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Bone morphogenetic protein-6 suppresses TGF-β_(2)-induced epithelial-mesenchymal transition in retinal pigment epithelium

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International Journal of Ophthalmology(English edition) 2024年第4期17卷 646-652页

作者： Xuan Liu Ming Liu Meng Ji Bo Ma Yu-Cen Hou Xin-yue yao Qiao-Chu Cheng Li Chen Department of Ophthalmology the First Affiliated Hospital of Xi’an Jiaotong UniversityXi’an 710061Shaanxi ProvinceChina Department of Ophthalmology Xi’an No.1 HospitalXi’an 710000Shaanxi ProvinceChina Shaanxi Institute of Ophthalmology Xi’an 710000Shaanxi ProvinceChina Department of Ophthalmology Ankang Hospital of Traditional Chinese MedicineAnkang 725000Shaanxi ProvinceChina

AIM:To evaluate the effect of bone morphogenetic protein-6(BMP-6)on transforming growth factor(TGF)-β_(2)-induced epithelial-mesenchymal transition(EMT)in retinal pigment epithelium(RPE).METHODS:Adult retinal pigment epithelial cell line(ARPE-19)were randomly divided into control,TGF-β_(2)(5μg/L),and BMP-6 small interfering RNA(siRNA)*** cell morphology was observed by microscopy,and the cell migration ability were detected by Transwell *** EMT-related indexes and BMP-6 protein levels were detected by Western ***,a BMP-6 overexpression plasmid was constructed and RPE cells were divided into the control group,TGF-β_(2)+empty plasmid group,BMP-6 overexpression group,and TGF-β_(2)+BMP-6 overexpression *** EMT-related indexes and extracellular regulated protein kinases(ERK)protein levels were ***:Compared with the control group,the migration of RPE cells in the TGF-β_(2) group was significantly ***-β_(2) increased the protein expression levels ofα-smooth muscle actin(α-SMA),fibronectin and vimentin but significantly decreased the protein levels of E-cadherin and BMP-6(P<0.05)in ***,the migration of RPE cells in the BMP-6 siRNA group was also significantly ***-6 siRNA increased the protein expression levels ofα-SMA,fibronectin and vimentin but significantly decreased the protein expression levels of E-cadherin(P<0.05).Overexpression of BMP-6 inhibited the migration of RPE cells induced by TGF-β_(2) and prevented TGF-β_(2) from affecting EMT-related biomarkers(P<0.05).CONCLUSION:BMP-6 prevents the EMT in RPE cells induced by TGF-β_(2),which may provide a theoretical basis for the prevention and treatment of proliferative vitreoretinopathy.

关键词： bone morphogenetic protein-6 epithelialmesenchymal transition transforming growth factor-β_(2) retinal pigment epithelial cells cell migration

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TL1A Promotes Fibrogenesis in Colonic Fibroblasts via the TGF-β1/Smad3 Signaling Pathway

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Current Medical Science 2024年第3期44卷 519-528页

作者： Jia SONG Dong-lei SUN Chen-yang LI Yu-xin LUO Qian LIU yue yao Hong ZHANG Ting-ting YANG Mei SONG Xin-li BAI Xiao-lan ZHANG Department of Gastroenterology The Second Hospital of Hebei Medical UniversityHebei Key Laboratory of GastroenterologyHebei Institute of GastroenterologyHebei Clinical Research Center for Digestive DiseasesShijiazhuang050000China Department of Pediatrics The Second Hospital of Hebei Medical UniversityShijiazhuang050000China

Objective Intestinal fibrosis is a refractory complication of inflammatory bowel disease(IBD).Tumor necrosis factor ligand-related molecule-1A(TL1A)is important for IBD-related intestinal fibrosis in a dextran sodium sulfate(DSS)-induced experimental colitis *** study aimed to explore the effects of TL1A on human colonic *** A trinitrobenzene sulfonic acid(TNBS)-induced experimental colitis model of LCK-CD2-TL1A-GFP transgenic(Tg)or wild-type(WT)mice was established to determine the effect and mechanism of TL1A on intestinal *** human colonic fibroblast CCD-18Co cell line was treated concurrently with TL1A and human peripheral blood mononuclear cell(PBMC)*** proliferation and activation of CCD-18Co cells were detected by BrdU assays,flow cytometry,immunocytochemistry and Western *** metabolism was tested by Western blotting and real-time quantitative polymerase chain reaction(RT-qPCR).Results The level of collagen metabolism in the TNBS+ethyl alcohol(EtOH)/Tg group was greater than that in the TNBS+EtOH/WT *** growth factor-β1(TGF-β1)and p-Smad3 in the TNBS+EtOH/Tg group were upregulated as compared with those in the TNBS+EtOH/WT *** proliferation of CCD-18Co cells was promoted by the addition of human PBMC supernatant supplemented with 20 ng/mL TL1A,and the addition of human PBMC supernatant and TL1A increased CCD-18Co proliferation by 24.4%at 24 ***1A promoted cell activation and increased the levels of COL1A2,COL3A1,and TIMP-1 in CCD-18Co *** of CCD-18Co cells with TL1A increased the expression of TGF-β1 and *** TL1A promotes TGF-β1-mediated intestinal fibroblast activation,proliferation,and collagen deposition and is likely related to an increase in the TGF-β1/Smad3 signaling pathway.

关键词： tumor necrosis factor ligand-related molecule-1A fibrosis inflammatory bowel disease myofibroblasts transforming growth factor-β1

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NHE7 upregulation potentiates the uptake of small extracellular vesicles by enhancing maturation of macropinosomes in hepatocellular carcinoma

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Cancer Communications 2024年第2期44卷 251-272页

作者： yue yao Yi Xu Liang Yu Ting-Mao Xue Zhi-Jie Xiao Pui-Chi Tin Hiu-Ling Fung Hoi-Tang Ma Jing-Ping Yun Judy Wai Ping Yam Department of Pathology School of Clinical MedicineLi Ka Shing Faculty of MedicineThe University of Hong KongHong KongP.R.China Department of Endocrinology and Metabolism Second Affiliated Hospital of Harbin Medical UniversityHarbinHeilongjingP.R.China Department of Hepatopancreatobiliary Surgery Second Affiliated Hospital of Harbin Medical UniversityHarbinHeilongjingP.R.China State Key Laboratory of Oncology in South China Cancer Center of Sun Yat-sen UniversityGuangzhouGuangdongP.R.China Department of Hepatobiliary Surgery II Zhujiang HospitalSouthern Medical UniversityGuangzhouGuangdongP.R.China Scientific Research Center The Seventh Affiliated HospitalSun Yat-sen UniversityShenzhenGuangdongP.R.China State Key Laboratory of Liver Research The University of Hong KongHong KongP.R.China Department of Pathology Cancer Center of Sun Yat-sen UniversityGuangzhouGuangdongP.R.China

Background:Small extracellular vesicles(sEVs)mediate intercellular commu-nication that contributes to hepatocellular carcinoma(HCC)progression via multifaceted *** success of cell entry determines the effect of sEV on recipient ***,we aimed to delineate the mechanisms underlying the uptake of sEV in ***:AF,Alexa Fluor;ANOVA,analysis of variance;ATP9A,ATPase Phospholipid Transporting 9A;BCECF-AM,2’,7’-bis-(2-barboxyethyl)-5-(and-6)-carboxyfluorescein,acetoxymethyl ester;BSA,bovine serum albumin;CCMR,Centre for Comparative Medicine Research;CRISPR,clustered regularly interspaced short palindromic repeats;CTL,ctrl;CXCR4,C-X-C Chemokine Receptor Type 4;DAPI,4′,6-diamidino-2-phenylindole;DFS,disease-free survival;DMEM,Dulbecco’s Modified Eagle Medium;DMSO,dimethyl sulfoxide;Dox,doxycycline;EEA1,early endosome antigen 1;EIPA,5-(N-ethyl-N-isopropyl)-amiloride;FBS,fetal bovine serum;FITC,fluorescein isothiocyanate;GAPDH,glyceraldehyde-3-phosphate dehydrogenase;GM130,Golgi matrix protein 130;HCC,hepatocellular carcinoma;HEPES,4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid;HPRT1,hypoxanthine phosphoribosyltransferase 1;H-score,histoscore;IAA,indole-3-acetic acid;KD,knockdown;KO,knockout;mAID,mini-auxin-inducible degron;MTT,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide;NHE7,Na(+)/H(+)exchanger 7;ns,non-significant;OD,optical density;OS,overall survival;PBS,phosphate-buffered saline;PCR,polymerase chain reaction;pHe,endosomal pH;pHi,intracellular pH;PKH67,Paul Karl Horan 67;Rab21,Ras-associated binding protein 21;RIPA,radioimmunoprecipitation assay;SAM,synergistic activation mediator;SEMs,standard error of the means;sEVs,small extracellular vesicles;sgRNA,single-guide RNA;shRNA,short-hairpin RNA;SLC9,solute carrier gene 9;SLiCE,Seamless Ligation Cloning Extract;TCGA,The Cancer Genome Atlas;TCL,total cell lysates;TGN,trans-Golgi network;TMA,tissue microarray;TMR,tetramethyl rhodamine;TSG101,tumor susceptibility gene *** yao an

关键词： hepatocellular carcinoma small extracellular vesicles macropinocytosis pH regulation sodium-hydrogen exchanger

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话语延伸及相关现象的研究述评及思考

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语言教学与研究 2024年第3期 79-92页

作者：乔雪玮乐耀厦门大学中文系 361005

口语交际中的话语延伸现象,主要涉及学界以往讨论的倒装、易位、追补、右偏置、句末重复、话尾巴、右缘等相关现象。本文以研究的理论范式和观察视角为线索,梳理和评介了国内外关于话语延伸及相关现象的重要研究成果,探讨了话语延伸现... 详细信息

口语交际中的话语延伸现象,主要涉及学界以往讨论的倒装、易位、追补、右偏置、句末重复、话尾巴、右缘等相关现象。本文以研究的理论范式和观察视角为线索,梳理和评介了国内外关于话语延伸及相关现象的重要研究成果,探讨了话语延伸现象的本质,揭示了以往研究在立足单位和观察视角上的差异,并反思了该领域研究中的一些重要理论问题。

关键词：延伸倒装易位右偏置述评

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汉语会话中协同产出的列举构式及其形式制约

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世界汉语教学 2024年第3期38卷 363-379页

作者：乔雪玮乐耀厦门大学中文系

会话中的列举表达是内外嵌套的互动构式,其外部结构包括引入、列举和完结三个部分,列举本身的内部结构包括列举项和各类列举标记。列举构式在会话中可以由多个交际者协同产出并动态浮现出来。本文发现,会话中协同产出列举构式的列举项... 详细信息

会话中的列举表达是内外嵌套的互动构式,其外部结构包括引入、列举和完结三个部分,列举本身的内部结构包括列举项和各类列举标记。列举构式在会话中可以由多个交际者协同产出并动态浮现出来。本文发现,会话中协同产出列举构式的列举项主要表现为交叠型、嵌入型和延伸型。这些类型的形成既来自于交际者维持会话连续性、建立共鸣和立场表达的功能动因,也受完形心理和临时范畴化的认知因素影响。文章最后探讨了列举构式的核心形式特征在协同产出列举构式中的制约作用。

关键词：列举协同产出临时构式平行性

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Overcoming Spatial Constraints in VR:A Survey of Redirected Walking Techniques

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Journal of Computer Science & Technology 2024年第4期39卷 841-870页

作者： Jia-Hong Liu Yang-Fu Ren Qi Wen Gan Kui Huang Fiona Xiao Yu Chen Er-Xia Luo Khang Yeu Tang yue-yao Fu Cheng-Wei Fan Sen-Zhe Xu Song-Hai Zhang Academy of Arts and Design Tsinghua UniversityBeijing 100084China Department of Computer Science and Technology Tsinghua UniversityBeijing 100084China College of Computer Technology and Application Qinghai UniversityXining 810016China Zhili College Tsinghua UniversityBeijing 100084China School of Computer Beijing Information Science and Technology UniversityBeijing 100192China Department of Physics Tsinghua UniversityBeijing 100084China School of Computer and Communication Engineering University of Science and Technology BeijingBeijing 100083China

As the virtual reality(VR)technology strives to provide immersive and natural user experiences,the challenge of aligning vast virtual environments with limited physical spaces remains *** survey comprehensively explores the advancements in redirected walking(RDW)techniques aimed at overcoming spatial constraints in *** addresses this by subtly manipulating users’physical movements to allow for seamless navigation within constrained *** survey delves into gain perception mechanisms,detailing how slight discrepancies between virtual and real-world movements can be utilized without user awareness,thus extending the effective navigable *** RDW control algorithms for gain-based RDW are analyzed,highlighting their implementation and effectiveness in maintaining immersion and minimizing perceptual ***,novel methods extending beyond traditional gain-based techniques are discussed,showcasing innovative approaches that further refine VR *** practical implications of RDW in enhancing safety and reducing physical collisions in VR environments are underscored,alongside its potential to improve user experience by aligning virtual exploration more closely with natural human behavior *** a thorough review of existing literature and recent advancements,this survey provides a systematic understanding for researchers,developers,and industry *** underscores the importance of RDW in the future of VR,emphasizing RDW's role in making VR more accessible and practical across various applications,from education and training to therapy and *** paper concludes with a forward-looking perspective on the continued evolution and potential of RDW in revolutionizing virtual reality experiences.

关键词： virtual reality(VR) redirected walking(RDW) redirection gain perception

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Impact of fast-track surgery on perioperative care in patients undergoing hepatobiliary surgery

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World Journal of Gastrointestinal Surgery 2024年第10期16卷 3155-3162页

作者： Xiao-Hong Wang Fang-Fang Chen Jia Pan Yun-Fei Jiang Min-yue yao Jia-Li Mao Ya-Feng Xu Department of General Surgery Kecheng District People's HospitalQuzhou 324000Zhejiang ProvinceChina

BACKGROUND Fast-track surgery(FTS)is a modern nursing approach that has gained popularity in the perioperative phase of *** To investigate the impact of FTS on perioperative care for hepatobiliary *** A retrospective analysis was performed on 98 patients who underwent hepato-biliary surgery and were admitted to our hospital from August 2021 to October *** were divided into an observation group and a control group with 49 patients in each group according to different nursing *** control group was treated with standard nursing and the observation group was treated with FTS concept *** length of hospital stay,visual analog scale(VAS)score,wound complications,nursing satisfaction,self-rating scale(SAS)score,and SF-36 quality of life(QoL)score were compared between the two groups before and after *** The duration of hospitalization,hospitalization cost,operation time,first im-plantation time,exhaust time,and first defecation time were shorter than the observation group(P<0.05).Additionally,the observation group showed a sig-nificant difference between the VAS and SAS scores on days 1,3,and 7(P<0.05).The complication rate in the observation group was 4.05%was significantly lower than the 18.36%in the control group,and the comparison groups were statistically significant(χ2=5.018,P=0.025).The observation group had a significantly higher level of nurse satisfaction(94.92%)than the control group(79.59%;χ2=6.078,P=0.014).Both groups showed higher QoL scores after nursing care,with higher scores in the observation group than in the control group(P=0.032).CONCLUSION FTS in patients undergoing hepatobiliary surgery can effectively improve negative mood,QoL,and nursing sa-tisfaction;reduce wound complications;and accelerate patient rehabilitation.

关键词： Rapid rehabilitation surgery concept Hepatobiliary surgery Quality of life Wound complications Fast track surgery

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论维吾尔语bar-“给”义动词的语法化

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民族语文 2024年第1期 76-89页

作者：奥斯曼·阿卜杜克热木乐耀厦门大学中国语言文学系厦门361005

本文通过对古今维吾尔语的考察,分析动词bar-的语法化过程,集中论述利他标记和无阻标记的形成和演变特点。利他意义在历史上通过-(y)U bar-、-I bar-、-A bar-和-0p bar-等不同形式来表达。在语言演变过程中,-pbar-取代其他形式成为现... 详细信息

本文通过对古今维吾尔语的考察,分析动词bar-的语法化过程,集中论述利他标记和无阻标记的形成和演变特点。利他意义在历史上通过-(y)U bar-、-I bar-、-A bar-和-0p bar-等不同形式来表达。在语言演变过程中,-pbar-取代其他形式成为现代维吾尔语表示利他意义的专用标记。此外,利他标记在有些语境中也可用来表示受损义。无阻意义由-A bar-、-0y bar-等形式来表达。在现代维吾尔语中,-0y bar-已消失,-A bar-则经过语音融合而词缀化了。

关键词： bar- 语法化利他标记无阻标记

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坭兴陶在钦州城市公共环境中的应用思路

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丝网印刷 2024年第15期 93-95页

作者：姚樾北部湾大学

现代社会的城市公共环境设计强调与中国优秀传统文化元素的融合,而坭兴陶作为中国四大名陶之一,具有深厚的文化内涵和美学价值。通过分析坭兴陶的艺术特点,调研钦州城市公共环境,结合坭兴陶的艺术特点和钦州城市文化,设计出符合城市特... 详细信息

现代社会的城市公共环境设计强调与中国优秀传统文化元素的融合,而坭兴陶作为中国四大名陶之一,具有深厚的文化内涵和美学价值。通过分析坭兴陶的艺术特点,调研钦州城市公共环境,结合坭兴陶的艺术特点和钦州城市文化,设计出符合城市特色的坭兴陶公共设施,使其成为城市的文化符号和名片。

关键词：坭兴陶景观小品公共环境

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手持式近红外光谱仪测定梨三种品质指标通用模型建模方法研究

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光谱学与光谱分析 2024年第2期44卷 406-412页

作者：毛欣然夏静静徐惟馨韦芸陈玥瑶陈月飞闵顺耕熊艳梅中国农业大学理学院北京100193

梨是生活中非常常见的水果,梨的糖度、酸度(pH)、硬度是评价梨品质的重要指标。近红外光谱技术因快速、无损和高效等优点,广泛应用于检测水果品质指标。手持式近红外光谱仪可以应用于现场无损检测梨品质,梨的大小不同会对梨的光谱和建... 详细信息

梨是生活中非常常见的水果,梨的糖度、酸度(pH)、硬度是评价梨品质的重要指标。近红外光谱技术因快速、无损和高效等优点,广泛应用于检测水果品质指标。手持式近红外光谱仪可以应用于现场无损检测梨品质,梨的大小不同会对梨的光谱和建模产生一定影响。因此,采集大小不同的5个品种梨果(雪梨、红香酥、蜜梨、红肖梨、酸梨)的近红外光谱,最大的雪梨平均赤道周长27.64 cm,重量为362.84 g,最小的蜜梨平均赤道周长18.35 cm,重量为112.67 g,共197个样品。光谱范围为900~1700 cm^(-1),并在梨的赤道上选取三个点测量梨果的可溶性固形物、酸度(pH)与硬度三个化学指标。采集光谱发现,小梨吸光度较大,而大梨吸光度较小。采用三点平均光谱代表样品光谱和一阶导预处理,改善了光谱的一致性,解决了样品不均匀性、不同梨大小不同等因素的影响。线性回归模型PLS可溶性固形物、酸度(pH)和硬度的校正集决定系数依次为0.7394、0.9335、0.8866,预测集决定系数依次为0.7559、0.8734、0.7874,校正集RMSEC依次为0.5504、0.1941、0.5181。预测集RMSEP依次为0.6564、0.2420、0.6692。非线性回归LSSVM模型可溶性固形物、酸度(pH)和硬度的校正集决定系数依次为0.9763、0.9999、0.9960,预测集决定系数依次为0.9234、0.9777、0.9394,校正集RMSEC依次为0.1949、0.0033、0.0894。预测集RMSEP依次为0.3169、0.1089、0.3613。对比线性算法和非线性算法,LS-SVM建模效果明显优于PLS,LS-SVM算法适用于更多的品种、更宽的品质指标范围的样品预测,模型的准确度和稳定性有了显著提高,可以建立不同品种大小的梨的通用模型。手持式近红外光谱仪可用于梨果的糖度、硬度和pH值的快速无损高效检测,并摆脱了实验室的限制,可以实现现场快速检测。

关键词：手持式近红外仪梨偏最小二乘法最小二乘支持向量机

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