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Cotton functional genomics reveals global insight into genome evolution and fiber development

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Journal of Genetics and Genomics 2017年第11期44卷 511-518页

作者： Zhiguo Wu Yan Yang Gai Huang Jing Lin yuying xia Yuxian Zhu College of Life Sciences Wuhan UniversityWuhan 430072China Institute for Advanced Studies Wuhan UniversityWuhan 430072China State Key Laboratory of Protein and Plant Gene Research College of Life SciencesPeking UniversityBeijing 100871China

Due to the economic value of natural textile fiber, cotton has attracted much research attention, which has led to the publication of two diploid genomes and two tetraploid genomes. These big data facilitate functional genomic study in cotton, and allow researchers to investigate cotton genome structure, gene expression, and protein function on the global scale using high-throughput methods. In this review, we summarized recent studies of cotton genomes. Population genomic analyses revealed the domestication history of cultivated upland cotton and the roles of transposable elements in cotton genome *** splicing of cotton transcriptomes was evaluated genome-widely. Several important gene families like MYC, NAC, Sus and GhPLDal were systematically identified and classified based on genetic structure and biological function. High-throughput proteomics also unraveled the key functional proteins correlated with fiber development. Functional genomic studies have provided unprecedented insights into global-scale methods for cotton research.

关键词： Cotton Functional genomics Genome evolution Fiber development

Study on Contact Fatigue Life and Failure Mechanism of Subquenched 42CrMo Steel

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材料热处理学报 2004年第5期25卷 260-262页

作者： HEBo-lin yuying-xia SHAOEr-yu SchoolofMechanical＆ElectricalEngineering EastChinaJiaotongUniversityNanchangChina MaterialInstitute HenanUniversityofScienceandTechnologyLuoyangChina

The effect of undissolved ferrite amount in subcritically quenched 42CrMo steel on contact fatigue properties and failure mechanism were studied. The amount of undissolved ferrite in the steel were 0%,3%,10%,15% and 20% in volume fraction, respectively. The experimental results show that the existence of undissolved ferrite can increase the contact fatigue life The contact fatigue life can be prolonged with increasing the amounts of undissolved ferrite The grain size can be fined by using subcritical quenching process and the area of phase boundaries can also be greatly increased. The stress relaxation and grain refinement due to occurring of plastic deformation are main reasons for improving the fatigue life. The existence of undissolved ferrite can increase the crack initiation period. Under the experiment conditions, when the amount of undissolved ferrite is 10%, the longest contact fatigue life can be the obtained.

关键词：亚临界灭弧接触疲劳失效机理铁素体 42CrMo钢

Exploration of cyclooxygenase-2 inhibitory peptides from walnut dreg proteins based on in silico and in vitro analysis

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Food Science and Human Wellness 2024年第3期13卷 1636-1644页

作者： Zishan Hong Jing Xie Liang Tao Jing-Jing Dai Tingting Li Li Zhang yuying Bai xia Hu Jinlian Chen Jun Sheng Yang Tian College of Food Science and Technology Yunnan Agricultural UniversityKunming 650201China Yunnan Provincial Key Laboratory of Precision Nutrition and Personalized Food Manufacturing Yunnan Agricultural UniversityKunming 650201China Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources Ministry of EducationYunnan Agricultural UniversityKunming 650201China School of Tea and Coffee Pu’er UniversityPu’er 665000China Yunnan Provincial Engineering Research Center for Edible and Medicinal Homologous Functional Food Yunnan Agricultural UniversityKunming 650201China Key Laboratory of Pu-er Tea Science Ministry of EducationYunnan Agricultural UniversityKunming 650201China

Walnut dreg protein hydrolysates(WDPHs)exhibit a variety of biological activities,however,the cyclooxygenase-2(COX-2)inhibitory peptide of WDPHs remain *** aim of this study was to rapidly screen for such peptides in WDPHs through a combination of in silico and in vitro *** total,1262 peptide sequences were observed by nano liquid chromatography/tandem mass spectrometry(nano LC-MS/MS)and 4 novel COX-2 inhibitory peptides(AGFP,FPGA,LFPD,and VGFP)were *** kinetic data indicated that AGFP,FPGA,and LFPD displayed mixed-type COX-2 inhibition,whereas VGFP was a non-competitive *** is mainly because the peptides form hydrogen bonds and hydrophobic interactions with residues in the COX-2 active *** results demonstrate that computer analysis combined with in vitro evaluation allows for rapid screening of COX-2 inhibitory peptides in walnut protein dregs.

关键词： Walnut dreg proteins Cyclooxygenase-2 inhibitory peptide Identification Virtual screening Molecular docking

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磷酸盐水泥碳纤维复合材料的导电性能研究

功能材料 2016年第12期47卷 12124-12128页

作者：易峰丁铸夏玉莹深圳大学土木工程学院广东省滨海土木工程耐久性重点实验室广东深圳518060

以高掺量粉煤灰磷酸盐水泥为基体,短切碳纤维为功能材料,制备出了磷酸盐水泥碳纤维复合材料(PCFC),同时制备了碳纤维硅酸盐基复合材料(CFRC),并利用压力试验机、交流电桥和SEM对二者的基本力学性能、导电性能及内部碳纤维分布进行了对... 详细信息

以高掺量粉煤灰磷酸盐水泥为基体,短切碳纤维为功能材料,制备出了磷酸盐水泥碳纤维复合材料(PCFC),同时制备了碳纤维硅酸盐基复合材料(CFRC),并利用压力试验机、交流电桥和SEM对二者的基本力学性能、导电性能及内部碳纤维分布进行了对比研究。此外还考察了碳纤维掺量、龄期对PCFC导电性能的影响。结果表明,PCFC相比CFRC在同等碳纤维掺量的情况下力学增强效果更好、导电性能更为优越;机理分析表明PCFC中存在二次"渗流"现象,而CFRC试体中则没有。SEM照片显示碳纤维掺量较高时,PCFC中的碳纤维分散得更好;龄期实验结果表明,当碳纤维掺量超过1.4%时,龄期对PCFC的导电性影响较小,具有良好的导电稳定性。

关键词：碳纤维磷酸盐水泥导电性能二极法

低氧胁迫下虾夷扇贝的行为特征及生理生化响应

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中国水产科学 2021年第10期28卷 1319-1328页

作者：夏玉莹张继红刘毅上海海洋大学水产与生命学院中国水产科学研究院黄海水产研究所农业农村部海洋渔业可持续发展重点实验室

为研究低氧胁迫对虾夷扇贝(Patinopectenyessoensis)行为、生理生化(免疫防疫功能及关键呼吸酶)的影响,设置了1 mg/L、2 mg/L、4 mg/L、7 mg/L (对照组) 4个溶解氧梯度,测定分析了虾夷扇贝行为特征(外壳的开闭合程度大小)、耗氧率、排... 详细信息

为研究低氧胁迫对虾夷扇贝(Patinopectenyessoensis)行为、生理生化(免疫防疫功能及关键呼吸酶)的影响,设置了1 mg/L、2 mg/L、4 mg/L、7 mg/L (对照组) 4个溶解氧梯度,测定分析了虾夷扇贝行为特征(外壳的开闭合程度大小)、耗氧率、排氨率、抗氧化酶[超氧化物歧化酶(SOD),过氧化氢酶(CAT)]和呼吸相关酶[乳酸脱氢酶(LDH)和丙酮酸激酶(PK)]活性的响应情况。结果发现:(1)虾夷扇贝的存活率随着DO浓度的降低而降低, DO=1 mg/L时的存活率仅为55%;在DO=1 mg/L, 2 mg/L和4 mg/L时,虾夷扇贝的半致死时间分别为95.97 h、147.37 h和209.58 h。(2)将扇贝行为特征划分为5个等级,按照0～4赋分,评分越高,代表扇贝状态越好。从扇贝行为特性的量化指标来看, DO浓度越低,评分分数越低,虾夷扇贝状态越差。(3)低氧胁迫对虾夷扇贝耗氧率、排氨率有显著影响(P<0.05),在DO≤2 mg/L下,氧氮摩尔比<7,虾夷扇贝主要由蛋白质供能; DO≥4 mg/L,虾夷扇贝由蛋白质和脂肪氧化供能为主。(4)低氧胁迫对虾夷扇贝SOD、CAT和呼吸酶有显著性影响(P<0.05)。24 h的低氧胁迫使得肝胰腺及闭壳肌的自由基ROS浓度升高; 48～96 h的低氧胁迫下, SOD、CAT酶活开始降低。不同的溶解氧浓度下代谢途径不同, DO=2 mg/L时,有氧呼吸代谢转变为葡萄糖-丙酮酸-乳酸的呼吸途径; DO=1 mg/L时,呼吸代谢途径可能优先选择葡萄糖-琥珀酸途径。从生理生化层次上来看,免疫功能下降和呼吸代谢途径改变可能会引起虾夷扇贝行为特征改变。

关键词：虾夷扇贝低氧生理抗氧化酶呼吸关键酶

Perilipin 5 regulates hepatic stellate cell activation and high-fat diet-induced non-alcoholic fatty liver disease

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Animal Models and Experimental Medicine 2024年第2期7卷 166-178页

作者： Xuecui Yin Lin Dong xiaohan Wang Zhenzhen Qin yuying Ma xiaofei Ke Ya Li Qingde Wang Yang Mi Quanjun Lyu xia Xu Pengyuan Zheng Youcai Tang Department of Internal Medicine the Fifth Affiliated Hospital of Zhengzhou UniversityZhengzhouChina Department of Pediatrics the Fifth Affiliated Hospital of Zhengzhou UniversityZhengzhouChina Department of Clinical Nutrition the First Affiliated Hospital of Zhengzhou UniversityZhengzhouChina Key Laboratory of Advanced Drug Preparation Technologies Ministry of Education of ChinaCo-innovation Center of Henan Province for New drug R&D and Preclinical SafetySchool of Pharmaceutical SciencesZhengzhou UniversityZhengzhouChina Department of Pediatrics GastroenterologyHenan Key Laboratory of Rehabilitation MedicineHenan Joint International Research Laboratory of Chronic Liver Injury and Henan Provincial Outstanding Overseas Scientists Chronic Liver Injury Studiothe Fifth Affiliated Hospital of Zhengzhou UniversityZhengzhouChina

Background:Nonalcoholic fatty liver disease(NAFLD)is one of the most common chronic liver diseases *** stellate cells(HSCs)are the major effector cells of liver *** contain abundant lipid droplets(LDs)in their cytoplasm during *** 5(PLIN 5)is a LD surface-associated protein that plays a crucial role in lipid ***,little is known about the role of PLIN 5 in HSC ***:PLIN 5 was overexpressed in HSCs of Sprague–Dawley rats by lentivirus *** the same time,PLIN 5 gene knockout mice were constructed and fed with a high-fat diet(HFD)for 20 weeks to study the role of PLIN 5 in *** corresponding reagent kits were used to measure TG,GSH,Caspase 3 activity,ATP level,and mitochondrial DNA copy *** analysis of mice liver tissue metabolism was performed based on UPLC-MS/***,mitochondrial function,cell proliferation,and apoptosis-related genes and proteins were detected by western blotting and ***:Overexpression of PLIN 5 in activated HSCs led to a decrease in ATP levels in mitochondria,inhibition of cell proliferation,and a significant increase in cell apoptosis through AMPK *** addition,compared with the HFD-fed C57BL/6J mice,PLIN 5 knockout mice fed with HFD showed reduced liver fat deposition,decreased LD abundance and size,and reduced liver ***:These findings highlight the unique regulatory role of PLIN 5 in HSCs and the role of PLIN 5 in the fibrosis process of NAFLD.

关键词： AMPK apoptosis hepatic stellate cell liver fibrosis perilipin 5

Enteromorpha prolifera polysaccharide-Fe(Ⅲ) complex promotes intestinal development as a new iron supplement

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Science China Life Sciences 2024年

作者： Yingying Feng yuying Wu Jialu Wang Zhenglin Dong Qian Yu Shuangshuang xia Chunxue Liu Haihua Wang Xin Wu Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences CAS Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical Agriculture Chinese Academy of Sciences ANYOU Biotechnology Group Co.Ltd Qingdao Seawin Biotech Group Co. Ltd

Iron is a crucial micronutrient, and its deficiency can have detrimental effects on the health of infants. Dietary polysaccharide-iron(Ⅲ) complexes(PICs) are promising for addressing iron deficiency due to their minimal adverse reactions and high iron absorption rate. This study aimed to investigate the effects of dietary Enteromorpha prolifera polysaccharide-Fe(Ⅲ) complex(EP-Fe) on newborns, using 3-day weaned piglets as the iron-deficiency model. Results showed that EP-Fe improved iron levels and promoted intestinal development in piglets. Transcriptome sequencing revealed that EP-Fe increased the survival of intestinal epithelial cells under hypoxia by upregulating the expression of genes that promote the development of the vascular system. Additionally, EP-Fe enhanced the mucosal barrier functions by inhibiting myosin light chain kinase(MLCK)/phosphorylated myosin light chain(p-MLC)signaling pathway to increase the expression of intestinal tight junction proteins. Furthermore, the 16S rRNA gene sequencing of gut microbiota showed that EP-Fe promoted the enrichment of Bacteroides＿fragilis and other gut microbes that can metabolize carbohydrates. In conclusion, EP-Fe is an effective iron supplement for newborns, and it can be developed as a comprehensive nutritional supplement.

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Comparison of Salt Tolerance in Brassicas and Some Related Species

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American Journal of Plant Sciences 2013年第10期4卷 1911-1917页

作者： Jianjie Su Shu Wu Zhijie Xu Si Qiu Tingting Luo Yimin Yang Qitao Chen yuying xia Song Zou Bang-Lian Huang Bangquan Huang College of Life Science Hubei University Wuhan China

In this paper the salt tolerance in Brassicas and some related species was compared. When seedlings germinated on sand cultures with liquid MS medium were considered, the relative germination rate, root length, shoot length and fresh seedling weight were significantly correlated with each other (P 0.01), and only the relative shoot lengths were significantly different among the tested genotypes (P 0.05);When both seedlings germinated on MS and MS plus 0.4% NaCl were considered, only the relative shoot length of seedlings germinated on MS was significantly different from that germinated on MS + 0.4% NaCl (P 0.05), and also only the relative shoot lengths were significantly different among the tested genotypes (P 0.01). Raphanus sativa cv. Changfeng, B. juncea cv. JC and Brassica napus cv. ZS 10 showed low salt tolerance in terms of relative germination rate, root length, shoot length and fresh seedling weight;B. oleracea cv. JF-1, Sinapis alba cv. HN-2 showed high salt tolerance in terms of relative germination rate, root length, shoot length and fresh seedling weight. Based on our result we suggest that relative shoot length might be convenient to rank the salt tolerance but cluster analysis based on multiple parameters of relative germination rate, root length, shoot length and fresh seedling weight might be more accurate in screening for salt tolerance in Brassicas and related species.

关键词： Brassica Related Species Salt Tolerance Multivariate Cluster Analysis

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缺失数据建模的改进型ALS在线推荐算法

计算机工程 2018年第8期44卷 212-217,223页

作者：邢玉莹夏鸿斌王涵江南大学数字媒体学院江苏无锡214122

在隐式反馈中存在数据噪声并缺乏负反馈,使用矩阵分解方法训练推荐模型时特征提取不明确且推荐结果有偏斜。为此,提出一种缺失数据建模的改进型交替最小二乘(ALS)矩阵分解在线推荐算法。使用近邻信息为用户选择正样本,同时根据物品流行... 详细信息

在隐式反馈中存在数据噪声并缺乏负反馈,使用矩阵分解方法训练推荐模型时特征提取不明确且推荐结果有偏斜。为此,提出一种缺失数据建模的改进型交替最小二乘(ALS)矩阵分解在线推荐算法。使用近邻信息为用户选择正样本,同时根据物品流行度对缺失数据中的负样本进行建模,并将基于元素的改进型ALS算法与在线学习相结合。在MovieLens数据集上的实验结果表明,相对eALS、Rcd算法,该算法能够有效减小数据噪声和矩阵稀疏对矩阵分解推荐算法的影响,从而提高推荐的准确性和效率。

关键词：推荐系统隐式反馈缺失数据建模交替最小二乘在线学习