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Effects of osmotic stress on antioxidant enzymes activities in leaf discs of P_(SAG12)-IPT modified gerbera

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第7期8卷 458-464页

作者： LAI Qi-xian BAO Zhi-yi ZHU Zhu-jun qian Qiong-qiu MAO Bi-zeng Key Laboratory of Horticultural Plant Development and Biotechnology Department of Horticulture Zhejiang University Hangzhou 310029 China Institute of Biotechnology Zhejiang University Hangzhou 310029 China Department of Horticulture Zhejiang Forestry University Lin＇an 311300 China

Leaf senescence is often caused by water deficit and the chimeric gene PSA612-1PT is an auto-regulated gene delaying leaf senescence. Using in vitro leaf discs culture system, the changes of contents of chlorophylls, carotenoids, soluble protein and thiobarbituric acid reactive substance （TBARS） and antioxidant enzymes activities were investigated during leaf senescence of PSA612-1PT modified gerbera induced by osmotic stress compared with the control plant （wild type）. Leaf discs were incubated in 20%, 40% （w/v） polyethylene glycol （PEG） 6 000 nutrient solution for 20 h under continuous light [ 130 μmol/（m^2·s）]. The results showed that the contents of chlorophylls, carotenoids and soluble protein were decreased by osmotic stress with the decrease being more pronounced at 40% PEG, but that, at the same PEG concentration the decrease in the transgenic plants was significantly lower than that in the control plant. The activities of superoxide dismutase （SOD）, catalases （CAT）, ascorbate peroxidase （APX）, guaiacol peroxidase （GPX） and dehydroascorbate reductase （DHAR） were stimulated by PEG treatment. However, the increases were higher in PSA612-IPT transgenic plants than in the control plants, particularly at 40% PEG treatment. Lipid peroxidation （TBARS content） was increased by PEG treatment with the increase being much lower in transgenic plant than in the control plant. It could be concluded that the increases in the activities ofantioxidant enzymes including SOD, CAT, APX, GPX and DHAR were responsible for the delay of leaf senescence induced by osmotic stress.

关键词： Antioxidant enzymes Gerbera Leaf disc Leaf senescence Osmotic stress PSAG12-IPT

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Paraquat Resistance in Leaf Discs of P_(SAG12)-IPT Modified Gerbera Is Relatedto the Activities of Superoxide Dismutase,Catalase,and Dehydroascorbate Reductase

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Agricultural Sciences in China 2007年第4期6卷 446-451页

作者： LAI Qi-xian BAO Zhi-yi ZHU Zhu-jun MAO Bi-zeng qian Qiong-qiu Department of Horticulture Zhejiang University/Key Laboratory of Horticultural Plant Development and Biotechnology Ministry of Agriculture Hangzhou 310029 P.R.China Institute ofBiotechnology Zhejiang University Hangzhou 310029 P.R.China Zhejiang Forestry University Lin ＇ an 311300 P R. China

In this paper, using in vitro leaf disc culture system, the changes of contents of chlorophylls, carotenoids, soluble protein, thiobarbituric acid reactive substance （TBARS）, and activities of antioxidant enzymes were investigated during the incubation of leaf discs of PSAG12-IPT modified gerbera in 0, 25, 50 μmol L^-1 paraquat （PQ） under continuous light intensity of 130 0tool m-2 s-1, compared with the control plant （wild type）. The results showed that PQ treatment significantly decreased the contents of chlorophylls, carotenoids, and soluble protein, therefore, promoted leaf senescence. However, the decreases in the leaf discs of modified gerbera were considerably smaller. The activities of superoxide dismutase （SOD）, catalase （CAT）, and dehydroascorbate reductase （DHAR） were significantly increased by PQ treatment and with the increasing of PQ concentration, particularly in the modified plants. The activities of ascorbate peroxidase （APX） and guaiacol peroxidase （GPX） could not be detected in the leaf discs of PQ treatments, which suggested that they were labile to the oxidative stress induced by PQ. As a product of lipid peroxidation, TBARS significantly increased in content with the increase of PQ concentration, while its concentration in the modified plants was significantly lower than that of control plants. Therefore, it could be concluded that the chimeric gene PSAG12-IPTtransfonnexi gerbera leaves had higher antioxidative potential, thus causing the delay of senescence under oxidative stress induced by PQ.

关键词： antioxidant enzymes gerbera leaf senescence paraquat oxidative stress PSAG12-IPT

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Oral fibrinogen-depleting agent lumbrokinase for secondary ischemic stroke prevention： results from a multicenter, randomized, parallel- group and controlled clinical trial

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Chinese Medical Journal 2013年第21期126卷 4060-4065页

作者： Cao Yong-jun Zhang Xia Wang Wan-hua Zhai Wan-qing qian Ju-fen Wang Jian-sheng Chen jun You Nian-xing Zhao Zhong Wu qiu-yi Xu Yuan Yuan Lei Li Rui-xia Liu Chun-feng Soochow Univ Affiliated Hosp 2 Dept Neurol Suzhou 215004 Jiangsu Peoples R China Kunshan 1 Peoples Hosp Dept Neurol Kunshan 215300 Jiangsu Peoples R China Taicang 1 Peoples Hosp Dept Neurol Taicang 215400 Jiangsu Peoples R China Zhangjiagang Tradit Chinese Med Hosp Dept Neurol Zhaniagang 215600 Jiangsu Peoples R China Changshu 1 Peoples Hosp Dept Neurol Changshu 215500 Jiangsu Peoples R China Wuxi Tradit Chinese Med Hosp Dept Neurol Wuxi 214000 Jiangsu Peoples R China Changshu 2 Peoples Hosp Dept Neurol Changshu 215500 Jiangsu Peoples R China Suzhou Municipal Hosp Dept Neurol Suzhou 215000 Jiangsu Peoples R China Zhangjiagang 1 Peoples Hosp Dept Neurol Zhangjiagang 215600 Jiangsu Peoples R China Wujiang 1 Peoples Hosp Dept Neurol Wujiang 215200 Jiangsu Peoples R China Taicang Tradit Chinese Med Hosp Dept Neurol Taicang 215400 Jiangsu Peoples R China

Background Elevated fibrinogen （Fg） level is a known risk factor for ischemic stroke. There are few clinical trials on oral fibrinogen-depleting therapies for secondary ischemic stroke prevention. We aimed to assess the effects of one-year therapy with oral lumbrokinase enteric-coated capsules on secondary ischemic stroke prevention.

关键词： ischemic stroke lumbrokinase enteric-coated capsules fibrinogen carotid atherosclerosis

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癌症相关性疼痛病人多维疼痛评估量表的构建及信效度检验

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中国疼痛医学杂志 2024年第3期30卷 196-204页

作者：郑振东黄明金毅罗素霞柳江路平汪子书庄莉余慧青仇晓霞刘波褚倩王昆刘勇任敬王杰军樊碧发中国人民解放军北部战区总医院肿瘤内科沈阳110000 中国人民解放军北部战区总医院疼痛科沈阳110000 中国人民解放军东部战区总医院疼痛科南京210002 河南省肿瘤医院肿瘤内科郑州450003 新疆维吾尔自治区人民医院肿瘤内科乌鲁木齐830001 新乡医学院第一附属医院肿瘤内科新乡453100 蚌埠医科大学第一附属医院肿瘤内科蚌埠233000 云南省肿瘤医院康复与姑息医学科昆明650106 重庆大学附属肿瘤医院老年肿瘤科重庆400030 上海交通大学医学院附属仁济医院肿瘤科及日间化疗中心上海200127 山东省肿瘤医院肿瘤内科济南250117 华中科技大学同济医学院附属同济医院肿瘤科武汉430030 天津市肿瘤医院疼痛科天津300060 徐州市中心医院肿瘤内科徐州221009 中日友好医院疼痛科北京100029

目的:本研究旨在构建适合癌症相关性疼痛(简称癌痛)病人的疼痛评估量表,并探索评估量表用于癌痛病人的信度和效度,以期为临床癌痛病人的管理提供更加综合全面且简便易行的评估工具。方法:基于国内癌痛领域的指导性规范、临床诊疗指南、... 详细信息

目的:本研究旨在构建适合癌症相关性疼痛(简称癌痛)病人的疼痛评估量表,并探索评估量表用于癌痛病人的信度和效度,以期为临床癌痛病人的管理提供更加综合全面且简便易行的评估工具。方法:基于国内癌痛领域的指导性规范、临床诊疗指南、专家共识等,本研究构建了癌痛病人评估量表初始条目池,采取德尔菲法选取我国癌痛领域的二十余名专家进行两轮咨询,统计咨询结果,确认纳入的条目,形成最终的多维疼痛评估量表(multi-dimensional brief test scale for pain,BTS)。经过两轮德尔菲法专家咨询,第一轮纳入21位专家,第二轮纳入10位专家,第一轮纳入19个评估维度,选取重要性评分≥4.5分且变异系数≤20%的7个评估维度,进行第二轮专家咨询。这7个评估维度和条目的重要性结果与第一轮一致,由此最终构建的BTS共包含7个评估维度,15个条目。选取2022年7月至2023年2月中国人民解放军北部战区总医院收治的癌痛病人,使用构建的BTS评估量表进行信效度检验。结果:共纳入166例病人,经过数据清洗,最终纳入分析的病人例数为138例。BTS的信度检测Cronbachα系数为0.78,效度检测的KMO值为0.80,具有较好的信度和效度。结论:构建的BTS条目共纳入7个评估维度,15个条目,具有较好的信度和效度,可在临床实践中推广应用于癌痛病人的疼痛评估。

关键词：癌症相关性疼痛疼痛评估德尔菲法

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Iptakalim ameliorates relaxation to acetylcholine in thoracic aortic rings impaired by microvesicles derived from hypoxia/reoxygenation-treated HUVECs

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中国应用生理学杂志 2016年第6期32卷 481-486页

作者： Kun-wei ZHANG Shao-xun WANG Ye-yi LI Su WEI Man SHANG Chao LIU Miao LIU yi-lu WANG qian ZHU Yan-na WU jun-qiu SONG Yan-xia LIU Department of Pharmacology School of Basic Medical SciencesTianjin Medical University

Objective: To investigate the effect of Iptakalim(Ipt) preventing injury of endothelial microvesicles(EMVs) derived from hypoxia/reoxygenation(H/R)-treated HUVECs on the relaxation of rat thoracic aortic rings and explore the underlying mechanism. Methods: H/R injury model was established to release H/R-EMVs from HUVECs. H/R-EMVs from HUVECs were isolated by ultracentrifugation from the conditioned culture medium. H/R-EMVs were characterized by using Transmission Electron Microscope(TEM). Thoracic aortic rings of rats were incubated with 10^(-7)-10^(-3 )mol/L Ipt and co-cultured with 10 μg/ml H/R-EMVs for 4 hours, and their endothelium- dependent relaxation in response to acetylcholine(ACh) was recorded in vitro. The nitric oxide(NO) production of ACh-treated rat thoracic aortic rings was measured by using Griess reagent. The expression of endothelial NO synthase(e NOS), phosphorylated e NOS(p-e NOS, Ser-1177), serine/threonine kinas(Akt) and phosphorylated Akt(p-Akt, Ser-473) in the thoracic aortic rings of rats was detected by Western blotting. Results: H/R-EMVs were induced by H/R-treated HUVECs and isolated by ultracentrifugation. The isolated H/R-EMVs subjected to TEM revealed small, rounded vesicles(100–1 000 nm) surrounded by a membrane. H/R-EMVs impaired relaxation induced by ACh of rat thoracic aortic rings significantly. Compared with H/R-EMVs treatment individually, relaxation and NO production of rat thoracic aortic rings were increased by Ipt treatment in a concentration-dependent manner(P<0.05, P<0.01). The expression of total e NOS(t-e NOS) and total Akt(t-Akt) was not affected by Ipt or H/R-EMVs. However, the expression of p-e NOS and p-Akt increased after treated with Ipt(P<0.01). Conclusion: Based on H/R-EMVs treatment, ACh induced endothelium-dependent relaxation of rat thoracic aortic rings was ameliorated by Ipt in a concentration-dependent manner. The mechanisms involved the increase in NO production, p-e NOS and p-Akt expression.

关键词：人脐静脉内皮细胞主动脉丝氨酸/苏氨酸激酶复氧缺氧微泡一氧化氮合酶透射电子显微镜

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Preliminary results of environmental monitoring of the natural gas hydrate production test in the South China Sea

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China Geology 2018年第2期1卷 202-209页

作者： Jian-liang Ye Xu-wen Qin Hai-jun qiu qian-yong Liang yi-fei Dong Jian-gong Wei Hai-long Lu Jing-an Lu Yao-hong Shi Chao Zhong Zhen Xia Guangzhou Marine Geological Survey China Geological SurveyGuangzhou 510075China Gas Hydrate Engineering Technology Center China Geologtcal SurveyGuangzhou 510075China College of Engineering Peking UniversityBeijing 100871China

Natural gas hydrate (NGH)is considered as one of the new clean energy sources of the 21st century with the highest *** environmental issues of NGH production have attracted the close attention of scientists in various *** May 10 to July 9,2017,the first offshore NGH production test in the South China Sea (SCS)was conducted by the China Geological *** addition,environmental security has also been effectively guaranteed via a comprehensive environmental monitoring system built during the NGH production *** monitoring system considered sea-surface atmosphere methane and carbon dioxide concentrations,dissolved methane in the sea water column,and the seafloor physical oceanography and marine chemistry *** whole process was monitored via multiple means, in multiple layers,in all domains,and in real *** the production test,an environmental investigation was promptly conducted to evaluate the environmental impact of the NGH production test. The monitoring results showed that the dissolved methane concentration in seawater and the near-seabed environment characteristics after the test were consistent with the background values,indicating that the NGH production test did not cause environmental problems such as methane leakage.

关键词： South China Sea Natural gas hydrate Production test Methane leakage Environmental momtoring

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Evaluation of Renal Impairment in Patients with Diabetic Kidney Disease by Integrated Chinese and Western Medicine

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Chinese Journal of Integrative Medicine 2023年第4期29卷 308-315页

作者： QU yi-lun DONG Zhe-yi CHENG Hai-mei LIU qian WANG qian YANG Hong-tao MAO Yong-hui LI Ji-jun LIU Hong-fang GENG Yan-qiu HUANG Wen LIU Wen-hu XIE Hui-di PENG Fei LI Shuang JIANG Shuang-shuang LI Wei-zhen DUAN Shu-wei FENG Zhe ZHANG Wei-guang LIU Yu-ning TIAN Jin-zhou CHEN Xiang-mei Department of Nephrology First Medical Center of Chinese People's Liberation Army General HospitalNephrology Institute of the Chinese People's Liberation ArmyState Key Laboratory of Kidney DiseasesNational Clinical Research Center for Kidney DiseasesBeijing Key Laboratory of Kidney Disease ResearchBeijing 100853China Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine Key Laboratory of Glucolipid Metabolic DisorderMinistry of Education of ChinaInstitute of Chinese MedicineGuangdong Pharmaceutical UniversityGuangdong Traditional Chinese Medicine Key Laboratory for Metabolic DiseasesGuangzhou 510006China Department of Nephrology The First Affiliated Hospital of Tianjin University of Traditional Chinese MedicineTianjin 300193China Department of Nephrology Beijing HospitalBeijing 100730China Department of Nephrology The Forth Medical Center of People's Liberation Army General HospitalBejing 100039China Department of Endocrinology Dongzhimen Hospital of Beijing University of Traditional Chinese MedicineBejing 100700China Department of Nephrology The Third Medical Center of PLA General HospitalBeijing 100039China Department of Nephrology Beijing Tongren HospitalCapital Medical UniversityBeijing 100730China Department of Nephrology Beijing Friendship HospitalFaculty of Kidney DiseasesCapital Medical UniversityBeijing 100050China Department of Nephrology Dongzhimen Hospital of Beijing University of Traditional Chinese MedicineBeijing 100700China Three Departments of Encephalopathy Dongzhimen Hospital of Beijing University of Chinese MedicineBeijing 100700China

Objective:To investigate the factors related to renal impairment in patients with diabetic kidney disease(DKD)from the perspective of integrated Chinese and Western ***:Totally 492 patients with DKD in 8 Chinese hospitals from October 2017 to July 2019 were *** to Kidney Disease Improving Global Outcomes(KDIGO)staging guidelines,patients were divided into a chronic kidney disease(CKD)1-3 group and a CKD 4-5 *** data were collected,and logistic regression was used to analyze the factors related to different CKD stages in DKD ***:Demographically,male was a factor related to increased CKD staging in patients with DKD(OR=3.100,P=0.002).In clinical characteristics,course of diabetes>60 months(OR=3.562,P=0.010),anemia(OR=4.176,P60 months,anemia,hyperuricemia,massive proteinuria,atherosclerosis,and blood deficiency syndrome might indicate more severe degree of renal function damage in patients with DKD.(Registration ***03865914).

关键词： diabetickidney disease integrated Chinese and Western medicine Chinese medicine syndrome

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丘濬“审几微”思想的道德意蕴与公共治理思维

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南海学刊 2024年第4期10卷 104-112页

作者：高谦中山大学哲学系暨马克思主义哲学与中国现代化研究所广东广州510275

作为儒家道德修养的环节,“诚意”“正心”如何能与“治国”“平天下”相贯通?在《大学衍义补》正文之前,丘濬特意补入一节,指出其中关键在于“审几微”。丘濬的“审几微”思想区分出“几微”的“心”“事”两种类型,提出君子修身的为... 详细信息

作为儒家道德修养的环节,“诚意”“正心”如何能与“治国”“平天下”相贯通?在《大学衍义补》正文之前,丘濬特意补入一节,指出其中关键在于“审几微”。丘濬的“审几微”思想区分出“几微”的“心”“事”两种类型,提出君子修身的为学方法和处理政务的为政方法。其为学方法承续朱熹释“慎独”之意,要求道德主体须时刻审视内心意向,端正行为,具有增强为政者自身修养的道德意蕴。其为政方法则是以“察事几”为关键,从对诸多史事的归纳总结中提升治理主体的历史反思能力,培养其民本意识。并且,要求治理主体具备“谋始”的大局观,抓住事物的开端才能起到防微杜渐和导民向善的作用。从这一道德主体转向治理主体的理路来看,丘濬的“审几微”思想呈现出明显的德治色彩,具有慎初谋始、历史反思与民本内涵,这对于当下提升道德水准和社会治理水平仍有启迪作用。

关键词：丘濬 “审几微” 《大学衍义补》道德意蕴公共治理

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注射用紫杉醇脂质体与紫杉醇注射液在肿瘤患者中的药动学比较

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肿瘤 2011年第12期31卷 1103-1107页

作者：钱隽王漪璇郁韵秋李进复旦大学附属肿瘤医院肿瘤内科复旦大学上海医学院肿瘤学系上海200032 复旦大学药学院上海201203

目的:建立人血浆中紫杉醇(paclitaxel,PTX)浓度的液相色谱-质谱(liquid chromatography-mass spectometry,LC-MS)检测方法,比较注射用紫杉醇脂质体(paclitaxelliposomeforinjection,L-PTX)和常规紫杉醇注射液(conventional paclitaxel injection,C-PTX)在肿瘤患者中的药动学特征。方法:采用随机、开放和对照的试验设计方案。试验分2组,每组各8例患者,分别静脉滴注175mg/m2L-PTX或C-PTX,并于静脉滴注过程中的1.5和3h及滴注结束后的0.25、0.5、1、2、4、8、12、24、36、48和72h采集受试者血样。LC-MS法测定血药浓度,应用DAS2.0软件计算药动学参数并进行比较。结果:患者单次静脉滴注175mg/m2L-PTX与C-PTX的主要药动学参数:血浆峰浓度(Cmax)分别为6455±2247和7400±1542μg/L;药物血浆浓度-时间曲线下面积(area under the plasma concentration-time curve,AUC0-∞)分别为14812±2846和21693±2657μg·h·L-1;血浆消除半衰期(t1/2z)分别为30.5±7.3和13.7±3.2h;表观分布容积(Vz)分别为526.8±112.1和162.9±49.1L/m2;血浆清除率(plasmaclearance,CLz)分别为12.3±2.7和8.2±1.0L·h-1·m-2。经统计学分析,2组的药动学参数差异有统计学意义(P<0.05)。结论:脂质体包裹后改变了PTX的体内药动学特性,与常规PTX相比,脂质体制剂在肿瘤患者体内的分布特性和消除情况有显著不同,具有更好的组织亲和性与缓释作用。

关键词：肿瘤紫杉醇脂质体药代动力学液相色谱-色谱

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Influence of genetic polymorphisms in drug metabolism enzymes and transporters on pharmacokinetics of different fluvastatin formulations

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中国药理学与毒理学杂志 2018年第4期32卷 317-317页

作者： qian XIANG jun-yu XU Ling-yue MA Nan ZHAO Xiao-dan ZHANG qiu-fen XIE Zhuo ZHANG Xia ZHAO yi-min CUI Department of Pharmacy Peking University First Hospital Beijing 100034 China

OBJECTIVE The purpose of the present study was to investigate the impact of *** formulation on the pharmacokinetics-genetic polymorphis *** We compared the difference between the pharmacokinetics of fluvastatin as an extended-release(ER) 80 mg tablet and an immediate-release(IR) 40 mg capsule in terms of drug metabolism enzyme and transporter *** *** this open-label,randomized,two-period,two-treatment,crossover study,*** of BCRP,SLCO1B1,MDR1,CYP2C9,and CYP3A5 polymorphisms on the pharmacokinetics of fluvastatin were analyzed in 24 healthy *** treatment duration was 7 days with a washout period of 7 days between the *** concentration of fluvastatin was evaluated using highperformance liquid chromatography-tandem mass *** The SLCO1 B1 T521 C genotype had no statistically significant effect on IR 40 mg capsule of fluvastatinafter single or repeated ***,for the ER 80 mg tablet,the SLCO1 B1 T521 C genotype correlated with the AUC_(0-24) of repeat doses(P=0.01).The CYP2C9*3 genotype correlated with the AUC_(0-24) after the first dose IR40 mg capsule(P<0.05);however,the difference between CYP2C9*1/*1 and CYP2 C9*1/*3 was not statistically significant after repeated *** The effect of SLCO1B1T521C on *** exposure was observed and was more profound in ER and repeated dose administration than in IR and single dose *** recommend that formulation should be incorporated into future pharmacogenomics studies and clinical implication guidelines.

关键词：氟伐他汀制剂药物动力学遗传学临床分析

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