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Hepatitis B core antigen modulates exosomal miR-135a to target vesicle-associated membrane protein 2 promoting chemoresistance in hepatocellular carcinoma

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World Journal of Gastroenterology 2021年第48期27卷 8302-8322页

作者： xiao-Cui Wei ya-ru xia Ping Zhou Xing Xue Shuang Ding Li-Juan Liu Fan Zhu State Key Laboratory of Virology Hubei Province Key Laboratory of Allergy and ImmunologyDepartment of Medical MicrobiologySchool of MedicineWuhan UniversityWuhan 430071Hubei ProvinceChina

BACKGROUND Hepatocellular carcinoma(HCC)is one of the most common malignant *** association of hepatitis B virus(HBV)infection with HCC is hitherto *** miRNAs contribute to cancer progression and *** X protein has been known to modulate miRNAs that facilitate cell proliferation and the process of ***,there has been no report on hepatitis B core antigen(HBc)regulating exosomal miRNAs to induce drug resistance of HCC *** To elucidate the mechanism by which HBc promotes Doxorubicin hydrochloride(Dox)resistance in *** Exosomes were isolated by *** morphology and size of exosomes were evaluated by Dynamic Light Scattering(DLS)and transmission electron microscopy(TEM).The miRNAs differentially expressed in HCC were identified using The Cancer Genome Atlas(TCGA)*** level of miR-135a-5p in patient tissue samples was detected by quantitative polymerase chain *** and luciferase assay were used to predict and prove the target gene of ***,we identified the effects of miR-135a-5p on anti-apoptosis and the proliferation of HCC in the presence or absence of Dox using flow cytometry,Cell counting kit 8(CCK-8)assay and western *** We found that HBc increased the expression of exosomal *** analysis of bioinformatics and patient samples found that miR-135a-5p was increased in HCC tissues in comparison with paracancerous *** analysis and in vitro validation identified vesicle-associated membrane protein 2(VAMP2)as a novel target gene of *** assays showed that exosomal miR-135a-5p induced apoptosis protection,cell proliferation,and chemotherapy resistance in *** addition,the rescue experiment demonstrated that VAMP2 reversed apoptosis protection,cell growth,and drug resistance by ***,HBc promoted HCC anti-apoptosis,proliferation,and drug resistance and prevented Dox-i

关键词： Hepatocellular carcinoma Exosomes miR-135a-5p Anti-apoptosis Proliferation Chemoresistance

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CPEB1,a novel risk gene in recent-onset schizophrenia,contributes to mitochondrial complex I defect caused by a defective provirus ERVWE1

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World Journal of Psychiatry 2021年第11期11卷 1075-1094页

作者： ya-ru xia xiao-Cui Wei Wen-Shi Li Qiu-Jin yan Xiu-Lin Wu Wei yao Xu-Hang Li Fan Zhu State Key Laboratory of Virology and Hubei Province Key Laboratory of Allergy&Immunology Department of Medical MicrobiologySchool of MedicineWuhan UniversityWuhan 430071Hubei ProvinceChina

BACKGROUND Schizophrenia afflicts 1%of the world *** studies suggest that schizophrenia patients may have an imbalance of mitochondrial energy metabolism via inhibition of mitochondrial complex I ***,recent studies have shown that ERVWE1 is also a risk factor for ***,there is no available literature concerning the relationship between complex I deficits and ERVWE1 in *** risk factors and blood-based biomarkers for schizophrenia may provide new guidelines for early interventions and prevention *** To address novel potential risk factors and the underlying mechanisms of mitochondrial complex I deficiency caused by ERVWE1 in *** Quantitative polymerase chain reaction(qPCR)and enzyme-linked immunosorbent assay were used to detect differentially expressed risk factors in blood *** statistical analyses were performed by median analyses and Mann-Whitney U ***’s rank correlation was applied to examine the correlation between different risk factors in blood ***,western blot analysis,and luciferase assay were performed to confirm the relationship among ERVWE1,cytoplasmic polyadenylation element-binding protein 1(CPEB1),NADH dehydrogenase ubiquinone flavoprotein 2(NDUFV2),and NDUFV2 pseudogene(NDUFV2P1).The complex I enzyme activity microplate assay was carried out to evaluate the complex I activity induced by *** Herein,we reported decreasing levels of CPEB1 and NDUFV2 in schizophrenia *** studies showed that ERVWE1 was negatively correlated with CPEB1 and NDUFV2 in ***,NDUFV2P1 was increased and demonstrated a significant positive correlation with ERVWE1 and a negative correlation with NDUFV2 in *** vitro experiments disclosed that ERVWE1 suppressed NDUFV2 expression and promoter activity by increasing NDUFV2P1 *** luciferase assay revealed that ERVWE1 could enhance the promoter

关键词： ERVWE1 CPEB1 NADH dehydrogenase ubiquinone flavoprotein 2 complex I Pseudogene

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miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong Jing-Jing Hu yan Yu xiao-Li Li xiao-Ting Sun Li-Ting Wang xia Wu Ling-Jie Zhu xiao-Jia yang Qian-ru He Fei Ding Hai-yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

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CaH2-promoted activity of Ni-carbonate interface for CO2 methanation

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Journal of Energy Chemistry 2025年第1期100卷 522-532页

作者： Jin-Peng Wang Guo-Cui Mao Hui-Lin Jiang Bao-xia Dong Yun-Lei Teng School of Chemistry and Chemical Engineering Yangzhou University

Transition metal-carbonate interfaces often act as active sites in heterogeneous catalytic *** interface between transition metal and metal carbonate exhibits a dynamic equilibrium during the CO2hydrogenation reaction,involving surface carbonate hydrogenation and ***,there have been few reports on engineering the activity of the interface between transition metal and alkaline earth metal carbonate for catalytic *** work demonstrated that the incorporation of CaH2in Ni/CaCO3enhances the CO2methanation activity of the *** CO2conversion for Ni/CaH2-CaCO3reached 68.5%at 400°C,which was much higher than that of the Ni/CaCO3（31.6%） and Ni/CaH2-CaO （42.4%） ***,the Ni/CaH2-CaCO3catalysts remained stable during the stability test for 24 h at 400°C and 8 *** research revealed that CaH2played a crucial role in promoting the activity of the Ni-carbonate interface for ***2could modify the electronic structure of Ni and tune the structural properties of CaCO3to generate medium basic sites （OH groups）,which are favorable for the activation of H2and ***-situ Fourier transform infrared spectroscopy （FTIR） analysis combined with density functional theory calculations demonstrated that CO2activation occurs at the hydroxyl group （OH） on the CaH2-modified Ni-carbonate surface,leading to the formation of CO3H****,our study has confirmed that CO2methanation over the Ni/CaH2-CaCO3catalysts proceeds via the formate pathway.

关键词： Metal hydride Ni catalysts Carbonate Metal-carbonate interface

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非哺乳期乳腺炎患者外周血T淋巴细胞、免疫球蛋白及补体水平的变化

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中华乳腺病杂志（电子版） 2012年第5期6卷 17-24页

作者：夏亚茹陈红风叶媚娜陈莉颖鲍以嘉上海中医药大学附属龙华医院乳腺科上海200032

目的探讨非哺乳期乳腺炎患者外周血T淋巴细胞、免疫球蛋白及补体水平的变化情况。方法本研究纳入2010年3月至2011年4月间上海中医药大学附属龙华医院收治的72例非哺乳期乳腺炎患者(非哺乳期乳腺炎组)和30例健康体检者(正常对照组),检测... 详细信息

目的探讨非哺乳期乳腺炎患者外周血T淋巴细胞、免疫球蛋白及补体水平的变化情况。方法本研究纳入2010年3月至2011年4月间上海中医药大学附属龙华医院收治的72例非哺乳期乳腺炎患者(非哺乳期乳腺炎组)和30例健康体检者(正常对照组),检测其外周血T淋巴细胞(CD3+、CD4+、CD8+、CD56+CD16+)、免疫球蛋白(IgG、IgA、IgM)和补体(C3、C4、B因子)的水平。采用t检验比较非哺乳期乳腺炎组与正常对照组间各免疫指标的差异;用单因素方差分析比较不同发病年龄、不同生育情况、不同乳房炎性结块面积及有无先天性乳头畸形者与正常对照组间外周血T淋巴细胞、免疫球蛋白及补体水平的差异。结果非哺乳期乳腺炎患者CD3+T细胞、CD8+T细胞水平均低于正常对照组(P均2年发病组(P均2年发病组CD8+T细胞水平均低于正常对照组(P均2年发病组B因子水平高于正常对照组(P27.00cm2组CD3+T细胞和CD4+T细胞水平均低于炎性结块面积≤27.00cm2组和正常对照组(P均0.050)。结论非哺乳期乳腺炎患者存在着免疫功能紊乱,其紊乱程度与发病年龄、生育状况及乳房炎性结块面积有关系。

关键词：乳腺炎 T淋巴细胞免疫球蛋白类补体系统蛋白质类非哺乳期

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Beauty Restored xiamen presents a successful case of sustainable development of a bay city

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ChinAfrica 2025年第1期17卷 36-39页

作者： xia YUANYUAN xiamen

Riding along xiamen’s Xinglin Bay Marine Bicycle Lane feels like gliding between the sea and *** 2.6-km-long pathway,built above the sea,links two sides of the bay.

关键词： xiamen Marine sustainable

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IFT52 plays an essential role in sensory cilia formation and neuronal sensory function in Drosophila

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Insect Science 2023年第4期30卷 1081-1091页

作者： ya-Nan Hou Ying-Ying Zhang ya-ru Wang Zhi-Mao Wu Yun-xia Luan Qing Wei Center for Energy Metabolism and Reproduction Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina Shenzhen Key Laboratory of Metabolic Health ShenzhenGuangdong ProvinceChina Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education Institute of BiotechnologyShanxi UniversityTaiyuanChina Guangzhou Key Laboratory of Insect Development Regulation and Application Research Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouGuangdongChina

Cilia are microtubule-based,hair-like organelles involved in sensory function or motility,playing critical roles in many physiological processes such as reproduction,or-gan development,and sensory *** insects,cilia are restricted to certain sensory neurons and sperms,being important for chemical and mechanical sensing,and *** great progress has been made regarding the mechanism of cilia assembly,the formation of insect cilia remains poorly understand,even in the insect model organism *** transport(IFT)is a cilia-specific complex that traffics protein cargos bidirectionally along the ciliary axoneme and is essential for most *** we investigated the role of IFT52,a core component of IFT-B,in cilia/flagellar formation in *** show that Drosophila IFT52 is distributed along the sensory neuronal cilia,and is essential for sensory cilia *** of Ift52 results in severe defects in cilia-related sensory *** should be noted that IFT52 is not detected in sper-matocyte cilia or sperm flagella of ***,ift52 mutants can produce sperms with normal motility,supporting a dispensable role of IFT in Drosophila sperm flagella ***,IFT52 is a conserved protein essential for sensory cilia formation and sensory neuronal function in insects.

关键词： Drosophila IFT52 sensory cilia sperm flagella

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Potential dependence in electrocatalysis: a theoretical perspective

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Science China Chemistry 2025年第1期 21-25页

作者： Leyu Liu Zhaoming xia Zeyu Wang Yinjuan Chen Hai xiao Department of Chemistry Tsinghua University Fundamental Science Center of Rare Earths Ganjiang Innovation Academy Chinese Academy of Sciences Key Laboratory of Advanced Catalytic Materials and Technology Advanced Catalysis and Green Manufacturing Collaborative Innovation Center Changzhou University

Electrocatalysis is a key technology for the new energy era and sustainable development, serving as the interface for interconversion of electrical and chemical energies, which spans a wide spectrum of applications in sustainable energy and environmental engineering. Electrocatalytic reactions including electrochemical CO2reduction reaction （eCO2RR）, hydrogen evolution reaction （HER）, and oxygen evolution/reduction reactions （OER/ORR）, hold essential promise for renewable energy technologies as well as complement the biogeochemical carbon and oxygen cycles.

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Subretinal fibrosis secondary to neovascular age-related macular degeneration:mechanisms and potential therapeutic targets

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Neural Regeneration Research 2025年第2期20卷 378-393页

作者： Jingxiang Zhang xia Sheng Quanju Ding Yujun Wang Jiwei Zhao Jingfa Zhang Department of Ophthalmology People’s Hospital of Huangdao DistrictQingdaoShandong ProvinceChina Department of Urology People’s Hospital of Huangdao DistrictQingdaoShandong ProvinceChina Department of Ophthalmology Shanghai General Hospital(Shanghai First People’s Hospital)Shanghai Jiao Tong University School of MedicineShanghaiChina National Clinical Research Center for Eye Diseases Shanghai Key Laboratory of Ocular Fundus DiseasesShanghai Engineering Center for Visual Science and PhotomedicineShanghai Engineering Center for Precise Diagnosis and Treatment of Eye DiseasesShanghaiChina

Subretinal fibrosis is the end-stage sequelae of neovascular age-related macular *** causes local damage to photoreceptors,retinal pigment epithelium,and choroidal vessels,which leads to permanent central vision loss of patients with neovascular age-related macular *** pathogenesis of subretinal fibrosis is complex,and the underlying mechanisms are largely ***,there are no effective treatment options.A thorough understanding of the pathogenesis of subretinal fibrosis and its related mechanisms is important to elucidate its complications and explore potential *** current article reviews several aspects of subretinal fibrosis,including the current understanding on the relationship between neovascular age-related macular degeneration and subretinal fibrosis;multimodal imaging techniques for subretinal fibrosis;animal models for studying subretinal fibrosis;cellular and non-cellular constituents of subretinal fibrosis;pathophysiological mechanisms involved in subretinal fibrosis,such as aging,infiltration of macrophages,different sources of mesenchymal transition to myofibroblast,and activation of complement system and immune cells;and several key molecules and signaling pathways participating in the pathogenesis of subretinal fibrosis,such as vascular endothelial growth factor,connective tissue growth factor,fibroblast growth factor 2,platelet-derived growth factor and platelet-derived growth factor receptor-β,transforming growth factor-βsignaling pathway,Wnt signaling pathway,and the axis of heat shock protein 70-Toll-like receptors 2/*** review will improve the understanding of the pathogenesis of subretinal fibrosis,allow the discovery of molecular targets,and explore potential treatments for the management of subretinal fibrosis.

关键词： choroidal neovascularization epithelial-mesenchymal transition mesenchymal transition myofibroblast neovascular age-related macular degeneration submacular fibrosis subretinal fibrosis therapeutic targets transforming growth factor-β vascular endothelial growth factor

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Bullous pemphigoid associated with acquired hemophilia A: A case report

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World Journal of Clinical Cases 2025年第4期13卷 28-33页

作者： Su-Ye Hu Meng-Can Li Zi-Jia Hao Xu-ya Chai Pei-Sai Li yang Liu Li-xia Liu Ying Xu Pan-Pan yang Ling-E Li Department of Dermatology Shijiazhuang Traditional Chinese Medicine HospitalShijiazhuang 050051Hebei ProvinceChina Graduate School Hebei University of Traditional Chinese MedicineShijiazhuang 050051Hebei ProvinceChina Department of Pathology Shijiazhuang Traditional Chinese Medicine HospitalShijiazhuang 050051Hebei ProvinceChina

BACKGROUND Acquired hemophilia A (AHA) is a rare and potentially severe bleeding disordercaused by circulating autoantibodies against factor Ⅷ (FⅧ). In approximately50% of the patients, the condition is associated with autoimmune diseases,cancers, medication use, pregnancy, and the post-partum period. Bullous pemphigoid(BP) is a chronic autoimmune subepidermal blistering disease associatedwith tissue-bound and circulating autoantibodies against BP antigens 180 (BP180)and 230 (BP230). AHA-associated BP has a high mortality rate;hence, the understandingof this disease must *** SUMMARY A 69-year-old man presented with erythema, blisters, blood blisters, and crustsaccompanied by severe pruritus for more than 20 days, and ecchymosis andswelling on his left upper arm for 3 days. Pathological examination revealed asubepidermal blister that contained eosinophils. Laboratory tests showed that theBP180 autoantibody levels had increased, isolated activated partial thromboplastintime was notably prolonged (115.6 s), and coagulation FⅧ activity wasextremely low (< 1.0%). Furthermore, the FⅧ inhibitor titer had greatlyincreased (59.2 Bethesda units). Therefore, the patient was diagnosed as having BP associated with AHA, prescribed 0.05% topical halometasone cream, and transferred to a higher-level hospitalfor effective treatment;however, he died after 2 *** AHA associated BP is rare, dangerous, and has a high mortality rate. Therefore, its timely diagnosis and effectivetreatment are necessary.

关键词： Bullous pemphigoid Acquired hemophilia A Acquired hemophilia A FactorⅧ Case report

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