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Self-assessed health and its aspects in the case of Mongolia

Health 2012年第7期4卷 415-422页

作者： Amarsanaa Gan-Yadam Ryoji Shinohara Yuka Sugisawa Emiko Tanaka Taeko Watanabe Maki Hirano Etsuko Tomisaki Kentaro Morita Yoko Onda Yuri Kawashima Kentaro Toketake yukiko Mochizuki Mayumi Nanba Tokie Anme Graduate School of Comprehensive Human Sciences University of Tsukuba Tsukuba Japan

Self health assessment is a simple indicator that is widely used for measuring an individual’s perception of his or her overall health. Many studies have been conducted by way of showing which particular aspects should be included in health self assessment, especially in developed world. In the developing world, however, very few studies have examined self health assessment. The aim of this study was to identify particular aspects and associations in self health assessment and differences between subgroups in Ulaanbaatar, Mongolia. The area sampling approach and Kish tables were used for selecting respondents. Total 500 respondents were surveyed by paper-based questionnaires. Spearman’s analysis and multiple logistic regression analysis were used to show relations between variables. Sociodemographic status, such as age and gender, proved to be important in self health assessment. Other factors affecting self health assessment included the number of family members, daily life and lifestyles, social environment, community motivation, and utilization of health services. Women, the elderly, or people with less-than- normal access to information suffer from poor self-assessed health. People who are socially inactive and who have poor living conditions are less likely to use health care services and are likely to assess their health as “poor.” In order to reduce the poor self-assessed health status it is important to address disparities in socioeconomic factors, such as age, gender, employment, and residential area. In addition, more attention should be paid to community and health services, as well as to health promotion and empowerment activities, including income-generation activities. Further research is also needed, however, for better understanding of about the local people and their health-related issues.

关键词： Self-Assessed Health Socio-Demographic Status Daily Life and Life-Style Community Motivation Social Environment Health Service Utilization and Mongolia

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DRG1促进黑色素瘤细胞增殖的实验研究

DRG1促进黑色素瘤细胞增殖的实验研究

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中国免疫学会第五届全国代表大会暨学术会议

作者：杨小昂彭光勇 yukiko Kiniwa 王荣福郑州大学河南省医学科学研究所美国Baylor医学院细胞和基因治疗中心

目的:研究DRG1(developmentally regulated GTP binding protein 1)在基因敲除前后及过表达的情况下, 对黑色素瘤1558细胞增殖的影响。方法:分别用含有DRG1 siRNA的病毒载体(Lenti-virus vector)、病毒空载体和无关siRNA的病毒载体转导... 详细信息

目的:研究DRG1(developmentally regulated GTP binding protein 1)在基因敲除前后及过表达的情况下, 对黑色素瘤1558细胞增殖的影响。方法:分别用含有DRG1 siRNA的病毒载体(Lenti-virus vector)、病毒空载体和无关siRNA的病毒载体转导黑色素瘤1558细胞,流式细胞计分选含载体的阳性细胞进行实验。用重组DRG1的pcDNA3．1质粒转

关键词： DRG1 siRNA 细胞增殖实验黑色素瘤

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Usefulness of Fractional Exhaled Nitric Oxide-Guided Treatment in Patients with Asthma-Chronic Obstructive Pulmonary Disease Overlap

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Open Journal of Respiratory Diseases 2018年第1期8卷 1-12页

作者： Taisuke Akamatsu Toshihiro Shirai Yuko Tanaka Hirofumi Watanabe Yoshinari Endo yukiko Shimoda Takahito Suzuki Rie Noguchi Mika Saigusa Akito Yamamoto Yuichiro Shishido Takefumi Akita Satoru Morita Kazuhiro Asada Department of Respiratory Medicine Shizuoka General Hospital Shizuoka Japan

Background: Some patients present clinical features of both asthma and chronic obstructive pulmonary disease (COPD), which has led to the recent proposal of asthma-COPD overlap (ACO) as a diagnosis. Fractional exhaled nitric oxide (FeNO) is a candidate biomarker to diagnose ACO. We assessed the effect of an add-on treatment with budesonide/formoterol (BUD/FM) combination in patients with ACO, which was diagnosed by FeNO. Methods: This was a prospective, single-arm, open-label, before and after comparison study. Subjects included 83 patients with COPD who attended outpatient clinics for routine checkups at Shizuoka General Hospital between June and November 2016. All patients fulfilled the GOLD definition of COPD and were receiving long-acting muscarinic antagonist (LAMA) or LAMA/long-acting β2 agonist (LABA) combinations. After an 8-week run-in period, BUD/FM was added to the patients with FeNO levels of ≥35 ppb, defined as having ACO. For patients receiving LAMA/LABA, BUD/FM was added after the discontinuation of LABA. The modified British Medical Research Council (mMRC) score, COPD assessment test (CAT) score, spirometric indices, forced oscillation parameters, and FeNO were assessed before and after 8 weeks of BUD/ FM add-on treatment. Results: Twenty-four patients (28.9%) had FeNO levels ≥ 35 ppb, and 17 patients completed the study (mean age: 73 years and GOLD I/II/III/IV, 5/10/1/1). The mean CAT scores significantly improved (9.2 to 5.4, p = 0.015) and 10 patients (58.8%) showed ≥2 points improvement, a minimal clinically important difference. The mean FeNO levels significantly decreased from 63.0 to 34.3 ppb (p Conclusions: FeNO-guided treatment with BUD/FM improves symptoms in patients with ACO.

关键词： Asthma-COPD Overlap Budesonide/Formoterol Combination COPD Assessment Test Fractional Exhaled Nitric Oxide

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基于增强现实技术的浮迹系统设计

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包装工程 2022年第22期43卷 72-78页

作者：伊丹雪子周星辰饶培伦清华大学北京100084 北京师范大学北京100091

目的基于增强现实技术和弹幕显示方式,设计一套通过文字增强现实环境的系统,帮助用户更有效地获取信息,提升信息交互过程中的趣味性。方法以视频弹幕的特征为基础,针对单用户视频观看场景和多用户社交场景,设计增强现实环境中的文字显... 详细信息

目的基于增强现实技术和弹幕显示方式,设计一套通过文字增强现实环境的系统,帮助用户更有效地获取信息,提升信息交互过程中的趣味性。方法以视频弹幕的特征为基础,针对单用户视频观看场景和多用户社交场景,设计增强现实环境中的文字显示系统——浮迹系统。通过三维动画构建系统功能原型,通过对原型进行用户测试以验证其功能的可用性并找到改进方向。结果在单用户视频观看场景下,用户对于跟踪显示文字满意度最高,浮迹系统能增强用户对观看内容的理解和观看的趣味性。在多用户社交场景下,用户对浮迹系统的满意度受用户本身的社交频率影响很大。结论浮迹系统通过弹幕的形式对现实环境进行增强,为用户提供更多的信息,能有效提升用户对当前环境的理解。在视频观看场景中,该系统能提高观看的趣味性和知识性。在多人社交场景中,该系统能帮助不擅长社交的个体获取有用的社交信息。

关键词：增强现实弹幕文字信息自然交互浮迹系统

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One Step Multiplex PCR for Identification of Candida haemulonii complex and Candia auris

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Advances in Microbiology 2023年第12期13卷 602-613页

作者： Mana Fuchigami Osamu Tsuzukibashi Akira Fukatsu Koji Umezawa Sachiyo Hayashi Yuji Takahashi Hiroshi Yamamoto Chiaki Komine Mio Hagiwara-Hamano yukiko Iizuka Satoshi Uchibori Masanobu Wakami Hiroshi Murakami Taira Kobayashi Masahiko Fukumoto Department of Laboratory Medicine for Dentistry for the Compromised Patient School of Dentistry at Matsudo Nihon University Chiba Japan Department of Special Needs Dentistry School of Dentistry at Matsudo Nihon University Chiba Japan Department of Oral Implantology School of Dentistry at Matsudo Nihon University Chiba Japan Department of Oral Surgery School of Dentistry at Matsudo Nihon University Chiba Japan Department of Fixed Prosthodontics School of Dentistry at Matsudo Nihon University Chiba Japan

Purpose: Recently, Candida haemulonii complex (Candida haemulonii, Candida duobushaemulonii and Candida haemulonii var. vulnera) and two genetically close species (Candida pseudohaemulonii and Candida auris) have emerged as an opportunistic fungal pathogen associated with various infectious diseases of humans, and most of those isolates have displayed antifungal resistance. Because it is difficult to differentiate these microorganisms by a current technique, unfortunately, it is important to establish a method for identifying them accurately. The purpose of the present study was to design species-specific primers in order to identify and detect C. auris, C. pseudohaemulonii, and C. haemulonii complex, i.e. , C. haemulonii, C. duobushaemulonii and C. haemulonii var. vulnera using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the 26S rRNA, 18S rRNA, and RPB1 genes and ITS region of five Candida species. Results: The multiplex PCR method developed in this study was able to distinguish five Candida species clearly. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction.

关键词： Genus Candida Candida haemulonii complex Multiplex PCR Candida auris

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Stratifying the risk of lymph node metastasis in undifferentiated-type early gastric cancer

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World Journal of Gastroenterology 2015年第9期21卷 2683-2692页

作者： yukiko Asakawa Masahiko Ohtaka Shinya Maekawa Mitsuharu Fukasawa Yasuhiro Nakayama Tatsuya Yamaguchi Taisuke Inoue Tomoyoshi Uetake Minoru Sakamoto Tadashi Sato Yoshihiko Kawaguchi Hideki Fujii Kunio Mochizuki Masao Hada Toshio Oyama Tomotaka Yasumura Kosaku Omata Atsushi Nishiyama Keiichi Naito Hideo Hata Yoshiaki Haba Kazuyuki Miyata Haruhisa Saitoh Yoichi Yamadera Kazuo Miura Akira Kawaoi Tohru Abe Hajime Tsunoda Yuji Honda Masayuki Kurosaki Nobuyuki Enomoto First Department of Internal Medicine Faculty of MedicineUniversity of YamanashiYamanashi 409-3898Japan First Department of Surgery Faculty of MedicineUniversity of YamanashiYamanashi 409-3898Japan Department of Pathology Faculty of MedicineUniversity of YamanashiYamanashi 409-3898Japan Department of Surgery Yamanashi Prefectural Central HospitalYamanashi 400-8506Japan Department of Pathology Yamanashi Prefectural Central HospitalYamanashi 400-8506Japan Department of Surgery Social Insurance Yamanashi HospitalYamanashi 400-0025Japan Department of Pathology Social Insurance Yamanashi HospitalYamanashi 400-0025Japan Department of General Surgery Kofu Kyoritsu HospitalYamanashi 400-0034Japan Department of Pathology Kofu Kyoritsu HospitalYamanashi 400-0034Japan Department of Surgery Kofu Municipal HospitalYamanashi 400-0832Japan Department of Pathology Kofu Municipal HospitalYamanashi 400-0832Japan Department of Surgery Yamanashi Kosei HospitalYamanashi 405-0033Japan Department of Surgery Kanoiwa General HospitalYamanashi 405-0018Japan Department of Pathology Kanoiwa General HospitalYamanashi 405-0018Japan Department of Surgery Social Insurance Kajikazawa HospitalYamanashi 400-0601Japan Department of Surgery Kofu National HospitalYamanashi 400-0006Japan Department of Surgery Fujiyoshida Municipal Medical CenterYamanashi 403-0005Japan Division of Gastroenterology and Hepatology Musashino Red Cross HospitalTokyo 180-0023Japan

AIM:To study how lymph node metastasis(LNM) risk is stratified in undifferentiated-type early gastric cancer(undiff-EGC) dependent on combinations of risk ***:Five hundred and sixty-seven cases with undiff-EGC undergoing gastrectomy with lymphadenectomy were examined *** clinicopathological factors of patient age,location,size,an endoscopic macroscopic tumor form,ulceration,depth,histology,lymphatic involvement(LI) and venous involvement(VI),LNM risk was examined and stratified by conventional statistical analysis and datamining ***:LNM was positive in 44 of 567 cases(7.8%).Univariate analysis revealed > 2 cm,protrusion,submucosal(sm),mixed type,LI and VI as significant prognostic factors and > 2 cm and LI-positive were independent factors by multivariate *** preoperatively evaluable factors excluding LVI,sm and > 2 cm were independent *** to the depth and size,cases were categorized into the low-risk group [m and ≤ 2 cm,0%(LNM incidence)],the moderaterisk group(m and > 2 cm,5.6%; and sm and ≤ 2 cm,6.0%),and the high-risk group(sm and > 2 cm,19.3%).On the other hand,LNM occurred in 1.4% in all LI-negative cases,greatly lower than 28.2% in all LI-positive cases,and LNM incidence was low in LInegative cases even in the moderate- and high-risk ***:LNM-related factors in undiff-EGC were depth and size preoperatively while those were LI and size *** these factors,LI was the most significantly correlated factor.

关键词： Undifferentiated-type early gastric cancer Lymph n

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Improvement of prognosis for unresectable biliary tract cancer

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World Journal of Gastroenterology 2013年第1期19卷 72-77页

作者： Takashi Sasaki Hiroyuki Isayama Yousuke Nakai Naminatsu Takahara Naoki Sasahira Hirofumi Kogure Suguru Mizuno Hiroshi Yagioka yukiko Ito Natsuyo Yamamoto Kenji Hirano Nobuo Toda Minoru Tada Masao Omata Kazuhiko Koike Department of Gastroenterology Graduate School of MedicineThe University of TokyoTokyo 113-8655Japan Department of Gastroenterology Kanto Central HospitalTokyo 158-8531Japan Department of Gastroenterology JR Tokyo General HospitalTokyo 151-8528Japan Department of Gastroenterology Japanese Red Cross Medical CenterTokyo 150-8935Japan Department of Gastroenterology Mitsui Memorial HospitalTokyo 101-8643Japan Yamanashi Prefectural Hospital Organization Yamanashi 400-8506Japan

AIM:To evaluate the chemotherapeutic outcomes and confirm the recent improvement of prognosis for unresectable biliary tract ***:A total of 186 consecutive patients with unresectable biliary tract cancer,who had been treated with chemotherapy between 2000 and 2009 at five institutions in Japan,were retrospectively *** patients were divided into three groups based on the year beginning chemotherapy:Group A(2000-2003),Group B(2004-2006),and Group C(2007-2009).The data were fixed at the end of December *** survival and time-to-progression were analyzed and compared ***:No patient characteristics were significantly different among the three *** gallbladder was involved in about half of the patients in each group,and metastatic biliary tract cancer was present in three quarters of the *** Group A,5-fluorouracilbased chemotherapies were primarily selected as firstline chemotherapy,and only 24% were treated with second-line *** Group B,gemcitabine or S-1 monotherapy was mainly introduced as firstline chemotherapy,and 51% of the patients who were refractory to first-line chemotherapy were treated with second-line chemotherapy mainly with *** Group C,the combination therapy with gemcitabine and S-1 was mainly chosen as first-line chemotherapy,and 53% of the patients refractory to first-line chemotherapy were treated with second-line chemotherapy mainly with combination *** median timeto-progressions were 4.4 mo,3.5 mo and 5.9 mo in Groups A,B and C,respectively(4.4 mo vs 3.5 mo vs 5.9 mo,P < 0.01).The median overall survivals were 7.1,7.3,and 11.7 mo in Groups A,B and C(7.1 mo vs 7.3 mo vs 11.7 mo,P = 0.03).Induction rates of all three drugs(gemcitabine,platinum analogs,and fluoropyrimidine) in Groups A,B and C were 4%,2% and 27%(4% vs 2% vs 27%,P < 0.01).CONCLUSION:The prognosis of unresectable biliary tract cancer has improved *** three effective drugs(gemcitabine,p

关键词： Unresectable Biliary tract cancer Gemcitabine Platinum analogs Fluoropyrimidine

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A randomized controlled trial of lifestyle self-monitoring for irritable bowel syndrome in female nursing school students

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Open Journal of Gastroenterology 2013年第8期3卷 328-336页

作者： yukiko Okami Gyozen Nin Kiyomi Harada Masayo Iwasa Kaori Kitaoka Ayako Saruwatari Wataru Aoi Sayori Wada Misaka Kimura Hiroaki Asano Yusuke Okuyama Susumu Takakuwa Motoyori Kanazawa Shin Fukudo Tomiko Tsuji Akane Higashi Department of Behavioral Medicine Tohoku University Graduate School of Medicine Sendai Japan Department of Education Kyoto Women’s University Kyoto Japan Department of Gastroenterology Japan Red Cross Kyoto Daiichi Hospital Kyoto Japan Department of Health and Nutrition Nagoya Bunri University Inazawa Japan Department of Health and Nutrition Nagoya Bunri University Inazawa Japan Ex-Graduate School of Life and Environmental Sciences Kyoto Prefectural University Kyoto Japan Ex-Graduate School of Life and Environmental Sciences Kyoto Prefectural University Kyoto Japan Ex-Graduate School of Life and Environmental Sciences Kyoto Prefectural University Kyoto Japan Department of Health and Nutrition Kyoto Koka Women’s University Kyoto Japan Ex-Graduate School of Life and Environmental Sciences Kyoto Prefectural University Kyoto Japan School of Nursing Kyoto Prefectural University of Medicine Kyoto Japan Faculty of Bioenvironmental Science Kyoto Gakuen University Kameoka Japan Graduate School of Life and Environmental Sciences Kyoto Prefectural University Kyoto Japan School of Nursing Kyoto Prefectural University of Medicine Kyoto Japan

Background: The aim of this study was to verify the efficacy of lifestyle self-monitoring for the improvement of the IBS and reveal what has been changed due to the intervention. Methods: A total of 111 nursing school students were randomized into three groups, two intervention groups (a two-month intervention group, n = 34, and a four-month intervention group, n = 35) and a control group (n = 34). The intervention groups conducted lifestyle self-monitoring in conjunction with a 15-minutes group work for either two or four months. The primary outcome measure was Rome II criteria for IBS. Other outcome measures were the Hospital Anxiety and Depression Scale (HADS) and the Gastrointestinal Symptom Rating Scale (GSRS). They were assessed at the baseline and the end of both of the intervention periods. Analysis was conducted as intention-to-treat. Results: The prevalence of IBS did not change significantly after the intervention in any of the groups. The HAD-A score, a subscale of the HADS score for anxiety, decreased 1.4 points in the two-month intervention group (p = 0.02) and 2.3 points in the four-month intervention group of (p = 0.01) after intervention. The average GSRS decreased 0.2 points in the control group (p = 0.05) and 0.3 points in the four-month intervention group (p < 0.01). Conclusions: Lifestyle self-monitoring for two or four months did not reduce the prevalence of the IBS significantly, but it did decrease anxiety and improved the QOL related to gastrointestinal symptoms in female nursing school students.

关键词： Lifestyle Self-Monitoring Irritable Bowel Syndrome Hospital Anxiety and Depression Scale Gastrointestinal Symptom Rating Scale Nursing School

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Characterization of a novel murine Sost ERT2 Cre model targeting osteocytes

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Bone Research 2019年第1期7卷 41-53页

作者： Delphine B.Maurel Tsutomu Matsumoto Julian A.Vallejo Mark L.Johnson Sarah L.Dallas yukiko Kitase Marco Brotto Michael J.Wacker Marie A.Harris Stephen E.Harris Lynda F.Bonewald Department of Oral and Craniofacial Sciences University of Missouri-Kansas City School of DentistryKansas CityMOUSA Department of Anatomy and Cell Biology Indiana University School of MedicineIndianapolisINUSA Bone.Muscle Collaborative Sciences College of Nursing and Health InnovationUniversity of Texas at ArlingtonArlingtonUSA Department of Biomedical Sciences University of Missouri-Kansas City School of MedicineKansas CityMOUSA University of Texas Health Science Center San AntonioTXUSA Department of Orthopaedic Surgery Indiana University School of MedicineIndianapolisINUSA Pharmaceutical Sciences Department Universite de BordeauxBio-TisINSERM Unité1026 BioTis146 Rue Léo Saignat33076 BordeauxFrance

Transgenic mice are widely used to delete or overexpress genes in a cell specific manner to advance knowledge of bone biology,function and *** numerous Cre models exist to target gene recombination in osteoblasts and osteoclasts,few target osteocytes specifically,particularly mature *** goal was to create a spatial and temporal conditional Cre model using tamoxifen to induce Cre activity in mature osteocytes using a Bac construct containing the 5’and 3’regions of the Sost gene(Sost ERT2 Cre).Four founder lines were crossed with the Ai9 Cre reporter *** founder line showed high and specific activity in mature *** and organs were imaged and fluorescent signal *** no activity was observed in 2 day old pups,by 2 months of age some osteocytes were positive as osteocyte Cre activity became spontaneous or‘leaky’with *** percentage of positive osteocytes increased following tamoxifen injection,especially in males,with 43%to 95%positive cells compared to 19%to 32%in *** signal was observed in any bone surface cell,bone marrow,nor in muscle with or without tamoxifen *** spontaneous signal was observed in any other ***,with tamoxifen injection,a few positive cells were observed in kidney,eye,lung,heart and *** other organs,28 in total,were negative with tamoxifen ***,with age,a muscle phenotype was apparent in the Sost-ERT2 Cre ***,although this mouse model may be useful for targeting gene deletion or expression to mature osteocytes,the muscle phenotype may restrict the use of this model to specific applications and should be considered when interpreting data.

关键词： cell knowledge model

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The efficacy of ma-huang-tang (maoto) against influenza

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Health 2011年第5期3卷 300-303页

作者： Mizue Saita Toshio Naito Soushin Boku yukiko Watanabe Mai Suzuki Fukuko Oka Miki Takahashi Takako Sakurai Eiichiro Sogihara Tomomi Haniu Yuki Uehara Kazunori Mitsuhashi Hiroshi Fukuda Hiroshi Isonuma Keiko Lee Hiroyuki Kobayashi 不详

In this study, we compared Ma Huang Tang (maoto), a traditional Japanese medicine (Kampo), with antiviral drugs to evaluate their respective and combined effect on the duration of fever and other subjective symptoms of influenza. Forty-five patients enrolled in this randomized control trial had positive type A influenza on rapid influenza antigen test, provided written consent, and sought treatment at Juntendo University Hospital between November 2008 and March 2009. Using a computer-gen- erated list, patients were randomly assigned to one of the four intervention groups: 1): maoto (TJ-27), 9 subjects;2): Tamiflu (oseltamivir), 13 subjects;3): Relenza (zanamivir), 6 subjects;and 4): maoto/oseltamivir combination, 9 subjects. Six outcome measures were evaluated, including fever, myalgia, headache, arthralgia, fatigue, and cough. Statistical differences were determined by the Bonferroni-adjusted t-test for multiple comparisons. Our results showed that there were no significant differences among the four groups in the time-course profile of fever and the number of days until fever resolution since treatment was initiated. In addition, no significant intergroup differences were detected in the number of days until resolution of myalgia, headache, fatigue, and cough. However, the maoto group reported a more rapid improvement in joint pain than the oseltamivir group (P = 0.01). In conclusion, maoto showed comparable efficacy as antiviral medications in reducing fever and influenza symptoms. As serious concerns over the indiscriminate use, adverse reactions, and resistance to current antiviral drugs continue to grow, maoto may serve as an elegant option for the treatment of influenza.

关键词： Ma Huang Tang (Maoto) Influenza Joint Pain Oseltamivir and Zanamivir

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