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A novel insight of enhancing the hydrogen peroxide tolerance of unspecific peroxygenase from Daldinia caldariorum based on structure

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Chinese Chemical Letters 2024年第4期35卷 426-430页

作者： Tiantian li Ruochen Jin Bin Wu Dongming lan Yunjian Ma Yonghua Wang School of Food Science and Engineering South China University of TechnologyGuangzhou 510640China School of Bioscience and Bioengineering South China University of TechnologyGuangzhou 510006China Guangdong Youmei Institute of Intelligent Bio-manufacturing Co. Ltd.Foshan 528200China

Unspecific peroxygenases(UPOs, EC 1.***.2.1) is a kind of thioheme enzyme capable of catalyzing various oxidations of inert C–H bonds using H_(2)O_(2) as an oxygen donor without cofactors. However, the enhancement of the H_(2)O_(2) tolerance of UPOs is always challenging. In this study, the A161C mutant of r Dca UPO,which originates from Daldinia caldariorum, was found to be highly H_(2)O_(2)-resistant. Compared with the wild type, the mutant r Dca UPO-A161C showed a 10-h prolonged half-life and a 64% improved enzyme activity when incubated in 10 mmol/L H_(2)O_(2). The crystal structure analysis at 1.47 A showed that r Dca UPOA161C exhibited 10 α-helixes(cyan) and a series of ordered rings, forming a single asymmetric spherical structure. The two conserved domains near heme formed an active site with the catalytic PCP and EHD regions(Glu86, His87, Asp88 residues). The H_(2)O_(2) tolerance of r Dca UPO-A161C was preliminarily explored by comparing its structure with the wild type. Notably, r Dca UPO-A161C showed significantly higher catalytic efficiency than the wild type for the production of hydroxyl fatty acids. This study is anticipated to provide an insight into the structure-function relationship and expand potential applications of UPOs.

关键词： Unspecific peroxygenase Molecular modification Hydrogen peroxide stability Crystal structure Hydroxyl fatty acids

Construction of ternary PEG200-based DESs lubrication systems via tailoring tribo-chemistry

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Friction 2024年第4期12卷 655-669页

作者： Yuting li Songyu lan yazhou liU Cheng CAO Zicheng TANG Deyin DENG Fuyuan liU Hao li Xiaoqiang FAN Minhao ZHU Key Laboratory of Advanced Technologies of Materials(Ministry of Education) School of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China Tribology Research Institute School of Mechanical EngineeringSouthwest Jiaotong UniversityChengdu 610031China

Designing novel lubricants with easily customized structures,devisable compositions,and simple and economic synthesis over traditional lubricants is critical to fulfilling complex applications,prolonging machine lifetime,and saving *** eutectic solvents(DESs),which show tunable composition,adjustable structure,easy fabrication,and environmental friendliness,are promising candidates for variable and complicated lubricants *** promote the use of DESs as lubricants,a series of PEG200-based DESs with active heteroatoms were fabricated to tailor the tribological performance via ***,PEG200/boric acid(BA)DES shows optimal lubrication performance by forming tribo-chemical reaction film composited of B2O3,iron oxides,and FeOOH,and PEG200/thiourea(TU)DES displays abrasive wear-reducing property by producing FeS tribo-chemical *** the excellent abrasive wear-resistance of PEG200/TU DES and friction reduction of PEG200/BA DES,ternary PEG200/BA/TU DESs,composited of PEG200/TU DES and PEG200/BA DES,are first *** ternary DESs possess superior wettability and thermal stability,which render them potential *** tests of the ternary DESs demonstrate that synergistic lubrication is achieved by forming a transfer film consisting of FexBy,BN,B2O3,and *** FexBy,BN,and B2O3 increase load bearing of the film,and FeS mitigates severe abrasive *** proposed design philosophy of novel DESs as lubricants opens up a unique realm that is unattainable by traditional DESs lubrication mechanisms and provides a platform to design next-generation DESs lubrication systems.

关键词： deep eutectic solvents(DESs) PEG200/boric acid DES PEG200/thiourea DES ternary DESs synergistic lubrication tribo-chemistry

Clinical nursing value of predictive nursing in reducing complications of pregnant women undergoing short-term massive blood transfusion during cesarean section

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World Journal of Clinical Cases 2024年第1期12卷 51-58页

作者： li Cheng li-Ping li Yuan-Yuan Zhang Fang Deng Ting-Ting lan Department of Obstertrics Wuhan Third HospitalWuhan 430000Hubei ProvinceChina Department of Gynaecology Wuhan No.1 HospitalWuHan 430030Hubei ProvinceChina Center for Reproductive Medicine Department of Obstetrics and GynecologySichuan Provincial People's HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengdu 610072Sichuan ProvinceChina

BACKGROUND Cesarean hemorrhage is one of the serious complications,and short-term massive blood transfusion can easily cause postoperative infection and physical stress ***,predictive nursing intervention has important clinical significance for *** To explore the effect of predictive nursing intervention on the stress response and complications of women undergoing short-term mass blood transfusion during cesarean section(CS).METHODS A clinical medical record of 100 pregnant women undergoing rapid mass blood transfusion during sections from June 2019 to June *** to the different nursing methods,patients divided into control group(n=50)and observation group(n=50).Among them,the control group implemented routine nursing,and the observation group implemented predictive nursing intervention based on the control ***,compared the differences in stress res-ponse,complications,and pain scores before and after the nursing of pregnant women undergoing rapid mass blood transfusion during *** The anxiety and depression scores of pregnant women in the two groups were significantly improved after nursing,and the psychological stress response of the observation group was significantly lower than that of the control group(Plivery were lower than those of the control group,and the MAP at the end of delivery was lower than that of the control group(Plications such as skin rash,urinary retention,chills,diarrhea,and anaphylactic shock in the observation group were 18%,which significantly higher than in the control group(4%)(Plieve the pain,reduce the incidence of complications,improve mood and stress response,and serve as a

关键词： Predictive care Rapid mass blood transfusion Cesarean section Stress response Complications

SUPER WOMAN 2(SPW2)maintains organ identity in spikelets by inhibiting the expression of floral homeotic genes OsMADS3,OsMADS58,OsMADS13,and DROOPING LEAF

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Journal of Integrative Agriculture 2024年第1期23卷 59-76页

作者： Hui Zhuang Jinsong lan Qiuni yang Xiaoyu Zhao Yuhuan li Jingya Zhi yalin Shen GuanghuaHe Yunfeng li Rice Research Institute Key Laboratory of Application and Safety Control of Genetically Modified CropsAcademy of Agricultural SciencesSouthwest UniversityChongqing 400715China

Flower organ identity in rice is mainly determined by the A-,B-,C-and E-class genes,with the majority encoding MADS-box transcription ***,few studies have investigated how the expression of these floral organ identity genes is regulated during flower *** this study,we identified a gene named SUPER WOMAN 2(SPW2),which is necessary for spikelet/floret development in rice by participating in the regulation of the expression of pistil identity genes such as OsMADS3,OsMADS13,OsMADS58 and *** the spw2 mutant,ectopic stigma/ovary-like tissues were observed in the non-pistil organs,including sterile lemma,lemma,palea,lodicule,and stamen,suggesting that the identities of these organs were severely affected by mutations in ***2 was shown to encode a plant-specific EMF1-like protein that is involved in H3K27me3 modification as an important component of the PRC2 *** analysis showed that the SPW2 mutation led to the ectopic expression of OsMADS3,OsMADS13,OsMADS58,and DL in non-pistil organs of the *** ChIP-qPCR results showed significant reductions in the levels of H3K27me3 modification on the chromatin of these ***,we demonstrated that SPW2 can mediate the process of H3K27me3 modification of pistil-related genes to regulate their expression in non-pistil organs of spikelets in *** results of this study expand our understanding of the molecular mechanism by which SPW2 regulates floral organ identity genes through epigenetic regulation.

关键词： rice(Oryza sativa) spikelet organ identity H3K27me3

Copper-based electro-catalytic nitrate reduction to ammonia from water:Mechanism,preparation,and research directions

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Environmental Science and Ecotechnology 2024年第4期20卷 42-62页

作者： Jinshan Wei Yi li Hexing lin Xihui Lu Chucheng Zhou ya-yun li Shenzhen Key Laboratory of Special Functional Materials&Shenzhen Engineering Laboratory for Advance Technology of Ceramics College of Materials Science and EngineeringShenzhen UniversityShenzhen518060PR China

Global water bodies are increasingly imperiled by nitrate pollution,primarily originating from industrial waste,agricultural runoffs,and urban *** escalating environmental crisis challenges traditional water treatment paradigms and necessitates innovative ***-catalysis,especially utilizing copper-based catalysts,known for their efficiency,cost-effectiveness,and eco-friendliness,offer a promising avenue for the electro-catalytic reduction of nitrate to *** this review,we systematically consolidate current research on diverse copper-based catalysts,including pure Cu,Cu alloys,oxides,single-atom entities,and ***,we assess their catalytic performance,operational mechanisms,and future research directions to find effective,long-term solutions to water purification and ammonia ***-catalysis technology shows the potential in mitigating nitrate pollution and has strategic importance in sustainable environmental *** to the application,challenges regarding complexity of the real water,the scale-up of the commerical catalysts,and the efficient collection of produced NH3 are still *** reseraches of catalyst specially on long term stability and in situ mechanisms are proposed.

关键词： Water treatment Nitrate removal Electrocatalysis Cu catalysts Ammonia synthesis

Interactions of phenol and benzaldehyde in electrocatalytic upgrading process

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Chinese Chemical Letters 2024年第2期35卷 464-468页

作者： lan liang Chuanbin Wang Xukai Lu Yunan Sun Beibei yan Ning li Guanyi Chen li'an Hou School of Environmental Science and Engineering Tianjin Key Lab of Biomass/Wastes UtilizationTianjin UniversityTianjin 300350China School of Mechanical Engineering Tianjin University of CommerceTianjin 300134China Georgia Tech Shenzhen Institute Tianjin UniversityShenzhen 518071China High Tech Inst Beijing Beijing 100085China

Biomass pyrolysis oil can be improved effectively by electrocatalytic hydrogenation(ECH).However,the unclear interactions among different components lead to low bio-oil upgrading efficiency in the conversion ***,benzaldehyde and phenol,as common compounds in bio-oil,were chosen as model *** interactions between the two components were explored in the ECH process by combining experiments and theoretical *** showed that phenol could accelerate the conversion of benzaldehyde in the *** selectivity of benzyl alcohol was increased from 60.9%of unadded phenol to 99.1%with 30 mmol/L phenol concentration at 5 *** inhibited the ECH of *** the presence of benzaldehyde,the conversion rate of phenol was below 10.0%with no cyclohexanone and cyclohexanol formation at 5 *** density functional theory(DFT)calculations revealed that the phenol could promote the adsorption of benzaldehyde and facilitate the targeted conversion of benzaldehyde on the active site by lowering the reaction energy *** research on the interaction between phenol and benzaldehyde in the ECH provides a theoretical basis for the application of ECH in practical bio-oil upgrading.

关键词： Interaction Bio-oil Electrocatalytic hydrogenation Phenol Benzaldehyde

Wigner function of optical cumulant operator and its dissipation in thermo-entangled state representation

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Chinese Physics B 2024年第6期33卷 205-210页

作者：张科李兰兰范洪义 School of Electronic Engineering Huainan Normal UniversityHuainan 232038China Department of Material Science and Engineering University of Science and Technology of ChinaHefei 230026China

To conveniently calculate the Wigner function of the optical cumulant operator and its dissipation evolution in a thermal environment, in this paper, the thermo-entangled state representation is introduced to derive the general evolution formula of the Wigner function, and its relation to Weyl correspondence is also discussed. The method of integration within the ordered product of operators is essential to our discussion.

关键词： Wigner function optical cumulant operator dissipation evolution thermo-entangled state representation integration within ordered product of operators

Development of a diagnostic nomogram for alpha-fetoproteinnegative hepatocellular carcinoma based on serological biomarkers

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World Journal of Gastrointestinal Oncology 2024年第6期16卷 2463-2475页

作者： li He Cui Zhang lan-lan liu li-Ping Huang Wen-Jing Lu Yuan-Yuan Zhang De-Yong Zou Yu-Fei Wang Qing Zhang Xiao-li yang School of Clinical Medicine Weifang Medical UniversityWeifang 261053Shandong ProvinceChina Department of Organ Transplantation The Third Medical Centre of Chinese PLA General HospitalBeijing 100039China Department of Laboratory Medicine The Third Medical Centre of Chinese PLA General HospitalBeijing 100039China Department of Laboratory Medicine Jingyu County People’s HospitalBaishan 135200Jilin ProvinceChina School of Laboratory Medicine Weifang Medical UniversityWeifang 261053Shandong ProvinceChina

BACKGROUND Hepatocellular carcinoma(HCC)is the third leading cause of cancer-related death *** biomarkers play an important role in the early diagnosis and prognosis of *** a certain percentage of HCC patients are negative for alpha-fetoprotein(AFP),the diagnosis of AFP-negative HCC is essential to improve the detection rate of *** To establish an effective model for diagnosing AFP-negative HCC based on serum tumour *** A total of 180 HCC patients were enrolled in this *** expression levels of GP73,des-γ-carboxyprothrombin(DCP),CK18-M65,and CK18-M30 were detected by a fully automated chemiluminescence *** variables were selected by logistic regression *** models were constructed using stepwise backward logistic *** performance of the models was compared using the C statistic,integrated discrimination improvement,net reclassification improvement,and calibration *** clinical utility of the nomogram was assessed using decision curve analysis(DCA).RESULTS The results showed that the expression levels of GP73,DCP,CK18-M65,and CK18-M30 were significantly greater in AFP-negative HCC patients than in healthy controls(Plity(area under the curve=0.946)and good goodness of *** DCA curves indicated the good clinical utility of the *** Our study identified GP73 and CK18-M65 as serum biomarkers with certain application value in the diagnosis of AFP-negative *** diagnostic nomogram based on CK18-M65 combined with GP73 demonstrated good performance and effectively identified high-risk groups of patients with HCC.

关键词： Hepatocellular carcinoma Alpha-fetoprotein-negative Diagnostic Nomogram Biomarker

Dynamic lithium-compensation mechanism for densification of garnet-type li_(7)La_(3)Zr_(2)O_(12)electrolyte by li_(2)O atmosphere buffer pair

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Nano Research 2024年第7期17卷 6184-6191页

作者： Chujun Zheng ya Chen Haoxin Dong yan Lu Jun Jin Zhaoyin Wen CAS Key Laboratory of Materials for Energy Conversion Shanghai Institute of CeramicsChinese Academy of ScienceShanghai 200050China State Key Lab High Performance Ceram and Superfine Shanghai Institute of CeramicsChinese Academy of SciencesShanghai 200050China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

Solid-state lithium metal batteries are one of the most promising options for next-generation batteries pursuing high-energy density and ***,the inevitable volatilization of lithium compounds during sintering leads to low relative density and low ionic conductivity of solid-state ***,the dynamic lithium-compensation mechanism is proposed to facilitate the densification of Ta-substituted garnet-type electrolyte(li_(6.5)La_(3)Zr_(1.5)Ta_(0.5)O_(12)(LLZT))through the reversible manipulating of li_(2)O ***_(2)ZrO_(3)is used as mother powder additive,which reacts with li_(2)O in sintering atmosphere and forms li_(6)Zr_(2)O_(7).li_(2)ZrO_(3)/li_(6)Zr_(2)O_(7)buffer pair manipulates the sintering li_(2)O atmosphere,which is vital for LLZT,within the li_(2)O partial pressure range corresponding to li_(2)ZrO_(3)and li_(6)Zr_(2)O_(7).Furthermore,the reversibility mechanism of buffer pair for li_(2)O absorption and release is *** obtained LLZT exhibits a relative density of over 96%and an ionic conductivity exceeding 7×10^(−4)S·cm^(−1)with no abnormal grain *** symmetric cell demonstrates an excellent lithium dendrite suppressing ability(stable cycling at a current density of 0.3 mA·cm^(−2)for over 1000 h).Such dynamic lithium-compensation strategy has been successfully applied in atmosphere manipulation of LLZT sintering process,which reduces the dependence of LLZT on the li_(2)O atmosphere,making it conducive to large-scale preparation of electrolyte ceramics.

关键词： solid-state batteries garnet-type electrolyte high-density electrolyte dynamic lithium-compensation buffer pair