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Science China(Life Sciences) 2008年第2期51卷 133-139页

作者： yutaka banno Hiroshi FUJII Institute of Genetic Resource Faculty of AgricultureKyushu University

The chromosomal locations of two single-copy genes, Ser-1 and CI-13, in silkworm (Bombyx mori) were detected at the molecular cytogenetics level by fluorescence in situ hybridization in the study. The results showed that Ser-1 is located near the distal end of the 11th linkage group, relatively at the 12.5±1.4 position in pachytene; and that CI-13 has been mapped near the distal end of the 2nd linkage group, relatively at the 8.2±1.2 position in pachytene. Furthermore, their location model map-FISH map on silkworm chromosome was drawn. The FISH technique and its application to silkworm are also discussed in this paper.

关键词： Bombyx mori sericin gene 1(Ser-1) chymotrypsin inhibitor 13 gene (CI-13) fluorescence in situ hybridization chromosome mapping

Phylogeny and evolutionary history of the silkworm

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Science China(Life Sciences) 2012年第6期55卷 483-496页

作者： SUN Wei YU HongSong SHEN YiHong banno yutaka XIANG ZhongHuai ZHANG Ze Institute of Sericulture and Systems Biology Southwest UniversityChongqing 400715China Institute of Genetic Resources Graduate School of Bioresource and Bioenvironmental SciencesKyushu University Fukuoka 812-8581Japan Institute of Agricultural and Life Sciences Chongqing UniversityChongqing 400044China

The silkworm,Bombyx mori,played an important role in the old Silk Road that connected ancient Asia and ***,to date,there have been few studies of the origins and domestication of this species using molecular *** this study,DNA sequences of mitochondrial and nuclear loci were used to infer the phylogeny and evolutionary history of the domesticated silkworm and its *** of the phylogenetic analyses indicated a close relationship between the domesticated silkworm and the Chinese wild *** was estimated to have occurred about 4100 years ago(ya),and the radiation of the different geographic strains of *** about 2000 *** Chinese wild silkworm and the Japanese wild silkworm split about 23600 *** estimates are in good agreement with the fossil evidence and historical *** addition,we show that the domesticated silkworm experienced a population expansion around 1000 *** divergence times and the population dynamics of silkworms presented in this study will be useful for studies of lepidopteran phylogenetics,in the genetic analysis of domestic animals,and for understanding the spread of human civilizations.

关键词：系统发育分析进化史家蚕分子生物学方法中国野桑蚕 DNA序列昆虫系统学丝绸之路

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家蚕肾脏形卵(ki)的荧光差异显示研究

农业生物技术学报 2002年第3期10卷 262-266页

作者：李斌,夏庆友,Hiroshi Fujii,yutaka banno,鲁成西南农业大学农业部蚕桑学重点开放实验室西南农业大学农业部蚕桑学重点开放实验室 Institute of Genetic Resources Faculty of Agriculture Kyushu University Institute of Genetic Resources Faculty of Agriculture Kyushu University 西南农业大学农业部蚕桑学重点开放实验室重庆400716 重庆400716 6-10-1 Hakozaki Higashi-ku Fukuoka 812-8581 Japan 6-10-1 Hakozaki Higashi-ku Fukuoka 812-8581 Japan 重庆400716

为了探明家蚕(Bombyxmori )肾脏形卵的胚子不能正常发育的原因,使用FDD(flourescentdifferentdisplay)法对ki(kidney)基因同质型与异质型的母蛾所产未受精卵及受精24h卵的mRNA进行了差异显示研究。通过137组引物组合进行FDD差异显示,... 详细信息

为了探明家蚕(Bombyxmori )肾脏形卵的胚子不能正常发育的原因,使用FDD(flourescentdifferentdisplay)法对ki(kidney)基因同质型与异质型的母蛾所产未受精卵及受精24h卵的mRNA进行了差异显示研究。通过137组引物组合进行FDD差异显示,结果检出8254条扩增带。其中未受精卵与受精后24h卵之间的差异片段为206条,占总片段数的2.5%,而ki同质型与ki异质型的差异片段为141条,占总片段数的1.7%,其余的95.8%的扩增片段为4方共有,并且约60%的差异带是比较清晰的。回收了较明显的差异片段计199条,占总扩增带数的2.4%,并对其中部分差异片段进行序列分析表明,其中5个克隆序列分别为:smallheatshockproteins、proproteinconvertase、cancerantigenlikeprotein、fusionRNA-bindingprotein及receptorlikeproteinkinase同源序列,其余5个克隆序列未发现同源性,可能是新基因。

关键词：家蚕肾脏形卵荧光差异显示

Multifunctional glycolipids as multi-targeting therapeutics for neural regeneration

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Neural Regeneration Research 2024年第4期19卷 707-708页

作者： yutaka Itokazu Department of Neuroscience and Regenerative Medicine Medical College of GeorgiaAugusta UniversityAugustaGAUSA

Many patients with neurodegenerative diseases,such as Alzheimer’s(AD)and Parkinson’s(PD)diseases suffer from disease progression without any satisfying clinical intervention,likely due to our lack of knowledge on how normal aging impacts the pathogenic mechanisms of these debilitating diseases.A growing body of literature has emerged in recent years that clearly demonstrates the involvement of glycolipids in the protein-oligomerization of neurodegenerative *** hypothesize that changes in glycolipids composition are a common mechanism underlying the shift from healthy brain aging to the neuropathological processes of neurodegenerative diseases.

关键词： lipids involvement diseases

Analysis of deformation mechanisms in magnesium single crystals using a dedicated four-point bending tester

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Journal of Magnesium and Alloys 2024年第5期12卷 1911-1917页

作者： yutaka Yoshida Rikuto Izawa Kenji Ohkubo Faculty of Engineering Kitami Institute of TechnologyKoen-choKitamiHokkaido 090-8507Japan Graduate student Department of Mechanical and Electrical EngineeringKitami Institute of TechnologyKoen-choKitamiHokkaido 090-8507Japan Center for Advanced Research of Materials Faculty of EngineeringHokkaido UniversityKita-kuSapproHokkaido 060-8628Japan Kyokuto Kaihatsu Kogyo Co. Ltd.AwajiChuohuOosakaOosaka 541-0047Japan

In this study,we explored the deformation mechanisms of Mg single crystals using a combination of scanning electron microscopy and electron backscattered diffraction in conjunction with a dedicated four-point bending *** prepared two single-crystal samples,oriented along theanddirections,to assess the mechanisms of deformation when the initial basal slip was *** thesample,the primary{1012}twin(T1)was confirmed along thedirection of the sample on the compression side with an increase in bending *** thesample,T1 and the secondary twin(T2)were confirmed to be along thedirection,with an orientation of±60°with respect to the bending stress direction,and their direction matched with(0001)in T1 and *** result implies that crystallographically,the basal slip occurs *** addition,thesample showed the double twin in T1 on the compression side and the tertiary twin along thedirection on the tension *** results demonstrated that the maximum bending stress and displacement changed significantly under the bend loading because the deformation mechanisms were different for these single ***,the correlation between bending behavior and twin orientation was determined,which would be helpful for optimizing the bending properties of Mg-based materials.

关键词： Four-point bending Magnesium single crystal Twinning Basal slip Scanning electron microscopy Electron backscatter diffraction

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蚕系统发生及进化历史分析

中国科学：生命科学 2012年第6期42卷 489-502,517-518页

作者：孙伟于红松沈以红 banno yutaka 向仲怀张泽西南大学蚕学与系统生物学研究所重庆400716 九州大学遗传资源研究所生物资源及生物环境科学研究院日本福冈812-8581 重庆大学农学及生命科学研究院重庆400044

古丝绸之路是连接古亚洲和古欧洲的纽带.作为重要媒介,家蚕和丝绸在其中发挥着重要作用.家蚕是目前唯一一种被完全驯化的昆虫.然而,利用分子数据研究家蚕起源及进化历史的研究还很少,尤其是家蚕及其祖先野桑蚕的分化时间还有待确定.作... 详细信息

古丝绸之路是连接古亚洲和古欧洲的纽带.作为重要媒介,家蚕和丝绸在其中发挥着重要作用.家蚕是目前唯一一种被完全驯化的昆虫.然而,利用分子数据研究家蚕起源及进化历史的研究还很少,尤其是家蚕及其祖先野桑蚕的分化时间还有待确定.作为重要的驯化物种,家蚕的群体动力学变化历程也鲜有报道.本研究利用线粒体和核基因DNA序列推断了家蚕及其近缘物种的系统发生关系和进化历史.所有系统发生分析结果表明,家蚕和中国野桑蚕具有更近的亲缘关系.家蚕的驯化时间大约为4100年.中国野桑蚕大约在23600年前与日本野桑蚕分开,这个时间与化石证据和历史记载一致.另外,研究结果表明,家蚕在大约1000年前经历了群体扩张.本文对蚕的分化时间及蚕群体动力学进行研究,不仅有利于驯化动物的遗传分析和鳞翅目昆虫的系统发生分析,而且有利于理解人类文明的传播.

关键词：家蚕驯化分化时间群体动力学群体扩张

Next-generation regenerative therapy for ischemic stroke using peripheral blood mononuclear cells

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Neural Regeneration Research 2024年第11期19卷 2341-2342页

作者： Masato Kanazawa Itaru Ninomiya yutaka Otsu Masahiro Hatakeyama Department of Neurology Brain Research InstituteNiigata UniversityNiigataJapan

Stroke is the second leading cause of death and the third leading cause of disability worldwide after heart *** predict that stroke deaths and permanent disabilities will increase worldwide by the year ***-target therapies may be insufficient,because ischemic cerebral injury involves several ***-mediated therapies are ideal,because they target multiple cell types to enhance protection and recovery.

关键词： ischemic death cerebral

Comment on:Recurrence after spontaneous separation of epiretinal membrane in a young woman:a case report

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International Journal of Ophthalmology(English edition) 2024年第4期17卷 783-784页

作者： Yusuke Kameda yutaka Kaneko Karin Ishinabe Nichika Fukuoka Yotsuya-sanchome Ekimae Eye Clinic Tokyo 160-0004Japan Department of Ophthalmology and Visual Sciences Yamagata University Faculty of MedicineYamagata 990-9585Japan

Dear Editor,Herein,we provide a commentary on the recently published article by Zeng QZ and Yu WZ[1].This case report provides interesting novel insights into the recurrence of epiretinal membrane(ERM)following self-separation in a young *** addition to the study,we have been investigating spontaneous ERM release for many years and have recently published a related paper[2].

关键词： separation young release

Study on fibroin heavy chain of the silkworm Bombyx mori by fluorescence in situ hybridization (FISH)

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Science China(Life Sciences) 2002年第6期45卷 663-668页

作者：宋方洲张平波易发平洪锡钧鲁成 yutaka banno Hiroshi Fujii Katsumi Koga College of Basic Medical Sciences Chongqing University of Medical Sciences Chongqing 400016 China Key Sericultural Laboratory of Agriculture Ministry Southwest Agricultural University Chongqing 400716 China Key Sericultural Laboratory of Agriculture Ministry Southwest Agricultural University Chongqing 400716 China School of Life Science Southwest China Normal University Chongqing 400715 China

The sericulture industry plays a very important role in our national economy. Silkworm (Bombyx mori) is always regarded as a model animal and biological reactor. There have been detailed studies on the structure, expression and control and molecular evolution of silk genes. However, few, if any, reports are available on the localization of structural genes in silkworm by molecular cytogenetics. The present experiment has tentatively localized the Fib-H gene at the distal end of the 25th linkage group, namely at the 25-0.0 position, and verified that Fib-H has only one locus, thus providing a temporary solution to the problem about its localization.

关键词： silkworm Bombyx mori, fibroin gene, fluorescence in situ hybridization, gene localization.

Male Morphological Dimorphism in the Genji Firefly Nipponoluciola cruciata in Central Japan

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Advances in Entomology 2024年第3期12卷 203-209页

作者： yutaka Iguchi Laboratory of Biology Okaya Japan

The Japanese Genji firefly Nipponoluciola cruciata is famous for geographic variation in flash pattern. Such geographic variation has also been well studied in terms of ecology and molecular phylogeny. However, there are very few studies on geographic variation in morphology of this species. This study focused on morphological characteristics of males of this species in Yamanashi and Gunma Prefectures separated by the Kanto Mountains in central Japan. Previous studies have shown that this species in the two prefectures exhibits different genetic features as well as different flash patterns. This study aimed to explore morphological characteristics of males of this species and performed multivariate analysis using measurements previously published in the literature. As a result, allometric analysis between body length and elytra width suggested that this species exhibits different allometric patterns between the two prefectures. Moreover, cluster analysis of measurements of genitalia suggested that this species is completely separated into two different groups between the two prefectures. These results suggest the importance of further morphological studies to clarify the divergence of this species.

关键词： Male Dimorphism Allometriy Cluster Gunma Yamanashi