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Comparative analysis of astaxanthin and its esters in the mutant E1.of Haematococcus pluvialis and other green algae by HPLC with a C30 column

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Science china(Life {1. 2008年第12期51卷 1108-1115页

作者： PENG Juan1, XIANG WenZhou2,1, TANG QuanMing1, SUN Ni1, CHEN feng1,3 & yuan JianPing 4 1 South china Sea institute of oceanology, chinese academy of sciences. Guangzhou 510301, china 2 institute of oceanology, chinese academy of sciences. qingdao 266071, china 3 School of Biological sciences. The University of Hong Kong, Hong Kong, china 4 institute of oceanology & College of Life sciences. Sun Yat-Sen University, Guangzhou 510301, china South china Sea {1. of oceanology Chinese Academy of Sciences Guangzhou 510301 China institute of {1. Chinese Academy of Sciences Qingdao 266071 China School of Biological sciences The University of Hong Kong Hong Kong China institute of {1. & College of Life sciences Sun Yat-Sen University Guangzhou 510301 China

A gradient reversed-phase high-performance liquid chromatography (HPLC) method using a C30 col-umn was developed for the simultaneous determination of astaxanthin, astaxanthin monoesters and astaxanthin diesters in the green algae Chlorococcum sp., Chlorella zofingiensis, Haematococcus plu-vialis and the mutant E1. which was obtained from the mutagenesis of H. pluvialis by exposure to UV-irradiation and ethyl methanesulphonate (EMS) with subsequent screening using nicotine. The re-sults showed that the contents of total astaxanthins including free astaxanthin and astaxanthin esters ranged from 1.4 to 30.9 mg/g dry biomass in these green algae. The lower total astaxanthin levels (2 mg/g dry biomass) were detected in the green algae Chlorococcum sp. and C. zofingiensis. The higher total astaxanthin levels (>1. mg/g dry biomass) were found in the green alga H. pluvialis and its mutant E1. It is notable that the mutant E1.is found to have considerably higher amounts of total astaxanthin (30.9 mg/g) as compared to the wild strain of H. pluvialis (1..1.mg/g). This indicates that UV-irradiation and EMS compound mutagenesis with subsequent screening using nicotine is an effective method for breeding of a high-producing astaxanthin strain of H. pluvialis. In addition, the green alga C. zofingien-sis had a remarkably higher percentage of astaxanthin diesters (76.3% of total astaxanthins) and a re-markably lower percentage of astaxanthin monoesters (1..0% of total astaxanthins) in comparison with H. pluvialis (35.5% for diesters and 60.9% for monoesters), the mutant E1.(49.1. and 48.1.) and Chlorococcum sp. (1..0% and 58.6%).

关键词： green algae Haematococcus pluvialis mutant astaxanthin HPLC C30 column

Further study of sources of the imported cobalt-blue pigment used on Jingdezhen porcelain from late 1. to early 1. centuries

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Science china(Technological {1. 2008年第3期51卷 249-259页

作者： DU feng1 & SU BaoRu2 1.{1. of Chemistry & Molecular Engineering, qingdao University of Science & Technology, qingdao 266000, china 2 College of Information Science Technology, qingdao University of Science & Technology, qingdao 266000, china institute of Chemistry & Molecular Engineering Qingdao University of Science & Technology Qingdao 266000 China College of Information Science Technology Qingdao University of Science & Technology Qingdao 266000 China

Based on the conclusion of modern scientific analysis: the cobalt-blue pigment applied on Jingdezhen official porcelain during yuan Dynasty and prior to Xuande reign of Ming Dynasty (1.71.1.25) contains high iron and low manganese con- tents, and the cobalt during Xuande and after Xuande periods (after 1.26) of Ming Dynasty contains high manganese and low iron contents, in combination with the traditional archaeological method, the related historical textual records were in- vestigated and analyzed, the imported cobalt employed on Jingdezhen official porcelain was studied comprehensively, systematically and logically. In fact, 'Su Ma Li Qing' (Samarra-blue) and 'Su Bo Ni Qing' (Sumatra-blue) are definitely different cobalt ores originated in different places, because of their very similar translitera- tions in {1., the confusion has appeared in ancient books for more than 400 years, which considered 'Su Ma Li Qing' and 'Su Bo Ni Qing' as the same co- balt-blue pigment. In this article the mistake was corrected, the possible producing places and the approximate eras of the two pigments imported to china and used at Jingdezhen were also suggested.

关键词： imported cobalt-blue pigments employed on official blue-and-white porcelain yuan Dynasty early Ming Dy- nasty Mn/Fe content ratio Su Ma Li Qing (Samarra-blue) Su Bo Ni Qing (Sumatra-blue) scientific archae- ological research transliteration

Dissipation source function and an improvement to LAGFD-WAM model

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Acta Oceanologica Sinica 1992年第4期11卷 471-481页

作者： yuan {1., hua feng, Pan Zengdi and Sun Letao 1. First institute of Oceanography, State Oceanic Administration, qingdao 266003, china First institute of Oceanography State Oceanic Administration Qingdao 266003 China China

In this paper, a new theoretical expression of dissipation term is presented on the basis of statistical model of breaking wave, which is an improvement to LAGFD-WAM wave model. The computational results in three typical wind fields show a good improvement to LAGFD-WAM model and a better accuracy in comparison with the observed data in the South china Sea.

关键词： WAM mode Dissipation source function and an improvement to LAGFD-WAM model

Mast cell degranulation-triggered by SARS-CoV-2 induces tracheal-bronchial epithelial inflammation and injury

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Virologica Sinica 2024年第2期39卷 309-318页

作者： Jian-Bo Cao Shu-Tong Zhu Xiao-Shan huang Xing-{1. Wang Meng-Li Wu Xin Li feng-Liang Liu Ling Chen Yong-Tang Zheng Jian-hua Wang Guangzhou institutes of Biomedicine and Health Chinese Academy of SciencesGuangzhou 510530China School of Life sciences.and Medicine University of Science and Technology of ChinaHefei 230026China Key Laboratory of Bioactive Peptides of Yunnan Province Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of SciencesKIZCUHK Joint Laboratory of Bioresources and Molecular Research in Common DiseasesCenter for Biosafety Mega-ScienceKunming Institute of ZoologyChinese Academy of SciencesKunming 650223China University of chinese {1. of sciences Beijing 101408China

SARS-CoV-2 infection-induced hyper-inflammation is a key pathogenic factor of *** research,along with others',has demonstrated that mast cells(MCs)play a vital role in the initiation of hyper-inflammation caused by *** previous study,we observed that SARS-CoV-2 infection induced the accumulation of MCs in the peri-bronchus and bronchioalveolar-duct junction in humanized ***,we found that MC degranulation triggered by the spike protein resulted in inflammation in alveolar epithelial cells and capillary endothelial cells,leading to subsequent lung *** trachea and bronchus are the routes for SARS-CoV-2 transmission after virus inhalation,and inflammation in these regions could promote viral *** are widely distributed throughout the respiratory ***,in this study,we investigated the role of MCs and their degranulation in the development of inflammation in tracheal-bronchial *** analyses showed the accumulation and degranulation of MCs in the peri-trachea of humanized mice infected with *** degranulation caused lesions in trachea,and the formation of papillary hyperplasia was *** transcriptome analysis in bronchial epithelial cells,we found that MC degranulation significantly altered multiple cellular signaling,particularly,leading to upregulated immune responses and *** administration of ebastine or loratadine effectively suppressed the induction of inflammatory factors in bronchial epithelial cells and alleviated tracheal injury in *** together,our findings confirm the essential role of MC degranulation in SARS-CoV-2-induced hyper-inflammation and the subsequent tissue ***,our results support the use of ebastine or loratadine to inhibit SARS-CoV-2-triggered degranulation,thereby preventing tissue damage caused by hyper-inflammation.

关键词： SARS-CoV-2 Mast cell(MC) Bronchial epithelial cell Inflammation Tracheal injury

Bioactive materials from berberine-treated human bone marrow mesenchymal stem cells promote alveolar bone regeneration by regulating macrophage polarization

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Science china(Life {1. 2024年第5期67卷 1010-1026页

作者： Ziyue Qin Yanxing Han Yifei Du Yixuan Zhang Yifeng Bian Ruyu Wang Haoran Wang Fanyi Guo {1. yuan Yongchu Pan Jianliang Jin Qigang Zhou Yuli Wang feng Han Yan Xu Jiandong Jiang Department of Oral and Maxillofacial Surgery The Affiliated Stomatological Hospital of Nanjing Medical UniversityNanjing 210029China Jiangsu Province Key Laboratory of Oral Diseases Nanjing Medical UniversityNanjing 210029China Jiangsu Province Engineering Research Center of Stomatological Translational Medicine Nanjing 210029China State Key Laboratoryof BioactiveSubstances andFunction of Natural Medicine Instituteof Materia MedicaChinese Academyof MedicalSciencesandPeking Union MedicalCollegeBeijing100050China Gusu school Nanjing medical universitySuzhou 215002China Key Laboratory of Cardiovascular&Cerebrovascular Medicine Drug Target and Drug Discovery CenterSchool of PharmacyNanjing Medical UniversityNanjing 211166 China Department of Orthodontics The Affiliated Stomatological Hospital of Nanjing Medical UniversityNanjing 210029China Department of Human Anatomy Research Centre for Bone and Stem CellsKey Laboratory for Aging&DiseaseThe State Key Laboratory of Reproductive MedicineNanjing Medical UniversityNanjing 211166China Department of Clinical Pharmacology School of PharmacyNanjing Medical UniversityNanjing Jiangsu211166China Department of Periodontology The Affiliated Stomatological Hospital of Nanjing Medical UniversityNanjing 210029China Department of Virology Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China

Alveolar bone regeneration has been strongly linked to macrophage polarization.M1.macrophages aggravate alveolar bone loss,whereas M2 macrophages reverse this ***(BBR),a natural alkaloid isolated and refined from chinese medicinal plants,has shown therapeutic effects in treating metabolic *** this study,we first discovered that culture supernatant(CS)collected from BBR-treated human bone marrow mesenchymal stem cells(HBMSCs)ameliorated periodontal alveolar bone *** from the BBR-treated HBMSCs contained bioactive materials that suppressed the M1.polarization and induced the M2 polarization of macrophages in vivo and in *** clarify the underlying mechanism,the bioactive materials were applied to different animal *** discovered macrophage colony-stimulating factor(M-CSF),which regulates macrophage polarization and promotes bone formation,a key macromolecule in the *** of pure M-CSF attenuated experimental periodontal alveolar bone loss in ***-stimulating factor 1.receptor(CSF1.)inhibitor or anti-human M-CSF(M-CSF neutralizing antibody,Nab)abolished the therapeutic effects of the CS of BBR-treated ***,AKT phosphorylation in macrophages was activated by the CS,and the AKT activator reversed the negative effect of the CSF1. inhibitor or *** results suggest that the CS of BBR-treated HBMSCs modulates macrophage polarization via the M-CSF/AKT *** studies also showed that CS of BBR-treated HBMSCs accelerated bone formation and M2 polarization in rat teeth extraction ***,our findings established an essential role of BBR-treated HBMSCs CS and this might be the first report to show that the products of BBR-treated HBMSCs have active effects on alveolar bone regeneration.

关键词： berberine human bone marrow mesenchymal stem cells alveolar bone regeneration macrophage colony-stimulating factor AKT phosphorylation

A salt-induced tackifying polymer for enhancing oil recovery in high salinity reservoirs: Synthesis, evaluation, and mechanism

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GREEN ENERGY & ENVIRONMENT 2024年第11期9卷 1747-1758页

作者： Wu, Yining Li, Peihan Yan, Bin Li, Xiaohan huang, Yongping {1., Juncong feng, Xiang Dai, Caili china Univ Petr East{1. Dept Petr Engn Qingdao 266580 Peoples R China Sichuan Univ Coll Biomass Sci & Engn Dept Natl Engn Lab Clean Technol Leather Manufacture Chengdu 610065 Peoples R China Princeton Univ Dept Civil & Environm Engn Princeton NJ 08544 USA china Univ Petr East{1. Dept Chem Engn Qingdao 266580 Peoples R China

Polymer flooding is an effective method widely applied for enhancing oil recovery (EOR) by reducing the mobility ratio between the injected water and crude oil. However, traditional polymers encounter challenges in high salinity reservoirs due to their salt sensitivity. To overcome this challenge, we synthesized a zwitterion polymer (PAMNS) with salt-induced tackifying property through copolymerization of acrylamide and a zwitterion monomer, methylacrylamide propyl-N, N-dimethylbutylsulfonate (NS). NS monomer is obtained from the reaction between 1.4-butanesultone and dimethylamino propyl methylacrylamide. In this study, the rheological properties, salt responsiveness, and EOR efficiency of PAMNS were evaluated. Results demonstrate that PAMNS exhibits desirable salt-induced tackifying characteristics, with viscosity increasing up to 2.4 times as the NaCl concentration reaches a salinity of 30 x 1.(4) mg L-1. Furthermore, high valence ions possess a much stronger effect on enhancing viscosity, manifested as Mg2+ > Ca2+ > Na+. Molecular dynamics simulations (MD) and fluid dynamics experiment results demonstrate that PAMNS molecules exhibit a more stretched state and enhanced intermolecular associations in high-salinity environments. It is because of the salt-induced tackifying, PAMNS demonstrates superior performance in polymer flooding experiments under salinity ranges from 5 x 1.(4) mg L-1.to 20 x 1.(4) mg L-1. leading to 1..38-1..83% higher EOR than traditional polymers. (c) 2023 institute of Process Engineering, {1. academy of sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://***/licenses/by-nc-nd/4.0/).

关键词： High salinity reservoirs Salt-induced tackifying polymer Anti-polyelectrolyte behavior Enhanced oil recovery ZWITTERIONIC POLYMER BRUSHES SURFACE WATER BEHAVIOR

Mutational Analysis of the Absolutely Conserved B8Gly: Consequence on Foldability and Activity of Insulin

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Acta Biochimica et Biophysica Sinica 2005年第10期37卷 673-679页

作者： Zhan-Yun GUO~1.Zhou ZHANG~1.Xiao-yuan JIA~1.Yue-{1. TANG~(1.2) You-Min feng~(1.2*) 1.Key Laboratory of Proteontics,institute of Biochemistry,and Cell Biology,Shanghai institutes for Biological sciences.chinese academy of sciences.Shanghai 200031.china 2 Instutute of Biochemistry,Zhejiang Sci-Tech University,Hangzhou 31.01.,china KeyLaboratoryofProteomics InstituteofBiochemistryandCellBiologyShanghaiInstitutesforBiologicalSciencesChin

B8Gly is absolutely conserved in insulins during ***,its correspondingposition is always occupied by a Gly residue in other members of insulin *** work showedthat Ala replacement of BSGly significantly decreased both the activity and the foldability of *** effects of substitution are complicated,and different replacements sometimes cause significantly *** analyze the effects of B8 replacement by different amino acids,three new insulin/single-chaininsulin mutants with BSGly replaced by Ser,Thr or Leu were prepared by protein engineering,and both theirfoldability and activity were *** general,replacement of BSGly by other amino acids causes significantdetriment to the foldability of single-chain insulin:the conformations of the three B8 mutants are essentiallydifferent from that of wild-type molecules as revealed by circular dichroism;their disulfide stabilities inredox buffer are significantly decreased:their in vitro refolding efficiencies are decreased approximatelytwo folds;the structural stabilities of the mutants with Ser or Thr substitution are decreased significantly,while Leu substitution has little effect as measured by equilibrium guanidine *** far as biologicalactivity is *** replacement of B8Gly has only a moderate effect:its insulin receptor-bindingactivity is 23% of native *** Thr or Leu replacement produces significant detriment:the receptor-binding potencies of the two mutants are less than 0.2% of native *** present results suggest thatGly is likely the only applicable natural amino acid for the B8 position of insulin where both foldability andactivity are concerned.

关键词： insulin foldability activity disulfide stability

LAGFD-WAM numerical wave model-Ⅰ. Basic physical model

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Acta Oceanologica Sinica 1991年第4期10卷 483-488页

作者： yuan {1., hua feng, Pan Zengdi Sun Letao First institute of Oceanography, State Oceanic Administration, qingdao 266003, china

The LAGFD-WAM wave model is a third generation wave model. In the present paper the physical aspect of the model was shown in great detail including energy spectrum balance equation, complicated characteristics equati... 详细信息

关键词： wave LAGFD-WAM numerical wave model Basic physical model WAM

LAGFD-WAM numerical wave model- Ⅱ. Characteristics inlaid scheme and its application

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Acta Oceanologica Sinica 1992年第1期11卷 13-23页

作者： yuan {1., hua feng, Pan Zengdi Sun Letao First institute of Oceanography, State Oceanic Administration, qingdao 266003, china

In this paper the parameterizational approach of nonlinear source function and the implicit scheme of the model are discussed in detail. The matching problem is solved between time and space steps using the characteristics inlaid scheme with very strong physical meaning. The computational comparison in typical winds shows some improvements to the WAM model. That the hindcast results of the model for typhoon cases are in good agreement with real data illustrates its applicability to wave forecast and engineering study.

关键词： wave mode LAGFD-WAM numerical wave model WAM