calyces removed on the same individual *** findingsin four experimental plots four types of floral visitors were observed including bees,butterflies,syrphid flies and day-flying *** significant preference was observed between calyx-free and intact inflorescences for both first arrivals and total visit frequency of all types of floral visitors,indicating that the persistence of calyces did not make plants more attractive to potential *** pollina-tor attraction hypothesis for the showy calyces was not supported by the current *** the calyx in this desert plant helps seed development or has other functions needs further study.

关键词： Limonium leptolobum calyx floral traits pollinator preference advertisement ecological function

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Ethambutol-induced optic neuropathy with rare bilateral asymmetry onset:A case report

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World Journal of Clinical Cases 2022年第2期10卷 663-670页

作者： Wen-yan Sheng Shuang-Qing Wu Ling-ya Su Li-Wei Zhu Department of Ophthalmology Affiliated Hangzhou Chest HospitalZhejiang University School of MedicineHangzhou 310003Zhejiang ProvinceChina

BACKGROUND Ethambutol-induced optic neuropathy(EON)most commonly manifests as bilateral symmetrical loss of vision and often cause serious and irreversible visual impairment because of the lack of early detection and effective *** followed a case of EON with rare binocular asymmetric clinical manifestations and observed the changes of visual function and retinal structure after drug withdrawal,so as to further understand the clinical characteristics of this *** SUMMARY A 54-year-old man complained of gradual visual decline in the left *** patient presented with best-corrected visual acuity of 20/20 in the right eye and 20/50 in the left *** vision examination revealed difficulty in reading green color plates in the left *** visual field manifested as concentric contraction in the left *** nearly a month of drug withdrawal,the right eye had a similar decline in visual *** the last visit,19 mo after drug withdrawal,the visual function significantly recovered in both *** follow-up optical coherence tomography(OCT)examination,both eyes manifested the thickness of the retinal nerve fiber layer from mild thickening to thinning and finally temporal atrophy,and the ganglion cell-inner plexiform layer showed significant *** difference was that a reversible structural disorder in the outer retina of the nasal macula was detected in the left eye by macular high-definition *** Nephropathy and high blood pressure,which damage the retinal microcirculation,may cause damage to the outer layer of the *** may influence photoreceptor as well as retinal ganglion cells.

关键词： Ethambutol-induced optic neuropathy Retinal nerve fiber layer Ganglion cell-inner plexiform layer Optical coherence tomography Asymmetry Case report

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Preparation, Properties and Mechanism of Inhomogeneous Calcium Alginate Ion Cross-linking Gel Microspheres

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Chemical Research in Chinese Universities 2003年第1期19卷 85-88页

作者： CHEN Yi qing SUN Duo xian LIU Jun HUANG ya qing School of Chemical Engineering and Technology Tianjin UniversityTianjin 300072P.R.China

Inhomogeneous calcium alginate ion cross\|linking gel microspheres, a novel ion absorbent, were prepared by dropping a sodium alginate solution to a calcium chloride solution via an electronic droplet generator. Calcium alginate microspheres have uniform particle sizes, a smooth surface and a microporous structure. The electrode probe reveals the inhomogeneous distribution of calcium ions with the highest concentration on the surface, and the lowest concentration in the cores of the spheres. As a novel ion adsorbent, calcium alginate gel microspheres have a lower limiting adsorption mass concentration, a higher enrichment capacity and a higher adsorption capacity for Pb 2+ than usual ion exchange resins. The highest percentage of the adsorption is 99 79%. The limiting adsorption mass concentration is 0 0426 mg/L. The adsorption capacity for Pb 2+ is 644 mg/g. Calcium alginate gel microspheres have a much faster ion exchange velocity than D418 chelating resin and D113 polyacrylate resin. The moving boundary model was employed to interpret the ion exchange kinetics process, which indicates that the ion exchange process is controlled by intraparticle diffusion of adsorbable ions. So the formation of inhomogeneous gel microspheres reduces the diffusion distance of adsorbable ions within the spheres and enhances the ion exchange velocity. Alginate has a higher selectivity for Pb 2+ than for Ca 2+ and the selectivity coefficient K Pb Ca is 316. As an ion cross\|linking gel, calcium alginate inhomogeneous microspheres can effectively adsorb heavy metal Pb 2+ at a higher selectivity and a higher adsorption velocity. It is a novel and good ion adsorbent.

关键词： Alginate Inhomogeneous microsphere Adsorption Mechanism

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