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Transcriptomic analysis reveals the transcription factors involved in regulating the expression of EPSPS gene,which confers glyphosate resistance of goosegrass (Eleusine indica)

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Journal of Integrative Agriculture 2021年第8期20卷 2180-2194页

作者： ZHANG Chun yu chao-jie ZHANG Tai-jie GUO Wen-lei TIAN Xing-shan Guangdong Provincial Key Laboratory of High Technology for Plant Protection Institute of Plant ProtectionGuangdong Academy of Agricultural SciencesGuangzhou 510640P.R.China

Glyphosate inhibits the enzyme 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS)and overexpression of the EPSPS gene is one of the molecular mechanisms conferring glyphosate resistance in weeds.A regulatory sequence of EPSPS gene was isolated previously,and an alteration in its 5´-untranslated region(UTR)pyrimidine(Py)-rich stretch element is involved in the regulation of EPSPS expression in glyphosate-resistant(GR)Eleusine ***,the transcription factors involved in this regulatory sequence remain to be *** this study,we investigated the regulatory network of EPSPS overexpression associated genes in a GR *** population by *** differentially expressed transcript analyses revealed that glyphosate treatment caused an increase in the expression of 2752 unigenes and a decrease in the expression of 4025 unigenes in the GR ***,compared to the glyphosatesusceptible(GS)*** them,1373 unigenes were identified to be co-expressed with the EPSPS gene in GR *** and KEGG pathway analyses showed that the up-regulated unigenes were mainly enriched in chloroplasts and associated with the shikimate biosynthesis pathway,chlorophy II and peroxisome metabolism ***,the expression of a Shikimate kinase which catalyzed the conversion of Shikimate to Shikimate 3-phosphate(S3P,a substrate of EPSPS),was also *** transcription factors were identified as likely to be involved in the regulation of the EPSPS expression,and three of them(ARF2,ARF8 and BPC6)showed more binding sites because of a(CT)n insertion of the 5´-UTR Py-rich stretch element in ***,the yeast one-hybrid assay illustrated that ARF8 and BPC6 could bind to the 5´-UTR Py-rich stretch element of wild type EPSPS,but could not bind to the mutated *** data suggests that the transcriptional regulation of EPSPS expression is complex and was significantly altered in GR *** discoveries provide new references for further study of the

关键词： transcriptomic EPSPS 5´-UTR Py-rich stretch element transcription factor glyphosate resistance

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Resistance of Cement-based Grouting Materials with Nano- SiO_(2) Emulsion to Chloride Ion Penetration

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 114-119页

作者： LI Shuiping CHENG Jian WEI chao yuAN Bin yu Chengxiao College of Architectural Science and Engineering Yangzhou UniversityYangzhou 225127China

The chloride penetration resistance of cement-based grout materials was improved by nano-silica *** of mixtures containing different nano-silica particles or emulsions were exposed in sodium chloride solutions of specific concentrations with different test *** properties of the mixes were assessed in terms of weight loss and compressive strength.X-ray diffraction(XRD)and scanning electron microscopy(SEM)of mixes were performed to analysis the phase evolution and *** results demonstrated that the introduction of nano-SiO_(2) emulsion significantly decreased the compressive strength loss and calcium hydroxide(CH)crystal content of hydration production,and then enhanced the resistance of cement-based grouting materials to chloride ion *** improvement derives from the filling and pozzolanic effects of nano-SiO_(2) particles,which were incorporated via an emulsion and attributed to a well dispersion in grouting matrix.

关键词： grouting materials nano-SiO_(2)emulsion chloride ion penetration,weight loss strength loss

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Ideal Bi‑Based Hybrid Anode Material for Ultrafast Charging of Sodium‑Ion Batteries at Extremely Low Temperatures

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Nano-Micro Letters 2025年第3期17卷 152-167页

作者： jie Bai Jian Hui Jia yu Wang Chun Cheng Yang Qing Jiang Key Laboratory of Automobile Materials(Jilin University) Ministry of EducationSchool of Materials Science and EngineeringJilin UniversityChangchun 130022People’s Republic of China

Sodium-ion batteries have emerged as competitive substitutes for low-temperature applications due to severe capacity loss and safety concerns of lithium-ion batteries at−20°C or ***,the key capability of ultrafast charging at ultralow temperature for SIBs is rarely ***,a hybrid of Bi nanoparticles embedded in carbon nanorods is demonstrated as an ideal material to address this issue,which is synthesized via a high temperature shock *** a hybrid shows an unprecedented rate performance(237.9 mAh g^(−1)at 2 A g^(−1))at−60℃,outperforming all reported SIB anode *** with a Na_(3)V_(2)(PO_(4))_(3)cathode,the energy density of the full cell can reach to 181.9 Wh kg^(−1)at−40°*** on this work,a novel strategy of high-rate activation is proposed to enhance performances of Bi-based materials in cryogenic conditions by creating new active sites for interfacial reaction under large current.

关键词： Bi nanoparticles High temperature shock High-rate activation Ultrafast charging Low-temperature sodium-ion batteries

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Flexible Graphene Field‑Effect Transistors and Their Application in Flexible Biomedical Sensing

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Nano-Micro Letters 2025年第2期17卷 252-313页

作者： Mingyuan Sun Shuai Wang Yanbo Liang chao Wang yunhong Zhang Hong Liu yu Zhang Lin Han Institute of Marine Science and Technology Shandong UniversityQingdao 266237ShandongPeople’s Republic of China State Key Laboratory of Crystal Materials Shandong UniversityJinan 250100ShandongPeople’s Republic of China School of Integrated Circuits Shandong UniversityJinan 250100ShandongPeople’s Republic of China Shandong Engineering Research Center of Biomarker and Artificial Intelligence Application Jinan250100ShandongPeople’s Republic of China

Flexible electronics are transforming our lives by making daily activities more *** to this innovation are field-effect transistors(FETs),valued for their efficient signal processing,nanoscale fabrication,low-power consumption,fast response times,and ***,known for its exceptional mechanical properties,high electron mobility,and biocompatibility,is an ideal material for FET channels and *** combination of graphene and FETs has given rise to flexible graphene field-effect transistors(FGFETs),driving significant advances in flexible electronics and sparked a strong interest in flexible biomedical ***,we first provide a brief overview of the basic structure,operating mechanism,and evaluation parameters of FGFETs,and delve into their material selection and patterning *** ability of FGFETs to sense strains and biomolecular charges opens up diverse application *** specifically analyze the latest strategies for integrating FGFETs into wearable and implantable flexible biomedical sensors,focusing on the key aspects of constructing high-quality flexible biomedical ***,we discuss the current challenges and prospects of FGFETs and their applications in biomedical *** review will provide valuable insights and inspiration for ongoing research to improve the quality of FGFETs and broaden their application prospects in flexible biomedical sensing.

关键词： Flexible Graphene Field-effect transistor Wearable Implantable Biosensor

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Mechanistic insight into the synergy between nickel single atoms and nanoparticles on N-doped carbon for electroreduction of CO2

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Journal of Energy Chemistry 2025年第1期100卷 327-336页

作者： Mingdong Sun Wenwen Guan Cailing Chen chao Wu Xiaoling Liu Biao Meng Tao Chen yu Han Jun Wang Shibo Xi yu Zhou State Key Laboratory of Materials-Oriented Chemical Engineering College of Chemical Engineering Nanjing Tech University Advanced Membranes and Porous Materials Center Physical Sciences and Engineering Division King Abdullah University of Science and Technology Institute of Sustainability for Chemicals Energy and Environment A*STAR (Agency for Science Technology and Research) College of Materials Science and Engineering Sichuan University

The synergy of single atoms （SAs） and nanoparticles （NPs） has demonstrated great potential in promoting the electrocatalytic carbon dioxide reduction reaction （CO2RR）;however,the rationalization of the SAs/NPs proportion remains one challenge for the catalyst ***,a Ni2+-loaded porous poly（ionic liquids）(PIL) precursor synthesized through the free radical self-polymerization of the ionic liquid monomer,1-allyl-3-vinylimidazolium chloride,was pyrolyzed to prepare the Ni,N co-doped carbon materials,in which the proportion of Ni SAs and NPs could be facilely modulated by controlling the annealing *** catalyst Ni-NC-1000 with a moderate proportion of Ni SAs and NPs exhibited high efficiency in the electrocatalytic conversion of CO2into *** Ni K-edge X-ray absorption near-edge structure （XANES） spectra and theoretical calculations were conducted to gain insight into the synergy of Ni SAs and *** charge transfer from Ni NPs to the surrounding carbon layer and then to the Ni SAs resulted in the electron-enriched Ni SAs active *** the electroreduction of CO2,the coexistence of Ni SAs and NPs strengthened the CO2activation and the affinity towards the key intermediate of*COOH,lowering the free energy for the potential-determining*CO2→*COOH step,and therefore promoted the catalysis efficiency.

关键词： Activation energy

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基于ACO_PSO的机器人路径规划和ROBCAD运动仿真

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组合机床与自动化加工技术 2017年第5期 111-113,125页

作者：冯超钰王杰张梦超四川大学制造科学与工程学院成都610065

汽车焊装是汽车生产中的重要环节,目前生产人员主要依靠经验进行焊点路径规划,导致工作量大、效率低。针对这一问题,提出了用ACO_PSO混合算法计算得出合理的焊接路径,并与实际工人规划的传统路径进行比较,再与ACO算法和PSO算法的实验结... 详细信息

汽车焊装是汽车生产中的重要环节,目前生产人员主要依靠经验进行焊点路径规划,导致工作量大、效率低。针对这一问题,提出了用ACO_PSO混合算法计算得出合理的焊接路径,并与实际工人规划的传统路径进行比较,再与ACO算法和PSO算法的实验结果比较,从而证明该算法更优。然后在ROBCAD中建立三维仿真模型,解决了可达性、干涉等实际生产过程中的常见问题,最后计算出焊接过程所需的时间。研究结果表明,ACO_PSO混合算法对改善机器人节拍和提高生产效率有一定的帮助,进一步提出了焊接路径规划的研究重点和发展方向。

关键词： ACO_PSO 焊接路径 ROBCAD 仿真

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煤矿机电设备安装要点及安装质量控制探讨

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电力系统装备 2020年第1期 53-54页

作者：于超杰陕西彬长大佛寺矿业有限公司陕西咸阳712000

这些年来,工业进程不断加快,煤矿企业的发展趋势也越来越好,所以在如今的煤矿工程中,人们的要求也提高了。为了提高工作效率,也为了工作更加简单方便,人们开始将煤矿工作和机电设备结合起来,提高了煤矿行业的发展效率。煤矿机电安装中... 详细信息

这些年来,工业进程不断加快,煤矿企业的发展趋势也越来越好,所以在如今的煤矿工程中,人们的要求也提高了。为了提高工作效率,也为了工作更加简单方便,人们开始将煤矿工作和机电设备结合起来,提高了煤矿行业的发展效率。煤矿机电安装中要把工作落到实处,要实施系统化的方案,必须做好监督管理。细节化管理在安装过程中必不可少,确保每个环节的万无一失,才能大大提升工作质量,提高设备精度。基于这些情况,文章分析了与安装机电设备有关的因素,也为企业质控提出了建议。

关键词：煤矿行业机电联合设备安装工程质量提高

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CNKSR2 interactome analysis indicates its association with the centrosome/microtubule system

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Neural Regeneration Research 2025年第8期20卷 2420-2432页

作者： Lin Yin Yalan Xu jie Mu yu Leng Lei Ma yu Zheng Ruizhi Li Yin Wang Peifeng Li Hai Zhu Dong Wang Jing Li Institute for Translational Medicine The Affiliated Hospital of Qingdao UniversityMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina School of Basic Medicine Qingdao UniversityQingdaoShandong ProvinceChina College of Life Sciences and School of PharmacyMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina Department of Urology Qingdao Municipal Hospital Affiliated to Qingdao UniversityQingdaoShandong ProvinceChina

The protein connector enhancer of kinase suppressor of Ras 2(CNKSR2),present in both the postsynaptic density and cytoplasm of neurons,is a scaffolding protein with several protein-binding *** of the CNKSR2 gene have been implicated in neurodevelopmental disorders,particularly intellectual disability,although the precise mechanism involved has not yet been fully *** has demonstrated that CNKSR2 plays a role in facilitating the localization of postsynaptic density protein complexes to the membrane,thereby influencing synaptic signaling and the morphogenesis of dendritic ***,the function of CNKSR2 in the cytoplasm remains to be *** this study,we used immunoprecipitation and high-resolution liquid chromatography-mass spectrometry to identify the interactors of *** a combination of bioinformatic analysis and cytological experiments,we found that the CNKSR2 interactors were significantly enriched in the proteome of the *** also showed that CNKSR2 interacted with the microtubule protein DYNC1H1 and with the centrosome marker *** colocalization analysis confirmed the centrosomal localization of *** we downregulated CNKSR2 expression in mouse neuroblastoma cells(Neuro 2A),we observed significant changes in the expression of numerous centrosomal *** manipulation also affected centrosome-related functions,including cell size and shape,cell proliferation,and ***,we found that CNKSR2 interactors were highly enriched in de novo variants associated with intellectual disability and autism spectrum *** findings establish a connection between CNKSR2 and the centrosome,and offer new insights into the underlying mechanisms of neurodevelopmental disorders.

关键词： autism spectrum disorder centrosome CNKSR2 intellectual disability interactome mass spectrometry microtubule neurodevelopmental disease protein complexes protein-protein interactions

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复杂条件微型市政盾构隧道施工关键技术研究

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中外公路 2020年第1期40卷 172-175页

作者：俞超杰刘浩然闵凡路河海大学岩土力学与堤坝工程教育部重点实验室江苏南京210098 河海大学土木与交通学院中铁十二局集团第七工程有限公司

南京某污水隧道工程采用直径2.8 m的泥浓式盾构,在穿越秦淮河下粉质黏土、风化粉砂质泥岩等软弱复合地层时,面临小竖井盾构始发、小半径曲线转弯、岸坡既有桩基拔除等难题,是目前中国典型的城区复杂条件下的微型盾构隧道工程。针对工程... 详细信息

南京某污水隧道工程采用直径2.8 m的泥浓式盾构,在穿越秦淮河下粉质黏土、风化粉砂质泥岩等软弱复合地层时,面临小竖井盾构始发、小半径曲线转弯、岸坡既有桩基拔除等难题,是目前中国典型的城区复杂条件下的微型盾构隧道工程。针对工程面临的技术难点,结合现场具体施工情况,采用筛分-压滤相结合的浓泥浆处理技术,解决了大量废弃泥浆处理问题,形成了小竖井盾构先推后盾分体始发、基于双主动铰接的小半径曲线转弯、旋转钻机配合套管钻头的桩基拔除等关键技术,提出了MMA底漆-瓷釉面涂的内壁防腐方案,可为今后类似工程建设提供参考。

关键词：微型盾构泥浓工法先推后盾桩基拔除内壁防腐

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颈椎融合术对邻近节段影响的研究进展

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医学综述 2016年第18期22卷 3599-3602页

作者：玉超杰梁斌广西壮族自治区人民医院骨科南宁530021

颈椎融合术是我国广泛应用于治疗各种颈椎疾病的常用手术方式之一。而术后邻近节段退变是颈椎融合术最常见的中远期并发症。因此,邻近节段退变的发病机制、影响因素等逐渐成为近年研究的热点。椎间盘置换术作为一种新的手术方式,可能减... 详细信息

颈椎融合术是我国广泛应用于治疗各种颈椎疾病的常用手术方式之一。而术后邻近节段退变是颈椎融合术最常见的中远期并发症。因此,邻近节段退变的发病机制、影响因素等逐渐成为近年研究的热点。椎间盘置换术作为一种新的手术方式,可能减少了邻近节段退变的发生。目前,邻近节段的发病机制尚未明确,通过分析其影响因素,在手术上开拓创新,有助于降低邻近节段退变的发生率。

关键词：颈椎融合术邻近节段退变椎间盘置换术

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