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discrimination of foodborne pathogenic bacteria using synchrotron FTIR microspectroscopy

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Nuclear Science and Techniques 2017年第4期28卷 38-43页

作者： ya-di wang Xue-Ling Li Zhi-Xiao Liu Xing-Xing Zhang Jun Hu Jun-Hong Lü division of Physical Biology and CAS Key Laboratory of Interfacial Physics and Technology Shanghai Institute of Applied Physics Chinese Academy of Sciences (CAS)Shanghai 201800 China University of Chinese Academy of Sciences Beijing 100049China Shanghai Center for Bioinformation Technology Shanghai Academy of Science and Technology Shanghai 201203China Center for Clinical and Translational Medicine Shanghai Industrial Technology Institute Shanghai 201203 China

Traditional Fourier transform infrared(FTIR)spectroscopy has been recognized as a valuable method to characterize and classify kinds of *** this study,combined with multivariate statistical analysis,synchrotron radiation-based FTIR(SR-FTIR) microspectroscopy was applied to identify and discriminate ten foodborne bacterial *** results show that the whole spectra(3000-900 cm^(-1)) and three subdivided spectral regions(3000-2800,1800-1500 and 1200-900 cm^(-1),representing lipids,proteins and polysaccharides,respectively) can be used to type *** the whole spectra or the three subdivided spectra are good for discriminating the bacteria at levels of species and subspecies,but the whole spectra should be given preference at the genus *** findings demonstrate that SR-FTIR microspectroscopy is a powerful tool to identify and classify foodborne pathogenic bacteria at the genus,species and subspecies level.

关键词： Synchrotron FTIR microspectroscopy Foodborne pathogens Bacterial discrimination Subdivided spectral regions Multivariate statistical analysis

Anti-corrosive and highly reversible zinc metal anode enabled by the phenolic resin coating

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Rare Metals 2024年第5期43卷 2115-2124页

作者： ya-Ping wang ya-di yang Shu-Qi Duan Li wang Jia-Shuo Guo An-Qiang Pan School of Materials Science and Engineering Hebei University of TechnologyTianjin 300130China Key Laboratory of Special Functional Materials for Ecological Environment and Information(Hebei University of Technology).Ministry of Education Tianjin 300130China Key Laboratory for New Type of Functional Materials in Hebei Province Hebei University of TechnologyTianjin 300130China School of Materials Science and Engineering Central South UniversityChangsha 410083China

Aqueous zinc-ion batteries have attracted much attention due to their high theoretical capacity,low cost high safety,and ***,challenges such as dendrite growth and side reactions severely hinder the electrochemical performance of the Zn anode,leading to low Coulombic efficiency(CE)or even short circuits Herein,phenolic resin(PF)is used as a protective coating on the surface of the zinc metal *** PF layer can greatly improve the corrosion resistance of the Zn electrode in the ***,this artificial electrode/electrolyte interphase also elevates the nucleation barrier and restrains Zn^(2+)2D diffusion,regulating the zinc deposition/dissolution *** a result,PF@Zn symmetric cells exhibit superior performance than that of bare Zn symmetric cells,achieving a stable and dendrite-free cycle of 1400 ***,the carbon nanotube MnO_(2)||PF@Zn(CNT/MnO_(2)||PF@Zn)full cell also delivers a long cycle life with a high-capacity retention of about 180.0 m Ah·g^(-1)after 1400 cycles,far exceeding that of bare Zn *** work provides a facile strategy for achieving a dendrite-free and rechargeable zinc anode.

关键词： Rechargeable aqueous batteries Zn metal anode Phenolic resin Anti-corrosion

Synchrotron FTIR spectroscopy reveals molecular changes in Escherichia coli upon Cu^2＋ exposure

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Nuclear Science and Techniques 2016年第3期27卷 45-52页

作者： Xiao-Juan Hu Zhi-Xiao Liu ya-di wang Xue-Ling Li Jun Hu Jun-Hong Lv division of Physical Biology and CAS Key Laboratory of Interfacial Physics and Technology Shanghai Institute of Applied PhysicsChinese Academy of SciencesShanghai 201800China University of Chinese Academy of Sciences Beijing 100049China Shanghai Center for Bioinformation Technology Shanghai Academy of Science and TechnologyShanghai 201203China Center for Clinical and Translational Medicine Shanghai Industrial Technology InstituteShanghai 201203China

Copper ions(e.g.,Cu^(2+)) have outstanding antibacterial properties,but the exact mechanism is rather complex and not fully *** this work,synchrotron Fourier transform infrared(FTIR) spectroscopy was used as an analytical tool to investigate the CuCl_2-induced biochemical changes in Escherichia *** spectral measurements indicated that this technique is sensitive enough to detect changes in membrane lipids,nucleic acids,peptidoglycans and proteins of Cu^(2+)-treated ***,for short-time treated cells,the effects on phospholipid composition were clearly shown,while no significant alterations of proteins,nucleic acids and peptidoglycans were *** quantitative nano-mechanics mode atomic force microscopy(AFM)confirmed the changes in the topography and mechanical properties of bacteria upon the Cu^(2+) *** study demonstrated that FTIR spectroscopy combined with AFM can provide more comprehensive evaluation on the biochemical and mechanical responses of bacteria to copper.

关键词： CuCl2 红外光谱大肠杆菌同步原子力显微镜 FTIR光谱分子曝光

New insight of obesity-associated NAFLD: Dysregulated “crosstalk” between multi-organ and the liver?

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Genes & diseases 2023年第3期10卷 799-812页

作者： ya-di wang Liang-Liang Wu Xiao-yan Qi Yuan-Yuan wang Zhe-Zhen Liao Jiang-Hua Liu Xin-Hua Xiao Department of Metabolism and Endocrinology The First Affiliated HospitalHengyang Medical SchoolUniversity of South ChinaHengyangHunan 421001China Department of Gastrointestinal Surgery The First Affiliated HospitalHengyang Medical SchoolUniversity of South ChinaHengyangHunan 421001China

Obesity plays a crucial role in the development of non-alcoholic fatty liver disease (NAFLD). However, the underlying mechanism for the pathogenesis of obesity-associated NAFLD remains largely obscure. Although the “multiple hit” theory provides a more accurate explanation of NAFLD pathogenesis, it still cannot fully explain precisely how obesity causes NAFLD. The liver is the key integrator of the body's energy needs, receiving input from multiple metabolically active organs. Thus, recent studies have advocated the “multiple crosstalk” hypothesis, highlighting that obesity-related hepatic steatosis may be the result of dysregulated “crosstalk” among multiple extra-hepatic organs and the liver in obesity. A wide variety of circulating endocrine hormones work together to orchestrate this “crosstalk”. Of note, with deepening understanding of the endocrine system, the perception of hormones has gradually risen from the narrow sense (i.e. traditional hormones) to the broad sense of hormones as organokines and exosomes. In this review, we focus on the perspective of organic endocrine hormones (organokines) and molecular endocrine hormones (exosomes), summarizing systematically how the two types of new hormones mediate the dialogue between extra-hepatic organs and liver in the pathogenesis of obesity-related NAFLD.

关键词： Exosomes Hormones Non-alcoholic fatty liver disease(NAFLD) Obesity Organolkines

Epithelial membrane protein 1 negatively regulates cell growth and metastasis in colorectal carcinoma

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World Journal of Gastroenterology 2014年第14期20卷 4001-4010页

作者： Guo-Gui Sun ya-di wang Da-Wei Cui Yun-Jie Cheng Wan-Ning Hu Department of Chemoradiotherapy Tangshan People’s Hospital Department of Radiotherapy the Military General Hospital of Beijing PLA Department of Urology Tangshan Workers Hospital Department of Radiotherapy The Fourth Hospital of Hebei Medical University

AIM: To determine the expression and function of epithelial membrane protein 1 (EMP1) in colorectal carcinoma.

关键词： Epithelial membrane protein 1 Colorectal carcinoma Caspase-9 Vascular endothelial growth factor C Prognosis

Novel self-assembled porous yolk-shell NiO nanospheres with excellent electrochromic performance for smart windows

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Particuology 2024年第1期84卷 72-80页

作者： Baoshun wang ya Huang Siming Zhao Run Li di Gao Hairong Jiang Rufan Zhang Beijing Key Laboratory of Green Chemical Reaction Engineering and Technology Department of Chemical EngineeringTsinghua UniversityBeijing100084China

With the rapid development of optoelectronics,electrochromic(EC)materials(ECMs)with the advan-tages of low power consumption,easy viewing,high contrast ratio,etc have attached more and more attention from the fields of smart windows,electronic billboards,emerging wearable and portable electronics,and other next-generation *** oxide(NiO)is a promising candidate for high-performance ECMs because of its neutral-colored states and low ***,NiO-based ECMs still face the problem of slow switching speed due to their low electrical conductivity and small lattice ***-organic frameworks(MOFs)are promising candidates to fabricate hollow and porous transition metal oxides(TMOs)with high ion transport efficiency,excellent specific capacitance,and electrochemical *** this work,porous yolk-shell NiO nanospheres(PYS-NiO NSs)were syn-thesized via a solvothermal and subsequent calcination process of Ni-MOF,which exhibited outstanding EC *** the large specific surface areas and hollow porous nanostructures were conducive to ionic transport,PYS-NiO NSs exhibited a fast coloring/bleaching speed(3.6/3.9 s per one coloring/bleaching cycle)and excellent cycling stability(82%of capacity retention after 3000 cycles).These superior EC properties indicated that the PYS-NiO NSs was a promising candidate for high-performance EC *** work provides a new and feasible strategy for the efficient preparation of TMOs ECMs with good EC performance,especially fast switching speed.

关键词： Electrochromic Nickel oxide Metal-organic frameworks Hollow structure Smart windows

Effects on Proliferation and Migration of the Human Colon Carcinoma Cell Line SW620 by Silencing of Hepatocyte Growth Factor Expression

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Clinical oncology and cancer researeh 2010年第5期7卷 277-283页

作者： Yi-tao JIA Lei ZHANG yan LI ya-di wang Wei GUO Lei CAO Zhong-xin LI 3rd Department of Oncology Hebei ProvincialPepeol's Hospital Shijiazhuang 050051 HebeiProvince China. Department of Histology and Embryology Hebei Medical University Shijiazhuang050017 Hebei Province China Department of Radiotherapy Hebei Medical University Fourth Hospital Shijiazhuang050011 Hebei Province China. 2nd Department of Surgery Hebei Medical University Foruth Hospital Shijiazhuang050011 Hebei Province China.

OBJECTIVE Hepatocyte growth factor （HGF） expression is closely related to the progression and poor prognosis of colorectal cancer patients. In this study, we investigated the effects on proliferation and migration of the human colon carcinoma cell line SW620 by silencing HGF expression. METHODS HGF was silenced using specific HGF a/f3 siRNA. The proliferation, migration, cell cycle and ultrastructure of SW620 cells were examined. RESULTS The transfection efficiency was 70%-80%. The expression rate of HGF in the experimental group was significantly lower than that in the negative and blank control groups （P 〈 0.05）. The proliferation inhibition rate in the experimental group at 24, 48, 72 and 96 h after transfection was 14.2%, 50.2%, 39.5% and 23.2%, respectively. The migratory ability of cells in the experimental group was significantly inhibited compared with that in the negative control or blank control groups （58.2% vs. 2.1% or 0%, P 〈 0.05）. CONCLUSION The application of RNA interference to silence the expression of HGF in the colon carcinoma cell line SW620 effectively inhibits the proliferation and migration of tumor cells.

关键词： RNA interference HGF protein human cellular proliferation cell movement colorectal neoplasms.

SnS_(2) nanosheets decorated SnO_(2) hollow multishelled nanostructures for enhanced sensing of triethylamine gas

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Rare Metals 2024年第5期43卷 2339-2348页

作者： Wen-di Liu ya Xiong Ao Shen Xin-Zhen wang Xiao Chang Wen-Bo Lu Jian Tian School of Materials Science and Engineering Shandong University of Science and TechnologyQingdao 266590China College of Physics Qingdao UniversityQingdao 266071China Zernike Institute for Advanced Materials University of Groningen9747 AG GroningenThe Netherlands

Highly sensitive,fast and low-temperature detection of triethylamine(TEA)gas based on SnO_(2) is attractive yet remains ***,SnS_(2) nanosheets(NSs)/SnO_(2) hollow multishelled structures(HoMSs)-based sensors are designed by the synthesis of SnO_(2) HoMSs,followed by in situ sulfuration with *** varying the thioacetamide levels,Sn S_(2)/SnO_(2) heterostructures with different SnS_(2) contents were obtained and their TEA gas sensing performances were investigated.

关键词： SnS attractive sheets

One more step to realistic CO_(2) reduction reactions——geometric structures evolutions at gas-solid interface

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Rare Metals 2024年第2期43卷 849-852页

作者： ya-Qiang Xie Xin Yu Ting wang College of the Environment and Ecology Xiamen UniversityXiamen361102China

A rational design of copper(Cu)nanocatalyst is critical to the efficient carbon dioxide(CO_(2))conversion toward C1/C2 chemical ***,the lack of actual information on the vicinal Cu interfaces hinders the intrinsic activitystructure correlation *** with atomic-scale in situ characterizations(AP-STM and synchrotron-based AP-XPS),Park and co-workers study and identify the adsorbate-driven morphological evolution of Cu nanoclusters on the Cu(997)surface,providing real-space evidence for the critical role of vicinal structures on Cu *** observed surface roughening trends caused by dissociated atomic oxygen and CO adsorbates subsequently affect the result of successive catalytic reactions to produce critical intermediates,such as carbonate and formate,during the CO_(2)reduction reaction.

关键词： reaction critical rational