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Lactobacillus johnsonii N5 from heat stress-resistant pigs improves gut mucosal immunity and barrier in dextran sodium sulfate-induced colitis

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Animal Nutrition 2023年第4期15卷 210-224页

作者： long yuan Chuyang Zhu Fang Gu Miaonan Zhu Jiacheng Yao Cuipeng Zhu Shicheng Li Kun Wang Ping Hu Yunzeng Zhang Demin Cai Hao-Yu Liu College of Animal Science and Technology Yangzhou UniversityYangzhou 225009China Joint International Research Laboratory of Agricultural&Agri-Product Safety The Ministry of Education of ChinaYangzhou UniversityYangzhou 225009China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou UniversityYangzhou 225009China

Developing effective strategies to prevent diarrhea and associated-gut disorders in mammals has gained great *** to the many health benefits provided by the commensal microbiota of the intestinal tract,such as against environmental perturbation,we explored the host phenotype-associated microbes and their probiotic *** on the observations that the chronic heat stress-exposed weaned piglets present as heat stress-susceptible(HS-SUS)or heat stress-resistant(HS-RES)individuals,we confirmed the phenotypic difference between the two on growth performance(P<0.05),diarrhea index(P<0.001),intestinal heat shock protein 70(HSP70)regulation(P<0.01),and inflammatory responses(P<0.01).By comparing the gut microbiome using 16S rRNA gene sequencing and KEGG functional analysis,we found that Lactobacillus johnsonii exhibited significantly higher relative abundance in the HS-RES piglets than in the HS-SUS ones(P<0.05).Further experiments using a mouse model for chemical-induced inflammation and intestinal injury demonstrated that oral administration of a representative *** N5(isolated from the HS-RES piglets)ameliorated the clinical and histological signs of colitis while suppressing intestinal pro-inflammatory cytokines TNF-a and IL-6 production(P<0.05).We found that N5 treatment enhanced tight junction proteins ZO-1 and occludin and cytoprotective HSP70 levels under physiological condition and restored their mucosal expressions in colitis(P<0.05).In support of the high production of the anti-inflammatory cytokine IL-10,N5 promoted the intestinal Peyer’s patches MHCⅡ+and CD103+dendritic cell populations(P<0.05),increased the regulatory T(Treg)cell numbers(P<0.05),and decreased the Th17 population and its IL-17a production under physiological condition and during colitis(P<0.01).Our results shed light on understanding the interaction between commensal Lactobacillus and the host health,and provide *** N5 as an alternative to antibiotics for p

关键词： Lactobacillus johnsonii Probiotic Heat shock protein Diarrhea Heat stress Treg/Th17

Effect of hot-band annealing process on microstructure,texture,and r-value of final sheets of ferritic stainless steel

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Baosteel Technical Research 2019年第4期13卷 1-7页

作者： YU Haifeng DU Wei yuan long Research Institute Baoshan Iron&Steel Co.Ltd.Shanghai 201999China Baoxin Stainless Steel Co. Ltd.Ningbo 315807ZhejiangChina

In this paper,the effect of different annealing processes on the microstructure,texture,and formability of ferritic stainless steel was studied in *** results showed that the grain size in the final sheet was larger and the recrystallization texture was more uniform after the final recrystallization annealing of hot-rolled steel with continuous annealing than that without annealing or with batch *** addition,the final sheet had a higher average r-value and the lowest planar anisotropy.

关键词： hot-band annealing process microstructure texture formability ferritic stainless steel

Investigation of PAT for Advanced Sulphoaluminate MDF Cement

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Journal of Wuhan University of Technology(Materials Science) 2005年第1期20卷 104-105页

作者：黄从运 School of Materials Science and Engineering Wuhan University o f Technoloy Wuhan 430070China

The method of examining micro-pores in su lp hoaluminate MDF cement by means of the Positron Annihilation Technique (PAT) was *** results show that the intensity I 2 of positrons annihilation wi th moderate life-span (τ 2≈330Ps) increases regularly with the addition of t he numbers of micro-pores (less than 250?). Therefore, the value of I 2 may b e used to opitomize the compactness and integrity of the MDF cement structure. A t the same time, the experiment result of PAT was compared with that of Mercury Intrusion Porosimeter (MIP).

关键词： sulphoaluminate MDF cement PAT MIP polyvinyl alcohol

PREPARATION AND EVALUATION OF HPLC BONDED PHASE WITH L-PROLINE LIGAND

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Chinese Chemical Letters 1992年第3期3卷 197-200页

作者： Tian Bao HUANG Bang Gui WU yuan De long Jing Xian ZHOU Chengdu Institute of Organic Chemistry Academia SinicaChengdu 610015

This paper describes a simple method for the preparation of L-proline stationary phase bonded to silica gel and characterization of the bonded phase by IR spectrometry, elemental analysis and nitrogen adsorption method at low *** enantiomeric resolutions of 3-(2-pyridyl)-3-aminopropionic acid and 2,3-diaminobutanoic acid on the bonded phase were carried out.

关键词： Pro CSP PREPARATION AND EVALUATION OF HPLC BONDED PHASE WITH L-PROLINE LIGAND HPLC

Preparation of Electrospun CdSe-Quantum-Dots-Doped Porous Films

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Journal of Donghua University(English Edition) 2014年第5期31卷 609-616页

作者： GAO Bing SHEN Chao yuan Shuang-long ZHANG Jian-guang YANG Yun-xia MO Xiu-mei LI Hai-yan CHEN Guo-rong Key Laboratory for Ultrafine Materials of Ministry of Education School of Materials Science and Engineering East ChinaUniversity of Science and Technology Shanghai 20023 7 China College of Chemistry Chemical Engineering and Biotechnology Donghua University Shanghai 201620 China Med-X Research Institute School of Biomedical Engineering Shanghai Jiaotong University Shanghai 200030 China

Electrospun porous films doped with the green-synthesized CdSe quantum dots were synthesized. Glycerol was chosen to prepare the quantum dots （ QDs）, with the highest quantum yield of 78.28%. Polycaprolactone （PCL） was electrospun with CdSe QDs to avoid the QDs＇ toxicity and improve the QDs＇ cytocompatibility. The electrospun QDs-doped films preserve the original QDs＇ fluorescence. Pores can be detected from the SEM of the films, predicting the possibility of loading drugs in the cancer therapy. The cell proliferation assay shows excellent cytocompatibility of the eletrospun CdSe-QDs-doped films. The present eletrospun CdSe- QDs-doped porous films are cytocompatibale, highly-fluorescent and ootential to load drugs in cancer therapy.

关键词： CdSe-quantum dots (QDs ) electrospun films polycaprolactone (PCL) photoluminescence (PL) cytocompatibility cell proliferation assay

Microstructural characteristics and mechanical properties of bronze/steel bimetallic laminated composite prepared by protective atmosphere casting process

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China Foundry 2021年第5期18卷 497-504页

作者： Bin Liu Da-shan Sui Zhi-min Guo yuan-long Wang Xiao-lin Yang Guang-hong Hu Ji-xian Zhang Chang-zeng Luo Research Institute of Hydraulic Transmission Linde Hydraulics(China)Co.Ltd.Weifang 261061ShandongChina National Engineering Research Center of Die&Mold CAD Shanghai Jiao Tong UniversityShanghai 200030China

The bonding quality of bronze/steel bimetallic laminated composite is the key factor to whether it can be used in poor working *** this study,bimetallic billets were prepared by the protective atmosphere casting *** microstructural characteristics,mechanical properties and interface bonding mechanism of the bronze/steel bimetallic composites were analyzed comprehensively through a series of microstructure analysis(inicluding OM,SEM,and EDS)and mechanical properties *** experimental results demonstrate that the vaporization of lead(Pb)element in the bronze is only 0.4%in weight,and alloy elements are diffused into both sides at the ***,the hardness in the bronze region exceeds 100.0 HV which indicates excellent wear *** and shear tests indicate that the fractures mainly occur on the bronze side with the interaction of the brittle fracture of Pb particles and the ductile fracture of Cu-Sn solid *** maximum tensile strength and shear strength reach 204.0 MPa and 211.0 MPa,*** results demonstrate that the protective atmosphere casting process is applicable for the preparation of bronze/steel bimetallic laminated composite with high quality.

关键词： protective atmosphere casting process lead bronze bimetallic composite microstructure mechanical properties

The reaction of perfluoroalkanesulfinates——Ⅶ.Fenton reagent-initiated addition of sodium perfluoroalkanesulfinates to alkenes

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Chinese Journal of Chemistry 1992年第4期10卷 365-372页

作者： HUANG,Wei-yuan L,long Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences,Shanghai 200032

The addition of sodium perfluoroalkanesulfinates (R_FSO_2Na) to various olefins (CH_2=CHR) initiated by Fenton reagent (Fe^(2+)-H_2O_2) in the presence of sodium azide gave the adduets R_FCH_2- CHN_3R in good yield.A radical mechanism was proposed based on the EPR and other evidences. The adducts were readily reduced through catalytic hydrogenation to give the corresponding amines R_FCH_2CH(NH_2)R in high *** reaction represents a convenient and effective route to these useful organofluorine compounds.

关键词： The reaction of perfluoroalkanesulfinates Fenton reagent-initiated addition of sodium perfluoroalkanesulfinates to alkenes

异质同形:大陆与香港及东南亚华文微型小说之比较——以《微型小说选刊》、《小小说选刊》与香港获益出版微型小说集为视角

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中国文学研究 2017年第1期 121-126页

作者：袁龙湖南师范大学文学院湖南长沙410081 邵阳学院中文系湖南邵阳422000

本文以大陆《微型小说选刊》、《小小说选刊》和香港获益出版公司刊行的微型小说集为样本,梳理1985年以来中国大陆与香港及东南亚华文微型小说发展路径,二者在内容与形式、技巧上具有"同中有异,异中有同"的特点。香港及东南亚华文微型... 详细信息

本文以大陆《微型小说选刊》、《小小说选刊》和香港获益出版公司刊行的微型小说集为样本,梳理1985年以来中国大陆与香港及东南亚华文微型小说发展路径,二者在内容与形式、技巧上具有"同中有异,异中有同"的特点。香港及东南亚华文微型小说在与异质文化碰撞融合之后仍能彰显中华文化和传统美德,葆有走向世界所应有的民族底色,其文学体式与审美意蕴对于当代中国文学创作与研究具有一定参考价值。

关键词：微型小说《小小说选刊》《微型小说选刊》香港获益出版中华文化异质同形

Detection of Tocopherol in Oilseed Rape (Brassica napus L.) Using Gas Chromatography with Flame Ionization Detector

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Journal of Integrative Agriculture 2013年第5期12卷 803-814页

作者： Nazim Hussain Zahra Jabeen LI yuan-long CHEN Ming-xun LI Zhi-lan GUO Wan-li Imran Haider Shamsi CHEN Xiao-yang JIANG Li-xi Key Laboratory of Crop Germplasm Resources of Zhejiang Province College of Agriculture and BiotechnologyZhejiang University

The variation among Chinese genotypes of Brassica napus L. for seed tocopherols content and their analysis using gas chromatography has not been comprehensively reported till to date. In the present study, the tocopherol contents of four Chinese genotypes of Brassica napus L., namely, Gaoyou 605, Zhejiang 619, Zheshuang 758, and Zheshuang 72, were evaluated using three modified sample preparation protocols （P1, P2, and P3） for tocopherol extraction. These methods were distinguished as follows. Protocol one （P1） included the evaporation of solvent after extraction without silylation. Protocol two （P2） followed the direct supernatant collection after overnight extraction without drying and silylation. Protocol three （P3） included trimethylsilylation with N,O-bis（trimethylsilyl） trifluoroacetamide. Genotypic comparison of tocopherol and its isoforms revealed that Gaoyou 605 was dominant over the other genotypes with （140.5＋ 10.5）, （316.2＋ 9.2）, and （559.1＋ 24.3） ~tg g-~ of seed meal ct-, 7-, and total （T-） tocopherol, respectively, and a 0.44＋0.04 ^- to 7-tocopherol ratio. The comparison of the sample preparation protocols, on the other hand, suggests that P3 is the most suitable method for the tocopherol extraction from Brassica oilseeds and for the analysis of tocopherols using gas chromatography flame ionization detector （GC-FID）. Trimethylsilylation is the key step differentiating P3 from P1 and P2. Variations detected in tocopherol contents among the Chinese rapeseed （B. napus） genotypes signify the need to quantify a wide range of rapeseed germplasm for seed tocopherol dynamics in short and crop improvement in long.

关键词： Brassica napus L. genotypic variation tocopherol analysis gas chromatography flame ionization detector