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Participation of the inositol 1,4,5-trisphosphategated calcium channel in the zona pellucida- and progesterone-induced acrosome reaction and calcium influx in human spermatozoa

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Asian Journal of Andrology 2020年第2期22卷 192-199页

作者： ying-ya li yan-Ping Jia li-yan Duan Kun-Ming li Center of Reproductive Medicine Shanghai First Maternity and Infant HospitalTongji University School of MedicineShanghai 201204China

The acrosome reaction is a prerequisite for fertilization,and its signaling pathway has been investigated for *** of the type of inducers present,the acrosome reaction is ultimately mediated by the elevation of cytosolic *** 1,4,5-trisphosphate-gated calcium channels are important components of the acrosome reaction signaling pathway and have been confirmed by several *** this study,we used a novel permeabilization tool BioPORTER?and first demonstrated its effectiveness in *** inositol 1,4,5-trisphosphate type-1 receptor antibody was introduced into spermatozoa by BioPORTER?and significantly reduced the calcium influx and acrosome reaction induced by progesterone,solubilized zona pellucida,and the calcium ionophore *** finding indicates that the inositol 1,4,5-trisphosphate type-1 receptor antibody is a valid inositol 1,4,5-trisphosphate receptor inhibitor and provides evidence of inositol 1,4,5-trisphosphate-gated calcium channel involvement in the acrosome reaction in human ***,we demonstrated that the transfer of 1,4,5-trisphosphate into spermatozoa induced acrosome reactions,which provides more reliable evidence for this *** addition,by treating the spermatozoa with inositol 1,4,5-trisphosphate/BioPORTER?in the presence or absence of calcium in the culture medium,we showed that the opening of inositol 1,4,5-trisphosphate-gated calcium channels led to extracellular calcium *** particular extracellular calcium influx may be the major process of the final step of the acrosome reaction signaling pathway.

关键词： acrosome reaction human spermatozoa inositol 1,4,5-trisphosphate zona pellucida progesterone

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Short-term efficacy and influencing factors of conventional chemotherapy combined with irinotecan in patients with advanced gastric cancer

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World Journal of Gastrointestinal Oncology 2023年第1期15卷 143-154页

作者： Jun-Ping Wang Jian-Lei Du ya-ying li Pharmacy Department Zhumadian Central HospitalZhumadian 463000Henan ProvinceChina

BACKGROUND Gastric cancer is one of the most common cancers worldwide, with a 5-year survival rate of only 20%. The age of onset of gastric cancer is in line with the general rule of cancer. Most of them occur after middle age, mostly between 40and 60 years old, with an average age of about 50 years old, and only 5% of patients are under 30 years old. The incidence of male is higher than that of *** To investigate the short-term efficacy and influencing factors of chemotherapy combined with irinotecan in patients with advanced gastric *** Eighty patients with advanced gastric cancer who were treated in our hospital from January 2019 to January 2022 were selected. The patients were divided into an observation group(n = 40) and control group(n = 40) by the envelope *** control group was given preoperative routine chemotherapy. The observation group was treated with irinotecan in addition to the chemotherapy given to the control group. The short-term efficacy of treatment in the two groups, as well as tumor marker levels and quality of life before and after treatment were *** The short-term treatment effect in the observation group was better than that in the control group(P < 0.05), and the total effective rate was 57.50%. The age and proportion of tumor node metastasis(TNM) stage IV patients with ineffective chemotherapy in the observation group were(65.12 ± 5.71) years and 52.94%,respectively, which were notably higher than those of patients with effective chemotherapy(P < 0.05), while the Karnofsky Performance Scale score was(67.70± 3.83) points, which was apparently lower than that of patients with effective chemotherapy(P <0.05). After 3 mo of treatment, the SF-36 scale scores of physiological function, energy, emotional function, and mental health in the observation group were 65.12 ± 8.14, 54.76 ± 6.70, 47.58 ± 7.22,and 66.16 ± 8.11 points, respectively, which were considerably higher than those in the control group(P < 0.

关键词： Advanced gastric cancer Conventional chemotherapy Irinotecan Efficacy Quality of life

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Protecting Cell Walls from Binding Aluminum by Organic Acids Contributes to Aluminum Resistance

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Journal of Integrative Plant Biology 2009年第6期51卷 574-580页

作者： ya-ying li Yue-Jiao Zhang Yuan Zhou Jian-li yang Shao-Jian Zheng Ministry of Agriculture Key Laboratory for Non-Point Pollution Control Zhejiang University Hangzhou 310029 China State Key Laboratory of Plant Physiology and Biochemistry College of Life Sciences Zhejiang University Hangzhou 310058 China

Aluminum-induced secretion of organic acids from the root apex has been demonstrated to be one major AI resistance mechanism in plants. However, whether the organic acid concentration is high enough to detoxify AI in the growth medium is frequently questioned. The genotypes of AI-resistant wheat, Cassia tora L. and buckwheat secrete malate, citrate and oxalate, respectively. In the present study we found that at a 35% inhibition of root elongation, the AI activities in the solution were 10, 20, and 50 μM with the corresponding malate, citrate, and oxalate exudation at the rates of 15, 20 and 21 nmol/cm2 per 12 h, respectively, for the above three plant species. When exogenous organic acids were added to ameliorate AI toxicity, twofold and eightfold higher oxalate and malate concentrations were required to produce the equal effect by citrate. After the root apical cell walls were isolated and preincubated in 1 mM malate, oxalate or citrate solution overnight, the total amount of AI adsorbed to the cell walls all decreased significantly to a similar level, implying that these organic acids own an equal ability to protect the cell walls from binding AI. These findings suggest that protection of cell walls from binding AI by organic acids may contribute significantly to AI resistance.

关键词： adsorption and desorption aluminum toxicity cell wall chelation detoxification organic acid.

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Advanced primary amelanotic malignant melanoma of the esophagus: A case report

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World Journal of Clinical Cases 2019年第19期7卷 3160-3167页

作者： Ruo-Xi Zhang ya-ying li Chang-Jie liu Wei-Na Wang ying Cao Yong-Hua Bai Ti-Jiang Zhang Department of Radiology Affiliated Hospital of Zunyi Medical UniversityZunyi 563003Guizhou ProvinceChina Department of Radiology Guizhou Provincial People's HospitalGuiyang 550002Guizhou ProvinceChina Department of Radiology West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Department of Pathology Guizhou Provincial People's HospitalGuiyang 550002Guizhou ProvinceChina Department of Pathology Affiliated Hospital of Zunyi Medical UniversityZunyi 563003Guizhou ProvinceChina

BACKGROUND Primary malignant melanoma of the esophagus accounts for 0.1%-0.2%of all esophageal malignancies,including melanotic and amelanotic *** amelanotic malignant melanoma of the esophagus is extremely rare,and only about 20 cases have been published in the literature to *** primary malignant melanomas of the esophagus are diagnosed following development of metastatic lesions and thus have a very poor *** median survival duration of patients with metastatic melanoma has been reported to be 6.2 *** SUMMARY A 49-year-old woman was referred to our hospital with a diagnosis of esophageal ***,biopsy,imaging evaluation,and physical examination at our hospital indicated a diagnosis of advanced primary amelanotic malignant melanoma of the *** staining confirmed *** medicine examination revealed a left iliac bone metastatic *** discharge,the patient self-administered apatinib for 3 mo,followed by oral treatment with Chinese medicines(also self-administered)for 2 *** treatments had been taken since *** patient has survived with no growth out to the most recent follow-up(24 mo post diagnosis),and she always presented with a positive attitude about her condition during this *** Survival following metastatic melanoma might be related to the pharmaceutical and Chinese medicine treatment and the patient's positive attitude.

关键词： Esophagus Amelanotic malignant melanoma Immune Emotion Psychological intervention Case report

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Inhibition of HBV targeted ribonuclease enhanced by introduction of linker

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World Journal of Gastroenterology 2003年第7期9卷 1504-1507页

作者： Wei-Dong Gong Jun liu Jin Ding ya Zhao ying-Hui li Cai-Fang Xue Department of Pathogenic Organisms,Fourth Military Medical University,Xi’an 710033,Shaanxi Province,China Department of Pathogenic Organisms Fourth Military Medical University Xi'an 710033 Shaanxi Province China.

AIM: To construct human eosinophil-derived neurotoxin (hEDN) and HBV core protein (HBVc) eukaryotic fusion expression vector with a linker (Gly4 Ser)3 between them to optimize the molecule folding, which will be used to inhibit HBV replication in ***: Previously constructed pcDNA3. 1(-)/TR was used as a template. linker sequence was synthesized and annealed to form dslinker, and cloned into pcDNA3.1(-)/TR to produce plasmid pcDNA3.1(-)/HBc-linker. Then the hEDN fragment was PCR amplified and inserted into pcDNA3.1(-)/HBc-linker to form pcDNA3.1(-)/TNL in which the effector molecule and the target molecule were separated by a linker sequence. pcDNA3.1(-)/TNL expression was identified by indirect immunofluorescence staining. Radioimmunoassay was used to analyse anti-HBV activity of pcDNA3.1(-)/***, metabolism of cells was evaluated by NTT ***: hEDN and HBVc eukaryotic fusion expression vector with a linker (Gly4Ser)3 between them was successfully constructed. pcDNA3.1(-)/TNL was expressed in HepG2.2.15 cells efficiently. A significant decrease of HBsAg concentration from pcDNA3.1(-)/TNL transfectant was observed compared to pcDNA3. 1(-)/TR (P=0.036, P＜0.05).MTT assay suggested that there were no significant differences between groups (P=0.08, P＞0.05).CONCLUSION: linker introduction enhances the inhibitory effect of HBV targeted ribonuclease significantly.

关键词：乙型肝炎病毒核糖核酸酶噬酸性细胞神经毒素

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波长与能量色散复合式X射线荧光光谱仪特性研究及矿区土壤分析

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光谱学与光谱分析 2017年第7期37卷 2216-2224页

作者：沈亚婷李迎春孙梦荷何玉君王艳飞蔺雅洁国家地质实验测试中心北京100037 中国地质大学(武汉)材料与化学学院湖北武汉430074

波长与能量色散(WD-ED)复合式X射线荧光(XRF)光谱仪是一型国际上新近研发的XRF光谱仪。该研究比较了此型光谱仪的特性,建立了联用分析方法,并用不确定度对所建方法进行了评估,证明WDEDXRF复合型光谱仪及其所建分析方法可用于土壤样品中... 详细信息

波长与能量色散(WD-ED)复合式X射线荧光(XRF)光谱仪是一型国际上新近研发的XRF光谱仪。该研究比较了此型光谱仪的特性,建立了联用分析方法,并用不确定度对所建方法进行了评估,证明WDEDXRF复合型光谱仪及其所建分析方法可用于土壤样品中主、次、痕量元素定量测定,并兼具了WD和ED各自的优点。研究表明:(1)对土壤质量和生态环境评价中具有重要意义的Mg,Al,P和K等元素,因WDXRF对轻元素具有更高灵敏度,故采用该型光谱仪和所建方法,可弥补单一采用ED方法的不足,从而为土壤质量和生态环境评价提供了更为灵敏和准确可靠的分析技术手段;(2)在本方法实验条件下,采用WDXRF测定,主元素Na2O,MgO,Al2O3,P2O5,K2O的检出限优于EDXRF,而SiO2,SO3,CaO,MnO,Fe2O3则是由EDXRF测定的检出限更优。对于谱线重叠较严重的微量元素,多数情况下WDXRF的检出限更低;(3)对主元素而言,总体上K及其原子序数之前的轻元素WDXRF准确度更好,Ca及其之后的元素EDXRF准确度更好。然而,由WDXRF和EDXRF获得的准确度也与样品相关,在某些情况下,EDXRF测定K的准确度会更好。微量元素和重叠干扰比较大的元素,采用分辨率好的WDXRF可获得更好的准确度;对于受其他元素谱线重叠影响较小的元素,EDXRF给出的结果准确度更好;(4)选用添加石蜡粘结剂的粉末压片法,利用该方法制成的地质样品粉末压片结实、无脱落,未观察到掉渣、掉粉现象,且分析准确度和精密度良好;(5)利用所建立的WD-EDXRF方法测定了采自集中开采矿区和附近河流及河漫滩沉积物,揭示矿区表层土含有较高浓度的Cu,Pb和Zn,可为找矿提供指示信息;(6)进行了矿区周边农田土壤分析,获得了元素分布趋势图,揭示矿集区附近农田土壤Pb和As等浓度较高,需要采取必要的生态与环境保护措施,以减小和避免对于人类健康的潜在影响。

关键词： X射线荧光土壤污染铅砷镉锌

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护理科研中实验动物应用现状分析

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实用护理杂志 2003年第18期9卷 1-2页

作者：廖晓艳李亚洁李瑛第一军医大学南方医院

目的探讨利用结肠黏膜一期尿道成形术治疗复杂性超长段尿道狭窄或闭锁的应用价值与疗效.方法 2000年10月～2003年5月采用结肠黏膜一期尿道成形术治疗19例复杂性超长段尿道狭窄患者.患者术前有平均3次不成功的尿道修复史,用结肠黏膜重建... 详细信息

目的探讨利用结肠黏膜一期尿道成形术治疗复杂性超长段尿道狭窄或闭锁的应用价值与疗效.方法 2000年10月～2003年5月采用结肠黏膜一期尿道成形术治疗19例复杂性超长段尿道狭窄患者.患者术前有平均3次不成功的尿道修复史,用结肠黏膜重建的尿道长10～17 cm,平均13.2 cm.术后随访时分别行逆行尿道造影,尿道镜和尿流率检查.结果本组患者术后随访6～36个月,平均17个月.1例在术后3个月并发尿道外口狭窄,经手术矫正后排尿通畅,术后1年随访时最大尿流率28.7 ml/s.另1例在术后14个月行尿道镜检查时发现精阜肥大,将肥大的精阜切除后行尿动力学检查示最大尿流率高达46.5 ml/s.余17例术后排尿通畅,最大尿流率大于15 ml/s.结论利用结肠黏膜重建尿道治疗复杂性超长段尿道狭窄或闭锁是一种可行而有效的方法,尤其适合在较多常规方法治疗失败时实施.

关键词：实验动物文献计量学护理科研实验设计

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甲苯二异氰酸酯的气相色谱-质谱分析

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分析测试学报 2001年第4期20卷 62-64页

作者：张伟亚李英刘丽深圳出入境检验检疫局工业品检测技术中心广东深圳518045

采用气相色谱 -质谱分析甲苯二异氰酸酯 (TDI) ,以TDI80为例 ,对测试过程中样品的存放、溶剂及色谱条件的选择、组分定量作了探讨 ,获得满意的结果。该法无需标样 ,具有定性定量准确。

关键词：气相色谱-质谱法 2,4-甲苯二异氰酸酯 2,6-甲苯二异氰酯酯分析

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Nanostructured ZnO/BiVO_(4)I-scheme heterojunctions for piezocatalytic degradation of organic dyes via harvesting ultrasonic vibration energy

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 488-497页

作者： Yiling li Xiaoyao Yu yingjie Zhou yao lin ying Wu Key Laboratory of the Ministry of Education for Advanced Catalysis Materials College of Chemistry and Materials ScienceZhejiang Normal UniversityJinhua 321004China

BiVO_(4)porous spheres modified by ZnO were designed and synthesized using a facile two-step *** resulting ZnO/BiVO_(4)composite catalysts have shown remarkable efficiency as piezoelectric catalysts for degrading Rhodamine B(RhB)unde mechanical vibrations,they exhibit superior activity compared to pure *** 40wt%ZnO/BiVO_(4)heterojunction composite displayed the highest activity,along with good stability and *** enhanced piezoelectric catalytic activity can be attributed to the form ation of an I-scheme heterojunction structure,which can effectively inhibit the electron-hole ***,hole(h+)and superoxide radical(·O_(2)^(-))are proved to be the primary active ***,ZnO/BiVO_(4)stands as an efficient and stable piezoelectric catalyst with broad potential application in the field of environmental water pollution treatment.

关键词： piezoelectric catalytic heterojunction dye degradation ultrasonic vibration

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Physical Layer Security Scheme With AoI-Awareness for Industrial IoT Based on Covert Communications

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IEEE/CAA Journal of Automatica Sinica 2025年第1期 276-278页

作者： yaping li Zhi-Xin liu Jia-Wei Su ya-Zhou Yuan the Key Laboratory of Industrial Computer Control Engineering of Hebei Province Yanshan University

Dear Editor,Industrial Internet of things(IIoT) is a typical application of cyberphysical system(CPS). In the IIoT, wireless communication is an inevitable trend to replace the deployment-limited wired transmission for cases with large-scale and mobile devices. However, wireless communication gives rise to critical issues related to physical security, such as malicious detections and attacks [1].

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